Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F13 I0 R1 206 15500.2 9478722 98.3% 9317583 135.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,951,538 C→A 21.9% A44A (GCC→GCA)  topA → type I DNA topoisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,951,5380CA21.9% 278.0 / 24.9 171A44A (GCC→GCA) topAtype I DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base C (80/49);  new base A (2/40);  total (82/89)
Fisher's exact test for biased strand distribution p-value = 8.97e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

GGTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAG  >  NZ_CP022686/4951400‑4951676
                                                                                                                                          |                                                                                                                                            
 gTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGACGACCAGTGGCTCAACTGCC‑aa                                                                                                                                           >  4:1158791/1‑140 (MQ=255)
         gggTAACGCTGTTGTCATCTTTGAGGCCCCGGCAAAAGCCAAAACTATCAACAAGTATCTGGGTTGTGACTACGGGGTTAAATCCAGCGTCGGCAACATCCGCTATTTGCCCACCAGTGGCTCAACTGCA‑aaaacgtg                                                                                                                                     <  6:213685/138‑5 (MQ=255)
         gggTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGCCCAGTGGCTCAACTGCC‑AAAAA‑GAGTg                                                                                                                                  >  8:1035226/1‑140 (MQ=255)
          ggTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGc                                                                                                                                 >  4:1056806/1‑140 (MQ=255)
           taaaaGCTCTTGTCATCGTTGAGTCCCCGGAAAAAGCAAAAACGATAAACAATTATCTGGGCAGTGACTACGTGGTTAAATCCAGCGTCGGTAACATCCGCTATTTGCCGACCTGTGGCTCAACTGCA‑AAAAC‑GAGTg                                                                                                                                  <  6:708315/136‑1 (MQ=255)
           gTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGCAATCCAGCGTCGGTCACATCCGAGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGcc                                                                                                                                >  7:397383/1‑140 (MQ=255)
           gTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGcc                                                                                                                                >  4:1079082/1‑140 (MQ=255)
           gTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGcc                                                                                                                                >  3:119595/1‑140 (MQ=255)
           gTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCAAAAACGATCAACAAGTCTCTGGGTAGTGACTACGTGGGGAAATCCAGCGTCGGTCCCATCCGCGATTTGCCGACCAATGGCGCACCGGCC‑AAAAA‑GGGTGcc                                                                                                                                >  8:258527/1‑140 (MQ=255)
             aaaGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGcc                                                                                                                                >  5:458578/1‑138 (MQ=255)
             aaaGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGcc                                                                                                                                >  1:22655/1‑138 (MQ=255)
             aaaGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGcc                                                                                                                                >  6:1153515/1‑138 (MQ=255)
             aaaGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGcc                                                                                                                                >  2:786664/1‑138 (MQ=255)
             aaaGCTCTTCTCATCGTTGAGTCCCCGGCACAAGCCAAAACGATCCACAAGTATCTGGGTAGTGACTACGTGGTGAAATCAATCGTCGGTCACATACGCGATTTGACTACCATTTGCTAATCTACC‑AAATT‑GAGTGcc                                                                                                                                >  5:749389/1‑138 (MQ=255)
              aaGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAAGCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGAc                                                                                                                             >  7:789523/1‑140 (MQ=255)
                gCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGAc                                                                                                                             >  2:813943/1‑138 (MQ=255)
                    ttGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGACATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGCCTCTAcc                                                                                                                       >  8:1327/1‑140 (MQ=255)
                    ttGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTAcc                                                                                                                       >  8:658950/1‑140 (MQ=255)
                    ttGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATACGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTAcc                                                                                                                       >  4:564348/1‑140 (MQ=255)
                      gTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTAcc                                                                                                                       >  1:101668/1‑138 (MQ=255)
                      gTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTAcc                                                                                                                       >  1:72608/1‑138 (MQ=255)
                      gTCATCGGTGCGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTATTGACTACGTGGTGAATTCCAGCGTCGGTCACATCCGCGCTTTGCCGACAAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGCCTCAAcc                                                                                                                       >  6:507921/1‑138 (MQ=255)
