Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,783,479 A→G D198G (GAT→GGT)  yibO → putative 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,783,4790AG99.6% 777.1 / NA 227D198G (GAT→GGT) yibOputative 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (108/118);  total (108/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

AATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATG  >  NC_000913/3783389‑3783568
                                                                                          |                                                                                         
aaTTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTc                                                                                         <  1:4910546/93‑1 (MQ=255)
  ttCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTcgc                                                                                       <  2:4286816/93‑1 (MQ=255)
  ttCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTcgc                                                                                       <  2:1487959/93‑1 (MQ=255)
   tCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTcgcg                                                                                      >  1:848052/1‑93 (MQ=255)
     gaagaaAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTa                                                                                    >  2:6454259/1‑93 (MQ=255)
     gaagaaAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTa                                                                                    <  1:3441363/93‑1 (MQ=255)
     gaagaaAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTCGGGTCGCGTa                                                                                    >  1:6107848/1‑93 (MQ=255)
      aagaaAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAg                                                                                   <  2:2071894/93‑1 (MQ=255)
      aagaaAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAg                                                                                   >  2:1892550/1‑93 (MQ=255)
         aaaaaTTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGaaa                                                                                <  2:5049064/93‑1 (MQ=255)
           aaaTTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGaaaaa                                                                              <  2:2872190/93‑1 (MQ=255)
           aaaTTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGaaaaa                                                                              >  1:1863093/1‑93 (MQ=255)
            aaTTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAg                                                                             >  1:1097834/1‑93 (MQ=255)
             aTTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGc                                                                            <  2:3371624/93‑1 (MQ=255)
             aTTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGc                                                                            >  1:2274847/1‑93 (MQ=255)
              tttGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCt                                                                           <  2:6407642/93‑1 (MQ=255)
              tttGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCt                                                                           <  2:5081366/93‑1 (MQ=255)
              tttGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCt                                                                           <  2:3055441/93‑1 (MQ=255)
                tGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAACAGCTTa                                                                         >  2:1122626/1‑93 (MQ=255)
                 gCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTAt                                                                        <  1:4430505/93‑1 (MQ=255)
                     cgcTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGAcc                                                                    <  1:6139908/93‑1 (MQ=255)
                     cgcTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGAcc                                                                    <  1:4658641/93‑1 (MQ=255)
                     cgcTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGAcc                                                                    <  1:316090/93‑1 (MQ=255)
                     cgcTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGAcc                                                                    >  1:6414675/1‑93 (MQ=255)
                      gcTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACct                                                                   <  2:4665580/93‑1 (MQ=255)
                      gcTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACct                                                                   >  1:3260108/1‑93 (MQ=255)
                        tGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTCACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACctgc                                                                 <  1:3075058/93‑1 (MQ=255)
                         gggCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACctgct                                                                >  2:2399873/1‑93 (MQ=255)
                           gCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGa                                                              >  1:3537215/1‑93 (MQ=255)
                           gCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGa                                                              <  2:2559831/93‑1 (MQ=255)
                           gCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGa                                                              >  1:4531804/1‑93 (MQ=255)
                            cAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGAc                                                             <  1:5824887/93‑1 (MQ=255)
                            cAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGAc                                                             <  1:4894624/93‑1 (MQ=255)
                             aaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGAct                                                            >  1:482310/1‑93 (MQ=255)
                             aaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGAct                                                            >  2:5951734/1‑93 (MQ=255)
                             aaaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGAct                                                            <  1:2959323/93‑1 (MQ=255)
                              aaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGActc                                                           >  2:5276402/1‑93 (MQ=255)
                              aaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGActc                                                           <  2:5630228/93‑1 (MQ=255)
                              aaGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGActc                                                           >  1:2403590/1‑93 (MQ=255)
