Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F13 I0 R1 206 15500.2 9478722 98.3% 9317583 135.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,712,763 A→C 19.8% A52A (GCT→GCG)  yceB ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,712,7630AC19.8% 136.9 / 4.1 88A52A (GCT→GCG) yceBlipoprotein
Reads supporting (aligned to +/- strand):  ref base A (57/10);  new base C (3/17);  total (60/28)
Fisher's exact test for biased strand distribution p-value = 1.25e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAA  >  NZ_CP022686/4712633‑4712902
                                                                                                                                   |                                                                                                                                           
gTTTCATGGTCGCTTTCAGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTATACGTTGAGGGTAACCTTATTCGTCTCTTCGCGTCCAATTT‑GGCTTGTAAGGTTTGTCATACCAATATGGGAGTCAGCCActc                                                                                                                                      >  6:709970/1‑136 (MQ=255)
 tttCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTTGGGTAACCTTTTTCGGCGCTTCGCGACCAAAGT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGGCAGGCACGCCgg                                                                                                                                   >  3:763559/1‑140 (MQ=255)
     aTGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGAGTCAGCCACGCCGGGTaa                                                                                                                               >  8:871438/1‑140 (MQ=255)
       ggTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTaa                                                                                                                               >  1:606777/1‑138 (MQ=255)
           gCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGTGTAACCTTATTCTGCTGTTCGCGACCAATTT‑TGCTGGTCAGGTTTGTCAGAACAATATGCGCGTCAGCCCCGCCCGGTAAACCGaa                                                                                                                         >  4:222109/1‑139 (MQ=255)
                   ccacccGAACTGGGAGTTCATGTCCAGTTTGGCTGCGCGGTTTTGGTTAACTTTATGGGGCGCTGCGC‑CCCAATTTGGGGTGGTTTGTTTTGACACAAAATTAGGGGCGCCCCCCACGCCGGGTAAACCGATATCttt                                                                                                                   <  6:166263/134‑1 (MQ=255)
                       ccGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGATTGTCAGAACAATATTGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                             >  8:58527/1‑140 (MQ=255)
                       ccGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGATCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCCGACTCGCCGGGGACACCGATATCTTTGGAGaa                                                                                                             >  4:415823/1‑140 (MQ=255)
                         gAACAGGGAGTTCATGACCAGATAGGCGTCTCCTGTTAGGCTAACCTTATTCGCCACTAAGCGACCAATAT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGAGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                             >  6:560843/1‑138 (MQ=255)
                                 aGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCGTATTCGGCTCTTCGCGACCAATTT‑GGCGGGTCCGGTTTGTCAGAACAATATGGGCGTCAGCCCCGCCGGGTAAACCGAAATATTTTGAGAAATtattat                                                                                                     >  2:136319/1‑138 (MQ=255)
                                  gTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGTTTTGTCAGAACAATATGGGCGTCCGCCCCGCCGGGGAAACCGATATCTTTTGAGAAATTATTATGtt                                                                                                  >  4:257997/1‑140 (MQ=255)
                                     cATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCg                                                                                               >  3:481933/1‑140 (MQ=255)
                                      aTGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGGAAACCGATATCTTTTGATAAATTATTATGttta                                                                                                >  6:723212/1‑137 (MQ=255)
                                         tCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCa                                                                                             >  1:989103/1‑138 (MQ=255)
                                         tCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAg                                                                                           >  8:982045/1‑140 (MQ=255)
                                             gATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAc                                                                                       >  3:1065055/1‑140 (MQ=255)
                                               ttGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGAACAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAATCCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAc                                                                                       >  6:376643/1‑138 (MQ=255)
                                                  gCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCCGCCACGCCGGGTAAACCGATAGCGTTTGAGAAAGTATTATGTTTCGCAAGCGCCTgg                                                                                    >  2:1046177/1‑138 (MQ=255)
                                                  gCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGtt                                                                                  >  4:1194036/1‑140 (MQ=255)
                                                  gCGTCGCGGGTTAGGTAACCCTTTTGCGGCGCTCCCCCACCATTTT‑GGCTGGGCTGTTTTGCCAAAACAATAGGGGCGCCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGtt                                                                                  <  4:354678/140‑1 (MQ=255)
                                                  gCGGCTCCGGTTAGGTAAACATTATTCGCCTCTTCGAGACAAATTT‑GGCTGGTCTGGTTTGTCATAAAAATAGGGGCGTCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTgg                                                                                    <  6:753184/138‑1 (MQ=255)
                                                    gTCTCCGGTGAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGATGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCCCGCCGGGGAAACCGATATCTTTTGAGAAATTATTATGTTGCGCAAGCGCCTGGtt                                                                                  >  2:834427/1‑138 (MQ=255)
                                                     tctcCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑TGCTGGTCATGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGTGTAAACCGATATCTTTAGAGAAAGTATTATGTATCGCAATCGACTGGGTatt                                                                               >  8:668977/1‑138 (MQ=255)
                                                     gggcgggTTTGGGAAACATTATTCGCCGCTTCGCTCCCAATTG‑GCCGGGGCGGGTTTGTCACAACAATATGGGCGGCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAt                                                                               <  4:873535/135‑1 (MQ=255)
                                                         cGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGATTGGTTAAttt                                                                             >  5:945293/1‑138 (MQ=255)
                                                              aGGGTAACCTTATTCGGCTCTTCGCGCCCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTCGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTc                                                                      >  4:802533/1‑140 (MQ=255)
                                                                ggTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                                                    >  7:217723/1‑140 (MQ=255)
