Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,899,440 T→C T137A (ACC→GCC)  yieC ← putative receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,899,4400TC99.1% 750.0 / ‑2.6 226T137A (ACC→GCC) yieCputative receptor protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (99/125);  total (101/125)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-02

Only 100 of 239 total aligned reads displayed.

TTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAAT  >  NC_000913/3899350‑3899529
                                                                                          |                                                                                         
  aaCTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCcccc                                                                                       <  1:854209/93‑1 (MQ=255)
  aaCTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCcccc                                                                                       <  1:3889034/93‑1 (MQ=255)
     tGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCaa                                                                                    >  1:549937/1‑93 (MQ=255)
      gCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAAc                                                                                   >  2:4074998/1‑93 (MQ=255)
       cAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAAcc                                                                                  <  2:4191882/93‑1 (MQ=255)
        aaaTCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCa                                                                                 <  1:3257016/93‑1 (MQ=255)
        aaaTCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCa                                                                                 >  1:2530241/1‑93 (MQ=255)
         aaTCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAg                                                                                <  1:1186889/93‑1 (MQ=255)
         aaTCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAg                                                                                >  1:4171820/1‑93 (MQ=255)
         aaTCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAg                                                                                <  2:4967812/93‑1 (MQ=255)
         aaTCAAACCAGCCGGAGGATTCGTTACCCAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGCGAACCATGAGAGGCGGCCCCCCAACCaa                                                                                >  2:336171/1‑92 (MQ=255)
          aTCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGa                                                                               <  1:480108/93‑1 (MQ=255)
           acAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGaa                                                                              <  1:5786595/92‑1 (MQ=255)
            cAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAAc                                                                             <  2:5173351/93‑1 (MQ=255)
            cAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAAc                                                                             <  2:5331230/93‑1 (MQ=255)
              aaCCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACgg                                                                           >  1:4327680/1‑93 (MQ=255)
              aaCCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACgg                                                                           >  2:4036644/1‑93 (MQ=255)
               aCCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGt                                                                          >  2:3096427/1‑93 (MQ=255)
                ccAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTa                                                                         >  2:5388004/1‑93 (MQ=255)
                  aGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAAt                                                                       >  2:1246324/1‑93 (MQ=255)
                   gCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATa                                                                      <  2:1030342/93‑1 (MQ=255)
                   gCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATa                                                                      <  2:2350042/93‑1 (MQ=255)
                    ccGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACAATGAGAGGCGGCCCCCCAACCAGAACGGTAATAg                                                                     <  1:5823786/93‑1 (MQ=255)
                     cGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCCGCACGGTAATAGc                                                                    >  1:4041729/1‑93 (MQ=255)
                     cGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGc                                                                    <  1:5942978/93‑1 (MQ=255)
                      ggAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGcc                                                                   <  1:2382962/93‑1 (MQ=255)
                      ggAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGcc                                                                   >  2:1483581/1‑93 (MQ=255)
                      ggAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGcc                                                                   <  2:5059105/93‑1 (MQ=255)
                        aGTGTTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGt                                                                 <  1:3953024/93‑1 (MQ=255)
                        aGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGt                                                                 <  1:2902467/93‑1 (MQ=255)
                        aGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGt                                                                 >  1:4364617/1‑93 (MQ=255)
                        aGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTAAGGGGAACAATGGGAGGCGGCCCCCAACCCAGAACGGAAAAAGCCGt                                                                 >  1:1777837/1‑93 (MQ=255)
                         gTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGtt                                                                <  1:2748100/93‑1 (MQ=255)
                         gTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGtt                                                                <  2:3898548/93‑1 (MQ=255)
                         gTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGtt                                                                <  1:1915617/93‑1 (MQ=255)
                          tATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTg                                                               >  2:763876/1‑93 (MQ=255)
                           aTTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGt                                                              >  1:6087682/1‑93 (MQ=255)
                               gTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACt                                                          >  1:2647247/1‑93 (MQ=255)
                               gTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACt                                                          <  1:197229/93‑1 (MQ=255)
