Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,310,422	T→C	D133G (GAC→GGC)	rbfA ←	ribosome‑binding factor A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,310,422	0	T	C	99.6%	944.6 / ‑4.7	277	D133G (GAC→GGC)	rbfA	ribosome‑binding factor A
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (157/117); total (159/118)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01

Only 100 of 293 total aligned reads displayed.

AGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACC  >  NC_000913/3310333‑3310511

aGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGccct                                                                                        >  1:2599054/1‑93 (MQ=255)

aGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGccct                                                                                        >  1:5800700/1‑93 (MQ=255)

  ggcATCCTTTCTGGACATTCCCTTAGGTTTAATCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGccctcc                                                                                      <  1:646171/92‑1 (MQ=255)

   gcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCcctcct                                                                                     >  2:2268920/1‑93 (MQ=255)

   gcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCcctcct                                                                                     >  2:6220727/1‑93 (MQ=255)

   gcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCcctcct                                                                                     <  2:1159326/93‑1 (MQ=255)

   gcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCcctcct                                                                                     <  2:1014596/93‑1 (MQ=255)

   gcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCcctcct                                                                                     <  1:4333604/93‑1 (MQ=255)

    cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCtt                                                                                    >  1:4943924/1‑93 (MQ=255)

    cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCtt                                                                                    >  2:1512099/1‑93 (MQ=255)

    cATCGTTGCTGGACATACCCTGAGGTTTATCCAACAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCtt                                                                                    >  1:3494413/1‑93 (MQ=255)

     ctCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTg                                                                                   <  1:2438282/92‑1 (MQ=255)

     aTCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTg                                                                                   >  2:5792724/1‑93 (MQ=255)

      tCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGc                                                                                  >  2:2379682/1‑93 (MQ=255)

          tGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTgtc                                                                              <  1:4979128/93‑1 (MQ=255)

           gCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTgtcg                                                                             >  1:1592158/1‑93 (MQ=255)

             tgcaCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTgtcgtc                                                                           <  2:6353985/90‑1 (MQ=255)

             tGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTgtcgtc                                                                           <  2:1190484/93‑1 (MQ=255)

              ggACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTcc                                                                          <  2:73416/93‑1 (MQ=255)

              ggACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTACTTGCTGTCGTcc                                                                          >  2:5111854/1‑93 (MQ=255)

                aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCgg                                                                        >  2:2210918/1‑93 (MQ=255)

                aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCgg                                                                        >  1:4772353/1‑93 (MQ=255)

                  aTACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGCGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGt                                                                      >  2:616535/1‑93 (MQ=255)

                    aCCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTa                                                                    >  2:3055137/1‑93 (MQ=255)

                    aCCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCATTGCTGTCGTCCGGGTTa                                                                    >  2:3416410/1‑93 (MQ=255)

                     cccTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTaa                                                                   >  1:3394454/1‑93 (MQ=255)

                     cccTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTaa                                                                   >  1:5691853/1‑93 (MQ=255)

                      ccTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAac                                                                  <  2:3616154/93‑1 (MQ=255)

                       cTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAaca                                                                 <  1:3334665/93‑1 (MQ=255)

                       cTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAaca                                                                 >  2:461957/1‑93 (MQ=255)

                         gAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCTGGTTAACacg                                                               <  1:3964274/93‑1 (MQ=255)

                         gAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACacg                                                               >  2:5668737/1‑93 (MQ=255)

                         gAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACacg                                                               >  1:5484085/1‑93 (MQ=255)

                          aGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACacga                                                              <  1:94749/93‑1 (MQ=255)

                           ggTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACacgac                                                             <  2:4452125/93‑1 (MQ=255)

                           ggTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACacgac                                                             <  2:1637127/93‑1 (MQ=255)

                               tATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGttc                                                         >  1:6344655/1‑93 (MQ=255)

                                agccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGttct                                                        <  2:5379536/91‑1 (MQ=255)

                                aTCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGttct                                                        >  2:4661308/1‑93 (MQ=255)

                                aTCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGttct                                                        >  2:5191341/1‑93 (MQ=255)

                                 tCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGttctt                                                       <  2:1853894/93‑1 (MQ=255)

                                   cAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCg                                                     <  1:5565784/93‑1 (MQ=255)

