Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,771,793	T→C	V629A (GTG→GCG)	mtlA →	PTS system, mannitol‑specific enzyme IIABC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,771,793	0	T	C	99.7%	1006.4 / ‑5.1	286	V629A (GTG→GCG)	mtlA	PTS system, mannitol‑specific enzyme IIABC components
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (141/144); minor base A (1/0); total (142/144)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 294 total aligned reads displayed.

AGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTAT  >  NC_000913/3771703‑3771882

 gCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCt                                                                                        <  1:5127679/93‑1 (MQ=255)

 gCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCt                                                                                        >  2:1198465/1‑93 (MQ=255)

 gCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCt                                                                                        >  2:5213630/1‑93 (MQ=255)

   acaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTgg                                                                                      >  1:5997644/1‑93 (MQ=255)

   acaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTgg                                                                                      >  2:6208847/1‑93 (MQ=255)

   acaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTgg                                                                                      >  1:6187032/1‑93 (MQ=255)

     aTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGaa                                                                                    >  2:2282569/1‑93 (MQ=255)

      ttCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAAc                                                                                   <  1:406710/93‑1 (MQ=255)

      ttCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAAc                                                                                   <  1:6376960/93‑1 (MQ=255)

         aGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAActgc                                                                                >  1:1803493/1‑93 (MQ=255)

         aGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAActgc                                                                                <  2:3477239/93‑1 (MQ=255)

          ggTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAActgct                                                                               >  2:1802428/1‑93 (MQ=255)

            ttATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTgg                                                                             <  2:2559714/93‑1 (MQ=255)

             tATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGc                                                                            >  2:3155625/1‑93 (MQ=255)

              aTCACAAGCATGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCa                                                                           <  1:3470590/93‑1 (MQ=255)

               tCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAg                                                                          >  1:2225529/1‑93 (MQ=255)

                 aCCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAggc                                                                        >  2:4629817/1‑92 (MQ=255)

                 aCCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGt                                                                        <  1:4175157/93‑1 (MQ=255)

                 aCCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGt                                                                        <  1:2808234/93‑1 (MQ=255)

                  ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTc                                                                       >  2:5531121/1‑93 (MQ=255)

                   cAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCg                                                                      >  1:3570070/1‑93 (MQ=255)

                    aGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCgt                                                                     >  1:1413946/1‑93 (MQ=255)

                     gCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCgta                                                                    >  1:3111697/1‑93 (MQ=255)

                      ccTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCgtaa                                                                   <  1:3283501/93‑1 (MQ=255)

                        tGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCgtaagt                                                                 >  2:1497666/1‑93 (MQ=255)

                        tGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCgtaagt                                                                 >  2:1241539/1‑93 (MQ=255)

                         gACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCgtaagta                                                                >  1:3778497/1‑93 (MQ=255)

                         gACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCgtaagta                                                                >  2:5591274/1‑93 (MQ=255)

                           ccAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaat                                                              >  1:2448952/1‑93 (MQ=255)

                            cAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaatc                                                             >  1:5707048/1‑93 (MQ=255)

                            cAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGAGCTGGAACTGCTGGCAGGTCGTAAGTaatc                                                             >  1:1021611/1‑93 (MQ=255)

                             aaTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaatcc                                                            >  1:4251445/1‑93 (MQ=255)

                             aaTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaatcc                                                            >  2:3977400/1‑93 (MQ=255)

                             aaTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaatcc                                                            <  2:809370/93‑1 (MQ=255)

                              aTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaatcca                                                           >  2:2969844/1‑93 (MQ=255)

                              aTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaatcca                                                           >  1:5295413/1‑93 (MQ=255)

                               tGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaatccaa                                                          <  1:110559/93‑1 (MQ=255)

                                 cACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaatccaatc                                                        >  1:832274/1‑93 (MQ=255)

                                  aCTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTaatccaatcc                                                       <  2:4779471/93‑1 (MQ=255)

                                   cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCGCCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATccc                                                      <  2:482335/93‑1 (MQ=255)

                                   cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATccc                                                      >  2:3724339/1‑93 (MQ=255)

