Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,132,580	T→A	I389N (ATC→AAC)	katG →	catalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,132,580	0	T	A	98.8%	927.7 / ‑6.2	262	I389N (ATC→AAC)	katG	catalase
Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (156/100); minor base G (0/3); total (156/106)
Fisher's exact test for biased strand distribution p-value = 6.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

TCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGA  >  NC_000913/4132490‑4132669

tCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAact                                                                                         >  1:4154136/1‑93 (MQ=255)

 cccGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAactc                                                                                        >  1:5480024/1‑93 (MQ=255)

  ccGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGTAGAactct                                                                                       <  1:6363256/93‑1 (MQ=255)

  ccGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAactct                                                                                       >  2:319818/1‑93 (MQ=255)

   cGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAactctc                                                                                      >  2:1263639/1‑93 (MQ=255)

    ggATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCtcg                                                                                     >  2:1866420/1‑93 (MQ=255)

     gATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCtcgt                                                                                    <  1:2924801/93‑1 (MQ=255)

      aTCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGCCCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCtcgtc                                                                                   <  1:4623405/93‑1 (MQ=255)

      aTCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCtcgtc                                                                                   >  1:1595209/1‑93 (MQ=255)

      aTCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCtcgtc                                                                                   <  2:4954072/93‑1 (MQ=255)

      aTCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCtcgtc                                                                                   >  2:22095/1‑93 (MQ=255)

        ccGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTcgtcgt                                                                                 >  2:324093/1‑93 (MQ=255)

           tttGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTc                                                                              >  1:3820542/1‑93 (MQ=255)

            ttGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTcc                                                                             <  1:4440315/93‑1 (MQ=255)

             tGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCt                                                                            <  2:62811/93‑1 (MQ=255)

                tCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCaa                                                                         >  1:6288874/1‑93 (MQ=255)

                 ccGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAAc                                                                        <  2:3415448/93‑1 (MQ=255)

                  cGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACg                                                                       >  2:5436025/1‑93 (MQ=255)

                  cGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACg                                                                       <  1:5212479/93‑1 (MQ=255)

                   gTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGa                                                                      <  2:3512254/93‑1 (MQ=255)

                    tCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCCACGAt                                                                     >  1:4586175/1‑93 (MQ=255)

                    tCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGAt                                                                     <  2:4824297/93‑1 (MQ=255)

                      gaagaaACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATcc                                                                   >  1:6158748/1‑93 (MQ=255)

                      gaagaaACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATcc                                                                   <  1:6014378/93‑1 (MQ=255)

                       aagaaACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCg                                                                  <  1:2939326/93‑1 (MQ=255)

                       aagaaACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCg                                                                  >  2:5770925/1‑93 (MQ=255)

                        agaaACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGc                                                                 <  1:5094891/93‑1 (MQ=255)

                        agaaACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGc                                                                 <  2:5521657/93‑1 (MQ=255)

                         gaaACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCa                                                                >  2:1411919/1‑93 (MQ=255)

                         gaaACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCa                                                                <  1:2066135/93‑1 (MQ=255)

                          aaaCGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAg                                                               >  2:6383759/1‑93 (MQ=255)

                          aaaCGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAg                                                               <  1:1172345/93‑1 (MQ=255)

                           aaCGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAgg                                                              >  1:3575103/1‑93 (MQ=255)

                            aCGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGc                                                             <  2:2752064/93‑1 (MQ=255)

                              gTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGt                                                           <  1:1120674/93‑1 (MQ=255)

                              gTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGt                                                           <  1:1372079/93‑1 (MQ=255)

                              gTAAACCGACAATGCTGGTGACCAACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGt                                                           >  2:1051439/1‑93 (MQ=255)

                                aaaCCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTc                                                         >  1:2236666/1‑93 (MQ=255)

                                aaaCCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTc                                                         >  1:273067/1‑93 (MQ=255)

                                aaaCCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTc                                                         >  2:4186729/1‑93 (MQ=255)

