Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,788,398 A→G V192A (GTG→GCG)  tdh ← threonine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,788,3980AG99.6% 796.8 / ‑3.9 236coding (575/1026 nt)tdhthreonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (120/115);  minor base . (1/0);  total (121/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

Only 100 of 255 total aligned reads displayed.

TCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACC  >  NC_000913/3788308‑3788487
                                                                                          |                                                                                         
  aTTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATcgccac                                                                                       >  1:2891174/1‑93 (MQ=255)
  aTTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATcgccac                                                                                       <  2:342019/93‑1 (MQ=255)
   ttGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCcaca                                                                                      >  2:506624/1‑93 (MQ=255)
   ttGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCcaca                                                                                      >  1:6234446/1‑93 (MQ=255)
   ttGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCcaca                                                                                      >  1:1658876/1‑93 (MQ=255)
     gagaTTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAACGCGGTATTCGTTAACATCAGTGATCGCCACAtt                                                                                    >  2:5442520/1‑93 (MQ=255)
      agaTTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTg                                                                                   <  1:3033736/93‑1 (MQ=255)
       gaTTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGc                                                                                  >  2:712198/1‑93 (MQ=255)
         ttttCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGt                                                                                <  1:5727714/93‑1 (MQ=255)
         ttttCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGt                                                                                <  1:6403938/93‑1 (MQ=255)
         ttttCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGt                                                                                <  2:1381550/93‑1 (MQ=255)
         ttttCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGt                                                                                >  1:323298/1‑93 (MQ=255)
          tttCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTg                                                                               >  1:4883604/1‑93 (MQ=255)
          tttCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTg                                                                               <  1:1765989/93‑1 (MQ=255)
              tttGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCAcc                                                                           >  1:2239873/1‑93 (MQ=255)
               ttGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCa                                                                          >  2:4654717/1‑93 (MQ=255)
                tGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCaa                                                                         <  2:197095/93‑1 (MQ=255)
                 ggCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAAc                                                                        >  2:172892/1‑93 (MQ=255)
                     gctttAACCGCACGGGTTATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACgtgt                                                                    <  1:4323053/90‑1 (MQ=255)
                     aCGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACgtgt                                                                    <  2:4342577/93‑1 (MQ=255)
                     aCGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACgtgt                                                                    >  2:1087211/1‑93 (MQ=255)
                      cGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGtt                                                                   <  1:723907/93‑1 (MQ=255)
                       gTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGttt                                                                  >  1:3762612/1‑93 (MQ=255)
                       gTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGttt                                                                  >  2:2974890/1‑93 (MQ=255)
                        ttAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTc                                                                 <  1:5652771/93‑1 (MQ=255)
                        ttAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTc                                                                 <  1:5927132/93‑1 (MQ=255)
                        ttAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTc                                                                 <  1:5004406/93‑1 (MQ=255)
                        ttAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAACGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTc                                                                 >  1:1862088/1‑93 (MQ=255)
                        tgaaCCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTc                                                                 <  1:4046522/91‑1 (MQ=255)
                             cGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCAc                                                            >  1:4610522/1‑93 (MQ=255)
                             cGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCAc                                                            >  1:2273564/1‑93 (MQ=255)
                             cGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCAc                                                            <  1:6146019/93‑1 (MQ=255)
                             cGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGGATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCAc                                                            >  2:2740076/1‑93 (MQ=255)
                                aCGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACAAACGTGTTTCGCCAccgc                                                         >  2:2782265/1‑93 (MQ=255)
                                 cGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCAccgcc                                                        >  2:103147/1‑93 (MQ=255)
                                   ggTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTTACATCAGTGATCGCCACATTGCGTGAACCAACGTGTTTCGCCACcgccgc                                                      >  1:2088369/1‑93 (MQ=255)
                                   ggTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACcgccgc                                                      <  1:1604939/93‑1 (MQ=255)
                                   ggTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACcgccgc                                                      <  2:454866/93‑1 (MQ=255)