                                   cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATccc                                                      <  2:4663992/93‑1 (MQ=255)

                                     ggATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCAc                                                    >  1:2554530/1‑93 (MQ=255)

                                       atgatgAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCAccc                                                  >  2:4988234/1‑93 (MQ=255)

                                           tgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCtctc                                              >  2:2914038/1‑93 (MQ=255)

                                           tgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCtctc                                              >  1:2149386/1‑93 (MQ=255)

                                             aGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCca                                            >  2:3791801/1‑93 (MQ=255)

                                              gTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCcac                                           <  2:4807732/93‑1 (MQ=255)

                                              gTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCcac                                           >  2:1121341/1‑93 (MQ=255)

                                                 cGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATg                                        <  2:712303/93‑1 (MQ=255)

                                                  gTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATgg                                       <  2:1047142/93‑1 (MQ=255)

                                                  gTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATgg                                       >  2:3672927/1‑93 (MQ=255)

                                                  gTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATgg                                       >  1:4644878/1‑93 (MQ=255)

                                                   tCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGga                                      >  2:426957/1‑93 (MQ=255)

                                                   tCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGga                                      >  2:3117163/1‑93 (MQ=255)

                                                   tCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGga                                      >  2:3246320/1‑93 (MQ=255)

                                                   tCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGga                                      >  2:887398/1‑93 (MQ=255)

                                                   tCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGga                                      <  2:1327425/93‑1 (MQ=255)

                                                     aTCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGgaga                                    <  2:541743/93‑1 (MQ=255)

                                                     aTCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGgaga                                    <  2:3077471/93‑1 (MQ=255)

                                                      tCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGaa                                   <  1:2399976/93‑1 (MQ=255)

                                                        gAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAgg                                 <  1:5917909/93‑1 (MQ=255)

                                                         aGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAggg                                >  2:1280650/1‑92 (MQ=255)

                                                         aGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAggg                                >  2:38388/1‑92 (MQ=255)

                                                         aGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                <  2:590759/93‑1 (MQ=255)

                                                         aGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                <  2:3003350/93‑1 (MQ=255)

                                                          gCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                               <  1:6120946/93‑1 (MQ=255)

                                                          gCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                               <  2:1416254/93‑1 (MQ=255)

                                                          gCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                               >  2:3288170/1‑93 (MQ=255)

                                                            gTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTggg                             <  1:872384/93‑1 (MQ=255)

                                                            gTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTggg                             >  1:932921/1‑93 (MQ=255)

                                                             tCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgggg                            <  1:2980486/93‑1 (MQ=255)

                                                             tCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCCCTCCACATGGAGAAGGTgggg                            >  1:745173/1‑93 (MQ=255)

                                                             gcTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgggg                            <  2:3353168/92‑1 (MQ=255)

                                                               tGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGtt                          <  1:4121881/93‑1 (MQ=255)

                                                                ggCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTa                         <  2:1561045/93‑1 (MQ=255)

                                                                ggCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTa                         >  2:5954858/1‑93 (MQ=255)

                                                                  cacacaCCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAt                       <  2:2121469/93‑1 (MQ=255)

                                                                   ccacacCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAtt                      <  1:160764/92‑1 (MQ=255)

                                                                   acacacCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAtt                      >  2:1920799/1‑93 (MQ=255)

                                                                    cacacCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTg                     >  1:171523/1‑93 (MQ=255)

                                                                     acacCACCAGCGTGGATGAAGCACTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCGCCCTCTCCACATGGAGAAGGTGGGGTTAATTGc                    <  2:2767760/93‑1 (MQ=255)

                                                                       accaccAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCt                  <  2:4494578/93‑1 (MQ=255)

                                                                          accaGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGAt               <  1:3959277/93‑1 (MQ=255)

                                                                          accaGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGAt               <  1:2037663/93‑1 (MQ=255)

                                                                           ccaGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATg              >  1:122324/1‑93 (MQ=255)

                                                                           ccaGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATg              >  1:5413635/1‑93 (MQ=255)

                                                                            caGGGTGGGTGAAGCGGTGGAACTGCTGGGAGGGCGTAAGTAATCCAATCCCCCCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgc             <  1:275495/93‑1 (MQ=255)