                                 aaCCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCa                                                        >  1:299165/1‑93 (MQ=255)

                                 aaCCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCa                                                        <  2:5619565/93‑1 (MQ=255)

                                  aCCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCaa                                                       >  2:2135667/1‑93 (MQ=255)

                                    cGACAATGCTGGTGACCGACCTGACGCTGCTTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACg                                                     <  2:5087433/93‑1 (MQ=255)

                                    cGACAATGCTGGTGACCGACCTGACGCTGCG‑TTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGa                                                    <  1:1537529/93‑1 (MQ=255)

                                     gACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGa                                                    >  2:386142/1‑93 (MQ=255)

                                     gACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGa                                                    >  2:1810977/1‑93 (MQ=255)

                                      aCAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGaa                                                   <  1:3452559/93‑1 (MQ=255)

                                      aCAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGaa                                                   >  2:824029/1‑93 (MQ=255)

                                      aCAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGaa                                                   >  1:4909112/1‑93 (MQ=255)

                                       cAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAg                                                  <  2:1162263/93‑1 (MQ=255)

                                         aTGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCCACGATCCGCAGGCGTTCAACGaacct                                                >  2:2343268/1‑90 (MQ=255)

                                         aTGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGcc                                                >  1:4739854/1‑93 (MQ=255)

                                          tGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCt                                               >  2:4897154/1‑93 (MQ=255)

                                            cTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCttt                                             >  2:13384/1‑93 (MQ=255)

                                            cTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCttt                                             <  1:3394929/93‑1 (MQ=255)

                                            cTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCttt                                             <  1:3999034/93‑1 (MQ=255)

                                               gTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGcc                                          <  1:3871043/93‑1 (MQ=255)

                                                tcaccgaccTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGccc                                         <  2:1556807/91‑1 (MQ=255)

                                                tGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGccc                                         <  2:685134/93‑1 (MQ=255)

                                                  accgaccTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGt                                       >  2:5732164/1‑93 (MQ=255)

                                                  accgaccTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGt                                       >  1:2696040/1‑93 (MQ=255)

                                                   ccgaccTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTg                                      >  1:1798274/1‑93 (MQ=255)

                                                     gaccTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGcc                                    >  1:2549963/1‑93 (MQ=255)

                                                     gaccTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGcc                                    <  1:4987641/93‑1 (MQ=255)

                                                      accTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCt                                   <  2:3069180/93‑1 (MQ=255)

                                                      accTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCt                                   >  2:2401731/1‑93 (MQ=255)

                                                      accTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCt                                   >  1:720245/1‑93 (MQ=255)

                                                      accTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCt                                   >  1:5317851/1‑93 (MQ=255)

                                                       ccTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTg                                  <  1:5045962/93‑1 (MQ=255)

                                                         tGACGCTGCGTTTTGAGCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGt                                <  1:1700321/93‑1 (MQ=255)

                                                          gACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGtt                               >  1:2406412/1‑93 (MQ=255)

                                                          gACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGtt                               <  2:3459128/93‑1 (MQ=255)

                                                          gACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGtt                               <  2:2786995/93‑1 (MQ=255)

                                                          gACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGtt                               >  2:4731106/1‑93 (MQ=255)

                                                            cGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCa                             >  2:5942376/1‑93 (MQ=255)

                                                             gCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCaa                            >  2:6440055/1‑93 (MQ=255)

                                                                  gTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGa                       >  1:377370/1‑93 (MQ=255)

                                                                  gTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGa                       >  1:1688272/1‑93 (MQ=255)

                                                                   ttttGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGAc                      >  1:5634055/1‑93 (MQ=255)

                                                                       gATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGcac                  <  2:920936/93‑1 (MQ=255)

                                                                       gATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGcac                  >  2:2592218/1‑93 (MQ=255)

                                                                        aTCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGcaca                 >  2:4566911/1‑93 (MQ=255)

                                                                        aTCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGcaca                 >  2:1932216/1‑93 (MQ=255)