                                                                 gTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGt                                                                   >  8:258715/1‑140 (MQ=255)
                                                                  aacccTTATTCGGCGCTTCGCTACCAATTG‑GGGTGTCAATGTTTGTCACAAAATTATGGGCTGAAGCCACGCCGGGTAAACCGATATCTTTTGAGAACTTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                                                    <  6:1050742/135‑1 (MQ=255)
                                                                   aaCCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGt                                                                   >  1:942261/1‑138 (MQ=255)
                                                                    actttATTGGGGTTTTCCCTACCATTTG‑GGGTGGTCTGGTTTGTAAAAAAAATTTGGGCGGCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                                <  8:1001705/137‑1 (MQ=255)
                                                                     ccTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTAGGTGATg                                                               >  3:594959/1‑140 (MQ=255)
                                                                     cattATTCGCTCCTCCGAGACAATTTG‑GGCTGGTCTGGTTGGACACAAAAATTTGGGGGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGa                                                                 <  6:215076/136‑1 (MQ=255)
                                                                      tttATTCGGCTCTTCGCTCCCAATTG‑GGCGGGTCTGGTTTGACACAAAAATTTGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATgg                                                              <  8:354059/139‑1 (MQ=255)
                                                                      cTTATTCGGCTCTGCGCTACCAATTG‑GGCGGGTCAGGTTTGTCATAACAATTTGGGCGCAAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATgg                                                              <  4:1117009/140‑1 (MQ=255)
                                                                       ttATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg                                                               >  6:1034060/1‑138 (MQ=255)
                                                                          ttCGGCTCTTCTCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                          >  7:381609/1‑140 (MQ=255)
                                                                          ttCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgtgtt                                                          >  7:820140/1‑139 (MQ=255)
                                                                          ttCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                          >  8:455216/1‑140 (MQ=255)
                                                                          ttCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                          >  8:336664/1‑140 (MQ=255)
                                                                          ttCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                          >  8:1126715/1‑140 (MQ=255)
                                                                          ttCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                          >  8:889794/1‑140 (MQ=255)
                                                                           tcgcgtctTCGCTACCTTTTT‑GGTGGGTCGGGTTTGAAATAAATTTAGGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTAt                                                         <  4:225757/135‑1 (MQ=255)
                                                                            cGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTACACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAGTGGTTAATTTCTTGTTCGGTGATGgtgtt                                                          >  2:1130412/1‑137 (MQ=255)
                                                                            cGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATTGTGTa                                                          >  2:497727/1‑138 (MQ=255)
                                                                            cGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgtgtt                                                          >  6:361852/1‑137 (MQ=255)
                                                                               ctctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTTAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  3:890392/1‑140 (MQ=255)
                                                                               ctctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTTTTCGGTGATGGTGTATTggg                                                     >  8:5104/1‑140 (MQ=255)
                                                                               ctctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  3:102093/1‑140 (MQ=255)
                                                                               ctctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  8:682964/1‑140 (MQ=255)
                                                                                tctGCGCCACAAATTT‑GGCGGGTCAGGTTTGTAAAAAAAATAGGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGt                                                    <  7:288686/140‑1 (MQ=255)
                                                                                 ctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCCCGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  6:217416/1‑138 (MQ=255)
                                                                                 ctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  1:31329/1‑138 (MQ=255)
                                                                                 ctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  1:101963/1‑138 (MQ=255)
                                                                                 ctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  2:33517/1‑138 (MQ=255)
                                                                                  ttCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGa                                                  >  8:1056755/1‑140 (MQ=255)
                                                                                   tCGCGACATATTT‑TTCTGGACATGTTTGTCATAAAATTATGGGCTGCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAg                                                 <  4:617216/140‑1 (MQ=255)
                                                                                   ccgccaacAATGT‑GGCTGCTCCGGTTTGACAGAACAAAATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATTGTGTATTGGGTGAg                                                 <  4:267980/133‑1 (MQ=255)
                                                                                   cccccaCCAATTG‑GTCGGGTCTGTTTTGTACCAACAATAGGGGCTTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTg                                                   <  2:352439/133‑1 (MQ=255)
                                                                                    cgcgACCAATTT‑GGCTGGTCAGGTTTGTCAGTACAATATGGGCGTCAGCCACGCCTGGTAAACCGATATCTTATGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggggtc                                                  >  6:1049843/1‑135 (MQ=255)
                                                                                      cgACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGAGATGGTGGATTGGGTGAGt                                                >  2:891683/1‑138 (MQ=255)
                                                                                        aacacttt‑GGCTGGTCCGGTTTGTCAGAACAATCTGGGCTTTCGCCTCGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGGTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGGGAGTTGAt                                            <  4:852771/135‑1 (MQ=255)
