Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F36 I0 R1 229 12610.8 9774876 98.4% 9618477 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,712,763 A→C 18.1% A52A (GCT→GCG)  yceB ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,712,7630AC18.1% 171.1 / 10.0 106A52A (GCT→GCG) yceBlipoprotein
Reads supporting (aligned to +/- strand):  ref base A (71/11);  new base C (2/19);  total (74/32)
Fisher's exact test for biased strand distribution p-value = 3.57e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

TCAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAA  >  NZ_CP022686/4712624‑4712902
                                                                                                                                               |                                                                                                                                           
tCAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAAAAGGGAGTTCATGTCCAGCTTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCa                                                                                                                                             >  3:1210101/1‑140 (MQ=255)
       cattttCATTGTCTCTTTCTGGCTACCGAACAGGGAGTTCATGTACAAATTGGAGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTAGAGCACAAATT‑TTC‑TGGTCAGTATTGTC‑AGAACAATATGGGCGTCAGCAACGc                                                                                                                                      >  4:524181/4‑140 (MQ=255)
       cAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTAATGTCCAGATTGGCGTCTCCGGTTAGGGTAAAC‑TTATTCGCCTCTTCGAGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCAAGc                                                                                                                                      >  4:165568/1‑140 (MQ=255)
        aGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGcc                                                                                                                                     >  4:38693/1‑140 (MQ=255)
          tttCAGGGGCGCTTTCTGGCTCCCCAACTGGGATTTCTTGCCCAGATTGGCGTCGCGGGTTCGGTTAACC‑TTATTCGGCTCTTCGCCACCATTTT‑GGC‑GGGTCTGTTTTGCC‑ACAACAATAGGGGCGCCCCCCACGCCgg                                                                                                                                   <  4:561775/140‑1 (MQ=255)
                    gCTTTCTGGCTACCGAACAGGGAGTTCATGTACAGATTTGCGTCTCCGGTTAGGGTAACC‑TTATTAGTCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAAAAAGGGCGTCAGCCCCGCCGGGTAAACCgct                                                                                                                         >  3:1239806/1‑138 (MQ=255)
                               aCCTAATAGTGAGTTGTTGTCCCGTTTGGCGTCTGTGGTTTGGTAAACC‑TTATTCGGCTGTTCGCTACCAATTT‑GGC‑TGGTATGGTTTGTC‑ATAAAAATTTGGGCGTCACCCACGCCGGGTAAACCGATATCTTTTgaga                                                                                                              <  8:392066/140‑1 (MQ=255)
                                ccGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGATTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                             >  6:1050060/1‑140 (MQ=255)
                                ccGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATACGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                             >  5:754263/1‑140 (MQ=255)
                                ccGAACAGGGAGTTCATGTCCAGATTGGAGTCTCCGGGTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATGT‑GGC‑GGGGCAGGTTTGTC‑CGTACAATATGGGCGTCAGCCACGCCGGGTTAACCGAAATCGTTTGAGaa                                                                                                             >  6:655919/1‑140 (MQ=255)
                                ccGAACAGGGAGTTCATGTACAGATTGGCGACTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAAATT‑GGC‑GGGTCAGGTATCTC‑AGAACAATATCGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                             >  6:1054608/1‑140 (MQ=255)
                                        ggAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGGTTGTC‑AGAACAATATGGTCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATtattat                                                                                                     >  3:1070706/1‑140 (MQ=255)
                                          aGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGGC‑AGAACAATATGGGCGGCAGCCACGCCGGGTCAACCGCTATCTTTTGAGAAATGATTATGt                                                                                                   >  3:73350/1‑140 (MQ=255)
                                            ttCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGCCCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAAATATTATGttt                                                                                                 >  8:819468/1‑140 (MQ=255)
                                             tCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCCCGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGttta                                                                                                >  3:333313/1‑139 (MQ=255)
                                             tCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGtt                                                                                                  >  2:370839/1‑138 (MQ=255)
                                             tCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTc                                                                                                >  3:196888/1‑140 (MQ=255)
                                             tCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTA‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGttta                                                                                                >  5:284404/1‑139 (MQ=255)
                                             tCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGAGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCTTCAGCCACGCCGGGGAAACCGATATCTTTTGAGAAATTATTATggtac                                                                                                >  6:702206/1‑136 (MQ=255)
                                              cATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTAGCGACCAATTT‑GGC‑TTGTCAGGGTTGTC‑ATAAAAATATGGGCGTCAGCCACGCCGTGTAAACCGATATCATTTGAGAAATTATTATGTTTCg                                                                                               >  6:488402/1‑140 (MQ=255)
                                               aTGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GTC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTAAGCCACGCCCGGTTAAACGATATCTTTTGAGAAATTATTATTTTTc                                                                                                >  2:1168277/1‑138 (MQ=255)
