Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,066,222	T→C	Y212C (TAC→TGC)	yihQ ←	putative glycosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,066,222	0	T	C	100.0%	954.1 / NA	269	Y212C (TAC→TGC)	yihQ	putative glycosidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (131/138); total (131/138)

Only 100 of 272 total aligned reads displayed.

AGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGTATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTGGG  >  NC_000913/4066132‑4066311

agGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCAt                                                                                         <  2:781503/93‑1 (MQ=255)

 gggCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCAtt                                                                                        >  1:3093915/1‑93 (MQ=255)

 gggCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCAtt                                                                                        <  1:5296904/93‑1 (MQ=255)

 gggCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCAtt                                                                                        <  1:3684929/93‑1 (MQ=255)

   gCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTcc                                                                                      <  2:3612600/93‑1 (MQ=255)

   gCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTcc                                                                                      <  1:4600665/93‑1 (MQ=255)

   gCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTcc                                                                                      >  2:5927580/1‑93 (MQ=255)

     ggTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCgg                                                                                    >  2:2389549/1‑93 (MQ=255)

     ggTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCgg                                                                                    <  2:6314196/93‑1 (MQ=255)

      gTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCggg                                                                                   >  1:320984/1‑93 (MQ=255)

         aaTTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCa                                                                                >  1:6103488/1‑93 (MQ=255)

          aTTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCAc                                                                               <  1:4593145/93‑1 (MQ=255)

          aTTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCAc                                                                               >  2:4466137/1‑93 (MQ=255)

          aTTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCAc                                                                               <  2:6096490/93‑1 (MQ=255)

           tttttCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACt                                                                              >  2:471044/1‑93 (MQ=255)

            ttttCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCGCAGCGCCAGTTCATGGCATTCCGGGGCACTa                                                                             <  1:5499640/93‑1 (MQ=255)

             tttCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTaa                                                                            >  1:1902819/1‑93 (MQ=255)

               tCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAg                                                                          <  2:890235/93‑1 (MQ=255)

                ccAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGt                                                                         <  2:5709159/93‑1 (MQ=255)

                ccAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGt                                                                         <  1:5068510/93‑1 (MQ=255)

                 cagcagGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTc                                                                        <  1:421802/93‑1 (MQ=255)

                   gcagGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGa                                                                      >  1:5995070/1‑93 (MQ=255)

                   gcagGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGa                                                                      >  2:5082003/1‑93 (MQ=255)

                    cagGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGaa                                                                     <  2:144512/93‑1 (MQ=255)

                    cagGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGaa                                                                     <  2:3057063/93‑1 (MQ=255)

                      gggAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCTAAGt                                                                   >  1:2565752/1‑93 (MQ=255)

                      gggAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGt                                                                   <  1:4079480/93‑1 (MQ=255)

                       ggAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGtt                                                                  <  1:4870349/93‑1 (MQ=255)

                        gAAATGTATGTGTCGGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTc                                                                 <  1:1250682/93‑1 (MQ=255)

                        gAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTc                                                                 <  2:5323809/93‑1 (MQ=255)

                          aaTGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCat                                                               >  2:298824/1‑93 (MQ=255)

                           atgtatgtGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCata                                                              >  2:5461127/1‑93 (MQ=255)

                            tgtatgtGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCatat                                                             >  2:2546133/1‑93 (MQ=255)

                            tgtatgtGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCatat                                                             <  1:5861991/93‑1 (MQ=255)

                              tatgtGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAg                                                           >  1:3330645/1‑93 (MQ=255)

                                   gtCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACt                                                      <  2:5618648/93‑1 (MQ=255)

                                    tCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTg                                                     <  1:743141/93‑1 (MQ=255)

                                    tCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTg                                                     >  2:5445117/1‑93 (MQ=255)

                                      aGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGtt                                                   <  1:5389272/93‑1 (MQ=255)

                                       gCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGttt                                                  >  1:2302897/1‑92 (MQ=255)

                                       gCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTa                                                  >  1:5194782/1‑93 (MQ=255)

                                       gCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTa                                                  >  2:3664457/1‑93 (MQ=255)

                                       gCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTa                                                  >  1:3948479/1‑93 (MQ=255)

                                         acaTTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTAta                                                >  2:2754964/1‑92 (MQ=255)

                                         acaTTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATc                                                >  2:3375160/1‑93 (MQ=255)