                                                                            caGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgc             <  1:3027343/93‑1 (MQ=255)

                                                                            caGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgc             <  2:1450987/93‑1 (MQ=255)

                                                                             aGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcg            <  1:2896734/93‑1 (MQ=255)

                                                                              gCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcgc           >  2:6422052/1‑93 (MQ=255)

                                                                                   gATGAAGCGCTGGAGCTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGc      <  1:5060702/93‑1 (MQ=255)

                                                                                   gATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGc      >  1:4419881/1‑93 (MQ=255)

                                                                                   gATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGc      <  1:4840058/93‑1 (MQ=255)

                                                                                   gATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGc      <  1:2657223/93‑1 (MQ=255)

                                                                                    aTGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt     <  2:5083670/93‑1 (MQ=255)

                                                                                    aTGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt     >  2:4104308/1‑93 (MQ=255)

                                                                                     tGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCtt    <  2:6355763/93‑1 (MQ=255)

                                                                                      gAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTa   >  1:4311158/1‑93 (MQ=255)

AGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTAT  >  NC_000913/3771703‑3771882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGG  >  NC_000913/3771700‑3771886

ACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGC                                                                                               >  SRR4302534.6043778/1‑94 (MQ=60)

 CGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGCTGAAGCG                                                                                              >  SRR4302534.5708497/1‑94 (MQ=60)

 CGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCG                                                                                              <  SRR4302534.3685471/94‑1 (MQ=60)

    GCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTG                                                                                           <  SRR4302534.5266117/94‑1 (MQ=60)

    GCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTG                                                                                           <  SRR4302534.5282529/94‑1 (MQ=60)

     CACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGG                                                                                          >  SRR4302534.6355421/1‑94 (MQ=60)

     CACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGG                                                                                          >  SRR4302534.6160580/1‑94 (MQ=60)

      ACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGA                                                                                         >  SRR4302534.5138779/1‑94 (MQ=60)

        ATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAAC                                                                                       <  SRR4302534.5671110/94‑1 (MQ=60)

         TTCAGGTTATCACCAGCGTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACTCCACCAGCGTGGATGAAGCGCTGGAACT                                                                                      <  SRR4302534.3401340/94‑1 (MQ=60)

         TTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACT                                                                                      <  SRR4302534.417106/94‑1 (MQ=60)

         TTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACT                                                                                      <  SRR4302534.6549198/94‑1 (MQ=60)

           CAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGC                                                                                    >  SRR4302534.1850332/1‑94 (MQ=60)

           CAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGC                                                                                    >  SRR4302534.33984/1‑94 (MQ=60)

           CAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGC                                                                                    >  SRR4302534.1372736/1‑94 (MQ=60)

           CAGGTTATCACCAGCCTGAACAATGCACTGGATGATGAGTCCGTCATCCAGCGTCTGGCACACAACACCAGCGTGGATGAAGAGCTCGAACAGC                                                                                    >  SRR4302534.2468795/1‑94 (MQ=60)

             GGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTG                                                                                  >  SRR4302534.1057224/1‑94 (MQ=60)

              GTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGG                                                                                 <  SRR4302534.4117037/94‑1 (MQ=60)

               TTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGC                                                                                <  SRR4302534.3318921/94‑1 (MQ=60)

               TTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGC                                                                                <  SRR4302534.1229609/94‑1 (MQ=60)

                 ATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAG                                                                              <  SRR4302534.6595515/94‑1 (MQ=60)

                 ATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAG                                                                              >  SRR4302534.2284474/1‑94 (MQ=60)

                 ATCACAAGCATGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAG                                                                              <  SRR4302534.3563870/94‑1 (MQ=60)

                  TCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGG                                                                             <  SRR4302534.5509230/94‑1 (MQ=60)

                    CCCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTC                                                                           <  SRR4302534.2624562/94‑1 (MQ=60)

                    ACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTC                                                                           <  SRR4302534.4287935/94‑1 (MQ=60)

                    ACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTC                                                                           <  SRR4302534.2882077/94‑1 (MQ=60)