                                ttACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTa                                                         >  1:2983431/1‑93 (MQ=255)
                                 tACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTaa                                                        <  1:4967431/93‑1 (MQ=255)
                                  aCCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTaaa                                                       <  2:5752973/93‑1 (MQ=255)
                                  aCCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTaaa                                                       >  2:4502392/1‑93 (MQ=255)
                                  aCCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTaaa                                                       >  2:1694907/1‑93 (MQ=255)
                                   ccAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAg                                                      >  1:3373915/1‑93 (MQ=255)
                                       aGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCaa                                                  <  1:1291101/93‑1 (MQ=255)
                                       aGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCaa                                                  >  2:4346631/1‑93 (MQ=255)
                                        gCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAt                                                 <  2:5091339/93‑1 (MQ=255)
                                        gCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAt                                                 >  2:3943394/1‑93 (MQ=255)
                                         cGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAtt                                                <  2:4572216/93‑1 (MQ=255)
                                         cGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAtt                                                <  1:635396/93‑1 (MQ=255)
                                          ggCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAttt                                               <  1:2484595/93‑1 (MQ=255)
                                           gCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTc                                              >  2:4778349/1‑93 (MQ=255)
                                           gCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAAATAAAGTCAATTTc                                              >  1:5216393/1‑93 (MQ=255)
                                             ccAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCAt                                            >  2:2253671/1‑93 (MQ=255)
                                               agagAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCt                                          >  2:2811417/1‑93 (MQ=255)
                                               agagAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCt                                          <  2:239320/93‑1 (MQ=255)
                                               agagAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCt                                          >  1:6262743/1‑93 (MQ=255)
                                               agagAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCt                                          <  2:901187/93‑1 (MQ=255)
                                                gagaACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCtt                                         >  2:356504/1‑93 (MQ=255)
                                                 agaACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGACGTTGTAACTAAAGCCAATTTTATCttt                                        >  2:3303534/1‑93 (MQ=255)
                                                  gaACGAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCGCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTc                                       <  1:3157525/93‑1 (MQ=255)
                                                  gaACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTc                                       <  1:119950/93‑1 (MQ=255)
                                                  gaACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTc                                       <  1:3511146/93‑1 (MQ=255)
                                                   aaCCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTca                                      >  1:1589090/1‑93 (MQ=255)
                                                      cAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACaa                                   >  1:2623957/1‑93 (MQ=255)
                                                       aaTCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACcaa                                  >  2:1102631/1‑93 (MQ=255)
                                                        aTCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAAt                                 <  2:1926326/93‑1 (MQ=255)
                                                          cGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAAttt                               <  1:5664711/93‑1 (MQ=255)
                                                            cccATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTAc                             <  1:4562855/93‑1 (MQ=255)
                                                            cccATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTAc                             <  1:2280665/93‑1 (MQ=255)
                                                             ccATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACt                            <  1:1749944/93‑1 (MQ=255)
                                                              cATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTg                           <  1:3749010/93‑1 (MQ=255)
                                                               aTGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGa                          <  1:5696537/93‑1 (MQ=255)
                                                               aTGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGa                          <  2:688603/93‑1 (MQ=255)
                                                               aTGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGa                          >  1:4722634/1‑93 (MQ=255)
                                                                tGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCCCAAATTTATTGaa                         >  1:2087544/1‑93 (MQ=255)
                                                                tGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGaa                         <  2:5526701/93‑1 (MQ=255)
                                                                 gATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGaaa                        <  2:6383537/93‑1 (MQ=255)
                                                                  aTTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAAt                       >  1:5025166/1‑93 (MQ=255)
                                                                    ttAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCt                     <  2:5725228/93‑1 (MQ=255)
                                                                     tAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCtt                    <  1:722376/93‑1 (MQ=255)