                                    aGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGt                                                    >  1:4662688/1‑93 (MQ=255)

                                      caacaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCa                                                  <  2:4807528/93‑1 (MQ=255)

                                      caacaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCa                                                  <  1:5322984/93‑1 (MQ=255)

                                       aacaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCAt                                                 <  2:3562709/93‑1 (MQ=255)

                                        acaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATg                                                <  1:751014/93‑1 (MQ=255)

                                          aaaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGtt                                              >  1:2834771/1‑93 (MQ=255)

                                          aaaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGtt                                              <  1:5097399/93‑1 (MQ=255)

                                          aaaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGtt                                              >  2:3708495/1‑93 (MQ=255)

                                          aaaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGtt                                              <  1:4029585/93‑1 (MQ=255)

                                           aaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGttt                                             <  2:1768581/93‑1 (MQ=255)

                                           aaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGttt                                             >  2:1583950/1‑93 (MQ=255)

                                             aCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGa                                           >  2:2564308/1‑93 (MQ=255)

                                               gCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGAcc                                         >  1:4286861/1‑93 (MQ=255)

                                                 cGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCAc                                       <  2:2664785/93‑1 (MQ=255)

                                                   ttAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGc                                     >  2:4919989/1‑93 (MQ=255)

                                                   ttAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCCTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGc                                     <  1:5245918/93‑1 (MQ=255)

                                                     aaTGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTg                                   <  1:5199231/93‑1 (MQ=255)

                                                      aTGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTgg                                  <  1:142339/93‑1 (MQ=255)

                                                       tGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGt                                 <  2:4242414/93‑1 (MQ=255)

                                                        gTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTc                                <  1:1859519/93‑1 (MQ=255)

                                                          cgcgaccgcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAc                              <  2:2855516/93‑1 (MQ=255)

                                                           gcgaccgcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAcc                             <  1:4844318/93‑1 (MQ=255)

                                                           gcgaccgcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAcc                             <  2:5863027/93‑1 (MQ=255)

                                                             caccgcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAg                           <  2:2083345/92‑1 (MQ=255)

                                                              accgcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAgg                          >  1:2039544/1‑93 (MQ=255)

                                                              accgcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAgg                          <  2:628136/93‑1 (MQ=255)

                                                               ccgcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGt                         <  1:4576125/93‑1 (MQ=255)

                                                               ccgcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGt                         <  1:4071773/93‑1 (MQ=255)

                                                                cgcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGtt                        <  2:6061306/93‑1 (MQ=255)

                                                                 gcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                       <  2:4553441/93‑1 (MQ=255)

                                                                 gcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                       >  1:1051836/1‑93 (MQ=255)

                                                                 gcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                       >  1:2702023/1‑93 (MQ=255)

                                                                 gcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                       >  1:1004758/1‑93 (MQ=255)

                                                                 gcgacGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                       <  1:3725946/93‑1 (MQ=255)

                                                                    acgacgaGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGAc                    >  2:1321485/1‑93 (MQ=255)

                                                                     cgacgaGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACa                   >  2:4096339/1‑93 (MQ=255)

                                                                     cgacgaGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACa                   <  1:2859893/93‑1 (MQ=255)

                                                                      gacgaGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAt                  >  1:6253436/1‑93 (MQ=255)

                                                                       acgaGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                 >  2:4004743/1‑93 (MQ=255)

                                                                       acgaGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                 >  2:2895935/1‑93 (MQ=255)

                                                                         gaGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATgcg               >  1:1964540/1‑93 (MQ=255)

                                                                         gaGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATgcg               >  1:123026/1‑93 (MQ=255)

                                                                          aGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATgcgc              >  2:5620888/1‑93 (MQ=255)

                                                                           ggACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATgcgcc             >  1:5918045/1‑92 (MQ=255)

                                                                           ggACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCa             >  1:3021436/1‑93 (MQ=255)

                                                                           ggACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCa             >  2:2481176/1‑93 (MQ=255)

                                                                              cgacTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATcc          >  1:1686083/1‑93 (MQ=255)

                                                                              cgacTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATcc          <  1:1320016/93‑1 (MQ=255)

                                                                              cgacTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATcc          <  1:4522823/93‑1 (MQ=255)

