Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,632,259 T→C A207A (GCA→GCG)  rob ← right origin‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,632,2590TC100.0% 951.9 / NA 272A207A (GCA→GCG) robright origin‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (140/132);  total (140/132)

Only 100 of 278 total aligned reads displayed.

CTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGC  >  NC_000913/4632169‑4632349
                                                                                          |                                                                                          
cTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGc                                                                                          <  1:2421980/93‑1 (MQ=255)
  gCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCt                                                                                        <  1:3987504/93‑1 (MQ=255)
  gCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCt                                                                                        >  1:2823139/1‑93 (MQ=255)
      gCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATc                                                                                    >  2:1487088/1‑93 (MQ=255)
       ccGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATcc                                                                                   <  2:2721244/93‑1 (MQ=255)
        cGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCt                                                                                  >  2:5199798/1‑93 (MQ=255)
         ggTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTg                                                                                 >  2:5302959/1‑93 (MQ=255)
          gTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTgg                                                                                >  1:134148/1‑93 (MQ=255)
           ttCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTggg                                                                               >  1:4172783/1‑93 (MQ=255)
            tCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGc                                                                              <  1:944782/93‑1 (MQ=255)
              ccAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGTCTa                                                                            >  2:2745559/1‑93 (MQ=255)
                aGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAc                                                                          <  1:5857459/93‑1 (MQ=255)
                aGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAc                                                                          <  1:6184801/93‑1 (MQ=255)
                aGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAc                                                                          >  1:4015068/1‑93 (MQ=255)
                 gACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAcg                                                                         >  2:283712/1‑93 (MQ=255)
                 gACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAcg                                                                         >  2:2182879/1‑93 (MQ=255)
                   ccTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAcgcg                                                                       >  1:2404579/1‑93 (MQ=255)
                   ccTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAcgcg                                                                       >  2:2168296/1‑93 (MQ=255)
                   ccTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAcgcg                                                                       >  2:5555918/1‑93 (MQ=255)
                     ttCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggt                                                                     >  1:1478502/1‑93 (MQ=255)
                      tCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggtg                                                                    >  2:5654414/1‑93 (MQ=255)
                       cATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggtgg                                                                   <  2:5078344/93‑1 (MQ=255)
                       cATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggtgg                                                                   <  1:1099781/93‑1 (MQ=255)
                        aTAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggtggt                                                                  >  2:4230392/1‑93 (MQ=255)
                        aTAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggtggt                                                                  >  1:6048764/1‑93 (MQ=255)
                        aTAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCggtggt                                                                  <  1:4938433/93‑1 (MQ=255)
                            gTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAg                                                              >  1:1170991/1‑93 (MQ=255)
                            gTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAg                                                              >  1:279352/1‑93 (MQ=255)
                             tAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGa                                                             <  1:2374813/93‑1 (MQ=255)
                              aaaCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGaa                                                            <  2:614442/93‑1 (MQ=255)
                                aCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATa                                                          >  1:1722143/1‑93 (MQ=255)
                                aCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATa                                                          <  2:4929286/93‑1 (MQ=255)
                                aCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATa                                                          <  2:246980/93‑1 (MQ=255)
                                aCATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGAGGTATAGAATa                                                          >  1:3423910/1‑93 (MQ=255)
                                   tCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACct                                                       >  1:1575907/1‑93 (MQ=255)
                                     acaTATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACctct                                                     <  1:1857457/93‑1 (MQ=255)
                                     acaTATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACctct                                                     <  1:288209/93‑1 (MQ=255)
                                     acaTATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACctct                                                     >  1:2689216/1‑93 (MQ=255)
                                     acaTATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACctct                                                     <  1:4830691/93‑1 (MQ=255)
                                      caTATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCtt                                                    <  1:4495690/93‑1 (MQ=255)
                                        tatTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGc                                                  >  2:3150272/1‑93 (MQ=255)
                                        tatTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGc                                                  <  1:5948483/93‑1 (MQ=255)