                                                                        aTCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGcaca                 >  2:6109382/1‑93 (MQ=255)

                                                                          ccTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAgg               >  2:3943051/1‑93 (MQ=255)

                                                                          ccTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAgg               >  2:3627395/1‑93 (MQ=255)

                                                                           cTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAggg              <  1:1964033/93‑1 (MQ=255)

                                                                            tGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGa             >  2:5276010/1‑93 (MQ=255)

                                                                            tGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGa             <  2:3359143/93‑1 (MQ=255)

                                                                              aGTTCGAGAAGAACGCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGata           <  2:5218229/93‑1 (MQ=255)

                                                                               gTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGatat          >  1:972600/1‑93 (MQ=255)

                                                                               gTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGatat          >  2:2616396/1‑93 (MQ=255)

                                                                                ttCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATg         <  2:4348340/93‑1 (MQ=255)

                                                                                   gagaAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATgggg      <  2:1766316/93‑1 (MQ=255)

                                                                                      aagaACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCg   >  2:4062587/1‑93 (MQ=255)

                                                                                      aagaACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCg   >  1:4338402/1‑93 (MQ=255)

                                                                                      aagaACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCg   <  1:2473212/93‑1 (MQ=255)

                                                                                       agaACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGa  <  1:5878934/93‑1 (MQ=255)

                                                                                       agaACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGCATATGGGGCCGa  <  1:6184644/93‑1 (MQ=255)

TCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGA  >  NC_000913/4132490‑4132669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATC  >  NC_000913/4132488‑4132673

TATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAAC                                                                                              >  SRR4302534.6230751/1‑94 (MQ=60)

TATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAAC                                                                                              >  SRR4302534.4976074/1‑94 (MQ=60)

 ATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACT                                                                                             <  SRR4302534.4968025/94‑1 (MQ=60)

 ATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACT                                                                                             >  SRR4302534.5130079/1‑94 (MQ=60)

 ATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACT                                                                                             >  SRR4302534.4266286/1‑94 (MQ=60)

  TCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTC                                                                                            >  SRR4302534.5627806/1‑94 (MQ=60)

   CCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCT                                                                                           <  SRR4302534.4820066/94‑1 (MQ=60)

    CCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGTAGAACTCTC                                                                                          <  SRR4302534.6535075/94‑1 (MQ=60)

    CCGGATCCGTTTGATCATTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTC                                                                                          <  SRR4302534.3940178/94‑1 (MQ=60)

     CGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCA                                                                                         >  SRR4302534.6123516/1‑94 (MQ=60)

      GGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACATGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGT                                                                                        >  SRR4302534.6063188/1‑94 (MQ=60)

       GATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTC                                                                                       <  SRR4302534.3001569/94‑1 (MQ=60)

       GATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTC                                                                                       >  SRR4302534.1636899/1‑94 (MQ=60)

       GATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTC                                                                                       >  SRR4302534.3014316/1‑94 (MQ=60)

       GATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTC                                                                                       >  SRR4302534.400896/1‑94 (MQ=60)

       GATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTC                                                                                       >  SRR4302534.4898886/1‑94 (MQ=60)

        ATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGCCCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCG                                                                                      <  SRR4302534.4748853/94‑1 (MQ=60)

        ATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCG                                                                                      >  SRR4302534.4477679/1‑94 (MQ=60)

            GTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTC                                                                                  >  SRR4302534.3923183/1‑94 (MQ=60)

             TTCGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGTCCGACCTGACGCTGCGTTTTTATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCC                                                                                 <  SRR4302534.2808951/94‑1 (MQ=60)

              TTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCT                                                                                <  SRR4302534.4560265/94‑1 (MQ=60)

               TGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTC                                                                               <  SRR4302534.1902831/94‑1 (MQ=60)

               TGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTC                                                                               >  SRR4302534.4465745/1‑94 (MQ=60)

                GATCCGTCGAAGAAACGTACACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCA                                                                              <  SRR4302534.5493733/94‑1 (MQ=60)

                GATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCA                                                                              >  SRR4302534.5658603/1‑94 (MQ=60)

