Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F35 I0 R1 275 15706.7 10805118 98.3% 10621430 134.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,712,763 A→C 20.0% A52A (GCT→GCG)  yceB ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,712,7630AC20.0% 183.7 / 9.1 118A52A (GCT→GCG) yceBlipoprotein
Reads supporting (aligned to +/- strand):  ref base A (78/12);  new base C (3/24);  total (81/37)
Fisher's exact test for biased strand distribution p-value = 4.43e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGC  >  NZ_CP022686/4712625‑4712900
                                                                                                                                             |                                                                                                                                         
cAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAg                                                                                                                                          >  7:1055006/1‑140 (MQ=255)
      cAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGCCCCATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TCGGCCTCAGCCAc                                                                                                                                      >  2:100654/1‑138 (MQ=255)
      cAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCCTCCAc                                                                                                                                      >  2:100658/1‑138 (MQ=255)
        gTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTAGGCTCTTCGCGCCCAATTT‑TGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGc                                                                                                                                    >  1:313864/1‑138 (MQ=255)
         tttCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCGTCGCGACCAATTT‑TGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGAGGCATCCACGcc                                                                                                                                   >  4:37453/1‑138 (MQ=255)
                     tttCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑TGCTGGTCAGGTTTGTCAGAAACAT‑A‑TTGGCGTCAGCCACGCCGGGTAAACCGat                                                                                                                       >  1:499192/1‑138 (MQ=255)
                             gaCCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGGCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTg                                                                                                               >  4:1181438/2‑138 (MQ=255)
                               ccGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                           >  7:244570/1‑140 (MQ=255)
                                 gAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAATCCGATATCTTTTGAGaa                                                                                                           >  3:868210/1‑138 (MQ=255)
                                 gAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                           >  2:1384976/1‑138 (MQ=255)
                                 gAACAGGGAGTTCATGTCCAGATTGGCGTCACCGGTAAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGCTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACCCCGGGTAAACCGATATCTTTTGAGaa                                                                                                           >  3:164270/1‑138 (MQ=255)
                                 gAACAGGGAGTTCATGTACATATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGCCCAAATT‑GGCGGGTCAGGTTTGTCTGAACAAT‑A‑TGGGCGTCAGCCCCGCCGGGTAAACGGATATATTTTGAGaa                                                                                                           >  5:241798/1‑138 (MQ=255)
                                     aGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTAAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAatta                                                                                                       >  5:163629/1‑138 (MQ=255)
                                         aGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATtattat                                                                                                   >  4:402926/1‑138 (MQ=255)
                                            tCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGtt                                                                                                >  4:711428/1‑138 (MQ=255)
                                             cATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCg                                                                                             >  7:68692/1‑140 (MQ=255)
                                             cATGTCCAGATTGGCGTCTCCGGTTAGGGTAACATTATTCGGCTCTTCGCGACCCATTT‑TGCTGGTCAGGTTTGTCAGAACAAT‑A‑TTGGCGGCAGCCACGCCGGGTAAACCGATATCGTTTGAGAATGTATTATGTTTCg                                                                                             >  7:1259594/1‑140 (MQ=255)
                                              aTGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGttta                                                                                              >  5:225823/1‑137 (MQ=255)
                                               tGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGAGATCTTTTGAGAAATTATTATGTTTCg                                                                                             >  3:794290/1‑138 (MQ=255)
                                                 tCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCa                                                                                           >  1:18320/1‑138 (MQ=255)
                                                    ctttgggCGACCCCGTTTTGGTTAACCTTATTCGGCTCTTCCCCACAAATTT‑GGGTGTTCTGGTTTGTCACAAAAAT‑A‑GGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGc                                                                                        <  2:173794/133‑1 (MQ=255)
                                                     gATTTGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTGAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATGATGTTTCGCAAGCGAc                                                                                     >  8:1195910/1‑140 (MQ=255)
                                                     gATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCCCGCCGGGTAAACCGATATATTTTGAGAAATTATTATGTTTCGCAAGCg                                                                                       >  4:514143/1‑138 (MQ=255)