                               aGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGActct                                                          <  2:2035338/93‑1 (MQ=255)
                               aGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGActct                                                          <  1:6454259/93‑1 (MQ=255)
                               aGGCCGCGTAGCGTCCATCAGTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGActct                                                          >  1:6214888/1‑93 (MQ=255)
                                ggCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTg                                                         <  1:5592238/93‑1 (MQ=255)
                                ggCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTg                                                         <  1:1745329/93‑1 (MQ=255)
                                  ccGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGgc                                                       <  1:4341239/93‑1 (MQ=255)
                                   cgcgTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGgcg                                                      >  2:4717710/1‑93 (MQ=255)
                                    gcgTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGgcgc                                                     <  2:4329383/93‑1 (MQ=255)
                                    gcgTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGgcgc                                                     <  2:322454/93‑1 (MQ=255)
                                      gTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTAt                                                                        >  1:3325339/1‑72 (MQ=255)
                                         gCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGTGGTCGCGTAGAAAAAGCTTATGACGTGCTGACTCTGGCGCAGGGc                                                >  2:3531998/1‑93 (MQ=255)
                                         gCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGc                                                >  1:4185619/1‑93 (MQ=255)
                                         gCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGAGGTCGCGTAGAAAAAGCTTATGACCTGCTGTCTCTGGCGCAGGGc                                                >  1:2578594/1‑93 (MQ=255)
                                           gTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGa                                              <  1:2357727/93‑1 (MQ=255)
                                             ccATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGt                                            >  1:3874852/1‑93 (MQ=255)
                                              catcatTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGtt                                           <  2:2614806/93‑1 (MQ=255)
                                               atcatTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTc                                          <  2:461888/93‑1 (MQ=255)
                                               atcatTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTc                                          <  2:3823724/93‑1 (MQ=255)
                                                 catTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCa                                        <  1:1977088/93‑1 (MQ=255)
                                                  atTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAg                                       <  1:2086039/93‑1 (MQ=255)
                                                  atTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAg                                       <  2:2842102/93‑1 (MQ=255)
                                                   ttGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAgc                                      >  2:5659343/1‑92 (MQ=255)
                                                     ggTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGcc                                    >  2:5730785/1‑93 (MQ=255)
                                                      gTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCg                                   >  1:5050367/1‑93 (MQ=255)
                                                      gTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCg                                   <  1:4153909/93‑1 (MQ=255)
                                                      gTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCg                                   >  1:6195214/1‑93 (MQ=255)
                                                       tCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGa                                  <  1:5360374/93‑1 (MQ=255)
                                                       tCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGa                                  >  2:1691932/1‑93 (MQ=255)
                                                        cGTTACTACGCGATGGACCGCGATAACCGTTGGGGACGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGCTCCAGGCCGAt                                 >  2:803273/1‑93 (MQ=255)
                                                               acGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATAccgccg                          >  2:1421433/1‑93 (MQ=255)
                                                               acGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATAccgccg                          <  1:5087474/93‑1 (MQ=255)
                                                               acGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATAccgccg                          <  1:744470/93‑1 (MQ=255)
                                                                cgcgATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGt                         <  2:4962768/93‑1 (MQ=255)
                                                                cgcgATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGt                         <  1:233419/93‑1 (MQ=255)
                                                                   gATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGc                      >  2:4823601/1‑93 (MQ=255)
                                                                   gATGGACCGCGATAACCGTTGGGGTCGCGAAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGc                      <  2:5482779/93‑1 (MQ=255)
                                                                    aTGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCt                     <  2:1515367/93‑1 (MQ=255)
                                                                       gACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGt                  <  2:5313634/93‑1 (MQ=255)
                                                                       gACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGt                  >  1:1033165/1‑93 (MQ=255)
                                                                       gACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCGGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGt                  <  2:6134782/93‑1 (MQ=255)
                                                                        aCCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGtt                 <  1:6015038/93‑1 (MQ=255)