                                        tatTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGc                                                  <  1:2274687/93‑1 (MQ=255)
                                          ttCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtc                                                >  2:3634757/1‑93 (MQ=255)
                                          ttCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtc                                                >  2:2055379/1‑93 (MQ=255)
                                          tccgccgcCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtc                                                <  1:754702/91‑1 (MQ=255)
                                             gccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtc                                             <  1:5443210/93‑1 (MQ=255)
                                             gccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtc                                             <  1:5649079/93‑1 (MQ=255)
                                             gccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtc                                             <  2:395370/93‑1 (MQ=255)
                                              ccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtcg                                            <  2:5865360/93‑1 (MQ=255)
                                              ccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtcg                                            >  2:2018737/1‑93 (MQ=255)
                                              ccgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCtcgtcg                                            <  1:4222055/93‑1 (MQ=255)
                                               cgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt                                           >  1:576310/1‑93 (MQ=255)
                                               cgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt                                           <  2:36680/93‑1 (MQ=255)
                                               cgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt                                           >  1:4635745/1‑93 (MQ=255)
                                               cgccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt                                           <  2:4855420/93‑1 (MQ=255)
                                                gccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCgtcgtc                                          >  1:6316477/1‑93 (MQ=255)
                                                gccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCgtcgtc                                          <  2:3341845/93‑1 (MQ=255)
                                                gccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCgtcgtc                                          <  1:5378150/93‑1 (MQ=255)
                                                gccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCgtcgtc                                          <  1:5104577/93‑1 (MQ=255)
                                                 cccTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCt                                         <  2:459269/93‑1 (MQ=255)
                                                 cccGGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCGGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCt                                         <  1:1218895/93‑1 (MQ=255)
                                                  ccTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCtt                                        <  1:4606748/93‑1 (MQ=255)
                                                  ccTGCAGCATCACCGGATGCCCCGTCAGTACATAGACATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCtt                                        >  1:4113709/1‑93 (MQ=255)
                                                   cTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCttt                                       <  2:2201547/93‑1 (MQ=255)
                                                    tGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTa                                      >  2:5425610/1‑93 (MQ=255)
                                                     gcagcaTCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTAt                                     <  2:255860/93‑1 (MQ=255)
                                                     gcagcaTCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTAt                                     >  2:2770419/1‑93 (MQ=255)
                                                      cagcaTCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATc                                    >  2:2680993/1‑93 (MQ=255)
                                                      cagcaTCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATc                                    >  1:2192447/1‑93 (MQ=255)
                                                       agcaTCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATcc                                   >  2:1268356/1‑93 (MQ=255)
                                                           tCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGAc                               <  1:467287/93‑1 (MQ=255)
                                                            cACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACt                              <  1:6415053/93‑1 (MQ=255)
                                                            cACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACt                              >  1:92631/1‑93 (MQ=255)
                                                            cACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACt                              <  2:3482718/93‑1 (MQ=255)
                                                            cACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACt                              >  2:6198512/1‑93 (MQ=255)
                                                             aCCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTc                             >  2:209132/1‑93 (MQ=255)
                                                               cGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCgg                           <  2:6262870/93‑1 (MQ=255)
                                                                ggATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGa                          <  1:5580355/93‑1 (MQ=255)
                                                                 gATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAc                         >  2:1177987/1‑93 (MQ=255)
                                                                 gATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAc                         >  1:2167716/1‑93 (MQ=255)
                                                                  aTGCCCCGCCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGCAcg                        >  2:4727015/1‑93 (MQ=255)
                                                                     ccccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGt                     >  2:952779/1‑93 (MQ=255)
                                                                     ccccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGt                     >  1:2554070/1‑93 (MQ=255)
                                                                     ccccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGt                     <  1:4971280/93‑1 (MQ=255)
                                                                       ccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGttt                   >  1:3700474/1‑93 (MQ=255)
                                                                       ccGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGttt                   >  1:3550207/1‑93 (MQ=255)
                                                                        cGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTttc                  >  1:5290116/1‑93 (MQ=255)