                                    gTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCt                                                     <  2:2929837/93‑1 (MQ=255)
                                    gTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCt                                                     >  2:760641/1‑93 (MQ=255)
                                    gTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCt                                                     <  2:126723/93‑1 (MQ=255)
                                    gTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCt                                                     <  2:2328626/93‑1 (MQ=255)
                                       aTACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGcc                                                  <  1:272799/93‑1 (MQ=255)
                                       aTACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGcc                                                  <  1:4703213/93‑1 (MQ=255)
                                        tACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCa                                                 >  2:160514/1‑93 (MQ=255)
                                         aCCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCat                                                <  1:5517116/93‑1 (MQ=255)
                                          cccATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCata                                               <  2:4943811/93‑1 (MQ=255)
                                          cccATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCata                                               >  2:3656049/1‑93 (MQ=255)
                                           ccATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCataa                                              >  1:3910010/1‑93 (MQ=255)
                                              ttttACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATAc                                           <  1:1002738/93‑1 (MQ=255)
                                                 tACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCaa                                        >  2:5931077/1‑93 (MQ=255)
                                                   cgcgCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATc                                      <  2:5126426/93‑1 (MQ=255)
                                                   cgcgCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATc                                      >  1:2385992/1‑93 (MQ=255)
                                                   cgcgCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATc                                      <  2:4781915/93‑1 (MQ=255)
                                                    gcgcCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCCCCGCCGCTGCCATAATACCAATCg                                     <  2:639008/93‑1 (MQ=255)
                                                    gcgcCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCg                                     >  2:336094/1‑93 (MQ=255)
                                                    gcgcCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCg                                     <  1:1216767/93‑1 (MQ=255)
                                                    gcgcCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCg                                     <  2:3048694/93‑1 (MQ=255)
                                                      gcCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCggg                                   <  2:4857003/93‑1 (MQ=255)
                                                       ccAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGc                                  >  1:4758331/1‑93 (MQ=255)
                                                        cAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGcc                                 <  2:248527/93‑1 (MQ=255)
                                                        cAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGcc                                 >  1:1554405/1‑93 (MQ=255)
                                                         aGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCt                                >  1:2703353/1‑93 (MQ=255)
                                                         aGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCt                                <  2:3211536/93‑1 (MQ=255)
                                                         aGCTCAAGGCGGTATTCGTTAACATCAGTGATC‑GCACATTGCGTGCACCCACGTGTTTCCCCAACGCCCCTGCCATAAAACCAATCCGGGCTg                               >  2:2223569/1‑93 (MQ=255)
                                                          gCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTg                               >  2:2070134/1‑93 (MQ=255)
                                                          gCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTg                               >  2:3477101/1‑93 (MQ=255)
                                                            tCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCa                             >  2:5471675/1‑93 (MQ=255)
                                                             cAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCAc                            <  2:1213990/93‑1 (MQ=255)
                                                             cAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCAc                            >  1:4381969/1‑93 (MQ=255)
                                                               aGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCa                          <  2:6310798/93‑1 (MQ=255)
                                                               aGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCa                          >  1:5144565/1‑93 (MQ=255)
                                                                 gCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGa                        >  1:104135/1‑93 (MQ=255)
                                                                 gCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGa                        <  1:5447339/93‑1 (MQ=255)
                                                                   ggTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGaaa                      >  2:1744448/1‑93 (MQ=255)
                                                                    gTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAAc                     >  1:5804618/1‑93 (MQ=255)
                                                                    gTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAAc                     <  2:5217590/93‑1 (MQ=255)
                                                                      aTTCGTTAGCATCAGTGATCGCCCCATGGCGTGCTCCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAACCGGGCCTGCACCAGAAACCa                   <  2:6152132/93‑1 (MQ=255)
                                                                      aTTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCa                   <  1:3278148/93‑1 (MQ=255)
                                                                       ttCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAg                  <  1:882265/93‑1 (MQ=255)