                                                                         ccGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGttt                <  2:672525/93‑1 (MQ=255)
                                                                         ccGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGttt                >  1:1273918/1‑93 (MQ=255)
                                                                           gcgATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGc              >  1:5555663/1‑93 (MQ=255)
                                                                            cgATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCa             <  1:2266208/93‑1 (MQ=255)
                                                                             gATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAg            >  2:2803793/1‑93 (MQ=255)
                                                                             gATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAg            >  2:1988354/1‑93 (MQ=255)
                                                                               tAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGgc          <  1:6008367/93‑1 (MQ=255)
                                                                                aaCCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGgct         >  2:858161/1‑93 (MQ=255)
                                                                                aaCCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGgct         >  2:3736922/1‑93 (MQ=255)
                                                                                 aCCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGgctg        <  2:3971705/93‑1 (MQ=255)
                                                                                 aCCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGgctg        >  1:389240/1‑93 (MQ=255)
                                                                                   cGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGgctgct      <  1:166007/93‑1 (MQ=255)
                                                                                   cGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGgctgct      <  2:1002394/93‑1 (MQ=255)
                                                                                    gTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGAtt     >  1:2744327/1‑93 (MQ=255)
                                                                                     ttGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTa    <  2:5699113/93‑1 (MQ=255)
                                                                                       ggggTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATg  >  2:5490190/1‑93 (MQ=255)
                                                                                       ggggTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATg  <  2:5240048/93‑1 (MQ=255)
                                                                                       ggggTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATg  <  2:6197443/93‑1 (MQ=255)
                                                                                       ggggTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATg  >  1:2797474/1‑93 (MQ=255)
                                                                                       gggCTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATg  <  1:5658818/93‑1 (MQ=255)
                                                                                          |                                                                                         
AATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATG  >  NC_000913/3783389‑3783568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTC  >  NC_000913/3783386‑3783571
                                                                                             |                                                                                            
AAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGG                                                                                              <  SRR4302534.1965814/94‑1 (MQ=60)
AAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGG                                                                                              >  SRR4302534.136922/1‑94 (MQ=60)
 AAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGT                                                                                             >  SRR4302534.520509/1‑94 (MQ=60)
  AAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTC                                                                                            >  SRR4302534.2333472/1‑94 (MQ=60)
  AAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTC                                                                                            <  SRR4302534.2794222/94‑1 (MQ=60)
  AAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTC                                                                                            <  SRR4302534.5199962/94‑1 (MQ=60)
   AATTCGAAGAATAATTGGCCGCGCTGGGCAAAGGCCGCGGAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCG                                                                                           <  SRR4302534.283869/94‑1 (MQ=60)
   AATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCG                                                                                           <  SRR4302534.3542629/94‑1 (MQ=60)
   AATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCG                                                                                           <  SRR4302534.5043639/94‑1 (MQ=60)
     TTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCG                                                                                         >  SRR4302534.869426/1‑94 (MQ=60)
       CGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGTGGTCGCGTA                                                                                       >  SRR4302534.4571589/1‑94 (MQ=60)
       CGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTCGGGTCGCGTA                                                                                       >  SRR4302534.6273946/1‑94 (MQ=60)
        GAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAG                                                                                      <  SRR4302534.3533852/94‑1 (MQ=60)
        GAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAG                                                                                      >  SRR4302534.3228147/1‑94 (MQ=60)
        GAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAG                                                                                      >  SRR4302534.4362096/1‑94 (MQ=60)
         AAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGA                                                                                     <  SRR4302534.6357260/94‑1 (MQ=60)
          AGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAA                                                                                    >  SRR4302534.4773134/1‑94 (MQ=60)
            AAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAA                                                                                  >  SRR4302534.5045650/1‑94 (MQ=60)
            AAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAA                                                                                  >  SRR4302534.6372120/1‑94 (MQ=60)
             AAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAA                                                                                 >  SRR4302534.1911592/1‑94 (MQ=60)
              AAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAG                                                                                >  SRR4302534.1126412/1‑94 (MQ=60)
              AAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAG                                                                                >  SRR4302534.6225259/1‑94 (MQ=60)
               AATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGC                                                                               >  SRR4302534.2335198/1‑94 (MQ=60)
                ATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCT                                                                              >  SRR4302534.1888760/1‑94 (MQ=60)
                   TGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTAT                                                                           >  SRR4302534.3488814/1‑94 (MQ=60)
                   TGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTAT                                                                           <  SRR4302534.5194272/94‑1 (MQ=60)
                    GCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATG                                                                          <  SRR4302534.2962353/94‑1 (MQ=60)
                    GCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATG                                                                          <  SRR4302534.4550131/94‑1 (MQ=60)
                     CCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGA                                                                         <  SRR4302534.1329470/94‑1 (MQ=60)
                       GCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACC                                                                       >  SRR4302534.5416634/1‑94 (MQ=60)
                       GCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACC                                                                       >  SRR4302534.6587747/1‑94 (MQ=60)
                        CGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCT                                                                      <  SRR4302534.6560602/94‑1 (MQ=60)
                        CGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCT                                                                      <  SRR4302534.5713410/94‑1 (MQ=60)
                        CGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCT                                                                      >  SRR4302534.3346919/1‑94 (MQ=60)
                        CGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCT                                                                      <  SRR4302534.324229/94‑1 (MQ=60)
                        CGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCT                                                                      <  SRR4302534.4785136/94‑1 (MQ=60)
                        CGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCT                                                                      <  SRR4302534.6306906/94‑1 (MQ=60)
                         GCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTG                                                                     <  SRR4302534.5609013/94‑1 (MQ=60)
                          CTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGC                                                                    >  SRR4302534.2179351/1‑94 (MQ=60)
                           TGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCT                                                                   >  SRR4302534.1887609/1‑94 (MQ=60)
                           TGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTCACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCT                                                                   <  SRR4302534.3155834/94‑1 (MQ=60)
                            GGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTG                                                                  <  SRR4302534.5996483/94‑1 (MQ=60)
                            GGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTG                                                                  <  SRR4302534.2047957/94‑1 (MQ=60)
                             GGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGA                                                                 >  SRR4302534.3632363/1‑94 (MQ=60)
                             GGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGA                                                                 >  SRR4302534.4654608/1‑94 (MQ=60)
                               CAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACT                                                               <  SRR4302534.5027326/94‑1 (MQ=60)
                               CAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACT                                                               >  SRR4302534.494400/1‑94 (MQ=60)
                               CAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACT                                                               >  SRR4302534.2267392/1‑94 (MQ=60)
                               CAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACT                                                               <  SRR4302534.5982994/94‑1 (MQ=60)
                                AAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTC                                                              <  SRR4302534.3036868/94‑1 (MQ=60)
                                AAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTC                                                              >  SRR4302534.2582766/1‑94 (MQ=60)
                                AAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTC                                                              >  SRR4302534.2467472/1‑94 (MQ=60)
                                 AAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCT                                                             <  SRR4302534.1714884/94‑1 (MQ=60)
                                 AAGGCCGCGTAGCGTCCATCAGTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCT                                                             >  SRR4302534.6383752/1‑94 (MQ=60)
                                 AAGGCCGCGTAGCGTCCATCAGTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTCCTGACTCT                                                             >  SRR4302534.6383753/1‑94 (MQ=60)
                                  AGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTG                                                            <  SRR4302534.6628172/94‑1 (MQ=60)
                                   GGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGG                                                           >  SRR4302534.5042388/1‑94 (MQ=60)
                                   GGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGG                                                           <  SRR4302534.5743422/94‑1 (MQ=60)