                                                tttCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTAGACTATACGCCACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCa                                                                                             >  8:200090/3‑140 (MQ=255)
                                                  tCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCa                                                                                             >  1:389808/1‑138 (MQ=255)
                                                  tCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGGAAACCGATATCTTTTGAGAAATTATTATGTTTCGCa                                                                                             >  2:514360/1‑138 (MQ=255)
                                                        ttGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAc                                                                                       >  2:9883/1‑138 (MQ=255)
                                                         tGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCAACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTgg                                                                                    >  6:894550/1‑140 (MQ=255)
                                                             gTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGtt                                                                                  >  1:1243685/1‑138 (MQ=255)
                                                              tctcCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAt                                                                               >  8:281007/1‑140 (MQ=255)
                                                                tcCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAttt                                                                             >  5:792782/1‑140 (MQ=255)
                                                                 cgggTTGGGGAAACC‑TTTTTGGGCTGTTCGCTACCATTTT‑GTG‑TGGTCTGGTTTGTC‑AGAACAATTTGGGCCGCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAtt                                                                              <  2:1243556/136‑1 (MQ=255)
                                                                 ccGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTc                                                                            >  3:100613/1‑140 (MQ=255)
                                                                      ttgtttAACT‑TTATGGGGCTCTGCGCGA‑AATTTT‑GGCGTGGTCTGGTTTGTG‑ATAAAAATTTGGGCGACCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGtt                                                                       <  7:587708/135‑1 (MQ=255)
                                                                      ttgtttAACT‑TTACTCGCCTCTTCGCTACTATTTT‑GGC‑GGGACTGGTTTGCA‑ATAACAATAGGGGCGCCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTg                                                                         <  2:385961/133‑1 (MQ=255)
                                                                      ttgggTACAC‑TTATGCGGCGCTGCCCCACAATTTT‑GGG‑TGGTATGTTTTGAC‑ACAAAATTTTGGGCGCCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGtt                                                                       <  3:38693/138‑1 (MQ=255)
                                                                       ttggTAACCATTTTTGGGCGATTCGCCACAATTTG‑GGC‑GGGTGCGGTTTGAA‑ATAAAAATTTGGGCGCCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGtt                                                                       <  3:212652/138‑1 (MQ=255)
                                                                         ggTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATCTTTCGCAAGCGCCTGGTGAATTTCTTGTTCgg                                                                    >  8:384823/1‑140 (MQ=255)
                                                                         gcaaaCC‑TTTTGCGGCTCTACCCCCCAAATTG‑GGC‑TGGCCAGGTGTGAC‑ACACAAATTTGGGCGCCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                                                    <  8:889856/137‑1 (MQ=255)
                                                                           tAACT‑TTTTTGGTCTCTTCCCTACAATTTT‑GGG‑TGGTCTGTTTTTTA‑ACAAAATTTTGGCCGCCTGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                  <  6:262516/140‑1 (MQ=255)
                                                                           aaaCT‑TTATTCGGCTTTCCGCTACCTATTT‑GGC‑TGTTCTGTGTTGCC‑AGAAAAATTTGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTg                                                                  <  4:528690/139‑1 (MQ=255)
                                                                             aCC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                                >  4:122566/1‑140 (MQ=255)
                                                                             aCC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                                >  5:468110/1‑140 (MQ=255)
                                                                              cc‑TTTTGCGGTTCTTCGCGAACAATTT‑GGC‑GGGTATGGTTTGTC‑ATAAAAATTTGGGCGTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg                                                               <  3:98673/140‑1 (MQ=255)
                                                                              cc‑TTATTCGGCTCTTCGCGCCCAATTT‑GGC‑TGGTCAGGTATGTC‑AGAACAATATGGGCGTCAGACACGCCGGGTAAACCGATATCATTTGAGAAATTATTATGTTTCGCAATCGACTGGTTAATTTCTTGTTCGGTGATg                                                               >  6:306091/1‑140 (MQ=255)
                                                                              actttATTCGTATCTTCGCTAAAATTTT‑GTC‑TGTGTAGTTTTGTA‑AAAATAATATGGGCGAAAGCCACGCCGTGTAAACCTATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTTAt                                                                <  4:418859/137‑1 (MQ=255)
                                                                              acgttATTTGGCTCTGCGCTCCAAAGTG‑GGC‑GGGTCCGGTTTGCC‑ATAAAAATTGGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                                <  4:715280/137‑1 (MQ=255)
                                                                               catttttCGGCTTTTCTCCACCATTTT‑GGT‑TGGACTGGTTTTCC‑TCAAAATTTTTGGCGTCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg                                                               <  6:823970/135‑1 (MQ=255)