                                                     gATTGGCGTCTCAGGTTAGGGTAAACTTATACTGCTCTTCGAGAAAAATTT‑TGGTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTACACCGATATCTTATGAGAAATTATTATCTTTAGCAAGCGAc                                                                                     >  8:774831/1‑140 (MQ=255)
                                                       ttGGCGTCTCCGGTTAGGGTACCCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTTAGAAATTATTATGTTTCGCAAGAGAc                                                                                     >  4:101161/1‑138 (MQ=255)
                                                             tctcCGGTTAGGGTAACCTTATTCGGCTCTTCGCGCCCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTGTTGAGAAATTATTATGTTTCGCAAGCGACGGGGTa                                                                               >  3:1280375/1‑138 (MQ=255)
                                                                ggtgTTAGTTTAACTTTATTCGCTACTGCGCGACCTTTTT‑GGTTGGTGTGGTTTGTCATAAAATT‑A‑TGGGGGTCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAtt                                                                            <  2:1373078/135‑1 (MQ=255)
                                                                ccGGTTAGGGTAACCTTATTCGGAGCTTCGCGACCAATTT‑GGCTGGTCTGGTTCGGCAGAACAAT‑T‑GGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAtt                                                                            <  4:1015728/138‑1 (MQ=255)
                                                                 cGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAttt                                                                           >  4:15635/1‑138 (MQ=255)
                                                                   ttttgggTAACTTTTTTCGGCTCTTCGCGACATTTTT‑GGGTGGTCTGTTTTGTCATAAAAAT‑T‑TGGGCGCCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCt                                                                         <  1:1117474/134‑1 (MQ=255)
                                                                    ttAGGGTAACCTTATTCGGCTCTTCGCGACAAATTT‑GGCTGGTCAGGTTAGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGt                                                                      >  8:1225057/1‑140 (MQ=255)
                                                                     ttggttAACATTATTCGGCTCTGCGCCACCATTTG‑GGCGGGGCTGGTTTGTCACAAAAAT‑T‑GGGGCGCCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTg                                                                       <  2:453478/133‑1 (MQ=255)
                                                                        ggTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                                                  >  7:286282/1‑140 (MQ=255)
                                                                          tAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                                                  >  1:266392/1‑138 (MQ=255)
                                                                          tAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTATTGTTCgg                                                                  >  5:396339/1‑138 (MQ=255)
                                                                          aacctttATTCGGCTCTGCGCTAATATTTT‑GTTTGGTCAGTTTTTTCA‑TACAATAA‑TGTGCGTCATCCACGCCGGGTACACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                <  8:188018/135‑1 (MQ=255)
                                                                          aaaacTTATTCGACTCTTCGCAACCATTTT‑GGCGGGCCAGTTTGTTCCCAACAAA‑A‑TGGGCTACCCCCACGCCGGGGAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                <  8:188020/136‑1 (MQ=255)
                                                                            attttATTCGTCTTTTCGCGACAATTTG‑GGCTGTTCTGTTTTGCCACAAAATT‑T‑GGGGCGCACCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCTCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                <  3:514143/135‑1 (MQ=255)
                                                                            acttttttCGGAACTTCGCCACAATTTT‑GGGGGGTCTGGTTTGTCATAAAAAT‑T‑TGGGCGCCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                <  4:469241/132‑1 (MQ=255)
                                                                            actttATTCGGCTATTGGCTACAATTTT‑GGGGGGTTTTGTTTTTATTAAAAAT‑T‑GGGGCGTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                <  1:1284448/135‑1 (MQ=255)
                                                                            aacTTTTTGGGCTCTTCGCCACAATTTT‑GGCTGGCCAGGTTTGTCATAAAAAT‑T‑TGGGCGTCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                <  2:411307/136‑1 (MQ=255)
                                                                            aCCTTATTCGGCTCTTCCCGCCAAATTT‑GGCGGGTCCGGTTTGAAATAAAAAA‑T‑TGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                              <  8:156775/140‑1 (MQ=255)
                                                                             ccTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg                                                             >  8:1147049/1‑140 (MQ=255)
                                                                             aattATTCGGCTCTACGCCAAAATTTG‑GGCTGGTCTGTTTGGACTCAACAAT‑T‑GGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGa                                                               <  6:92083/136‑1 (MQ=255)
                                                                              tttATGCGGCTTTTCGCTACCATTTT‑GGGTGGTCTGGTTTGCCATAAATTT‑T‑GGGGCGGCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                              <  5:383735/137‑1 (MQ=255)
                                                                              cTTATTCGGCTCTTCGCGCCCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                              >  3:860244/1‑138 (MQ=255)
                                                                              cTTATGCGGCTCTCCGCTCCTAATTT‑GCGTGGTCCGGTTTCCCAGAACAAT‑G‑GGGCCCTACGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                              <  2:120663/138‑1 (MQ=255)
                                                                                  ttCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                        >  8:612861/1‑140 (MQ=255)
                                                                                    cGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGACCAAT‑A‑TGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGAAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                        >  6:102364/1‑138 (MQ=255)