                                                                                                 ggCGGGTCTGGTTTGTCACAAAAATCGGGGCGCCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCa                                    <  7:568660/140‑1 (MQ=255)
                                                                                                      gaccggTTTTCCATAACAATATGGGCTGCTGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               <  4:83180/136‑1 (MQ=255)
                                                                                                      gTCTGGTTTGTCACAAAAATAGGGCGGTCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACg                                 <  6:196566/138‑1 (MQ=255)
                                                                                                      gTCTGGTTTGACACAAAATTGTGGGGGGCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               <  3:207751/140‑1 (MQ=255)
                                                                                                      gTCATGTTTGTCACCACAATTGGGGCGTCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               <  8:718819/140‑1 (MQ=255)
                                                                                                      gTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               >  7:874963/1‑140 (MQ=255)
                                                                                                      gTCAGGTTTGTCAGAAAAATAGGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACg                                 <  6:61038/138‑1 (MQ=255)
                                                                                                        cAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                  >  1:965736/1‑103 (MQ=255)
                                                                                                        cAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                  <  2:965736/103‑1 (MQ=255)
                                                                                                        cAGGTTTGTCAGAACAATATGGGCGTCAGCAACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCATGCGACTGGTTAAGTTCTTGTTCGGTGATGTAGTCTTGGGTGAGTTGATTACAGCCAACGAGCa                             >  7:349276/1‑140 (MQ=255)
                                                                                                                tCATAACAATAGGGGCGGCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                     <  8:294578/140‑1 (MQ=255)
                                                                                                                gcAGAACATACTGGGCGCCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCAc                       <  6:71716/137‑1 (MQ=255)
                                                                                                                cccaaaCAATCTGGTGCTCACCCACGCCGCGTAAACCGATAGCTTTTGAGAAATTATTATGTTTCGCAAGGGACTTGTTAATTTCTTGTTCGGTGATTGTGTATTGGGTGAGTTGATTACAGCCAACTAGCAGGCCAc                       <  2:930207/134‑1 (MQ=255)
                                                                                                                ccAGAACAATTTGGGCGGCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                     <  4:323213/139‑1 (MQ=255)
                                                                                                                     aCAATCTGGGGGTCCGCCACGCCGGGTAATCCGATCTCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGGGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACg                  <  2:747597/138‑1 (MQ=255)
                                                                                                                         tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGCCGATCAAt            >  7:640040/1‑140 (MQ=255)
                                                                                                                         tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt            >  4:465507/1‑140 (MQ=255)
                                                                                                                         tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt            >  8:506050/1‑140 (MQ=255)
                                                                                                                         tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTAAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt            >  7:477918/1‑140 (MQ=255)
                                                                                                                           tggtcctCAGCAACCCCGGGTAAACCGATATCTATTGAGAAATAATTATGATTCGAAAGAGACAGGTTAAATACTTCTTCAGTCATGCTCTAATGTGTCAGTTGATTACACCCAACGAGCAGTCCACAGACGATAAAt            >  2:1041487/7‑138 (MQ=255)
                                                                                                                           tGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt            >  6:8978/1‑138 (MQ=255)
                                                                                                                           tGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt            >  6:825463/1‑138 (MQ=255)
                                                                                                                                ggcAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGc       <  1:369982/136‑1 (MQ=255)
                                                                                                                                gTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGc       >  6:734509/1‑138 (MQ=255)
                                                                                                                                gTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGc       >  1:814327/1‑138 (MQ=255)
                                                                                                                                 tCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  7:618730/1‑140 (MQ=255)
                                                                                                                                   aGCCACGCCGGGTAATCCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGCCGATCAATGCAGCGGc    >  6:564774/1‑138 (MQ=255)
                                                                                                                                   aGCCACGCCGGGTAAGCCGATATCTTTGGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGGTCGGTGATGGTGTATTGGGTGAGTTGAATACCGCCAACGAGCAGGCCACTGACGATCAATGCAGccgg    >  5:883432/1‑135 (MQ=255)
                                                                                                                                   aGCCACGCCGGGTAAACCGATATCTTTTGAGAATTTATTATGTTTCGCACTCGACTGTATAATTTCTAGTACGGTGATGGAGTATTGGGTGAGGTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaa  >  7:827893/1‑140 (MQ=255)
                                                                                                                                   aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  5:522155/1‑138 (MQ=255)
                                                                                                                                   aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  6:898372/1‑138 (MQ=255)
                                                                                                                                   aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  5:1133828/1‑138 (MQ=255)
                                                                                                                                   |                                                                                                                                           
GTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAA  >  NZ_CP022686/4712633‑4712902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A