                    ACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTC                                                                           >  SRR4302534.2238221/1‑94 (MQ=60)

                    ACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTC                                                                           <  SRR4302534.1792272/94‑1 (MQ=60)

                    ACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTC                                                                           <  SRR4302534.2833991/94‑1 (MQ=60)

                     CCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCG                                                                          >  SRR4302534.3666093/1‑94 (MQ=60)

                      CAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGT                                                                         >  SRR4302534.1451143/1‑94 (MQ=60)

                       AGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTA                                                                        >  SRR4302534.3193530/1‑94 (MQ=60)

                       AGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTA                                                                        <  SRR4302534.1018980/94‑1 (MQ=60)

                       AGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTA                                                                        <  SRR4302534.3108740/94‑1 (MQ=60)

                         CCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAG                                                                      <  SRR4302534.3371018/94‑1 (MQ=60)

                         CCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAG                                                                      <  SRR4302534.4235872/94‑1 (MQ=60)

                         CCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAG                                                                      <  SRR4302534.574860/94‑1 (MQ=60)

                          CTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGT                                                                     >  SRR4302534.4231972/1‑94 (MQ=60)

                           TGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTA                                                                    >  SRR4302534.3879940/1‑94 (MQ=60)

                           TGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTA                                                                    >  SRR4302534.1577838/1‑94 (MQ=60)

                           TGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTA                                                                    >  SRR4302534.3048772/1‑94 (MQ=60)

                           TGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTA                                                                    <  SRR4302534.2971062/94‑1 (MQ=60)

                            GACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAA                                                                   <  SRR4302534.2824191/94‑1 (MQ=60)

                            GACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAA                                                                   <  SRR4302534.1856300/94‑1 (MQ=60)

                             ACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAAT                                                                  <  SRR4302534.3827160/94‑1 (MQ=60)

                             ACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAAT                                                                  >  SRR4302534.2514030/1‑94 (MQ=60)

                             ACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAAT                                                                  >  SRR4302534.3887593/1‑94 (MQ=60)

                             ACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAAT                                                                  >  SRR4302534.918707/1‑94 (MQ=60)

                              CCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATC                                                                 >  SRR4302534.5861648/1‑94 (MQ=60)

                              CCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGAGCTGGAACTGCTGGCAGGTCGTAAGTAATC                                                                 >  SRR4302534.1047922/1‑94 (MQ=60)

                               CAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCC                                                                >  SRR4302534.4644975/1‑94 (MQ=60)

                               CAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCC                                                                >  SRR4302534.4366139/1‑94 (MQ=60)

                                AATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCA                                                               <  SRR4302534.2523883/94‑1 (MQ=60)

                                AATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCA                                                               >  SRR4302534.3598313/1‑94 (MQ=60)

                                AATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCA                                                               >  SRR4302534.5438336/1‑94 (MQ=60)

                                 ATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAA                                                              >  SRR4302534.1331014/1‑94 (MQ=60)

                                  TGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAAT                                                             <  SRR4302534.113507/94‑1 (MQ=60)

                                   GCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATC                                                            >  SRR4302534.193273/1‑94 (MQ=60)

                                   GCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATC                                                            >  SRR4302534.853203/1‑94 (MQ=60)

                                    CACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCC                                                           >  SRR4302534.4095865/1‑94 (MQ=60)

                                     ACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCC                                                          <  SRR4302534.433708/94‑1 (MQ=60)

                                      CTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCA                                                         <  SRR4302534.2473981/94‑1 (MQ=60)

                                       TGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCAC                                                        >  SRR4302534.2622241/1‑94 (MQ=60)

                                        GGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACC                                                       <  SRR4302534.4385823/94‑1 (MQ=60)

                                        GGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGCCGTAAGTAATCCAATCCCACC                                                       <  SRR4302534.6388945/94‑1 (MQ=60)

                                         GATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCC                                                      <  SRR4302534.4945520/94‑1 (MQ=60)

                                          ATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCT                                                     <  SRR4302534.881544/94‑1 (MQ=60)

                                           TGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTC                                                    <  SRR4302534.870346/94‑1 (MQ=60)