                                         acaTTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATc                                                >  2:178415/1‑93 (MQ=255)

                                          caTTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCa                                               >  2:4987685/1‑93 (MQ=255)

                                            ttCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAAc                                             >  2:4481973/1‑93 (MQ=255)

                                            ttCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAAc                                             <  2:4657829/93‑1 (MQ=255)

                                            ttCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAAc                                             >  2:4977519/1‑93 (MQ=255)

                                            ttCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAAc                                             >  1:1288266/1‑93 (MQ=255)

                                             tCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACa                                            >  2:4419196/1‑93 (MQ=255)

                                              cAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACAt                                           <  1:5826168/93‑1 (MQ=255)

                                              cAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACAt                                           <  2:3073514/93‑1 (MQ=255)

                                              cAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACAt                                           <  1:651957/93‑1 (MQ=255)

                                              cAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACAt                                           >  2:4878452/1‑93 (MQ=255)

                                              cAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACAt                                           <  2:28390/93‑1 (MQ=255)

                                               aaaaCGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATg                                          <  2:2642361/93‑1 (MQ=255)

                                                 aaCGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGc                                        <  2:1167762/93‑1 (MQ=255)

                                                  aCGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCa                                       >  1:2701804/1‑93 (MQ=255)

                                                  aCGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCa                                       <  2:3171407/93‑1 (MQ=255)

                                                  aCGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCa                                       <  1:2879560/93‑1 (MQ=255)

                                                      aGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTaa                                   >  1:3836880/1‑93 (MQ=255)

                                                       gCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAAt                                  >  1:1988752/1‑93 (MQ=255)

                                                       gCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAAt                                  >  2:6026987/1‑93 (MQ=255)

                                                         gTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATAc                                >  1:426903/1‑93 (MQ=255)

                                                          ttGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACt                               <  1:2157928/93‑1 (MQ=255)

                                                          ttGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACt                               >  2:3584112/1‑93 (MQ=255)

                                                          ttGCTTTGTCTTCCCACAGCGCCAGTGCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACt                               <  2:2235019/93‑1 (MQ=255)

                                                           tGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACtt                              <  2:1680194/93‑1 (MQ=255)

                                                           tGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACtt                              <  2:1354249/93‑1 (MQ=255)

                                                           tGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACtt                              <  2:647441/93‑1 (MQ=255)

                                                            gCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTc                             <  1:4312744/93‑1 (MQ=255)

                                                            gCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTc                             >  1:4449878/1‑93 (MQ=255)

                                                               ttGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGc                          <  2:5544913/93‑1 (MQ=255)

                                                               ttGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGc                          >  1:6328862/1‑93 (MQ=255)

                                                                tGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCg                         >  2:5270607/1‑93 (MQ=255)

                                                                 gTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGt                        <  2:5788877/93‑1 (MQ=255)

                                                                 gTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGt                        <  1:1501299/93‑1 (MQ=255)

                                                                 gTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGt                        >  1:581534/1‑93 (MQ=255)

                                                                 gTCTTCCCACAGCGCCAGTTCAT‑GCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTg                       >  1:6153120/1‑93 (MQ=255)

                                                                  gcTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTg                       <  2:187013/92‑1 (MQ=255)

                                                                    ttCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCt                     >  1:6444924/1‑93 (MQ=255)

                                                                    ttCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCt                     >  1:362475/1‑93 (MQ=255)

                                                                       ccACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGAc                  <  2:4751422/93‑1 (MQ=255)

                                                                       ccACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGAc                  >  1:5605016/1‑93 (MQ=255)

                                                                            gcgcCAGTTCATGGCATTCCGGGTCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACg             <  1:3777977/93‑1 (MQ=255)

                                                                            gcgcCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACg             <  2:4027041/93‑1 (MQ=255)

                                                                             cgcCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGt            <  2:5308524/93‑1 (MQ=255)

                                                                              gcCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTa           <  2:1587077/93‑1 (MQ=255)

                                                                              gcCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTa           >  1:941769/1‑93 (MQ=255)

                                                                              gcCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTa           >  2:2013532/1‑93 (MQ=255)

                                                                               ccAGTTCATGGTATTCCGGTGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAg          <  1:4525547/93‑1 (MQ=255)

                                                                                  gTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCt       >  2:5743201/1‑93 (MQ=255)

                                                                                  gTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCt       >  1:2064217/1‑93 (MQ=255)