                                                                                acTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCt        <  1:2352039/93‑1 (MQ=255)

                                                                                acTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCt        <  2:5974830/93‑1 (MQ=255)

                                                                                   cATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCa     >  2:2784622/1‑93 (MQ=255)

                                                                                    cttAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCaa    <  1:3584566/92‑1 (MQ=255)

                                                                                    aTTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCaa    >  2:4331751/1‑93 (MQ=255)

                                                                                     ttAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAc   >  2:3788503/1‑93 (MQ=255)

                                                                                     ttAGCCCTCCTTGCTGTCGACCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAc   >  1:6238705/1‑93 (MQ=255)

                                                                                      tAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAcc  <  1:3152589/93‑1 (MQ=255)

                                                                                      tAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAcc  <  1:5128623/93‑1 (MQ=255)

AGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACC  >  NC_000913/3310333‑3310511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAG  >  NC_000913/3310329‑3310515

TTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGC                                                                                               >  SRR4302534.5853246/1‑94 (MQ=60)

TTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGC                                                                                               <  SRR4302534.6390757/94‑1 (MQ=60)

 TGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCC                                                                                              >  SRR4302534.1601755/1‑94 (MQ=60)

  GCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCC                                                                                             <  SRR4302534.2665717/94‑1 (MQ=60)

   CAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCT                                                                                            >  SRR4302534.3480874/1‑94 (MQ=60)

   CAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCT                                                                                            >  SRR4302534.2667832/1‑94 (MQ=60)

   CAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCT                                                                                            >  SRR4302534.5958121/1‑94 (MQ=60)

    AGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTC                                                                                           <  SRR4302534.5343257/94‑1 (MQ=60)

    AGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTC                                                                                           >  SRR4302534.1330644/1‑94 (MQ=60)

    AGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTC                                                                                           >  SRR4302534.1613173/1‑94 (MQ=60)

      ggcatcctttCTGGACATTCCCTTAGGTTTAATCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCT                                                                                         <  SRR4302534.661836/84‑1 (MQ=60)

      CGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCT                                                                                         <  SRR4302534.3582641/94‑1 (MQ=60)

      CGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCT                                                                                         >  SRR4302534.4765462/1‑94 (MQ=60)

      CGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCT                                                                                         <  SRR4302534.5060176/94‑1 (MQ=60)

       GCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTT                                                                                        >  SRR4302534.2936816/1‑94 (MQ=60)

       GCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTT                                                                                        >  SRR4302534.5077871/1‑94 (MQ=60)

       GCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTT                                                                                        <  SRR4302534.4450767/94‑1 (MQ=60)

       GCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTT                                                                                        <  SRR4302534.831580/94‑1 (MQ=60)

       GCATCGTTGCTGGACATACCCTGAGGTTTATCCAACAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTT                                                                                        >  SRR4302534.3588362/1‑94 (MQ=60)

         CTCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGC                                                                                      <  SRR4302534.2503082/94‑1 (MQ=60)

         ATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGC                                                                                      >  SRR4302534.5288558/1‑94 (MQ=60)

          TCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCT                                                                                     >  SRR4302534.5905363/1‑94 (MQ=60)

          TCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCT                                                                                     <  SRR4302534.4687588/94‑1 (MQ=60)

           CGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGGCCTCCTTGCTG                                                                                    >  SRR4302534.6416038/1‑94 (MQ=60)

             TTGCTGGACATACCCTGAGGTTTATCCAGCAATAAAACGCCGTTAATGTCGCGACCGGGACGACGAGGATGCCTCATTAGCCCTCCTTGCTGCC                                                                                  >  SRR4302534.3980697/1‑94 (MQ=60)

             TTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTG                                                                                  >  SRR4302534.4977466/1‑94 (MQ=60)

              TGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCG                                                                                 <  SRR4302534.5113900/94‑1 (MQ=60)

              TGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCG                                                                                 <  SRR4302534.6653893/94‑1 (MQ=60)

              TGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCG                                                                                 >  SRR4302534.5338802/1‑94 (MQ=60)

              TGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCG                                                                                 >  SRR4302534.1633789/1‑94 (MQ=60)

               GCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGT                                                                                <  SRR4302534.3658487/94‑1 (MQ=60)

                   GACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGG                                                                            >  SRR4302534.4901926/1‑94 (MQ=60)