                                                                     tAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCtt                    >  1:2189236/1‑93 (MQ=255)
                                                                           aaCCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCagag              <  1:4107667/93‑1 (MQ=255)
                                                                           aaCCATGAGAGGCGCCCCCCCACCCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCatag              <  1:4648171/93‑1 (MQ=255)
                                                                            aCCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGt             <  1:3023315/93‑1 (MQ=255)
                                                                             ccATGAGAGGCGGCCCCCCAACCAGAACGGTAATGGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTc            <  1:224910/93‑1 (MQ=255)
                                                                             ccATGAGAGGCGGCCCCCCAACCAGAACGGCAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTc            >  2:3471340/1‑93 (MQ=255)
                                                                               aTGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCAt          <  2:1540077/93‑1 (MQ=255)
                                                                               aTGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCAt          >  1:5102445/1‑93 (MQ=255)
                                                                               aTGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGCTGTAACTAAAGCCACTTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCAt          >  2:2745982/1‑93 (MQ=255)
                                                                                tGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATg         >  2:742586/1‑93 (MQ=255)
                                                                                tGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATg         <  2:6259860/93‑1 (MQ=255)
                                                                                 gagaGGCGCCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTTATgg        <  2:6160677/93‑1 (MQ=255)
                                                                                  agagGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGga       >  1:5991945/1‑93 (MQ=255)
                                                                                  agagGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGga       >  1:52983/1‑93 (MQ=255)
                                                                                  agagGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGga       <  1:5591571/93‑1 (MQ=255)
                                                                                   gagGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGgaa      <  1:2405240/93‑1 (MQ=255)
                                                                                   gagGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGgaa      <  2:3601477/93‑1 (MQ=255)
                                                                                     ggcggcCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGgaaga    >  2:2222161/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAAT  >  NC_000913/3899350‑3899529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAA  >  NC_000913/3899347‑3899532
                                                                                             |                                                                                            
GTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGC                                                                                              <  SRR4302534.5544966/94‑1 (MQ=60)
 TTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCC                                                                                             >  SRR4302534.1243544/1‑94 (MQ=60)
   TTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCC                                                                                           <  SRR4302534.4214750/94‑1 (MQ=60)
     AACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCC                                                                                         <  SRR4302534.3993438/94‑1 (MQ=60)
     AACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCC                                                                                         <  SRR4302534.875761/94‑1 (MQ=60)
       CTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAA                                                                                       >  SRR4302534.563498/1‑94 (MQ=60)
         GCAACTCAACGCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCGTGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACC                                                                                     <  SRR4302534.5137674/94‑1 (MQ=60)
          CAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCA                                                                                    >  SRR4302534.3044318/1‑94 (MQ=60)
          CAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCA                                                                                    >  SRR4302534.2597375/1‑94 (MQ=60)
           AAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAG                                                                                   <  SRR4302534.3343759/94‑1 (MQ=60)
           AAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAG                                                                                   >  SRR4302534.4284509/1‑94 (MQ=60)
            AATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGA                                                                                  <  SRR4302534.1218098/94‑1 (MQ=60)
            AATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGA                                                                                  <  SRR4302534.57177/94‑1 (MQ=60)
             ATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAA                                                                                 <  SRR4302534.492159/94‑1 (MQ=60)
              CCAAACCCGCCGGAGTATTCGTTACCAAAGCGGCACAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGTCGGCCCCCCAACCAGAAC                                                                                <  SRR4302534.2312481/94‑1 (MQ=60)
              ACAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAAC                                                                                <  SRR4302534.5943565/94‑1 (MQ=60)
                AAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGG                                                                              >  SRR4302534.4444746/1‑94 (MQ=60)
                AAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGGGAACCATGAGAGGCGGCCCCCCAACCAGAACGG                                                                              >  SRR4302534.5763437/1‑94 (MQ=60)
                 AACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGT                                                                             <  SRR4302534.4602017/94‑1 (MQ=60)
                  ACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGCA                                                                            >  SRR4302534.6342308/1‑94 (MQ=60)
                    CAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATTAGAGGCGGCCCCCCAACCAGAACGGTAAT                                                                          >  SRR4302534.224865/1‑94 (MQ=60)