                                                                                    tCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCtgt     >  1:1751473/1‑93 (MQ=255)

                                                                                     cATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCtgtg    >  2:4340578/1‑93 (MQ=255)

                                                                                     cATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCtgtg    >  2:677197/1‑93 (MQ=255)

                                                                                     cATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCtgtg    >  2:701977/1‑93 (MQ=255)

                                                                                      aTGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTgg   >  2:11892/1‑93 (MQ=255)

AGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGTATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTGGG  >  NC_000913/4066132‑4066311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCAGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGTATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTGGGAAGA  >  NC_000913/4066130‑4066315

GCAGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCA                                                                                              <  SRR4302534.4705514/94‑1 (MQ=60)

 CAGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCAT                                                                                             >  SRR4302534.4720678/1‑94 (MQ=60)

  AGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATT                                                                                            >  SRR4302534.3175302/1‑94 (MQ=60)

  AGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATT                                                                                            >  SRR4302534.6232736/1‑94 (MQ=60)

   GGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTC                                                                                           >  SRR4302534.3896848/1‑94 (MQ=60)

   GGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTC                                                                                           <  SRR4302534.5439860/94‑1 (MQ=60)

   GGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTC                                                                                           <  SRR4302534.3784002/94‑1 (MQ=60)

     GCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCG                                                                                         <  SRR4302534.4725528/94‑1 (MQ=60)

     GCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCG                                                                                         <  SRR4302534.5846843/94‑1 (MQ=60)

       GGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGG                                                                                       >  SRR4302534.329246/1‑94 (MQ=60)

          TAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCA                                                                                    >  SRR4302534.6269456/1‑94 (MQ=60)

          TAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCA                                                                                    <  SRR4302534.2108665/94‑1 (MQ=60)

          TAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCA                                                                                    <  SRR4302534.4769554/94‑1 (MQ=60)

           AATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCAC                                                                                   <  SRR4302534.5498390/94‑1 (MQ=60)

            ATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACT                                                                                  <  SRR4302534.4717803/94‑1 (MQ=60)

              TTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCGCAGCGCCAGTTCATGGCATTCCGGGGCACTAA                                                                                <  SRR4302534.5647955/94‑1 (MQ=60)

              TTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAA                                                                                >  SRR4302534.1952450/1‑94 (MQ=60)

                TTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAG                                                                              <  SRR4302534.3333777/94‑1 (MQ=60)

                TTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGATTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAG                                                                              <  SRR4302534.2953365/94‑1 (MQ=60)

                  CCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTC                                                                            <  SRR4302534.5205348/94‑1 (MQ=60)

                  CCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTC                                                                            <  SRR4302534.1701336/94‑1 (MQ=60)

                   CAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCG                                                                           <  SRR4302534.432586/94‑1 (MQ=60)

                    AGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGA                                                                          >  SRR4302534.6157933/1‑94 (MQ=60)

                     GCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAA                                                                         <  SRR4302534.2994266/94‑1 (MQ=60)

                      CAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAG                                                                        <  SRR4302534.2197027/94‑1 (MQ=60)

                      CAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAG                                                                        >  SRR4302534.5372995/1‑94 (MQ=60)

                      CAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAG                                                                        <  SRR4302534.4270631/94‑1 (MQ=60)

                      CAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAG                                                                        <  SRR4302534.6356594/94‑1 (MQ=60)

                       AGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCTAAGT                                                                       >  SRR4302534.2633745/1‑94 (MQ=60)

                       AGGGAAATGTATGTGTCAGCAAATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGT                                                                       >  SRR4302534.4403883/1‑94 (MQ=60)

                        GGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTT                                                                      <  SRR4302534.4188979/94‑1 (MQ=60)

                         GGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTC                                                                     <  SRR4302534.5002445/94‑1 (MQ=60)

                          GAAATGTATGTGTCGGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCA                                                                    <  SRR4302534.1283648/94‑1 (MQ=60)

                          GAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCA                                                                    <  SRR4302534.5500168/94‑1 (MQ=60)

                           AAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCAT                                                                   <  SRR4302534.1638868/94‑1 (MQ=60)

                           AAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCAT                                                                   >  SRR4302534.5973812/1‑94 (MQ=60)

                              TGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATA                                                                <  SRR4302534.3845199/94‑1 (MQ=60)

                              TGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATA                                                                <  SRR4302534.6021151/94‑1 (MQ=60)

