Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,738,723 A→G intergenic (+136/+15) avtA → / ← yiaI alanine‑alpha‑ketoisovalerate transaminase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,738,7230AG100.0% 905.6 / NA 259intergenic (+136/+15)avtA/yiaIalanine‑alpha‑ketoisovalerate transaminase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (148/111);  total (148/111)

Only 100 of 268 total aligned reads displayed.

AATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCA  >  NC_000913/3738633‑3738812
                                                                                          |                                                                                         
aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCg                                                                                         >  1:681082/1‑93 (MQ=255)
aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCg                                                                                         <  2:2549692/93‑1 (MQ=255)
aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCg                                                                                         >  1:2078533/1‑93 (MQ=255)
  tatatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGcc                                                                                       <  2:1085074/93‑1 (MQ=255)
  tatatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGcc                                                                                       <  1:2032818/93‑1 (MQ=255)
    tatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCgg                                                                                     >  1:3824400/1‑93 (MQ=255)
    tatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCgg                                                                                     >  1:4957779/1‑93 (MQ=255)
     atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGa                                                                                    >  1:1188261/1‑93 (MQ=255)
     atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGa                                                                                    >  1:6249033/1‑93 (MQ=255)
     atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGa                                                                                    >  1:4936601/1‑93 (MQ=255)
     atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGa                                                                                    >  2:4357270/1‑93 (MQ=255)
     atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGa                                                                                    >  1:1846608/1‑93 (MQ=255)
        gAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGaaaa                                                                                 >  2:3286112/1‑93 (MQ=255)
         aaTTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAAc                                                                                <  2:5143572/93‑1 (MQ=255)
         aaTTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAAc                                                                                <  2:6441067/93‑1 (MQ=255)
          aTTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACg                                                                               >  1:4393405/1‑93 (MQ=255)
            ttGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGtt                                                                             <  2:2918226/93‑1 (MQ=255)
             tGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGttt                                                                            >  1:5578120/1‑93 (MQ=255)
               gaagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCa                                                                          >  1:3014003/1‑93 (MQ=255)
                aagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCaa                                                                         >  1:5392713/1‑93 (MQ=255)
                  gaaTTTGTAGTCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAATGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAAc                                                                       <  1:4213576/93‑1 (MQ=255)
                   aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACa                                                                      <  1:4068871/93‑1 (MQ=255)
                   aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACa                                                                      <  2:4043811/93‑1 (MQ=255)
                   aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACa                                                                      >  2:1626807/1‑93 (MQ=255)
                     tttGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATGAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAgg                                                                    >  2:4641945/1‑93 (MQ=255)
                     tttGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAgg                                                                    <  2:2059671/93‑1 (MQ=255)
                       tGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGgct                                                                  <  2:525088/93‑1 (MQ=255)
                         tAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGgctgc                                                                >  1:900744/1‑93 (MQ=255)
                          aGGCCGGATAAGGCGTTTACGCCGGATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGgctgct                                                               >  1:6223289/1‑93 (MQ=255)
                          aGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGgctgct                                                               <  1:6257872/93‑1 (MQ=255)
                           ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGctgctg                                                              >  2:5596308/1‑93 (MQ=255)
                           ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGctgctg                                                              <  1:1522362/93‑1 (MQ=255)
                              cGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCg                                                           >  2:6007471/1‑93 (MQ=255)
                               ggATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCgg                                                          >  1:159505/1‑92 (MQ=255)
                               ggATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGt                                                          >  2:694546/1‑93 (MQ=255)
                               ggATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGt                                                          <  2:3600297/93‑1 (MQ=255)
                               ggATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGt                                                          <  2:5855057/93‑1 (MQ=255)
                                gATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGtg                                                         >  1:2859994/1‑92 (MQ=255)
                                gATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTa                                                         <  1:2931947/93‑1 (MQ=255)
                                gATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTa                                                         >  1:2951955/1‑93 (MQ=255)