                   GACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGG                                                                            >  SRR4302534.4929372/1‑94 (MQ=60)

                    ACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGG                                                                           <  SRR4302534.5453128/94‑1 (MQ=60)

                    ACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGG                                                                           >  SRR4302534.5135802/1‑94 (MQ=60)

                      ATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTT                                                                         <  SRR4302534.3038362/94‑1 (MQ=60)

                      ATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTT                                                                         >  SRR4302534.4272677/1‑94 (MQ=60)

                        ACCCTGAGGTTTATCCAGCAACAATACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTGGCCCTCCTTGCTGTCGTCCGGGTTAA                                                                       >  SRR4302534.5342044/1‑94 (MQ=60)

                        ACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAA                                                                       >  SRR4302534.3485536/1‑94 (MQ=60)

                        ACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAA                                                                       >  SRR4302534.2759656/1‑94 (MQ=60)

                        ACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAA                                                                       >  SRR4302534.5846014/1‑94 (MQ=60)

                        ACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAA                                                                       >  SRR4302534.417156/1‑94 (MQ=60)

                          CCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACA                                                                     >  SRR4302534.1410903/1‑94 (MQ=60)

                           CTGAGGTTTATCCAGCAACAAAACGCCGTTGATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACAC                                                                    <  SRR4302534.5694465/94‑1 (MQ=60)

                           CTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACAC                                                                    <  SRR4302534.3423879/94‑1 (MQ=60)

                            TGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACG                                                                   >  SRR4302534.5631998/1‑94 (MQ=60)

                            TGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACG                                                                   <  SRR4302534.6024143/94‑1 (MQ=60)

                             GAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCTGGTTAACACGA                                                                  <  SRR4302534.4070406/94‑1 (MQ=60)

                              AGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGAC                                                                 <  SRR4302534.179323/94‑1 (MQ=60)

                              AGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGAC                                                                 <  SRR4302534.97280/94‑1 (MQ=60)

                               GGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACG                                                                <  SRR4302534.434594/94‑1 (MQ=60)

                                 TTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTT                                                              <  SRR4302534.6074732/94‑1 (MQ=60)

                                 TATATCCACCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTT                                                              <  SRR4302534.2532577/94‑1 (MQ=60)

                                  TTATCCAGCAATAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTC                                                             <  SRR4302534.2298738/94‑1 (MQ=60)

                                  TTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTC                                                             <  SRR4302534.4107552/94‑1 (MQ=60)

                                  TTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTC                                                             >  SRR4302534.6516010/1‑94 (MQ=60)

                                   TATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCT                                                            <  SRR4302534.3260210/94‑1 (MQ=60)

                                   TATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCT                                                            >  SRR4302534.3681175/1‑94 (MQ=60)

                                    ATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCTCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTT                                                           >  SRR4302534.4530962/1‑94 (MQ=60)

                                    ATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTT                                                           <  SRR4302534.4905065/94‑1 (MQ=60)

                                     TCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTC                                                          >  SRR4302534.3485682/1‑94 (MQ=60)

                                     TCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTC                                                          <  SRR4302534.5468068/94‑1 (MQ=60)

                                     TCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTC                                                          <  SRR4302534.2441772/94‑1 (MQ=60)

                                       CAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGT                                                        >  SRR4302534.4789284/1‑94 (MQ=60)

                                       CAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGT                                                        <  SRR4302534.5716083/94‑1 (MQ=60)

                                       CAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGT                                                        >  SRR4302534.2236568/1‑94 (MQ=60)

                                        AGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTC                                                       <  SRR4302534.6486835/94‑1 (MQ=60)

                                         GCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCA                                                      >  SRR4302534.5246595/1‑94 (MQ=60)

                                          CAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCAT                                                     <  SRR4302534.6571603/94‑1 (MQ=60)

                                          CAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCAT                                                     >  SRR4302534.1951829/1‑94 (MQ=60)

                                          CAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCAT                                                     <  SRR4302534.5466557/94‑1 (MQ=60)

                                           AACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATG                                                    >  SRR4302534.4427413/1‑94 (MQ=60)

                                           AACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATG                                                    <  SRR4302534.1007178/94‑1 (MQ=60)

                                            ACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGT                                                   >  SRR4302534.6895/1‑94 (MQ=60)