                                                                                  tATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgtg                                                            >  5:1130520/1‑140 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAACCCGATATCATTTGAGAAATTATAAAGTTTCGCAAGCGACTGGTTAATTTCTAGTTCGGTGATGGAGTa                                                          >  3:1226890/1‑140 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGCCGGGTTAATTTCTTGGTCGGTGATGGTGTa                                                          >  6:270280/1‑140 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgtgtt                                                          >  4:440666/1‑139 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                          >  4:999972/1‑140 (MQ=255)
                                                                                    ttCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGACACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                          >  5:482028/1‑140 (MQ=255)
                                                                                     tCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTAt                                                         >  7:990984/1‑140 (MQ=255)
                                                                                         ctctTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCGGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  3:467984/1‑140 (MQ=255)
                                                                                         ctctTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATTTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  7:745839/1‑140 (MQ=255)
                                                                                         ctctTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGTAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  8:784799/1‑140 (MQ=255)
                                                                                         ctctTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTgtg                                                     >  8:1081732/1‑138 (MQ=255)
                                                                                         ctctTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  4:1071784/1‑140 (MQ=255)
                                                                                         ctctTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  7:465629/1‑140 (MQ=255)
                                                                                         ctctTCGCGAACAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  4:802894/1‑140 (MQ=255)
                                                                                          gttgcgcgACAATTTT‑GGC‑GGGTCTGGTTTGTC‑ATAACAATTTGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGt                                                    <  8:801772/136‑1 (MQ=255)
                                                                                           ctTCGCGACCAATTT‑GGC‑TTGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATTGTGTATTgtg                                                     >  1:548917/1‑136 (MQ=255)
                                                                                           ctTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                     >  1:1051064/1‑138 (MQ=255)
                                                                                           ctTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTAAGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTgct                                                     >  1:588254/1‑136 (MQ=255)
                                                                                            ttCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGCCTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGa                                                  >  7:873939/1‑140 (MQ=255)
                                                                                             tCGCGACCATTTT‑GGA‑TGGTCCGTTTGGTC‑AGAAAAATATGGGGGTCTGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTg                                                   <  2:878989/138‑1 (MQ=255)
                                                                                             acgctACAAATTT‑GGG‑TGGTCTGTTTTGTC‑ATAAAAATTTGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAg                                                 <  6:969638/139‑1 (MQ=255)
                                                                                              cgcgACCAATTT‑GGC‑TGTTCATGTTTGTC‑CGAAAAATATGGGCGTCATCCACGCCGGGTAAACCGAGATCTTTTGAGAAATTATTATGTTTCGCAAGCGTCTGGTTAATTTCTTTTTCGGTGATGGTGTAGTGGGTGAGt                                                >  5:505347/1‑140 (MQ=255)
                                                                                                   ccAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGTAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTTTTCGGTGATGGTGTATTGGGTGAGTTGAtt                                           >  7:79282/1‑140 (MQ=255)
                                                                                                     ttttt‑GGC‑TGGTCAGGTTTGTC‑AGAAAATTATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGAtt                                           <  1:662376/136‑1 (MQ=255)
                                                                                                     ttttt‑GGC‑GGTTCTGGTTTGTA‑CTAACAATATGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTAc                                         <  6:1125409/138‑1 (MQ=255)
                                                                                                     aaTTTGGGC‑TGGTCTGGTTTGTA‑CTAAAAATTTGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTa                                          <  6:792782/140‑1 (MQ=255)
                                                                                                      aTTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCAGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACa                                        >  3:578502/1‑140 (MQ=255)
                                                                                                                 gTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               >  5:289291/1‑140 (MQ=255)
                                                                                                                 gTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               >  8:131481/1‑140 (MQ=255)
                                                                                                                  gtccggttGTCAAGAAAAATTTGGGCGGCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAg                               <  5:510623/134‑1 (MQ=255)