                                                                            aGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTttcattc              <  2:4540468/93‑1 (MQ=255)
                                                                                cATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGc          >  2:707649/1‑93 (MQ=255)
                                                                                 aTAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGcc         >  1:3068139/1‑93 (MQ=255)
                                                                                 aTAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGcc         <  1:5780900/93‑1 (MQ=255)
                                                                                  tAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCg        >  2:5935473/1‑93 (MQ=255)
                                                                                   atccATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCATGACGt       >  1:6016522/3‑93 (MQ=255)
                                                                                   aGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGt       >  1:3415443/1‑93 (MQ=255)
                                                                                    gCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTa      <  1:3819506/93‑1 (MQ=255)
                                                                                    gCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTa      >  1:1936994/1‑93 (MQ=255)
                                                                                    gCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTa      <  2:392454/93‑1 (MQ=255)
                                                                                      cATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTaga    >  2:4332839/1‑93 (MQ=255)
                                                                                        tCNGCCNGATCCTGGGCTAACNNGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGc  <  2:5803429/93‑1 (MQ=255)
                                                                                          |                                                                                          
CTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGC  >  NC_000913/4632169‑4632349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCAC  >  NC_000913/4632166‑4632351
                                                                                             |                                                                                            
CTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATTACATATTCTCCACCCTGCAGCATCACCCGATGCACCGTCAATACATAACCATCC                                                                                              >  SRR4302534.5861578/1‑94 (MQ=60)
  CCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGC                                                                                            <  SRR4302534.3110316/94‑1 (MQ=60)
   CTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCC                                                                                           <  SRR4302534.2486387/94‑1 (MQ=60)
    TGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCT                                                                                          >  SRR4302534.2897359/1‑94 (MQ=60)
     GCACGCCGGTTCCCAGCCCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATCCCCCGTCAGTACATAGCCATCCGCCTG                                                                                         <  SRR4302534.2312889/94‑1 (MQ=60)
     GCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTG                                                                                         <  SRR4302534.4094193/94‑1 (MQ=60)
      CACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGA                                                                                        <  SRR4302534.3014358/94‑1 (MQ=60)
        CGCCGGTTCCCAGACCTTCATAGGGAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATC                                                                                      >  SRR4302534.6554830/1‑94 (MQ=60)
         CCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCC                                                                                     >  SRR4302534.3513793/1‑94 (MQ=60)
          CCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCT                                                                                    <  SRR4302534.3509621/94‑1 (MQ=60)
            GGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGG                                                                                  >  SRR4302534.137678/1‑94 (MQ=60)
            GGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGG                                                                                  <  SRR4302534.2228630/94‑1 (MQ=60)
             GTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGG                                                                                 >  SRR4302534.4285497/1‑94 (MQ=60)
             GTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGG                                                                                 <  SRR4302534.2742135/94‑1 (MQ=60)
              TTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGC                                                                                >  SRR4302534.6163484/1‑94 (MQ=60)
              TTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGC                                                                                <  SRR4302534.4591353/94‑1 (MQ=60)
               TCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCT                                                                               <  SRR4302534.428694/94‑1 (MQ=60)
               TCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCT                                                                               <  SRR4302534.968816/94‑1 (MQ=60)
                 CCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAA                                                                             <  SRR4302534.4116293/94‑1 (MQ=60)
                  CAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAC                                                                            >  SRR4302534.2844850/1‑94 (MQ=60)
                  CAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAC                                                                            <  SRR4302534.5086019/94‑1 (MQ=60)
                  CAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAC                                                                            >  SRR4302534.4122665/1‑94 (MQ=60)
                  CAGACCTTCATAGGTAAACATCACATATTCCCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAAC                                                                            >  SRR4302534.1911136/1‑94 (MQ=60)
                   AGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACG                                                                           <  SRR4302534.6016496/94‑1 (MQ=60)
                   AGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACG                                                                           <  SRR4302534.6353136/94‑1 (MQ=60)
                     ACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCG                                                                         >  SRR4302534.2468484/1‑94 (MQ=60)
                       CTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGT                                                                       >  SRR4302534.6194695/1‑94 (MQ=60)
                       CTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGT                                                                       >  SRR4302534.1517377/1‑94 (MQ=60)