                                                                        tCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGc                 <  1:5273253/93‑1 (MQ=255)
                                                                          gTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGcac               >  2:1882106/1‑93 (MQ=255)
                                                                          gTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGcac               <  2:6043221/93‑1 (MQ=255)
                                                                          gTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGcac               >  2:5715500/1‑93 (MQ=255)
                                                                           ttAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGcaca              >  1:3634468/1‑93 (MQ=255)
                                                                             aaCATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACTGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATc            >  1:4954992/1‑93 (MQ=255)
                                                                             aaCATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATc            <  1:2658987/93‑1 (MQ=255)
                                                                              aCATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCt           <  1:2555879/93‑1 (MQ=255)
                                                                              aCATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCt           <  2:3285542/93‑1 (MQ=255)
                                                                               cATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCtt          >  2:4632979/1‑93 (MQ=255)
                                                                               cATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCtt          <  2:5618415/93‑1 (MQ=255)
                                                                                aTCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTc         <  1:4811931/93‑1 (MQ=255)
                                                                                   aGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGcc      >  2:1426386/1‑93 (MQ=255)
                                                                                    gTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGccc     <  1:939906/93‑1 (MQ=255)
                                                                                      gATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCccac   >  1:4829757/1‑93 (MQ=255)
                                                                                      gATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCccac   >  2:3846301/1‑93 (MQ=255)
                                                                                      gATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAACCGGGCCTGCACCAGAAACCAGCACATCTTCGCccac   <  1:1440042/93‑1 (MQ=255)
                                                                                       aTCTCCACACTCCGTGCGCCAACGTGTTCCGCCACCGCCTCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCccacc  <  1:127024/93‑1 (MQ=255)
                                                                                       aTCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCccacc  >  2:581876/1‑93 (MQ=255)
                                                                                       aTCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCccacc  >  2:4379926/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACC  >  NC_000913/3788308‑3788487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGAT  >  NC_000913/3788305‑3788491
                                                                                             |                                                                                             
ACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCG                                                                                               <  SRR4302534.2907543/94‑1 (MQ=60)
ACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCG                                                                                               >  SRR4302534.357106/1‑94 (MQ=60)
   TCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCA                                                                                            >  SRR4302534.154663/1‑94 (MQ=60)
    CATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCAC                                                                                           >  SRR4302534.991261/1‑94 (MQ=60)
    CATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCAC                                                                                           >  SRR4302534.2967042/1‑94 (MQ=60)
    CATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCAC                                                                                           >  SRR4302534.1901006/1‑94 (MQ=60)
     ATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACA                                                                                          >  SRR4302534.6403674/1‑94 (MQ=60)
     ATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACA                                                                                          <  SRR4302534.887169/94‑1 (MQ=60)
     ATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACA                                                                                          >  SRR4302534.1702091/1‑94 (MQ=60)
     ATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACA                                                                                          >  SRR4302534.3996335/1‑94 (MQ=60)
     ATTGAGATTTTCTTTGGCGACGTTAACCCCACGGGTGATACCAATTTTACGCGCCAGCTCAAGCCGGTATTCGTTAACATCAGTGATCGCCACA                                                                                          >  SRR4302534.1759546/1‑94 (MQ=60)
      TTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACAT                                                                                         <  SRR4302534.5650588/94‑1 (MQ=60)
       TGATATTTTCTTTGGCGACGTTAGCCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATT                                                                                        <  SRR4302534.4889357/94‑1 (MQ=60)
       TGAGATTTTGTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATT                                                                                        >  SRR4302534.6479795/1‑94 (MQ=60)
       TGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATT                                                                                        <  SRR4302534.312264/94‑1 (MQ=60)
       TGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATT                                                                                        >  SRR4302534.2346975/1‑94 (MQ=60)
       TGAGATGTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATT                                                                                        <  SRR4302534.937027/94‑1 (MQ=60)
        GAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTG                                                                                       <  SRR4302534.5353599/94‑1 (MQ=60)
         AGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGC                                                                                      >  SRR4302534.110117/1‑94 (MQ=60)
         AGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGC                                                                                      <  SRR4302534.3113288/94‑1 (MQ=60)
           ATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGT                                                                                    >  SRR4302534.331607/1‑94 (MQ=60)
           ATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGT                                                                                    >  SRR4302534.201020/1‑94 (MQ=60)
            TTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTG                                                                                   <  SRR4302534.6576824/94‑1 (MQ=60)
            TTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTG                                                                                   >  SRR4302534.5016049/1‑94 (MQ=60)
            TTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTG                                                                                   <  SRR4302534.5882908/94‑1 (MQ=60)
             TTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGC                                                                                  <  SRR4302534.1811851/94‑1 (MQ=60)
                CTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACC                                                                               >  SRR4302534.2299233/1‑94 (MQ=60)
                 TTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCA                                                                              <  SRR4302534.16783/94‑1 (MQ=60)
                   TGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAAC                                                                            <  SRR4302534.334885/94‑1 (MQ=60)
                      CGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTG                                                                         <  SRR4302534.671347/94‑1 (MQ=60)
                      CGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTG                                                                         >  SRR4302534.5328535/1‑94 (MQ=60)
                       GACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGT                                                                        >  SRR4302534.2779270/1‑94 (MQ=60)
                        gctTTAACCGCACGGGTTATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTT                                                                       <  SRR4302534.4440040/91‑1 (MQ=60)
                        ACGTTAGCCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGAGCGCCACATTGCGTGCACCAACGTGTT                                                                       <  SRR4302534.2357010/94‑1 (MQ=60)
                        ACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTT                                                                       >  SRR4302534.3070704/1‑94 (MQ=60)
                         CGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTT                                                                      <  SRR4302534.2316284/94‑1 (MQ=60)
                         CGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTT                                                                      <  SRR4302534.741781/94‑1 (MQ=60)
                         CGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTT                                                                      >  SRR4302534.3863635/1‑94 (MQ=60)
                          GTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTC                                                                     >  SRR4302534.4050360/1‑94 (MQ=60)
                          GTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTC                                                                     <  SRR4302534.4869991/94‑1 (MQ=60)
                          GTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTC                                                                     >  SRR4302534.4875150/1‑94 (MQ=60)
                          GTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGCTTC                                                                     >  SRR4302534.4811469/1‑94 (MQ=60)
                          GTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAACGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTC                                                                     >  SRR4302534.1910559/1‑94 (MQ=60)
                           TTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCG                                                                    <  SRR4302534.5805770/94‑1 (MQ=60)
                           TTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCG                                                                    <  SRR4302534.6088083/94‑1 (MQ=60)
                           TTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCG                                                                    <  SRR4302534.5139692/94‑1 (MQ=60)
                           TGAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCG                                                                    <  SRR4302534.4155057/94‑1 (MQ=60)
                            TAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGC                                                                   >  SRR4302534.2688634/1‑94 (MQ=60)
                            TAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGC                                                                   >  SRR4302534.4955900/1‑94 (MQ=60)
                             AACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCC                                                                  >  SRR4302534.3113275/1‑94 (MQ=60)
                             AACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCC                                                                  >  SRR4302534.567937/1‑94 (MQ=60)
                               CCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCAC                                                                >  SRR4302534.4735662/1‑94 (MQ=60)
                               CCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCAC                                                                >  SRR4302534.2333869/1‑94 (MQ=60)
                                CGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACC                                                               <  SRR4302534.6389392/94‑1 (MQ=60)
                                CGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACC                                                               <  SRR4302534.6313153/94‑1 (MQ=60)
                                CGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACC                                                               <  SRR4302534.6311218/94‑1 (MQ=60)
                                CGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACC                                                               >  SRR4302534.4002493/1‑94 (MQ=60)
                                     GGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTTACATCAGTGATCGCCACATTGCGTGAACCAACGTGTTTCGCCACCGCCGC                                                          >  SRR4302534.2143115/1‑94 (MQ=60)