                                                                                    cGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                        >  1:104260/1‑138 (MQ=255)
                                                                                    cGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGCGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAAGGTGTa                                                        >  5:197601/1‑138 (MQ=255)
                                                                                    cGGCTCTTCGCGACCAATTT‑GGCTGGTAAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAAGGTGTa                                                        >  3:368529/1‑138 (MQ=255)
                                                                                    cGGCTCTTCGCGAACAATTT‑GGCTGGTCATGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGCTATCGTTTGAGCATTTTTTATGTTTCGAAAGCGACTGGGTAAGTTCGTGTTCGGTGAGGgtgtg                                                        >  4:128882/1‑137 (MQ=255)
                                                                                       ctctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCGCGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                   >  7:913978/1‑140 (MQ=255)
                                                                                       ctctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                   >  7:1253444/1‑140 (MQ=255)
                                                                                       ctctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                   >  7:244546/1‑140 (MQ=255)
                                                                                       ctctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                   >  7:557698/1‑140 (MQ=255)
                                                                                       ctctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                   >  8:734089/1‑140 (MQ=255)
                                                                                       ctctTCGCGACCAATTT‑GGCTGGTCAGGTATGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCACGGTCAACCGATATCGTTTGAGAAAATATTATGTTTCGCAAGCGACTTGATAATTTCTTGTTCGGTGATGGTGTATTggg                                                   >  7:175830/1‑140 (MQ=255)
                                                                                        tctTCGCGACAAATTG‑GGCGGGGCTGGTTTTTCACAAAAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTgg                                                    <  4:561090/138‑1 (MQ=255)
                                                                                        cctgcgcgACAATTTT‑GGCTGGAGAGGTTTGT‑ATAACAAT‑ACTGGGCCTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTgg                                                    <  1:1113827/134‑1 (MQ=255)
                                                                                         ctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                   >  4:901739/1‑138 (MQ=255)
                                                                                          ttCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGa                                                >  8:543822/1‑140 (MQ=255)
                                                                                          ttCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTGAATTTCTTGTTCGGTGATGGTGTATTGGGt                                                  >  3:1282861/1‑138 (MQ=255)
                                                                                           gcgcgACAAATTT‑GGCGGGTCAGGTTTGACATAAAAAT‑A‑TGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTg                                                 <  6:844296/137‑1 (MQ=255)
                                                                                           gcgccACAAATTG‑GGCGGGTCTGGTTTGCCATAAAAAT‑T‑GGGGCGGCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTg                                                 <  6:633231/137‑1 (MQ=255)
                                                                                           ccgctACCATTTG‑GGCGGGTCAGTTTTGCAATAAAAAT‑A‑TGGGGGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTg                                                 <  6:959375/137‑1 (MQ=255)
                                                                                            gcgctacAATTTGGGCTGGTCTGGTTTTTAACAAAAAT‑T‑GGGGGGCCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAg                                               <  7:1100888/134‑1 (MQ=255)
                                                                                            cgcgACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAATCCGATATCTTTAGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGa                                                >  4:865778/1‑138 (MQ=255)
                                                                                            cgcgACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGa                                                >  1:1325986/1‑138 (MQ=255)
                                                                                            cgcgACAAATAT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGTGTGa                                                >  4:661939/1‑138 (MQ=255)
                                                                                                   atttt‑GGCTGGTCTGTTTTGTCATAAAAAT‑T‑TGGGCGGCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGAtt                                         <  2:104260/136‑1 (MQ=255)
                                                                                                   aaTTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGAtt                                         >  2:329489/1‑138 (MQ=255)
                                                                                                              gtgaGGTTTGCCACAACAAT‑A‑GGGCCGACAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTATCGCAAGCGACTGGTTAATTTCTTGTTCGTTGATGGTGTATTGGGTGAGTTGATTACAGCCAACg                               <  6:687961/135‑1 (MQ=255)
                                                                                                              gtctgttttGACAGAAAAAT‑T‑GGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACg                               <  4:765478/132‑1 (MQ=255)