                        TTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTG                                                                      <  SRR4302534.2784665/94‑1 (MQ=60)
                        TTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTG                                                                      >  SRR4302534.6583942/1‑94 (MQ=60)
                         TCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGG                                                                     <  SRR4302534.2414031/94‑1 (MQ=60)
                         TCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGG                                                                     <  SRR4302534.667246/94‑1 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGT                                                                    <  SRR4302534.4282686/94‑1 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGT                                                                    <  SRR4302534.1128429/94‑1 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGT                                                                    <  SRR4302534.5164770/94‑1 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGT                                                                    >  SRR4302534.6213186/1‑94 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGT                                                                    <  SRR4302534.5944558/94‑1 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGT                                                                    <  SRR4302534.4846493/94‑1 (MQ=60)
                          CATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGT                                                                    >  SRR4302534.2573518/1‑94 (MQ=60)
                           ATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTA                                                                   <  SRR4302534.5072241/94‑1 (MQ=60)
                              GGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAG                                                                >  SRR4302534.286576/1‑94 (MQ=60)
                              GGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAG                                                                <  SRR4302534.637671/94‑1 (MQ=60)
                              GGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAG                                                                >  SRR4302534.1201769/1‑94 (MQ=60)
                              GGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAG                                                                <  SRR4302534.965596/94‑1 (MQ=60)
                                TAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAA                                                              >  SRR4302534.4876835/1‑94 (MQ=60)
                                TAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAA                                                              <  SRR4302534.2437880/94‑1 (MQ=60)
                                TAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAA                                                              >  SRR4302534.2165289/1‑94 (MQ=60)
                                 AAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAAT                                                             >  SRR4302534.5897102/1‑94 (MQ=60)
                                  AACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATA                                                            <  SRR4302534.5432593/94‑1 (MQ=60)
                                  AACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATA                                                            >  SRR4302534.1766889/1‑94 (MQ=60)
                                  AACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATA                                                            >  SRR4302534.5656063/1‑94 (MQ=60)
                                  AACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGAGGTATAGAATA                                                            >  SRR4302534.3515906/1‑94 (MQ=60)
                                   ACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATAC                                                           >  SRR4302534.5115909/1‑94 (MQ=60)
                                    CATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACC                                                          >  SRR4302534.185868/1‑94 (MQ=60)
                                    CATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACC                                                          >  SRR4302534.1813849/1‑94 (MQ=60)
                                     CTCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCT                                                         <  SRR4302534.5018891/94‑1 (MQ=60)
                                     ATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCT                                                         >  SRR4302534.1617140/1‑94 (MQ=60)
                                     ATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCT                                                         <  SRR4302534.5739737/94‑1 (MQ=60)
                                       CACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCT                                                       >  SRR4302534.2760284/1‑94 (MQ=60)
                                        CCATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTT                                                      <  SRR4302534.1262978/94‑1 (MQ=60)
                                        ACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGTATACCTCTT                                                      >  SRR4302534.5296268/1‑94 (MQ=60)
                                        ACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTT                                                      <  SRR4302534.2577591/94‑1 (MQ=60)
                                        ACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTT                                                      <  SRR4302534.1905804/94‑1 (MQ=60)
                                        ACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTT                                                      >  SRR4302534.4229827/1‑94 (MQ=60)
                                        ACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTT                                                      <  SRR4302534.295663/94‑1 (MQ=60)
                                        ACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTT                                                      <  SRR4302534.4961754/94‑1 (MQ=60)
                                         CATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATCCCTCTTG                                                     <  SRR4302534.5504770/94‑1 (MQ=60)
                                         CATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTG                                                     <  SRR4302534.4617361/94‑1 (MQ=60)
                                         CATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTG                                                     >  SRR4302534.4776319/1‑94 (MQ=60)
                                          ATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGC                                                    <  SRR4302534.2470570/94‑1 (MQ=60)
                                           TATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCT                                                   <  SRR4302534.2335031/94‑1 (MQ=60)
                                           TATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCT                                                   <  SRR4302534.6109991/94‑1 (MQ=60)
                                           TATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCT                                                   <  SRR4302534.6320640/94‑1 (MQ=60)