                                 aTAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGGAg                                                        >  2:1423415/1‑93 (MQ=255)
                                  tAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGc                                                       <  2:5329418/93‑1 (MQ=255)
                                    aGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAACCg                                                     >  2:3183517/1‑93 (MQ=255)
                                     ggCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGa                                                    <  2:380957/93‑1 (MQ=255)
                                      gCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGAt                                                   <  1:1755680/93‑1 (MQ=255)
                                      gCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGAt                                                   >  1:1444358/1‑93 (MQ=255)
                                         tttACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGcc                                                >  2:838568/1‑93 (MQ=255)
                                          ttACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCg                                               >  2:2198221/1‑93 (MQ=255)
                                           tACGCCTCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACATGCTGCTGCCGTAGCCGATGCCgt                                              >  1:5305933/1‑92 (MQ=39)
                                           tACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGc                                              >  1:218743/1‑93 (MQ=255)
                                             cgccgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTg                                            <  1:2868290/93‑1 (MQ=255)
                                               ccgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCa                                          >  2:4982031/1‑93 (MQ=255)
                                                cgcATCTGGCATAAACAAGGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCAc                                         <  2:5488568/93‑1 (MQ=255)
                                                cgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCAc                                         <  2:2659201/93‑1 (MQ=255)
                                                cgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCAc                                         <  2:6246148/93‑1 (MQ=255)
                                                cgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCAc                                         >  1:1366544/1‑93 (MQ=255)
                                                 gcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACt                                        >  1:601796/1‑93 (MQ=255)
                                                  cATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                       >  2:2987333/1‑93 (MQ=255)
                                                  cATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                       >  1:4706135/1‑93 (MQ=255)
                                                  cATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                       <  1:3971186/93‑1 (MQ=255)
                                                   aTCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACttt                                      >  1:5805964/1‑93 (MQ=255)
                                                   aTCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACttt                                      <  1:2596318/93‑1 (MQ=255)
                                                    tCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTtc                                     >  1:5212313/1‑93 (MQ=255)
                                                    tCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTtc                                     <  1:1672600/93‑1 (MQ=255)
                                                     cTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTtct                                    <  1:5345101/93‑1 (MQ=255)
                                                       ggCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCg                                  >  2:503235/1‑93 (MQ=255)
                                                       ggCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCg                                  >  1:887994/1‑93 (MQ=255)
                                                       ggCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCg                                  <  2:243510/93‑1 (MQ=255)
                                                       ggCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCg                                  >  1:2771336/1‑93 (MQ=255)
                                                        gCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGa                                 <  1:1646369/93‑1 (MQ=255)
                                                        gCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGa                                 <  2:5314980/93‑1 (MQ=255)
                                                        gCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGa                                 <  2:6337916/93‑1 (MQ=255)
                                                          aTAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACa                               >  1:3402724/1‑93 (MQ=255)
                                                           tAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACAt                              >  2:4140945/1‑93 (MQ=255)
                                                             aaCAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATcc                            >  2:5530242/1‑93 (MQ=255)
                                                              aCAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                           >  1:3517451/1‑93 (MQ=255)
                                                               cAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCAt                          <  1:4069546/93‑1 (MQ=255)
                                                                aaaGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATg                         >  1:1011455/1‑93 (MQ=255)
                                                                aaaGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATg                         <  1:564344/93‑1 (MQ=255)
                                                                  aGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCa                       >  2:47092/1‑93 (MQ=255)
                                                                     gcACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTg                    >  1:3658113/1‑93 (MQ=255)
                                                                     gcACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCAGCTGCACTTTCTCGACATCCATACACTg                    >  2:4826057/1‑93 (MQ=255)
                                                                      cACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGc                   >  1:5608332/1‑93 (MQ=255)
                                                                         tGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAAc                >  2:1713830/1‑93 (MQ=255)
                                                                          gTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAAcc               >  2:2845253/1‑92 (MQ=255)