                                     GGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGC                                                          >  SRR4302534.2030753/1‑94 (MQ=60)
                                      GGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCT                                                         <  SRR4302534.4269009/94‑1 (MQ=60)
                                      GGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCT                                                         <  SRR4302534.1646856/94‑1 (MQ=60)
                                      GGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCT                                                         >  SRR4302534.3282177/1‑94 (MQ=60)
                                        TGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGC                                                       <  SRR4302534.5012602/94‑1 (MQ=60)
                                         GATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCC                                                      <  SRR4302534.5972290/94‑1 (MQ=60)
                                          ATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCA                                                     <  SRR4302534.4830890/94‑1 (MQ=60)
                                          ATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCA                                                     <  SRR4302534.279864/94‑1 (MQ=60)
                                           TACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACTAACGTGTTTCGCCACCGCCGCTGCCAT                                                    >  SRR4302534.1031174/1‑94 (MQ=60)
                                            ACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATA                                                   <  SRR4302534.5665986/94‑1 (MQ=60)
                                            ACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATA                                                   <  SRR4302534.5071967/94‑1 (MQ=60)
                                             CCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAA                                                  >  SRR4302534.4014851/1‑94 (MQ=60)
                                             CCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAA                                                  >  SRR4302534.1712353/1‑94 (MQ=60)
                                              CCATTTTACGCGCCAGCTCAAGTCGTTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAAT                                                 <  SRR4302534.1629161/94‑1 (MQ=60)
                                              CCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAAT                                                 <  SRR4302534.3493902/94‑1 (MQ=60)
                                               CATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATA                                                >  SRR4302534.974672/1‑94 (MQ=60)
                                               CATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATA                                                <  SRR4302534.4920453/94‑1 (MQ=60)
                                                ATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATAC                                               <  SRR4302534.5541660/94‑1 (MQ=60)
                                                ATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATAC                                               >  SRR4302534.4691279/1‑94 (MQ=60)
                                                 TTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACC                                              <  SRR4302534.1028453/94‑1 (MQ=60)
                                                 TTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACC                                              <  SRR4302534.1264646/94‑1 (MQ=60)
                                                  TTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCA                                             >  SRR4302534.2753938/1‑94 (MQ=60)
                                                  TTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCA                                             <  SRR4302534.5127800/94‑1 (MQ=60)
                                                   TTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATAGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAA                                            >  SRR4302534.891231/1‑94 (MQ=60)
                                                     ACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATC                                          >  SRR4302534.2449392/1‑94 (MQ=60)
                                                       GCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGG                                        >  SRR4302534.1322984/1‑94 (MQ=60)
                                                       GCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGG                                        <  SRR4302534.1248792/94‑1 (MQ=60)
                                                         GCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGC                                      >  SRR4302534.4887538/1‑94 (MQ=60)
                                                          CCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCC                                     >  SRR4302534.1595111/1‑94 (MQ=60)
                                                           CAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCT                                    <  SRR4302534.1315887/94‑1 (MQ=60)
                                                           CAGCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCT                                    >  SRR4302534.2774758/1‑94 (MQ=60)
                                                             GCTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGC                                  <  SRR4302534.5093289/94‑1 (MQ=60)
                                                              CTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCGCCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCA                                 <  SRR4302534.4458660/94‑1 (MQ=60)
                                                              CTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCA                                 >  SRR4302534.2226123/1‑94 (MQ=60)
                                                              CTCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCA                                 <  SRR4302534.4315232/94‑1 (MQ=60)
                                                               TCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCAC                                >  SRR4302534.5808200/1‑94 (MQ=60)
                                                               TCAAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCAC                                >  SRR4302534.4500096/1‑94 (MQ=60)
                                                                 AAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCA                              >  SRR4302534.3776471/1‑94 (MQ=60)
                                                                 AAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCA                              >  SRR4302534.5283383/1‑94 (MQ=60)