                                            ATTCGCCGCCCTGCAGCATCACTGGATGCCCCGTCGGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGGATAGAATACCTCTTGCTC                                                  <  SRR4302534.1453312/94‑1 (MQ=60)
                                            ATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTC                                                  >  SRR4302534.1692108/1‑94 (MQ=60)
                                            ATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTC                                                  >  SRR4302534.5466625/1‑94 (MQ=60)
                                            ATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTC                                                  >  SRR4302534.874080/1‑94 (MQ=60)
                                             TCCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCG                                                 <  SRR4302534.773407/94‑1 (MQ=60)
                                              TCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGT                                                >  SRR4302534.3595816/1‑94 (MQ=60)
                                               CGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTC                                               <  SRR4302534.5752009/94‑1 (MQ=60)
                                                GCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCG                                              <  SRR4302534.5801997/94‑1 (MQ=60)
                                                GCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCG                                              <  SRR4302534.5589837/94‑1 (MQ=60)
                                                 CCTCCCTGCAGCATCACCGGATGCCCTGTCAGTACATAGCCATCCGCCTGATCCTGGGTTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGT                                             <  SRR4302534.2381165/94‑1 (MQ=60)
                                                 CCGCCCTGCAGCATCCCCGGATTCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGT                                             <  SRR4302534.2882591/94‑1 (MQ=60)
                                                 CCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGT                                             <  SRR4302534.4335869/94‑1 (MQ=60)
                                                 CCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGT                                             >  SRR4302534.4761534/1‑94 (MQ=60)
                                                 CCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGT                                             >  SRR4302534.590455/1‑94 (MQ=60)
                                                  CGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTC                                            <  SRR4302534.3112521/94‑1 (MQ=60)
                                                  CGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTC                                            >  SRR4302534.6487188/1‑94 (MQ=60)
                                                  CGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTC                                            <  SRR4302534.5569222/94‑1 (MQ=60)
                                                   GCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCT                                           <  SRR4302534.5242338/94‑1 (MQ=60)
                                                   GCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCT                                           <  SRR4302534.244182/94‑1 (MQ=60)
                                                   GCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCT                                           <  SRR4302534.5523048/94‑1 (MQ=60)
                                                    CCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTT                                          <  SRR4302534.4298105/94‑1 (MQ=60)
                                                    CCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGACATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTT                                          >  SRR4302534.4224370/1‑94 (MQ=60)
                                                    CCCGGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCGGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTT                                          <  SRR4302534.1250982/94‑1 (MQ=60)
                                                     CCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTT                                         <  SRR4302534.4731756/94‑1 (MQ=60)
                                                     CCTGCAGCATCACCGGATGCCCAGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTT                                         <  SRR4302534.4377991/94‑1 (MQ=60)
                                                      CTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTA                                        <  SRR4302534.245676/94‑1 (MQ=60)
                                                        GCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATC                                      >  SRR4302534.2250415/1‑94 (MQ=60)
                                                         CAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCC                                     <  SRR4302534.1230398/94‑1 (MQ=60)
                                                         CAGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCC                                     >  SRR4302534.2055756/1‑94 (MQ=60)
                                                          AGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCT                                    <  SRR4302534.1204626/94‑1 (MQ=60)
                                                          AGCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCT                                    >  SRR4302534.1551608/1‑94 (MQ=60)
                                                           GCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTG                                   >  SRR4302534.5930965/1‑94 (MQ=60)
                                                           GCATCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTG                                   <  SRR4302534.2333841/94‑1 (MQ=60)
                                                             ATCCCCGGATGCGCCGTCAGTACATAGCCATCCGCCTGATACTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGAC                                 <  SRR4302534.3226792/94‑1 (MQ=60)
                                                              TCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACT                                >  SRR4302534.95104/1‑94 (MQ=60)
                                                              TCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACT                                <  SRR4302534.479072/94‑1 (MQ=60)
                                                              TCACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACT                                <  SRR4302534.5814067/94‑1 (MQ=60)
                                                               CACCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTC                               <  SRR4302534.6588131/94‑1 (MQ=60)
                                                                 CCGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGG                             <  SRR4302534.3672751/94‑1 (MQ=60)
                                                                  CGGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGA                            >  SRR4302534.6204299/1‑94 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                           <  SRR4302534.6241977/94‑1 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                           >  SRR4302534.2224968/1‑94 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                           >  SRR4302534.4695651/1‑94 (MQ=60)