                                   GGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGG                                                           <  SRR4302534.1790624/94‑1 (MQ=60)
                                    GCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGC                                                          <  SRR4302534.494760/94‑1 (MQ=60)
                                     CCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCG                                                         <  SRR4302534.4458530/94‑1 (MQ=60)
                                     CCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAAACGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCG                                                         >  SRR4302534.3965476/1‑94 (MQ=60)
                                        CGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGAatctatagatcggaagagc                                                      >  SRR4302534.3414273/1‑75 (MQ=60)
                                         GTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGG                                                     >  SRR4302534.6172099/1‑94 (MQ=60)
                                          TAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGG                                                    >  SRR4302534.3072196/1‑94 (MQ=60)
                                           AGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGC                                                   >  SRR4302534.4298740/1‑94 (MQ=60)
                                           AGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGAGGTCGCGTAGAAAAAGCTTATGACCTGCTGTCTCTGGCGCAGGGC                                                   >  SRR4302534.2646889/1‑94 (MQ=60)
                                             CGTCCATCATTGGGCGTTACTACGCGATGGACCGCGAGAACCGTTGGAGTCGCGTAGAAAAATCGTGTGTCCTGCTGACTCTGGCGCAGGGCGA                                                 >  SRR4302534.668628/1‑94 (MQ=60)
                                              GTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAG                                                <  SRR4302534.2420300/94‑1 (MQ=60)
                                               TCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGT                                               >  SRR4302534.3978884/1‑94 (MQ=60)
                                                CCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTT                                              >  SRR4302534.2836220/1‑94 (MQ=60)
                                                 CATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTC                                             >  SRR4302534.5826772/1‑94 (MQ=60)
                                                    CATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAG                                          <  SRR4302534.2028856/94‑1 (MQ=60)
                                                     ATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGG                                         <  SRR4302534.5194066/94‑1 (MQ=60)
                                                     ATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGG                                         <  SRR4302534.2140707/94‑1 (MQ=60)
                                                     ATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGG                                         >  SRR4302534.4763375/1‑94 (MQ=60)
                                                        GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCG                                      >  SRR4302534.5186737/1‑94 (MQ=60)
                                                        GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCG                                      <  SRR4302534.665892/94‑1 (MQ=60)
                                                        GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCG                                      <  SRR4302534.1121724/94‑1 (MQ=60)
                                                        GGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCG                                      >  SRR4302534.6363775/1‑94 (MQ=60)
                                                         GTCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGA                                     <  SRR4302534.4266054/94‑1 (MQ=60)
                                                          TCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTTGCGCAGGGCGAGTTCCAGGCCGAA                                    >  SRR4302534.3619137/1‑94 (MQ=60)
                                                          TCGTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGAT                                    <  SRR4302534.5504857/94‑1 (MQ=60)
                                                            GTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATAC                                  <  SRR4302534.988766/94‑1 (MQ=60)
                                                            GTTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATAC                                  <  SRR4302534.839003/94‑1 (MQ=60)
                                                             TTACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACC                                 <  SRR4302534.2438184/94‑1 (MQ=60)
                                                              TACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCG                                >  SRR4302534.491794/1‑94 (MQ=60)
                                                              TACTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCG                                >  SRR4302534.4978488/1‑94 (MQ=60)
                                                                CTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCC                              <  SRR4302534.4241692/94‑1 (MQ=60)
                                                                CTACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCC                              <  SRR4302534.5805965/94‑1 (MQ=60)
                                                                 TACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCG                             >  SRR4302534.4206909/1‑94 (MQ=60)
                                                                 TACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCG                             >  SRR4302534.4513680/1‑94 (MQ=60)
                                                                  ACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGT                            <  SRR4302534.762920/94‑1 (MQ=60)
                                                                  ACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGT                            >  SRR4302534.240864/1‑94 (MQ=60)
                                                                  ACGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGT                            <  SRR4302534.5224788/94‑1 (MQ=60)
                                                                   CGCGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTT                           <  SRR4302534.239527/94‑1 (MQ=60)
                                                                   CGCGAGGGACCGCAATAACCGTTGTGGTCGCTTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTT                           <  SRR4302534.2070124/94‑1 (MQ=60)