                                                                           ttCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGc              >  1:2550000/1‑93 (MQ=255)
                                                                           ttCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGc              >  2:2277001/1‑93 (MQ=255)
                                                                              cGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCtt           <  2:5690488/93‑1 (MQ=255)
                                                                               ggCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCttt          >  2:1836751/1‑93 (MQ=255)
                                                                                 cGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGt        <  2:6395635/93‑1 (MQ=255)
                                                                                 cGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGt        <  2:3108710/93‑1 (MQ=255)
                                                                                  gTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTc       <  1:6383931/93‑1 (MQ=255)
                                                                                    tGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCgg     >  2:3765460/1‑93 (MQ=255)
                                                                                     gagaAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCggg    >  2:4560083/1‑93 (MQ=255)
                                                                                     gagaAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCggg    <  2:4189262/93‑1 (MQ=255)
                                                                                     gagaAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCggg    >  1:1045154/1‑93 (MQ=255)
                                                                                      agaAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGc   >  1:1882149/1‑93 (MQ=255)
                                                                                      agaAGCCCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGc   <  2:618035/93‑1 (MQ=255)
                                                                                       gaAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCa  >  2:1233856/1‑93 (MQ=255)
                                                                                       gaAGCGCCGGAAAACGTTTCAAACACCCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATTCATGCACTGCAACGCCTCTGTCGGGcg  >  1:4243364/1‑92 (MQ=255)
                                                                                          |                                                                                         
AATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCA  >  NC_000913/3738633‑3738812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCT  >  NC_000913/3738630‑3738816
                                                                                             |                                                                                             
TGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAG                                                                                               <  SRR4302534.1972817/94‑1 (MQ=60)
TGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAG                                                                                               >  SRR4302534.5878933/1‑94 (MQ=60)
 GCAATATATTGAATTTGGAAGAAGTTGTAGGCCGGATAAGGAGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGC                                                                                              <  SRR4302534.55107/94‑1 (MQ=60)
  CAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCG                                                                                             >  SRR4302534.2132965/1‑94 (MQ=60)
  CAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCG                                                                                             <  SRR4302534.6200543/94‑1 (MQ=60)
  CAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCG                                                                                             <  SRR4302534.6529768/94‑1 (MQ=60)
  CAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCG                                                                                             >  SRR4302534.697716/1‑94 (MQ=60)
    ATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCC                                                                                           >  SRR4302534.5476553/1‑94 (MQ=60)
     TATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCG                                                                                          <  SRR4302534.2085973/94‑1 (MQ=60)
      ATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGG                                                                                         >  SRR4302534.2079264/1‑94 (MQ=60)
      ATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGG                                                                                         <  SRR4302534.2050128/94‑1 (MQ=60)
      ATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGG                                                                                         >  SRR4302534.3927134/1‑94 (MQ=60)
      ATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGG                                                                                         <  SRR4302534.3680901/94‑1 (MQ=60)
      ATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGG                                                                                         >  SRR4302534.170274/1‑94 (MQ=60)
      ATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGG                                                                                         >  SRR4302534.5092037/1‑94 (MQ=60)
       TATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGA                                                                                        >  SRR4302534.3957299/1‑94 (MQ=60)
       TATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGA                                                                                        <  SRR4302534.5299890/94‑1 (MQ=60)
       TATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGA                                                                                        >  SRR4302534.1894647/1‑94 (MQ=60)
       TATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGA                                                                                        >  SRR4302534.5070379/1‑94 (MQ=60)
       TATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGA                                                                                        >  SRR4302534.1219506/1‑94 (MQ=60)
       TATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGA                                                                                        >  SRR4302534.6418495/1‑94 (MQ=60)
          TGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAA                                                                                     <  SRR4302534.5400394/94‑1 (MQ=60)
            AATTTGGAAGAATTTGTAGTCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACGGTTCCGGCGTTGAGAAGCGCCGGAAAACG                                                                                   <  SRR4302534.1402898/94‑1 (MQ=60)
            AATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACG                                                                                   >  SRR4302534.3014636/1‑94 (MQ=60)
            AATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACG                                                                                   <  SRR4302534.6621500/94‑1 (MQ=60)
            AATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACG                                                                                   >  SRR4302534.4511892/1‑94 (MQ=60)
               TTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTT                                                                                <  SRR4302534.5490373/94‑1 (MQ=60)
               TTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTT                                                                                >  SRR4302534.5728799/1‑94 (MQ=60)
                TGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTC                                                                               >  SRR4302534.1602975/1‑94 (MQ=60)
                 GGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCA                                                                              >  SRR4302534.3092889/1‑94 (MQ=60)
                  GAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAA                                                                             >  SRR4302534.5537926/1‑94 (MQ=60)
                    AGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAAC                                                                           >  SRR4302534.2127954/1‑94 (MQ=60)
                     GAATTTGTAGTCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAATGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACA                                                                          <  SRR4302534.4327224/94‑1 (MQ=60)
                      AATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAG                                                                         <  SRR4302534.4178050/94‑1 (MQ=60)
                       ATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGG                                                                        <  SRR4302534.4844236/94‑1 (MQ=60)
                       ATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGG                                                                        >  SRR4302534.1166876/1‑94 (MQ=60)
                       ATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGG                                                                        >  SRR4302534.5436311/1‑94 (MQ=60)
                          TGTAGGCGGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTG                                                                     <  SRR4302534.1042106/94‑1 (MQ=60)
                          TGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTG                                                                     >  SRR4302534.1802772/1‑94 (MQ=60)
                          TGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTG                                                                     >  SRR4302534.286736/1‑94 (MQ=60)
                           GTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGC                                                                    >  SRR4302534.4178624/1‑94 (MQ=60)
                           GTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGC                                                                    >  SRR4302534.923581/1‑94 (MQ=60)
                            TAGGCCGGTTAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGACAACGTTTCAAACAGGCTGAT                                                                   >  SRR4302534.2136460/1‑94 (MQ=60)
                            TAGGCCGGATAAGGCGTTTACGCCGGATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCT                                                                   >  SRR4302534.6392285/1‑94 (MQ=60)
                            TAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCT                                                                   >  SRR4302534.2826858/1‑94 (MQ=60)
                             AGGCCGGATAAGGCGTTTGCGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTG                                                                  >  SRR4302534.5622398/1‑94 (MQ=60)
                             AGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTG                                                                  <  SRR4302534.6427449/94‑1 (MQ=60)
                             AGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTG                                                                  <  SRR4302534.5229479/94‑1 (MQ=60)
                              GGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGC                                                                 <  SRR4302534.1562329/94‑1 (MQ=60)
                               GCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCC                                                                <  SRR4302534.4317114/94‑1 (MQ=60)
                                 CGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGT                                                              >  SRR4302534.5090584/1‑94 (MQ=60)
                                 CGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGG                                                              >  SRR4302534.163676/1‑94 (MQ=60)
                                  GGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTG                                                             >  SRR4302534.2935118/1‑94 (MQ=60)
                                  GGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTA                                                             >  SRR4302534.3029355/1‑94 (MQ=60)
                                   GATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAG                                                            >  SRR4302534.5863929/1‑94 (MQ=60)
                                   GATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAG                                                            <  SRR4302534.3008915/94‑1 (MQ=60)
                                    ATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGC                                                           >  SRR4302534.151337/1‑94 (MQ=60)
                                     TAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCC                                                          <  SRR4302534.1757869/94‑1 (MQ=60)
                                     TAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCC                                                          <  SRR4302534.3793860/94‑1 (MQ=60)
                                      AAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCG                                                         >  SRR4302534.6049213/1‑94 (MQ=60)
                                      AAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCG                                                         >  SRR4302534.435259/1‑94 (MQ=60)
                                        GGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGAT                                                       <  SRR4302534.4153631/94‑1 (MQ=60)
                                        GGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGAT                                                       >  SRR4302534.1482374/1‑94 (MQ=60)
                                         GCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATG                                                      <  SRR4302534.1801274/94‑1 (MQ=60)
                                         GCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACACGCTGCTGCCGTAGCGGATG                                                      >  SRR4302534.4742934/1‑94 (MQ=60)
                                          CGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGC                                                     >  SRR4302534.3412229/1‑94 (MQ=60)