                                                                   GGATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAC                           <  SRR4302534.5731120/94‑1 (MQ=60)
                                                                    GATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACG                          >  SRR4302534.5925062/1‑94 (MQ=60)
                                                                    GATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACA                          <  SRR4302534.1711248/94‑1 (MQ=60)
                                                                     ATGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATCCCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGC                         <  SRR4302534.1179803/94‑1 (MQ=60)
                                                                      TGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCG                        <  SRR4302534.6164714/94‑1 (MQ=60)
                                                                      TGCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCG                        >  SRR4302534.2897959/1‑94 (MQ=60)
                                                                       GCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGT                       >  SRR4302534.2621769/1‑94 (MQ=60)
                                                                       GCCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGT                       <  SRR4302534.2685415/94‑1 (MQ=60)
                                                                        CCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTT                      <  SRR4302534.723255/94‑1 (MQ=60)
                                                                        CCCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTT                      <  SRR4302534.5105869/94‑1 (MQ=60)
                                                                         CCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTT                     >  SRR4302534.1953380/1‑94 (MQ=60)
                                                                         CCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTT                     >  SRR4302534.3799951/1‑94 (MQ=60)
                                                                         CCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTT                     <  SRR4302534.14236/94‑1 (MQ=60)
                                                                         CCCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTT                     >  SRR4302534.3645674/1‑94 (MQ=60)
                                                                          CCGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTC                    >  SRR4302534.5432907/1‑94 (MQ=60)
                                                                           CGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCA                   <  SRR4302534.3941159/94‑1 (MQ=60)
                                                                           CGTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCA                   >  SRR4302534.1345995/1‑94 (MQ=60)
                                                                            GTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCAT                  >  SRR4302534.6101603/1‑94 (MQ=60)
                                                                            GTCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCAT                  >  SRR4302534.4145950/1‑94 (MQ=60)
                                                                             TCAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATT                 >  SRR4302534.4116150/1‑94 (MQ=60)
                                                                              CAGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTC                <  SRR4302534.3434443/94‑1 (MQ=60)
                                                                               AGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCA               >  SRR4302534.3675869/1‑94 (MQ=60)
                                                                               AGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCA               >  SRR4302534.391155/1‑94 (MQ=60)
                                                                               AGTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCA               <  SRR4302534.3994504/94‑1 (MQ=60)
                                                                                GTACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAG              <  SRR4302534.2895061/94‑1 (MQ=60)
                                                                                 TACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTTACTCGGACGCGTTTCATTCAGG             >  SRR4302534.876270/1‑94 (MQ=60)
                                                                                  ACATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGC            >  SRR4302534.4752506/1‑94 (MQ=60)
                                                                                   CATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCC           >  SRR4302534.3148721/1‑94 (MQ=60)
                                                                                    ATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCG          <  SRR4302534.4768507/94‑1 (MQ=60)
                                                                                    ATAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCG          <  SRR4302534.5937713/94‑1 (MQ=60)
                                                                                     TATCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCATGACGT         >  SRR4302534.6179995/1‑94 (MQ=60)
                                                                                     TAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGT         >  SRR4302534.3894418/1‑94 (MQ=60)
                                                                                     TAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGT         <  SRR4302534.5059962/94‑1 (MQ=60)
                                                                                     TAGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGT         >  SRR4302534.3507202/1‑94 (MQ=60)
                                                                                      AGCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTA        >  SRR4302534.1987638/1‑94 (MQ=60)
                                                                                       GCCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAG       <  SRR4302534.3922122/94‑1 (MQ=60)
                                                                                        CCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGA      <  SRR4302534.6076051/94‑1 (MQ=60)
                                                                                        CCATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGA      >  SRR4302534.4738351/1‑94 (MQ=60)
                                                                                         CATCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAG     >  SRR4302534.5617409/1‑94 (MQ=60)
                                                                                           TCCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCA   <  SRR4302534.6377793/94‑1 (MQ=60)
                                                                                            CCGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCAC  <  SRR4302534.5651596/94‑1 (MQ=60)
                                                                                             |                                                                                            
CTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCAC  >  NC_000913/4632166‑4632351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