                                                                     CGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGC                         >  SRR4302534.6172254/1‑94 (MQ=60)
                                                                     CGATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGAGCGAGTTCCAGGCCGATACCGCCGTTGC                         >  SRR4302534.4354461/1‑94 (MQ=60)
                                                                      GATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCT                        <  SRR4302534.139074/94‑1 (MQ=60)
                                                                       ATGGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGGTCCAGGCCGATACCGCCGTTGCTG                       >  SRR4302534.3403792/1‑94 (MQ=60)
                                                                         GGACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGT                     >  SRR4302534.1059822/1‑94 (MQ=60)
                                                                          GACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTT                    >  SRR4302534.2615687/1‑94 (MQ=60)
                                                                           ACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTT                   <  SRR4302534.3320697/94‑1 (MQ=60)
                                                                           ACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTT                   <  SRR4302534.6592315/94‑1 (MQ=60)
                                                                           ACCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTT                   >  SRR4302534.1307455/1‑94 (MQ=60)
                                                                           ACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTT                   <  SRR4302534.6178478/94‑1 (MQ=60)
                                                                            CCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGGCGGTGCTGGTTTG                  >  SRR4302534.6303209/1‑94 (MQ=60)
                                                                            CCGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTG                  >  SRR4302534.2361696/1‑94 (MQ=60)
                                                                             CGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGC                 >  SRR4302534.2755642/1‑94 (MQ=60)
                                                                             CGCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGC                 >  SRR4302534.5705657/1‑94 (MQ=60)
                                                                              GCGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCA                >  SRR4302534.1931079/1‑94 (MQ=60)
                                                                               CGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAG               <  SRR4302534.2326305/94‑1 (MQ=60)
                                                                               CGATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAG               >  SRR4302534.3223564/1‑94 (MQ=60)
                                                                                GATAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGG              <  SRR4302534.5609198/94‑1 (MQ=60)
                                                                                  TAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCT            <  SRR4302534.6171610/94‑1 (MQ=60)
                                                                                  TAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCT            <  SRR4302534.3626143/94‑1 (MQ=60)
                                                                                  TAACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCT            >  SRR4302534.3994935/1‑94 (MQ=60)
                                                                                   AACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTG           >  SRR4302534.399186/1‑94 (MQ=60)
                                                                                    ACCGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGC          >  SRR4302534.1294464/1‑94 (MQ=60)
                                                                                      CGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTT        <  SRR4302534.170363/94‑1 (MQ=60)
                                                                                      CGTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGATT        >  SRR4302534.2816610/1‑94 (MQ=60)
                                                                                       GTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTA       >  SRR4302534.5981487/1‑94 (MQ=60)
                                                                                       GTTGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGACGCAGAGCGAGTTCCAGACCGATACCGCCGTTGCTGGTTTTCAGGCTGCTTA       >  SRR4302534.4288148/1‑94 (MQ=60)
                                                                                         TGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATG     <  SRR4302534.3378923/94‑1 (MQ=60)
                                                                                         TGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATG     >  SRR4302534.2340522/1‑94 (MQ=60)
                                                                                         TGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATG     >  SRR4302534.2871049/1‑94 (MQ=60)
                                                                                         TGGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATG     <  SRR4302534.75608/94‑1 (MQ=60)
                                                                                          GGGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGC    <  SRR4302534.467345/94‑1 (MQ=60)
                                                                                          GGGGTCGCGTAGAAAAAGCTTAAGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCAGCTTATGC    <  SRR4302534.6299942/94‑1 (MQ=60)
                                                                                          GGGCTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGC    <  SRR4302534.5812016/94‑1 (MQ=60)
                                                                                           GGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCT   <  SRR4302534.2077643/94‑1 (MQ=60)
                                                                                           GGGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCT   <  SRR4302534.4617806/94‑1 (MQ=60)
                                                                                            GGTCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTC  <  SRR4302534.3699830/94‑1 (MQ=60)
                                                                                             |                                                                                            
AAAAATTCGAAGAAAAATTTGCCGCGCTGGGCAAAGGCCGCGTAGCGTCCATCATTGGTCGTTACTACGCGATGGACCGCGATAACCGTTGGGATCGCGTAGAAAAAGCTTATGACCTGCTGACTCTGGCGCAGGGCGAGTTCCAGGCCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTC  >  NC_000913/3783386‑3783571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