                                            gaagagccctaCATCGAGCGTCTGCCACACACCCCCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCT                                                   <  SRR4302534.4557730/83‑1 (MQ=60)

                                            GATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCT                                                   <  SRR4302534.3095940/94‑1 (MQ=60)

                                             ATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTC                                                  >  SRR4302534.2099349/1‑94 (MQ=60)

                                             ATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTC                                                  >  SRR4302534.2206110/1‑94 (MQ=60)

                                             ATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTC                                                  >  SRR4302534.639832/1‑94 (MQ=60)

                                              TGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCC                                                 <  SRR4302534.5897521/94‑1 (MQ=60)

                                              TGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGGCGTAAGTAATCCAATCCCACCCTCTCC                                                 >  SRR4302534.6134454/1‑94 (MQ=60)

                                               GAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCA                                                <  SRR4302534.4225279/94‑1 (MQ=60)

                                                 GTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACA                                              >  SRR4302534.2819863/1‑94 (MQ=60)

                                                    CGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGG                                           >  SRR4302534.6335885/1‑94 (MQ=60)

                                                    CGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGG                                           >  SRR4302534.4770960/1‑94 (MQ=60)

                                                     GTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGA                                          >  SRR4302534.2237856/1‑94 (MQ=60)

                                                      TCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAG                                         <  SRR4302534.2331448/94‑1 (MQ=60)

                                                      TCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAG                                         <  SRR4302534.619819/94‑1 (MQ=60)

                                                       CATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGA                                        >  SRR4302534.4331749/1‑94 (MQ=60)

                                                       CATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGA                                        <  SRR4302534.1736152/94‑1 (MQ=60)

                                                        ATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAA                                       <  SRR4302534.1499354/94‑1 (MQ=60)

                                                        ATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAA                                       >  SRR4302534.5919763/1‑94 (MQ=60)

                                                         TCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAG                                      <  SRR4302534.2463745/94‑1 (MQ=60)

                                                         TCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAG                                      <  SRR4302534.4800862/94‑1 (MQ=60)

                                                          TGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGG                                     >  SRR4302534.6128728/1‑94 (MQ=60)

                                                          CGAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGG                                     <  SRR4302534.5470940/94‑1 (MQ=60)

                                                           GAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGT                                    >  SRR4302534.2631451/1‑94 (MQ=60)

                                                           GAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGT                                    >  SRR4302534.4276013/1‑94 (MQ=60)

                                                           GAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGT                                    <  SRR4302534.4496891/94‑1 (MQ=60)

                                                           GAGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGT                                    <  SRR4302534.6078595/94‑1 (MQ=60)

                                                           GAGCGTCTGGCACACACCACCAGAGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGT                                    >  SRR4302534.5379476/1‑94 (MQ=60)

                                                            AGCGTCTGGCACACCACACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCATTCCCACCCTCTCCACATGGAGAAGGTG                                   <  SRR4302534.5108706/94‑1 (MQ=60)

                                                            AGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTG                                   >  SRR4302534.1734070/1‑94 (MQ=60)

                                                            AGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTG                                   <  SRR4302534.6319085/94‑1 (MQ=60)

                                                            AGCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTG                                   <  SRR4302534.925108/94‑1 (MQ=60)

                                                             GCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGG                                  <  SRR4302534.2574928/94‑1 (MQ=60)

                                                             GCGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGG                                  <  SRR4302534.6287436/94‑1 (MQ=60)

                                                              CGTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGG                                 >  SRR4302534.956653/1‑94 (MQ=60)

                                                               GTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGG                                <  SRR4302534.894425/94‑1 (MQ=60)

                                                               GTCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCCCTCCACATGGAGAAGGTGGGG                                >  SRR4302534.763643/1‑94 (MQ=60)

                                                                TCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGT                               <  SRR4302534.5236563/94‑1 (MQ=60)

                                                                TCTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGT                               <  SRR4302534.3058477/94‑1 (MQ=60)

                                                                 CTGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGGT                              >  SRR4302534.2874421/1‑94 (MQ=60)

                                                                  TGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTA                             <  SRR4302534.212710/94‑1 (MQ=60)

                                                                  TGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTA                             <  SRR4302534.4232821/94‑1 (MQ=60)