                 ATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAA                                                                             >  SRR4302534.4561727/1‑94 (MQ=60)

                 ATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAA                                                                             >  SRR4302534.6459021/1‑94 (MQ=60)

                  TCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAAC                                                                            <  SRR4302534.539971/94‑1 (MQ=60)

                  TCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAAC                                                                            <  SRR4302534.1660207/94‑1 (MQ=60)

                  TCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAAC                                                                            >  SRR4302534.2439746/1‑94 (MQ=60)

                   CCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACG                                                                           >  SRR4302534.4740783/1‑94 (MQ=60)

                   CCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACG                                                                           <  SRR4302534.4673752/94‑1 (MQ=60)

                    CGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGA                                                                          <  SRR4302534.5353153/94‑1 (MQ=60)

                    CGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGA                                                                          >  SRR4302534.1640796/1‑94 (MQ=60)

                     GTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCCACGAT                                                                         >  SRR4302534.4710625/1‑94 (MQ=60)

                     GTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGAT                                                                         <  SRR4302534.3705337/94‑1 (MQ=60)

                     CTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGAT                                                                         <  SRR4302534.1733285/94‑1 (MQ=60)

                       CGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCC                                                                       >  SRR4302534.2822668/1‑94 (MQ=60)

                       CGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCC                                                                       >  SRR4302534.665858/1‑94 (MQ=60)

                       CGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCC                                                                       >  SRR4302534.6326255/1‑94 (MQ=60)

                        GAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCG                                                                      >  SRR4302534.3877198/1‑94 (MQ=60)

                        GAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCG                                                                      <  SRR4302534.1802821/94‑1 (MQ=60)

                        GAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCG                                                                      <  SRR4302534.6177798/94‑1 (MQ=60)

                        GAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCC                                                                      >  SRR4302534.6249099/1‑94 (MQ=60)

                         AAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGC                                                                     >  SRR4302534.4434699/1‑94 (MQ=60)

                         AAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGC                                                                     <  SRR4302534.3016467/94‑1 (MQ=60)

                          AGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCA                                                                    <  SRR4302534.5232415/94‑1 (MQ=60)

                           GAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAG                                                                   >  SRR4302534.3496476/1‑94 (MQ=60)

                           GAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAG                                                                   <  SRR4302534.2120167/94‑1 (MQ=60)

                           GAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAG                                                                   <  SRR4302534.1578443/94‑1 (MQ=60)

                            AAACGTAATCCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGG                                                                  <  SRR4302534.1555614/94‑1 (MQ=60)

                            AAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGG                                                                  >  SRR4302534.3671264/1‑94 (MQ=60)

                            AAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGG                                                                  <  SRR4302534.2593072/94‑1 (MQ=60)

                            AAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGG                                                                  <  SRR4302534.1203160/94‑1 (MQ=60)

                            AAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGG                                                                  >  SRR4302534.2723277/1‑94 (MQ=60)

                             AACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGC                                                                 >  SRR4302534.5880399/1‑94 (MQ=60)

                             AACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGC                                                                 >  SRR4302534.4956447/1‑94 (MQ=60)

                               CGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGT                                                               >  SRR4302534.2795219/1‑94 (MQ=60)

                                GTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTT                                                              <  SRR4302534.1149956/94‑1 (MQ=60)

                                GTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTT                                                              <  SRR4302534.1408237/94‑1 (MQ=60)

                                 TAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTC                                                             >  SRR4302534.2295935/1‑94 (MQ=60)

                                 TAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTC                                                             >  SRR4302534.280136/1‑94 (MQ=60)

                                  AAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCA                                                            >  SRR4302534.5137388/1‑94 (MQ=60)

                                  AAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCA                                                            <  SRR4302534.6622735/94‑1 (MQ=60)

                                  AAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCA                                                            >  SRR4302534.306892/1‑94 (MQ=60)

                                   AACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAA                                                           >  SRR4302534.5969798/1‑94 (MQ=60)

                                    ACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAAC                                                          >  SRR4302534.5223828/1‑94 (MQ=60)