                                                                 AAGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCA                              <  SRR4302534.2787811/94‑1 (MQ=60)
                                                                  AGGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAG                             >  SRR4302534.1128242/1‑94 (MQ=60)
                                                                   GGCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGA                            >  SRR4302534.106924/1‑94 (MQ=60)
                                                                    GCGGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAA                           <  SRR4302534.5594107/94‑1 (MQ=60)
                                                                      GGTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAAC                         >  SRR4302534.5962154/1‑94 (MQ=60)
                                                                       GTATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACC                        >  SRR4302534.3674676/1‑94 (MQ=60)
                                                                        TATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCA                       <  SRR4302534.2479619/94‑1 (MQ=60)
                                                                         ATTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAG                      <  SRR4302534.3365509/94‑1 (MQ=60)
                                                                          TTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGC                     <  SRR4302534.904580/94‑1 (MQ=60)
                                                                          TTCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGC                     <  SRR4302534.5953018/94‑1 (MQ=60)
                                                                           TCGTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCA                    <  SRR4302534.5415681/94‑1 (MQ=60)
                                                                             GTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACA                  <  SRR4302534.2834459/94‑1 (MQ=60)
                                                                             GTTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACA                  >  SRR4302534.3732165/1‑94 (MQ=60)
                                                                              TTAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACAT                 >  SRR4302534.578352/1‑94 (MQ=60)
                                                                              TTAACATCACTGATCACCCTATTGCGTGCACCAGCGTGTTTCGCGACCGCCGCTGCCATAAGACCAATCGGGCCTGCACCAGAAACCAGCATAT                 <  SRR4302534.5174718/94‑1 (MQ=60)
                                                                               TAACATCGGTGTTCACCCCATTGCGTGCGCCAACGTGTTTCGCAACCGCCGCTGCCATAATACAAATCGGGCCTGCACCAGAAACCAGCACATC                <  SRR4302534.4596847/94‑1 (MQ=60)
                                                                               TAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACTGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATC                >  SRR4302534.5089185/1‑94 (MQ=60)
                                                                               TAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAATCAGCACATC                >  SRR4302534.1462915/1‑94 (MQ=60)
                                                                               TAACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATC                >  SRR4302534.5651899/1‑94 (MQ=60)
                                                                                AACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCT               <  SRR4302534.1644471/94‑1 (MQ=60)
                                                                                AACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCT               <  SRR4302534.2729285/94‑1 (MQ=60)
                                                                                AACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCT               >  SRR4302534.652330/1‑94 (MQ=60)
                                                                                AACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCT               <  SRR4302534.5215441/94‑1 (MQ=60)
                                                                                 ACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTT              <  SRR4302534.2690409/94‑1 (MQ=60)
                                                                                 ACATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTT              <  SRR4302534.2623625/94‑1 (MQ=60)
                                                                                  CATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTC             >  SRR4302534.5949026/1‑94 (MQ=60)
                                                                                   ATCAGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCG            <  SRR4302534.4942568/94‑1 (MQ=60)
                                                                                      AGTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCC         >  SRR4302534.6152496/1‑94 (MQ=60)
                                                                                       GTGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCA        <  SRR4302534.963811/94‑1 (MQ=60)
                                                                                        TGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCAC       >  SRR4302534.2234610/1‑94 (MQ=60)
                                                                                        TGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCAC       >  SRR4302534.447813/1‑94 (MQ=60)
                                                                                        TGATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCAC       >  SRR4302534.4960803/1‑94 (MQ=60)
                                                                                         GATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACC      <  SRR4302534.4988980/94‑1 (MQ=60)
                                                                                         GATCGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAACCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACC      <  SRR4302534.1477946/94‑1 (MQ=60)
                                                                                          ATCTCCACACTCCGTGCGCCAACGTGTTCCGCCACCGCCTCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCA     <  SRR4302534.130387/94‑1 (MQ=60)
                                                                                            CGCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGA   <  SRR4302534.4489460/94‑1 (MQ=60)
                                                                                             GCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGAT  >  SRR4302534.2518831/1‑94 (MQ=60)
                                                                                             GCCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGAT  >  SRR4302534.5385932/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGAT  >  NC_000913/3788305‑3788491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