                               GTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAG                                                               >  SRR4302534.4418602/1‑94 (MQ=60)

                               GTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAG                                                               <  SRR4302534.4575209/94‑1 (MQ=60)

                               GTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAG                                                               >  SRR4302534.5578223/1‑94 (MQ=60)

                               GTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAG                                                               >  SRR4302534.3419726/1‑94 (MQ=60)

                                TATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGC                                                              >  SRR4302534.1527868/1‑94 (MQ=60)

                                 ATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCA                                                             <  SRR4302534.4989897/94‑1 (MQ=60)

                                  TGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAA                                                            <  SRR4302534.6009556/94‑1 (MQ=60)

                                      TCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGT                                                        <  SRR4302534.471614/94‑1 (MQ=60)

                                      TCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGT                                                        <  SRR4302534.761552/94‑1 (MQ=60)

                                        AGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTT                                                      >  SRR4302534.2364025/1‑94 (MQ=60)

                                        AGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTA                                                      >  SRR4302534.4054189/1‑94 (MQ=60)

                                        AGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTA                                                      <  SRR4302534.5534417/94‑1 (MQ=60)

                                        AGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTA                                                      >  SRR4302534.4332508/1‑94 (MQ=60)

                                        AGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTA                                                      <  SRR4302534.845146/94‑1 (MQ=60)

                                        AGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTA                                                      >  SRR4302534.5334867/1‑94 (MQ=60)

                                        AGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTA                                                      <  SRR4302534.3731002/94‑1 (MQ=60)

                                         GCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTAT                                                     <  SRR4302534.6097695/94‑1 (MQ=60)

                                         GCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTAT                                                     >  SRR4302534.879326/1‑94 (MQ=60)

                                          CACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATC                                                    >  SRR4302534.2419010/1‑94 (MQ=60)

                                           ACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCA                                                   <  SRR4302534.4150412/94‑1 (MQ=60)

                                            CATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAA                                                  >  SRR4302534.4649478/1‑94 (MQ=60)

                                            CATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAA                                                  >  SRR4302534.5223618/1‑94 (MQ=60)

                                             ATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAAC                                                 >  SRR4302534.6279465/1‑94 (MQ=60)

                                             ATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAAC                                                 >  SRR4302534.1322192/1‑94 (MQ=60)

                                              TTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAAGA                                                <  SRR4302534.6370566/94‑1 (MQ=60)

                                               TCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACAT                                               <  SRR4302534.5281766/94‑1 (MQ=60)

                                               TCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACAT                                               <  SRR4302534.1286684/94‑1 (MQ=60)

                                                CAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATG                                              <  SRR4302534.5984314/94‑1 (MQ=60)

                                                CAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATG                                              <  SRR4302534.667794/94‑1 (MQ=60)

                                                 AAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGG                                             >  SRR4302534.62017/1‑94 (MQ=60)

                                                   AACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCA                                           <  SRR4302534.2142418/94‑1 (MQ=60)

                                                   AACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCA                                           >  SRR4302534.2773169/1‑94 (MQ=60)

                                                    ACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAG                                          <  SRR4302534.2955131/94‑1 (MQ=60)

                                                     CGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGT                                         >  SRR4302534.4280485/1‑94 (MQ=60)

                                                      GCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTA                                        <  SRR4302534.204800/94‑1 (MQ=60)

                                                       CAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAA                                       >  SRR4302534.3939938/1‑94 (MQ=60)

                                                       CAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAA                                       >  SRR4302534.2024830/1‑94 (MQ=60)

                                                       CAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAA                                       <  SRR4302534.2260821/94‑1 (MQ=60)

                                                        AGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAAT                                      >  SRR4302534.599337/1‑94 (MQ=60)

                                                        AGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAAT                                      >  SRR4302534.2040830/1‑94 (MQ=60)

                                                         GCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATA                                     <  SRR4302534.3738027/94‑1 (MQ=60)

                                                          CGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATAC                                    >  SRR4302534.3413123/1‑94 (MQ=60)

                                                          CGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATAC                                    <  SRR4302534.6608945/94‑1 (MQ=60)

                                                          CGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATAC                                    >  SRR4302534.437802/1‑94 (MQ=60)

                                                           GTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACT                                   >  SRR4302534.1897506/1‑94 (MQ=60)

                                                           GTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACT                                   <  SRR4302534.4489690/94‑1 (MQ=60)

                                                           GTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACT                                   <  SRR4302534.5728492/94‑1 (MQ=60)