                     AGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATA                                                                         >  SRR4302534.2779774/1‑94 (MQ=60)
                     AGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATA                                                                         >  SRR4302534.6310179/1‑94 (MQ=60)
                       CCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCCGCACGGTAATAGC                                                                       >  SRR4302534.4150099/1‑94 (MQ=60)
                       CCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACAATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGC                                                                       <  SRR4302534.5981861/94‑1 (MQ=60)
                        CGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCC                                                                      <  SRR4302534.6466278/94‑1 (MQ=60)
                        CGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCC                                                                      <  SRR4302534.245982/94‑1 (MQ=60)
                        CGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCC                                                                      <  SRR4302534.6104342/94‑1 (MQ=60)
                        CGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACCGTAATAGCC                                                                      >  SRR4302534.693084/1‑94 (MQ=60)
                         GGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCG                                                                     <  SRR4302534.2446283/94‑1 (MQ=60)
                          GAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGT                                                                    >  SRR4302534.4482299/1‑94 (MQ=60)
                          GAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGT                                                                    <  SRR4302534.2086572/94‑1 (MQ=60)
                          GAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGT                                                                    >  SRR4302534.3072485/1‑94 (MQ=60)
                          GAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTAAGGGGAACAATGGGAGGCGGCCCCCAACCCAGAACGGAAAAAGCCGT                                                                    >  SRR4302534.1824001/1‑94 (MQ=53)
                           AGTGTTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTT                                                                   <  SRR4302534.4058843/94‑1 (MQ=60)
                           AGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGGCCCCCCACCAGAACGGTAATAACCGTT                                                                   >  SRR4302534.2168069/1‑94 (MQ=60)
                           AGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTT                                                                   <  SRR4302534.2978633/94‑1 (MQ=60)
                            GTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTG                                                                  <  SRR4302534.1965642/94‑1 (MQ=60)
                            GTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTG                                                                  <  SRR4302534.2820500/94‑1 (MQ=60)
                             TATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGT                                                                 <  SRR4302534.771755/94‑1 (MQ=60)
                             TATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGT                                                                 >  SRR4302534.6253224/1‑94 (MQ=60)
                             TATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGT                                                                 <  SRR4302534.5934583/94‑1 (MQ=60)
                              ATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTA                                                                <  SRR4302534.379291/94‑1 (MQ=60)
                               TTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAA                                                               >  SRR4302534.2127544/1‑94 (MQ=60)
                                TCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAAC                                                              >  SRR4302534.6146642/1‑94 (MQ=60)
                                 CGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACT                                                             >  SRR4302534.5659950/1‑94 (MQ=60)
                                 CGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACT                                                             >  SRR4302534.2717252/1‑94 (MQ=60)
                                  GTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTA                                                            <  SRR4302534.202409/94‑1 (MQ=60)
                                  GTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTA                                                            >  SRR4302534.1813208/1‑94 (MQ=60)
                                  GTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTA                                                            >  SRR4302534.3061483/1‑94 (MQ=60)
                                    TACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAA                                                          <  SRR4302534.5101923/94‑1 (MQ=60)
                                    TACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAA                                                          >  SRR4302534.3372262/1‑94 (MQ=60)
                                    TACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAA                                                          >  SRR4302534.3188800/1‑94 (MQ=60)
                                    TACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAA                                                          >  SRR4302534.4478314/1‑94 (MQ=60)
                                     ACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAG                                                         >  SRR4302534.3464329/1‑94 (MQ=60)
                                       CAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCC                                                       >  SRR4302534.2572097/1‑94 (MQ=60)
                                        AAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTTAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCA                                                      >  SRR4302534.6018410/1‑94 (MQ=60)
                                        AAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCA                                                      <  SRR4302534.5549199/94‑1 (MQ=60)
                                         AAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAA                                                     >  SRR4302534.376354/1‑94 (MQ=60)
                                          AGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAT                                                    <  SRR4302534.1325095/94‑1 (MQ=60)
                                          AGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAT                                                    <  SRR4302534.143707/94‑1 (MQ=60)