                                            ACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGT                                                   <  SRR4302534.769622/94‑1 (MQ=60)

                                             CAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTT                                                  >  SRR4302534.2909246/1‑94 (MQ=60)

                                             CAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTT                                                  <  SRR4302534.5092107/94‑1 (MQ=60)

                                              AAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTT                                                 <  SRR4302534.4137625/94‑1 (MQ=60)

                                              AAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTT                                                 <  SRR4302534.5234976/94‑1 (MQ=60)

                                               AAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTG                                                >  SRR4302534.359540/1‑94 (MQ=60)

                                                AACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGA                                               <  SRR4302534.5146185/94‑1 (MQ=60)

                                                AACGCCGTTAATGTCGCGACCGCGACGACGAGGACCACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGA                                               >  SRR4302534.3976404/1‑94 (MQ=60)

                                                AACGCCGTGAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGA                                               <  SRR4302534.4783946/94‑1 (MQ=60)

                                                  CGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACC                                             >  SRR4302534.4402995/1‑94 (MQ=60)

                                                  CGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACC                                             >  SRR4302534.3907560/1‑94 (MQ=60)

                                                   GCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCA                                            <  SRR4302534.2809866/94‑1 (MQ=60)

                                                     CGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACG                                          >  SRR4302534.640209/1‑94 (MQ=60)

                                                       TTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCCTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCT                                        <  SRR4302534.5387613/94‑1 (MQ=60)

                                                        TAATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTTACACGACGTTCTTCGTCATGTTTGACCACGCTG                                       >  SRR4302534.560449/1‑94 (MQ=60)

                                                         AATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGG                                      <  SRR4302534.5339477/94‑1 (MQ=60)

                                                         AATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGG                                      <  SRR4302534.1040960/94‑1 (MQ=60)

                                                          ATGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGT                                     <  SRR4302534.146062/94‑1 (MQ=60)

                                                           TGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTC                                    >  SRR4302534.5102655/1‑94 (MQ=60)

                                                           TGTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTC                                    <  SRR4302534.1530469/94‑1 (MQ=60)

                                                            GTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCA                                   <  SRR4302534.1907920/94‑1 (MQ=60)

                                                            GTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCA                                   >  SRR4302534.5740166/1‑94 (MQ=60)

                                                            GTCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCA                                   <  SRR4302534.6376091/94‑1 (MQ=60)

                                                             TCGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAC                                  <  SRR4302534.563581/94‑1 (MQ=60)

                                                              CGCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACC                                 >  SRR4302534.1133206/1‑94 (MQ=60)

                                                               GCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCA                                >  SRR4302534.317350/1‑94 (MQ=60)

                                                               GCGACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCA                                <  SRR4302534.4975756/94‑1 (MQ=60)

                                                                 GACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGG                              >  SRR4302534.2092868/1‑94 (MQ=60)

                                                                 GACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGG                              >  SRR4302534.4848293/1‑94 (MQ=60)

                                                                 GACCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGG                              >  SRR4302534.5816206/1‑94 (MQ=60)

                                                                   CCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTT                            <  SRR4302534.4181035/94‑1 (MQ=60)

                                                                   CCGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTT                            <  SRR4302534.4700227/94‑1 (MQ=60)

                                                                    CGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTT                           >  SRR4302534.2773393/1‑94 (MQ=60)

                                                                    CGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTT                           >  SRR4302534.1030531/1‑94 (MQ=60)

                                                                    CGCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTT                           >  SRR4302534.1079066/1‑94 (MQ=60)

                                                                     GCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTG                          >  SRR4302534.4441812/1‑94 (MQ=60)

                                                                     GCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTG                          <  SRR4302534.3826095/94‑1 (MQ=60)

                                                                     CCGACGACGAGGACGACTCATTAGCCCTCCTTGCTGACGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTG                          <  SRR4302534.1189839/94‑1 (MQ=60)

                                                                      CGACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGA                         >  SRR4302534.2563152/1‑94 (MQ=60)

                                                                       GACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGAC                        >  SRR4302534.1873385/1‑94 (MQ=60)

                                                                        ACGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACA                       <  SRR4302534.6314470/94‑1 (MQ=60)

                                                                         CGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAT                      <  SRR4302534.2935015/94‑1 (MQ=60)

                                                                         CGACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAT                      >  SRR4302534.6422951/1‑94 (MQ=60)