                                                            TTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTT                                  <  SRR4302534.2214900/94‑1 (MQ=60)

                                                            TTGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTT                                  >  SRR4302534.3281579/1‑94 (MQ=60)

                                                             TGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTC                                 >  SRR4302534.4570143/1‑94 (MQ=60)

                                                             TGCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTC                                 >  SRR4302534.1514002/1‑94 (MQ=60)

                                                              GCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCT                                <  SRR4302534.6155095/94‑1 (MQ=60)

                                                              GCTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCT                                <  SRR4302534.4429570/94‑1 (MQ=60)

                                                               CTTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTG                               <  SRR4302534.2486735/94‑1 (MQ=60)

                                                                TTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGC                              <  SRR4302534.267316/94‑1 (MQ=60)

                                                                TTTGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGC                              >  SRR4302534.6499849/1‑94 (MQ=60)

                                                                  TGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGT                            >  SRR4302534.5867453/1‑94 (MQ=60)

                                                                  TGTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGT                            >  SRR4302534.595771/1‑94 (MQ=60)

                                                                  TGTCTTCCCACAGCGCCAGTTCAT‑GCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTG                           >  SRR4302534.6320509/1‑94 (MQ=60)

                                                                   GTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTG                           <  SRR4302534.1540813/94‑1 (MQ=60)

                                                                   GTCTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTG                           <  SRR4302534.4644916/94‑1 (MQ=60)

                                                                     CTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCT                         >  SRR4302534.5375410/1‑94 (MQ=60)

                                                                     CTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCT                         >  SRR4302534.3284155/1‑94 (MQ=60)

                                                                     CTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCT                         >  SRR4302534.371716/1‑94 (MQ=60)

                                                                     CTTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCT                         >  SRR4302534.6618602/1‑94 (MQ=60)

                                                                      TTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTG                        <  SRR4302534.742642/94‑1 (MQ=60)

                                                                      TTCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTG                        <  SRR4302534.5194470/94‑1 (MQ=60)

                                                                       TCCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGA                       >  SRR4302534.75497/1‑94 (MQ=60)

                                                                        CCCACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGAC                      >  SRR4302534.5756615/1‑94 (MQ=60)

                                                                           ACAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAA                   >  SRR4302534.2009522/1‑94 (MQ=60)

                                                                            CAGCGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAAC                  <  SRR4302534.6548010/94‑1 (MQ=60)

                                                                              GCGCCAGTTCATGGCATTCCGGGTCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGT                <  SRR4302534.3879400/94‑1 (MQ=60)

                                                                               CGCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTA               >  SRR4302534.965723/1‑94 (MQ=60)

                                                                                GCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAG              >  SRR4302534.2757173/1‑94 (MQ=60)

                                                                                GCCAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAG              >  SRR4302534.401688/1‑94 (MQ=60)

                                                                                 CCAGTTCATGGTATTCCGGTGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGG             <  SRR4302534.4648145/94‑1 (MQ=60)

                                                                                  CAGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGC            <  SRR4302534.3840379/94‑1 (MQ=60)

                                                                                   AGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCT           >  SRR4302534.2118192/1‑94 (MQ=60)

                                                                                   AGTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCT           <  SRR4302534.4530565/94‑1 (MQ=60)

                                                                                    GTTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTG          >  SRR4302534.3973115/1‑94 (MQ=60)

                                                                                     TTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGT         >  SRR4302534.1796946/1‑94 (MQ=60)

                                                                                     TTCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGT         >  SRR4302534.5701840/1‑94 (MQ=60)

                                                                                      TCATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTG        >  SRR4302534.2975117/1‑94 (MQ=60)

                                                                                       CATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTGG       >  SRR4302534.406784/1‑94 (MQ=60)

                                                                                        ATGGCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTGGG      >  SRR4302534.3686532/1‑94 (MQ=60)

                                                                                        ATGGCATTCCGAGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTGGG      <  SRR4302534.6650338/94‑1 (MQ=60)

                                                                                           GCATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTGGGAAG   <  SRR4302534.5849648/94‑1 (MQ=60)

                                                                                            CATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTGGGAAGA  >  SRR4302534.2857020/1‑94 (MQ=60)

GCAGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGTATTCCGGGGCACTAAAGTCGAAGTTCATATAGCAACTGTTATCAACATGGCAGTAATACTTCTGCGTGCTGACAAACGTAGGCTGTGGGAAGA  >  NC_000913/4066130‑4066315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`