                                          CGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGC                                                     >  SRR4302534.5789686/1‑94 (MQ=60)
                                          CGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGGTTCAAACAGGCTGCTGCCGTAGCCGATGC                                                     >  SRR4302534.6523865/1‑94 (MQ=60)
                                           GTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCC                                                    >  SRR4302534.1734622/1‑94 (MQ=60)
                                            TTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCAG                                                   >  SRR4302534.1511703/1‑94 (MQ=60)
                                             TTACGCCTCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACATGCTGCTGCCGTAGCCGATGCCGT                                                  >  SRR4302534.5449091/1‑94 (MQ=60)
                                             TTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGC                                                  >  SRR4302534.224478/1‑94 (MQ=60)
                                              TACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCT                                                 <  SRR4302534.629018/94‑1 (MQ=60)
                                              TACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCT                                                 >  SRR4302534.2637094/1‑94 (MQ=60)
                                               ACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTG                                                <  SRR4302534.640796/94‑1 (MQ=60)
                                                CGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGC                                               <  SRR4302534.2943611/94‑1 (MQ=60)
                                                CGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGC                                               >  SRR4302534.6345971/1‑94 (MQ=60)
                                                 GCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCA                                              <  SRR4302534.3939527/94‑1 (MQ=60)
                                                  CCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCAC                                             >  SRR4302534.1402535/1‑94 (MQ=60)
                                                   CGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACT                                            <  SRR4302534.1608464/94‑1 (MQ=60)
                                                   CGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACT                                            >  SRR4302534.616460/1‑94 (MQ=60)
                                                   CGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACT                                            <  SRR4302534.1319062/94‑1 (MQ=60)
                                                    GCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTT                                           >  SRR4302534.4833889/1‑94 (MQ=60)
                                                    GCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTT                                           >  SRR4302534.1637899/1‑94 (MQ=60)
                                                     CATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTT                                          <  SRR4302534.4077480/94‑1 (MQ=60)
                                                     CATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTT                                          >  SRR4302534.5963540/1‑94 (MQ=60)
                                                     CATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTT                                          >  SRR4302534.83133/1‑94 (MQ=60)
                                                      ATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTC                                         >  SRR4302534.5352985/1‑94 (MQ=60)
                                                      ATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTC                                         <  SRR4302534.2665026/94‑1 (MQ=60)
                                                       TCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCT                                        <  SRR4302534.1716125/94‑1 (MQ=60)
                                                       TCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCT                                        >  SRR4302534.4348291/1‑94 (MQ=60)
                                                       TCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCT                                        <  SRR4302534.4635662/94‑1 (MQ=60)
                                                        CTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTC                                       <  SRR4302534.5489217/94‑1 (MQ=60)
                                                         TGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCG                                      >  SRR4302534.639770/1‑94 (MQ=60)
                                                         TGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCG                                      >  SRR4302534.2844260/1‑94 (MQ=60)
                                                         TGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCG                                      >  SRR4302534.910476/1‑94 (MQ=60)
                                                           GCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAC                                    >  SRR4302534.4105348/1‑94 (MQ=60)
                                                           GCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAC                                    <  SRR4302534.1689285/94‑1 (MQ=60)
                                                            CATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGCAAACGTTTCAAACAGGCTGGTGCCGTGGCCGATGACGATGCACTTTGTCGACG                                   >  SRR4302534.5566532/1‑94 (MQ=60)
                                                            CATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACA                                   >  SRR4302534.3494089/1‑94 (MQ=60)
                                                            CATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACA                                   >  SRR4302534.742781/1‑94 (MQ=60)
                                                             ATAAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACAT                                  >  SRR4302534.5843388/1‑94 (MQ=60)
                                                               AAACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCC                                <  SRR4302534.1925053/94‑1 (MQ=60)
                                                                AACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCA                               >  SRR4302534.3612060/1‑94 (MQ=60)
                                                                 ACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCAT                              >  SRR4302534.1699750/1‑94 (MQ=60)
                                                                 ACAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCAT                              >  SRR4302534.286356/1‑94 (MQ=60)
                                                                 ACAAAGCGCACTGTTCCGGCGTGGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCAT                              <  SRR4302534.64673/94‑1 (MQ=60)