                                           GCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATT                                                   <  SRR4302534.3294944/94‑1 (MQ=60)
                                            CGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTT                                                  <  SRR4302534.2639365/94‑1 (MQ=60)
                                            CGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTT                                                  <  SRR4302534.600311/94‑1 (MQ=60)
                                            CGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTT                                                  <  SRR4302534.650816/94‑1 (MQ=60)
                                             GGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTC                                                 >  SRR4302534.507374/1‑94 (MQ=60)
                                             GGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTC                                                 <  SRR4302534.2550597/94‑1 (MQ=60)
                                             GGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAAATAAAGTCAATTTC                                                 >  SRR4302534.5357174/1‑94 (MQ=60)
                                              GCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCA                                                <  SRR4302534.717640/94‑1 (MQ=60)
                                               CCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCAT                                               >  SRR4302534.2319947/1‑94 (MQ=60)
                                               CCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCAT                                               >  SRR4302534.4135244/1‑94 (MQ=60)
                                                CCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATC                                              >  SRR4302534.4740946/1‑94 (MQ=60)
                                                CCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGCTAATAGCCGTTGTAACTAAATCCAATTTCATC                                              >  SRR4302534.1951013/1‑94 (MQ=60)
                                                 CAGAGAAGCAATCGCCCATGATTTAGGTGAACCATGAGAGGGGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATGT                                             >  SRR4302534.1437056/1‑94 (MQ=60)
                                                 CAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAGCCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCT                                             <  SRR4302534.5393986/94‑1 (MQ=60)
                                                 CAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCT                                             >  SRR4302534.6432378/1‑94 (MQ=60)
                                                 CAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCT                                             <  SRR4302534.1316877/94‑1 (MQ=60)
                                                  AGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTT                                            <  SRR4302534.3919766/94‑1 (MQ=60)
                                                  AAAGAACCAATCTCCCATGAGTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGGAACTAAAGCCAATTTCATCTT                                            <  SRR4302534.1728030/94‑1 (MQ=60)
                                                   GAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTT                                           <  SRR4302534.4109754/94‑1 (MQ=60)
                                                   GAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTT                                           <  SRR4302534.2502732/94‑1 (MQ=60)
                                                    AGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTC                                          >  SRR4302534.279550/1‑94 (MQ=60)
                                                     GAACGAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCGCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCA                                         <  SRR4302534.3240698/94‑1 (MQ=60)
                                                     GAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCA                                         >  SRR4302534.1630653/1‑94 (MQ=60)
                                                     GAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCA                                         <  SRR4302534.123129/94‑1 (MQ=60)
                                                     GAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCA                                         >  SRR4302534.4680471/1‑94 (MQ=60)
                                                     GAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCA                                         <  SRR4302534.3605603/94‑1 (MQ=60)
                                                     GAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCA                                         <  SRR4302534.4800140/94‑1 (MQ=60)
                                                       ACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACA                                       >  SRR4302534.2929813/1‑94 (MQ=60)
                                                       ACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACA                                       >  SRR4302534.2454506/1‑94 (MQ=60)
                                                        CCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAA                                      >  SRR4302534.2693373/1‑94 (MQ=60)
                                                        CCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAA                                      <  SRR4302534.1916443/94‑1 (MQ=60)
                                                         CAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAA                                     >  SRR4302534.5926862/1‑94 (MQ=60)
                                                          AATCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAAT                                    >  SRR4302534.620295/1‑94 (MQ=60)
                                                            TCGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTT                                  >  SRR4302534.2246151/1‑94 (MQ=60)
                                                             CGCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTA                                 <  SRR4302534.5818070/94‑1 (MQ=60)
                                                              GCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTAC                                >  SRR4302534.4850203/1‑94 (MQ=60)
                                                              GCCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTAC                                >  SRR4302534.309271/1‑94 (MQ=60)
                                                               CCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACT                               <  SRR4302534.4686559/94‑1 (MQ=60)
                                                               CCCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACT                               <  SRR4302534.2341180/94‑1 (MQ=60)