                                                                          GACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTTACCAGGTTTGACATG                     >  SRR4302534.4128512/1‑94 (MQ=60)

                                                                          GACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATG                     >  SRR4302534.2008013/1‑94 (MQ=60)

                                                                           ACGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGC                    >  SRR4302534.4895501/1‑94 (MQ=60)

                                                                            CGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCG                   >  SRR4302534.1322414/1‑94 (MQ=60)

                                                                            CGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCG                   >  SRR4302534.2015969/1‑94 (MQ=60)

                                                                            CGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCG                   >  SRR4302534.2038172/1‑94 (MQ=60)

                                                                            CGAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCG                   >  SRR4302534.126277/1‑94 (MQ=60)

                                                                             GAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGC                  >  SRR4302534.3478806/1‑94 (MQ=60)

                                                                             GAGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGC                  >  SRR4302534.3172790/1‑94 (MQ=60)

                                                                              AGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCC                 >  SRR4302534.6078733/1‑94 (MQ=60)

                                                                              AGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCA                 >  SRR4302534.2040544/1‑94 (MQ=60)

                                                                              AGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCA                 >  SRR4302534.2741795/1‑94 (MQ=60)

                                                                              AGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCA                 >  SRR4302534.2801481/1‑94 (MQ=60)

                                                                              AGGACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCA                 >  SRR4302534.3100573/1‑94 (MQ=60)

                                                                                GACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATC               >  SRR4302534.2477026/1‑94 (MQ=60)

                                                                                 ACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCC              >  SRR4302534.1642116/1‑94 (MQ=60)

                                                                                 ACGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCC              >  SRR4302534.1729927/1‑94 (MQ=60)

                                                                                  CGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCC             >  SRR4302534.5244400/1‑94 (MQ=60)

                                                                                  CGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCC             <  SRR4302534.4645346/94‑1 (MQ=60)

                                                                                  CGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCC             >  SRR4302534.1436572/1‑94 (MQ=60)

                                                                                  CGACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCC             <  SRR4302534.1354824/94‑1 (MQ=60)

                                                                                   GACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGCCGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCT            <  SRR4302534.6548497/94‑1 (MQ=60)

                                                                                    ACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTT           <  SRR4302534.4613510/94‑1 (MQ=60)

                                                                                    ACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTT           <  SRR4302534.2414466/94‑1 (MQ=60)

                                                                                    ACTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTT           <  SRR4302534.928436/94‑1 (MQ=60)

                                                                                     CTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTTGTCACCAGGTTTGACATGCGCATCCCTTC          <  SRR4302534.1723075/94‑1 (MQ=60)

                                                                                     CTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTC          >  SRR4302534.6438740/1‑94 (MQ=60)

                                                                                     CTCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTC          <  SRR4302534.3322081/94‑1 (MQ=60)

                                                                                      TCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCA         >  SRR4302534.1005399/1‑94 (MQ=60)

                                                                                      TCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCA         <  SRR4302534.2929115/94‑1 (MQ=60)

                                                                                      TCATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCA         <  SRR4302534.4156222/94‑1 (MQ=60)

                                                                                        CTTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAC       <  SRR4302534.3680988/94‑1 (MQ=60)

                                                                                        ATTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAC       >  SRR4302534.5465191/1‑94 (MQ=60)

                                                                                        ATTAGCCCTCCTTGCTGTCGACCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAC       >  SRR4302534.6408007/1‑94 (MQ=60)

                                                                                         TTAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACC      >  SRR4302534.3692263/1‑94 (MQ=60)

                                                                                          TAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCA     <  SRR4302534.5267087/94‑1 (MQ=60)

                                                                                          TAGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCA     <  SRR4302534.3235604/94‑1 (MQ=60)

                                                                                           AGCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAG    >  SRR4302534.3357866/1‑94 (MQ=60)

                                                                                            GCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGA   <  SRR4302534.6047840/94‑1 (MQ=60)

                                                                                            GCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGA   <  SRR4302534.2368807/94‑1 (MQ=60)

                                                                                            GCCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGA   <  SRR4302534.3998962/94‑1 (MQ=60)

                                                                                             CCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAG  <  SRR4302534.4141990/94‑1 (MQ=60)

TTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAG  >  NC_000913/3310329‑3310515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`