                                                                  CAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATG                             >  SRR4302534.1037470/1‑94 (MQ=60)
                                                                  CAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATG                             >  SRR4302534.3497941/1‑94 (MQ=60)
                                                                  CAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATG                             >  SRR4302534.2400474/1‑94 (MQ=60)
                                                                  CAAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATG                             <  SRR4302534.4178746/94‑1 (MQ=60)
                                                                   AAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGC                            <  SRR4302534.2995362/94‑1 (MQ=60)
                                                                   AAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGC                            <  SRR4302534.578219/94‑1 (MQ=60)
                                                                   AAAGCGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGC                            >  SRR4302534.5751244/1‑94 (MQ=60)
                                                                       CGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTG                        >  SRR4302534.5778137/1‑94 (MQ=60)
                                                                       CGCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTG                        >  SRR4302534.3756429/1‑94 (MQ=60)
                                                                        GCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGC                       >  SRR4302534.1394072/1‑94 (MQ=60)
                                                                        GCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGC                       >  SRR4302534.5760042/1‑94 (MQ=60)
                                                                        GCACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGA                       >  SRR4302534.4216515/1‑94 (MQ=60)
                                                                         CACTGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCA                      >  SRR4302534.3525939/1‑94 (MQ=60)
                                                                            TGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACG                   <  SRR4302534.1911258/94‑1 (MQ=60)
                                                                            TGTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACG                   >  SRR4302534.2137365/1‑94 (MQ=60)
                                                                             TTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGC                  >  SRR4302534.2617609/1‑94 (MQ=60)
                                                                             GTTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGC                  >  SRR4302534.2974410/1‑94 (MQ=60)
                                                                              TTCCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCC                 <  SRR4302534.4902397/94‑1 (MQ=60)
                                                                                CCGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTT               <  SRR4302534.2936140/94‑1 (MQ=60)
                                                                                 CGGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTT              <  SRR4302534.5497237/94‑1 (MQ=60)
                                                                                  GGCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTG             >  SRR4302534.306323/1‑94 (MQ=60)
                                                                                   GCGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGT            <  SRR4302534.371641/94‑1 (MQ=60)
                                                                                   GCGTTGAGAAGCGCCGGAAAACGATTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTAGACATCCATGCACTGCCACGCCTTTGT            >  SRR4302534.1081886/1‑94 (MQ=60)
                                                                                    CGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGGTGCCGTAGGCGATGCGGCTGCACTTTCTCGCCAGCCATGCAGTGTAACGCCTTTGTC           >  SRR4302534.4342345/1‑94 (MQ=60)
                                                                                    CGTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTC           >  SRR4302534.3196340/1‑94 (MQ=60)
                                                                                     GTTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCG          <  SRR4302534.6556384/94‑1 (MQ=60)
                                                                                      TTGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGG         <  SRR4302534.915237/94‑1 (MQ=60)
                                                                                       TGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGG        <  SRR4302534.6398796/94‑1 (MQ=60)
                                                                                       TGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGG        >  SRR4302534.3019377/1‑94 (MQ=60)
                                                                                       TGAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGG        >  SRR4302534.1072160/1‑94 (MQ=60)
                                                                                        GAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGC       >  SRR4302534.1931184/1‑94 (MQ=60)
                                                                                        GAGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGC       <  SRR4302534.2413797/94‑1 (MQ=60)
                                                                                         AGAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCC      >  SRR4302534.2695677/1‑94 (MQ=60)
                                                                                         AGAAGCGCCGGAAAACGTTTCAAACACCCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATTCATGCACTGCAACGCCTCTGTCGGGCG      >  SRR4302534.4357801/1‑94 (MQ=60)
                                                                                          GAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAG     >  SRR4302534.1088603/1‑94 (MQ=60)
                                                                                          GAAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAG     <  SRR4302534.6556431/94‑1 (MQ=60)
                                                                                          GAAGCCCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAG     <  SRR4302534.2692526/94‑1 (MQ=60)
                                                                                           AAGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGG    >  SRR4302534.4608011/1‑94 (MQ=60)
                                                                                            AGCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGC   >  SRR4302534.2005839/1‑94 (MQ=60)
                                                                                             GCGCCGGAAAACGTTTCAAACGGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCT  <  SRR4302534.249148/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCT  >  NC_000913/3738630‑3738816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