                                                                  TGGCACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTA                             <  SRR4302534.447899/94‑1 (MQ=60)

                                                                      CCACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTG                         <  SRR4302534.164977/94‑1 (MQ=60)

                                                                      ACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTG                         >  SRR4302534.176049/1‑94 (MQ=60)

                                                                      ACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTG                         <  SRR4302534.823332/94‑1 (MQ=60)

                                                                      ACACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTG                         <  SRR4302534.4031421/94‑1 (MQ=60)

                                                                       CACACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGC                        <  SRR4302534.6121287/94‑1 (MQ=60)

                                                                         CACCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCT                      <  SRR4302534.1787186/94‑1 (MQ=60)

                                                                           CCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGA                    >  SRR4302534.3165652/1‑94 (MQ=60)

                                                                           CCACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGACTGA                    >  SRR4302534.2407442/1‑94 (MQ=60)

                                                                             ACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCCATCCCACACTCTCCACATGGAGAAGGTGGGGATCATTGCCTGATG                  >  SRR4302534.4373341/1‑94 (MQ=60)

                                                                             ACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATG                  >  SRR4302534.5559253/1‑94 (MQ=60)

                                                                             ACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATG                  >  SRR4302534.125560/1‑94 (MQ=60)

                                                                             ACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATG                  <  SRR4302534.2090948/94‑1 (MQ=60)

                                                                             ACCAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATG                  <  SRR4302534.4065289/94‑1 (MQ=60)

                                                                               CAGGGTGGGTGAAGCGGTGGAACTGCTGGGAGGGCGTAAGTAATCCAATCCCCCCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCG                <  SRR4302534.282634/94‑1 (MQ=60)

                                                                               CAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCG                >  SRR4302534.3058817/1‑94 (MQ=60)

                                                                               CAGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCG                <  SRR4302534.3106698/94‑1 (MQ=60)

                                                                                AGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGC               >  SRR4302534.6270483/1‑94 (MQ=60)

                                                                                AGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGC               >  SRR4302534.1162390/1‑94 (MQ=60)

                                                                                AGCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGC               <  SRR4302534.2972771/94‑1 (MQ=60)

                                                                                 GCGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCT              <  SRR4302534.2966562/94‑1 (MQ=60)

                                                                                  CGTGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTA             >  SRR4302534.4700992/1‑94 (MQ=60)

                                                                                    TGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACG           >  SRR4302534.852908/1‑94 (MQ=60)

                                                                                    TGGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACG           >  SRR4302534.3357782/1‑94 (MQ=60)

                                                                                     TGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGC          >  SRR4302534.4539208/1‑94 (MQ=60)

                                                                                     GGATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGC          >  SRR4302534.666032/1‑94 (MQ=60)

                                                                                      GATGAAGCGCTGGAGCTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCT         <  SRR4302534.5197372/94‑1 (MQ=60)

                                                                                      GATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCT         <  SRR4302534.2727473/94‑1 (MQ=60)

                                                                                      GATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCT         <  SRR4302534.3661406/94‑1 (MQ=60)

                                                                                      GATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCT         >  SRR4302534.3834850/1‑94 (MQ=60)

                                                                                      GATGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCT         <  SRR4302534.4971342/94‑1 (MQ=60)

                                                                                        TGAAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTA       >  SRR4302534.4427958/1‑94 (MQ=60)

                                                                                          AAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGACTGATGCGCTACGCTTATC     >  SRR4302534.2320016/1‑94 (MQ=60)

                                                                                          AAGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGGTAATTGCCTGATGCGCTACGCTTATC     >  SRR4302534.3776115/1‑94 (MQ=60)

                                                                                           AGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCA    >  SRR4302534.2748385/1‑94 (MQ=60)

                                                                                           AGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCA    <  SRR4302534.6294600/94‑1 (MQ=60)

                                                                                           AGCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCA    >  SRR4302534.6253655/1‑94 (MQ=60)

                                                                                            GCGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAG   <  SRR4302534.2876991/94‑1 (MQ=60)

                                                                                             CGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGG  >  SRR4302534.5339805/1‑94 (MQ=60)

ACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGG  >  NC_000913/3771700‑3771886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`