                                     CCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACG                                                         >  SRR4302534.2045613/1‑94 (MQ=60)

                                      CGACAATGCTGGTGACCGACCTGACGCTGCG‑TTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAA                                                       <  SRR4302534.1577847/94‑1 (MQ=60)

                                       GACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAA                                                       >  SRR4302534.5042169/1‑94 (MQ=60)

                                        ACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAG                                                      <  SRR4302534.3545375/94‑1 (MQ=60)

                                        ACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAG                                                      >  SRR4302534.851222/1‑94 (MQ=60)

                                         CAATGCTGGGGACCGACCTGACGCTGCGTTTTGATCCCGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGC                                                     >  SRR4302534.2164517/1‑94 (MQ=60)

                                          AATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCC                                                    >  SRR4302534.4868532/1‑94 (MQ=60)

                                            TGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTT                                                  >  SRR4302534.6438483/1‑94 (MQ=60)

                                            TGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTT                                                  <  SRR4302534.3779268/94‑1 (MQ=60)

                                             GCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTT                                                 <  SRR4302534.5720012/94‑1 (MQ=60)

                                              CTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTG                                                <  SRR4302534.3486023/94‑1 (MQ=60)

                                              CTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTG                                                <  SRR4302534.4106078/94‑1 (MQ=60)

                                                GGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCC                                              >  SRR4302534.2715879/1‑94 (MQ=60)

                                                GGTGACCGACCTGACGCTGCGTTCTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCC                                              <  SRR4302534.4180120/94‑1 (MQ=60)

                                                 GTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCC                                             <  SRR4302534.3974988/94‑1 (MQ=60)

                                                  TGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCG                                            >  SRR4302534.1345413/1‑94 (MQ=60)

                                                  TGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCG                                            >  SRR4302534.6533760/1‑94 (MQ=60)

                                                   GACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGT                                           >  SRR4302534.2767276/1‑94 (MQ=60)

                                                   GACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGT                                           >  SRR4302534.389961/1‑94 (MQ=60)

                                                    ACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTG                                          >  SRR4302534.4232377/1‑94 (MQ=60)

                                                    ACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTG                                          >  SRR4302534.1844971/1‑94 (MQ=60)

                                                      CGACCTGTCGTTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCC                                        <  SRR4302534.3343432/94‑1 (MQ=60)

                                                      CGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCC                                        >  SRR4302534.2617572/1‑94 (MQ=60)

                                                       GACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCT                                       <  SRR4302534.5122591/94‑1 (MQ=60)

                                                       GACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCT                                       >  SRR4302534.5461292/1‑94 (MQ=60)

                                                       GACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCT                                       >  SRR4302534.957429/1‑94 (MQ=60)

                                                       GACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCT                                       >  SRR4302534.738016/1‑94 (MQ=60)

                                                        ACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTG                                      >  SRR4302534.5100794/1‑94 (MQ=60)

                                                         CCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGG                                     <  SRR4302534.5182218/94‑1 (MQ=60)

                                                          CTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGT                                    >  SRR4302534.1872393/1‑94 (MQ=60)

                                                           TGACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTT                                   >  SRR4302534.2470370/1‑94 (MQ=60)

                                                           TGACGCTGCGTTTTGAGCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTT                                   <  SRR4302534.1744500/94‑1 (MQ=60)

                                                            GACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTC                                  <  SRR4302534.4304024/94‑1 (MQ=60)

                                                            GACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTC                                  <  SRR4302534.5405943/94‑1 (MQ=60)

                                                             ACGCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCA                                 <  SRR4302534.1577577/94‑1 (MQ=60)

                                                               GCTGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAA                               <  SRR4302534.233504/94‑1 (MQ=60)

                                                                 TGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACT                             >  SRR4302534.1798509/1‑94 (MQ=60)

                                                                 TGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACT                             <  SRR4302534.2774947/94‑1 (MQ=60)

                                                                 TGCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACT                             >  SRR4302534.3975701/1‑94 (MQ=60)

                                                                 TGCGTTTTGATCCTGAGTTCGAGAAGAACACTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACT                             <  SRR4302534.6520614/94‑1 (MQ=60)