                                                                                                              gatcggTTTTTCCTAAAAAT‑A‑TGGGGGCCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACg                               <  6:253462/134‑1 (MQ=255)
                                                                                                              gTCCGGTGTGTCACAACAAT‑A‑TGGGCGCCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACg                               <  6:849289/138‑1 (MQ=255)
                                                                                                                cAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGac                             >  2:1397505/1‑137 (MQ=255)
                                                                                                                          ataaaaaT‑A‑TGGGCTGCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGAc                 <  8:612917/135‑1 (MQ=255)
                                                                                                                          aGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGTGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                   >  4:1145605/1‑138 (MQ=255)
                                                                                                                          aGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                   >  4:1026127/1‑138 (MQ=255)
                                                                                                                           aaaCAAT‑T‑TGGGCGTCCGCCACGCCGTGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACg                <  8:1020945/139‑1 (MQ=255)
                                                                                                                             aCAAT‑A‑TGGGCGTCTGCCAGGCCGGGTAAACCGATATCTTTTGAGAAATTATTATTTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACg                <  5:1191584/138‑1 (MQ=255)
                                                                                                                             aCAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAATGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGAt              >  7:210789/1‑140 (MQ=255)
                                                                                                                              aaaaaa‑tGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACg                <  6:1208321/133‑1 (MQ=255)
                                                                                                                               aaT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGAt              >  2:677224/1‑138 (MQ=255)
                                                                                                                               aaT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGAt              >  1:900672/1‑138 (MQ=255)
                                                                                                                               aaT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATAACAGCCAACGAGCAGGCCACTGCCGAt              >  1:557129/1‑138 (MQ=255)
                                                                                                                                 t‑a‑tGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTGGTTCGGTGATGGTGTATTGGGTGAGGTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt          >  8:172150/1‑140 (MQ=255)
                                                                                                                                   a‑tGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCaa           >  1:976485/1‑138 (MQ=255)
                                                                                                                                     tGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCGAACGAGCAGGACACTGACGATCAAt          >  4:1295607/1‑138 (MQ=255)
                                                                                                                                     tGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt          >  2:4904/1‑138 (MQ=255)
                                                                                                                                     tGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt          >  4:328214/1‑138 (MQ=255)
                                                                                                                                     tGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt          >  2:465284/1‑138 (MQ=255)
                                                                                                                                        gCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGc     >  7:1063552/1‑140 (MQ=255)
                                                                                                                                          gTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGCCTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGCGCAGGCCACTGACGATCAATGCAGc     >  6:1049303/1‑138 (MQ=255)
                                                                                                                                          gTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGc     >  5:1019597/1‑138 (MQ=255)
                                                                                                                                            cAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACGGACGATCAATGGAGCgg   >  4:737381/1‑138 (MQ=255)
                                                                                                                                            cAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTCGATAATTTCTTGTTCGGTGATGGAGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCgg   >  4:660645/1‑138 (MQ=255)
                                                                                                                                             aGCCACGCCTGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTAGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGCTCAATGCAGCGGc  >  1:655219/1‑138 (MQ=255)
                                                                                                                                             |                                                                                                                                         
CAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAAT‑A‑TGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGC  >  NZ_CP022686/4712625‑4712900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A