                                                                CCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTG                              >  SRR4302534.4889560/1‑94 (MQ=60)
                                                                CCATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTG                              <  SRR4302534.1795369/94‑1 (MQ=60)
                                                                 CATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGA                             >  SRR4302534.4850901/1‑94 (MQ=60)
                                                                 CATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGA                             <  SRR4302534.3849709/94‑1 (MQ=60)
                                                                  ATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCCCAAATTTATTGAA                            >  SRR4302534.2142257/1‑94 (MQ=60)
                                                                  ATGATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAA                            <  SRR4302534.5850855/94‑1 (MQ=60)
                                                                    GATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAAT                          >  SRR4302534.5160899/1‑94 (MQ=60)
                                                                    GATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAAT                          <  SRR4302534.5295085/94‑1 (MQ=60)
                                                                     ATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATC                         <  SRR4302534.429821/94‑1 (MQ=60)
                                                                     ATTTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATC                         <  SRR4302534.2709945/94‑1 (MQ=60)
                                                                     ATTTAGGCGAACCATGAGAGGCGGCCCCCCAACCAGAACGGGAATAGCCGTTGTAACTAAAGCCAATTTCATCCTTCATAAATTTACTTAAAAC                         >  SRR4302534.172027/1‑94 (MQ=60)
                                                                       TTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTT                       <  SRR4302534.5151070/94‑1 (MQ=60)
                                                                       TTAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTT                       >  SRR4302534.2247120/1‑94 (MQ=60)
                                                                        TAGTTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTT                      <  SRR4302534.3370417/94‑1 (MQ=60)
                                                                        TAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTT                      >  SRR4302534.3524598/1‑94 (MQ=60)
                                                                        TAGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTT                      <  SRR4302534.740202/94‑1 (MQ=60)
                                                                         AGGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTC                     <  SRR4302534.2378763/94‑1 (MQ=60)
                                                                          GGTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCA                    <  SRR4302534.5845082/94‑1 (MQ=60)
                                                                           GTGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAG                   <  SRR4302534.4221803/94‑1 (MQ=60)
                                                                            TGAACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGA                  >  SRR4302534.5944667/1‑94 (MQ=60)
                                                                              AACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGT                >  SRR4302534.1023428/1‑94 (MQ=60)
                                                                              AACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGT                <  SRR4302534.4218150/94‑1 (MQ=60)
                                                                              AACCATGAGAGGCGCCCCCCCACCCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCATAGT                <  SRR4302534.4774353/94‑1 (MQ=60)
                                                                               ACCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTC               <  SRR4302534.3102531/94‑1 (MQ=60)
                                                                                CCATGAGAGGCGGCCCCCCAACCAGAACGGTAATGGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCA              <  SRR4302534.230781/94‑1 (MQ=60)
                                                                                CCATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCA              <  SRR4302534.1219647/94‑1 (MQ=60)
                                                                                 CATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCAT             >  SRR4302534.5240168/1‑94 (MQ=60)
                                                                                  ATGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATG            <  SRR4302534.2012457/94‑1 (MQ=60)
                                                                                   TGAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGG           >  SRR4302534.4008652/1‑94 (MQ=60)
                                                                                    GAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGA          >  SRR4302534.6154722/1‑94 (MQ=60)
                                                                                    GAGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGA          >  SRR4302534.54403/1‑94 (MQ=60)
                                                                                     AGAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAA         <  SRR4302534.5742739/94‑1 (MQ=60)
                                                                                      GAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAG        <  SRR4302534.2469166/94‑1 (MQ=60)
                                                                                      GAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAG        >  SRR4302534.5163087/1‑94 (MQ=60)
                                                                                      GAGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAG        <  SRR4302534.5420495/94‑1 (MQ=60)
                                                                                       AGGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGA       >  SRR4302534.261290/1‑94 (MQ=60)
                                                                                        GGCGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAA      >  SRR4302534.4259819/1‑94 (MQ=60)
                                                                                          CGGCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATA    >  SRR4302534.6153350/1‑94 (MQ=60)
                                                                                            GCCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAA  <  SRR4302534.1910300/94‑1 (MQ=60)
                                                                                             |                                                                                            
GTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGAATAAA  >  NC_000913/3899347‑3899532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