                                                                                                                   cAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCa                             >  3:557458/1‑140 (MQ=255)
                                                                                                                   cAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGAGATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTAAATTTCTTGTTAGGTGATGGTGTATTGGTTGAGGTGATTACAGCCAACGAg                               >  2:150425/1‑138 (MQ=255)
                                                                                                                      gTTTGAC‑AGAAAATTTTGGGCGTCAGCCACGCCGGGTAAACCTATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAg                            <  1:1124738/138‑1 (MQ=255)
                                                                                                                           tC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                     >  6:645369/1‑140 (MQ=255)
                                                                                                                           cc‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                     <  4:372274/139‑1 (MQ=255)
                                                                                                                            taaaaaaaaTATGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                     <  3:913542/133‑1 (MQ=255)
                                                                                                                            aactaaCATTTTGGGGGTCCCCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTGCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCAc                       <  2:42517/134‑1 (MQ=255)
                                                                                                                            aaaaaaCATTATGGGCGACCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGGGAGTTGATTACAGCCAACGAGCAGGCCACTg                     <  7:751847/136‑1 (MQ=255)
                                                                                                                                 aaattatGGGCGGCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGAt                <  6:1075138/136‑1 (MQ=255)
                                                                                                                                     tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt            >  3:829390/1‑140 (MQ=255)
                                                                                                                                     tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAGGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAAt            >  6:684670/1‑140 (MQ=255)
                                                                                                                                            cttaGCCACGCCGGGTAAACCGATCTGTTTTGAGAAATTATTATTTTTCGCAAGCGACTGGATACTTTCTTGTTAGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGc       <  2:980174/135‑1 (MQ=255)
                                                                                                                                             tCAGCCACTCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAATTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  3:955372/1‑140 (MQ=255)
                                                                                                                                             tCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGCTTACAGCCAACGAGCAGTCCACTGACGATCAATGCAGCGGc    >  5:1061649/1‑140 (MQ=255)
                                                                                                                                             tCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  7:30712/1‑140 (MQ=255)
                                                                                                                                             tCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    <  6:289291/140‑1 (MQ=255)
                                                                                                                                             tCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCAACTGACGATCAATGCAGCGGc    >  4:924868/1‑140 (MQ=255)
                                                                                                                                             tCAGCCACGCCGGGGAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACTAGCAGGCCACTGACGACCAATGCAGCGGc    >  7:332251/1‑140 (MQ=255)
                                                                                                                                               aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACCGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaa  >  3:535292/1‑140 (MQ=255)
                                                                                                                                               aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc    >  2:62920/1‑138 (MQ=255)
                                                                                                                                               aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaa  >  4:133428/1‑140 (MQ=255)
                                                                                                                                               aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaa  >  6:1196372/1‑140 (MQ=255)
                                                                                                                                               aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaa  >  7:56112/1‑140 (MQ=255)
                                                                                                                                               aGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGAGACTGGTTAATTTCTTGTTCGGTGATGGTGGATTGGGTGCGTGGATGACAGCCACCGCGCAGGCCACTGCCGATCAATGCAGCGGGaa  >  4:1140659/1‑140 (MQ=255)
                                                                                                                                               |                                                                                                                                           
TCAGCTTCAGTTTCATGGTCGCTTTCTGGCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACC‑TTATTCGGCTCTTCGCGACCAATTT‑GGC‑TGGTCAGGTTTGTC‑AGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAA  >  NZ_CP022686/4712624‑4712902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A