                                                                  GCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTG                            <  SRR4302534.3376071/94‑1 (MQ=60)

                                                                  GCGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTG                            <  SRR4302534.5339015/94‑1 (MQ=60)

                                                                   CGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGA                           >  SRR4302534.387012/1‑94 (MQ=60)

                                                                   CGTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGA                           >  SRR4302534.1732167/1‑94 (MQ=60)

                                                                    GTTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGAC                          >  SRR4302534.5786542/1‑94 (MQ=60)

                                                                     TTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACG                         <  SRR4302534.304385/94‑1 (MQ=60)

                                                                     TTTTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACG                         <  SRR4302534.3009348/94‑1 (MQ=60)

                                                                       TTGATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCA                       <  SRR4302534.1592525/94‑1 (MQ=60)

                                                                          ATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAG                    >  SRR4302534.6335796/1‑94 (MQ=60)

                                                                          ATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAG                    <  SRR4302534.5112056/94‑1 (MQ=60)

                                                                          ATCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAG                    >  SRR4302534.1333292/1‑94 (MQ=60)

                                                                           TCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGG                   >  SRR4302534.6342089/1‑94 (MQ=60)

                                                                           TCCTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGG                   >  SRR4302534.4849915/1‑94 (MQ=60)

                                                                             CTGAGTTCGAGAAGAGCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGA                 <  SRR4302534.5468870/94‑1 (MQ=60)

                                                                             CTGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGA                 <  SRR4302534.2015441/94‑1 (MQ=60)

                                                                              TGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGAT                >  SRR4302534.349845/1‑94 (MQ=60)

                                                                              TGAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGAT                <  SRR4302534.117239/94‑1 (MQ=60)

                                                                               GAGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATA               >  SRR4302534.3638159/1‑94 (MQ=60)

                                                                                AGTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATAT              >  SRR4302534.997376/1‑94 (MQ=60)

                                                                                 GTTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATG             <  SRR4302534.5191916/94‑1 (MQ=60)

                                                                                  TTCGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGAGATGG            >  SRR4302534.220118/1‑94 (MQ=60)

                                                                                   TCGAGAAGACCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGG           <  SRR4302534.2025420/94‑1 (MQ=60)

                                                                                    CGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGG          >  SRR4302534.286598/1‑94 (MQ=60)

                                                                                    CGAGAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGG          >  SRR4302534.2537463/1‑94 (MQ=60)

                                                                                       GAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCG       >  SRR4302534.3628138/1‑94 (MQ=60)

                                                                                       GAAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCG       >  SRR4302534.4455651/1‑94 (MQ=60)

                                                                                        AAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGA      <  SRR4302534.2538938/94‑1 (MQ=60)

                                                                                        AAGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGA      >  SRR4302534.2539308/1‑94 (MQ=60)

                                                                                         AGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAA     <  SRR4302534.6038550/94‑1 (MQ=60)

                                                                                         AGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAA     <  SRR4302534.636232/94‑1 (MQ=60)

                                                                                         AGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAA     >  SRR4302534.112266/1‑94 (MQ=60)

                                                                                         AGAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGCATATGGGGCCGAA     <  SRR4302534.6352977/94‑1 (MQ=60)

                                                                                          GAACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAA    >  SRR4302534.4823138/1‑94 (MQ=60)

                                                                                           AACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAAT   >  SRR4302534.3557716/1‑94 (MQ=60)

                                                                                           AACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAAT   >  SRR4302534.831593/1‑94 (MQ=60)

                                                                                           AACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAAT   >  SRR4302534.2480181/1‑94 (MQ=60)

                                                                                            ACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATC  <  SRR4302534.4695140/94‑1 (MQ=60)

                                                                                            ACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATC  >  SRR4302534.6372421/1‑94 (MQ=60)

                                                                                            ACTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATC  <  SRR4302534.5962024/94‑1 (MQ=60)

TATCCCGGATCCGTTTGATCCGTCGAAGAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATC  >  NC_000913/4132488‑4132673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`