                             ttGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCTCCTCTACCTccacca                                                                                                                >  1:563713/1‑138 (MQ=255)
                             ttGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTccacca                                                                                                                >  2:1203981/1‑138 (MQ=255)
                             ttGAGTCACCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTacacca                                                                                                                >  5:562863/1‑138 (MQ=255)
                              tGAGTCCCCGGCAAAAGCCAAAACGATCAACATGTATCTGGGTCTTCACTACGTGTGCAAAACCAGCGGGGGTCACATCCGCTTTTGGCCGACCTGGGGCTCAACTCCA‑AAAAC‑GAGTGCCGACTCTACCTCCACCAAGa                                                                                                             <  4:983451/140‑1 (MQ=255)
                                    cccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTa                                                                                                       >  3:924702/1‑140 (MQ=255)
                                      cGGCAAAAGCCAAAACGAGCAACACTAATGTGGGTAGTGACTCGGTGTTTAAATCCCGCGTCGGTCACTTCCGCGAGTTGCCCACATGGGGCCAAACCCCC‑AAAAA‑CAGGGCCGACTCTACCTCCACCAAGACGGCTaaa                                                                                                     <  4:141273/140‑1 (MQ=255)
                                         cAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGGTCAACTGCA‑AAAAA‑GAGAGCCGACTCTCCCTCCACCAAGACGGCTaaaa                                                                                                    >  6:931903/1‑138 (MQ=255)
                                            aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCt                                                                                               >  7:478459/1‑140 (MQ=255)
                                              gCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCt                                                                                               >  5:783922/1‑138 (MQ=255)
                                              gCCAAAACGATCAACAAGTATCTGGCTAGTGACTACGTGGAGAAATCCAGCGTCGCTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTCCCTCCACCAAGACGGCTAAAAAGCCt                                                                                               >  6:339076/1‑138 (MQ=255)
                                               ccAACACTATCAACAAGTAGCGGGGTGGTGACTCGTTGGTGAAATCCAGCGGCGGTCACAGCCGGTATTTGCCGACCTGTGGCTCAACTGCC‑AAAAA‑GTGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTa                                                                                              <  5:1080952/138‑1 (MQ=255)
                                               ccAAACCGATAAACTAGTTTCTGGTTTTTTAATAGGTGGTTAAATCCTGCGGGGGTCAAATCCGCTATTTGCCGACCAGTGGCTAAACTGCA‑AAAAA‑GTGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTaaa                                                                                            <  4:581283/140‑1 (MQ=255)
                                                aaaaaCCTTCAAAATGTATGTGGGTAGTGAATACGTGTTGAAATCCAGCGGCGGCCCAATCCGCGATTGGCCTACCAGGGGCTAAACTGCC‑AAAAA‑GAGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTaa                                                                                             <  6:268601/137‑1 (MQ=255)
                                                            caaGTTTCTGGGTAGTGAGTAGGTGGTTAAATCCAGCGTCGTCCACATCCGCGTTTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACg                                                                                 <  6:1057337/138‑1 (MQ=255)
                                                               ttagcTGGGTAGTGACACCTTGGTGAAATCCCGCGGCGGTCACTTCCGCGATTGCCCGACCAGTGGGTCACCTGCA‑TAAAT‑TTGTGCCGACTCTACCTCATCCAATACGGCTAAAAAGCCTAAAAAGGATGAACGTggag                                                                            <  4:804638/136‑3 (MQ=255)
                                                               ttATCTGGGTCGTCCCTCCGTGGTCAAACCCAGCGGCGGTCACAGCCGCGATTTGCCGACCTGTGGCTCAACTGCA‑AAAAA‑GCGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTgg                                                                              <  6:179111/137‑1 (MQ=255)
                                                               gtagcggggTAGTGACTAGGTGGTGAAACCCCGCGGCGGTCACAGCCGCGATTTGCCGACCAGTGGCTCACCTCCC‑AAAAC‑GCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                            <  8:627044/134‑1 (MQ=255)
                                                               gTATCTGGGTCGTGAATACGTGGTGAAATCCCGCGTCGGTCACATCCGCTATTTGCCGACCAGTGGCTAAACTGCA‑AAAAA‑GAGCGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTgg                                                                              <  2:72608/138‑1 (MQ=255)
                                                               gTATCTGGGTAGTGACTACGTGGTTAAATCCAGCGGCGGTCCCAGCCGCGATTTGCCGACCAGTGTTGCAACTGCA‑AAAAA‑GATGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                            <  8:506311/140‑1 (MQ=255)
                                                               gTATCGGGGGTGTGACTACGTGGTAAAATCCCGCGGCGGTCACATCCGCTATTTGCCCACCTGTGGCTCACCTGCA‑AAAAA‑GAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTgg                                                                              <  1:1143950/138‑1 (MQ=255)
                                                               gTAGCTGGGTTTTTACTATGTGGTTAAATCCAGCTCCGGAAACATCGGCTATTTCCCGACATTTGGCTAAATTTCA‑AAAAA‑GAGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                            <  4:853585/140‑1 (MQ=255)
                                                                 tgcTGGTTAGTGAATACGTGGTTAAATCCCGCGTCGGTCACATCCGCGATTTGCCGACCTGTGGCACAACTGCA‑AAAAA‑CCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                            <  2:941749/136‑1 (MQ=255)
                                                                 aTCTGGGTTGGTAATACGTGGTTAAATCCAGCGTCGGACACATCCGCGATTTGCCCACCTGGGGCTAAACTGCC‑AAAAC‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGct                                                                          <  3:625449/140‑1 (MQ=255)
                                                                    tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGAGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGctc                                                                         >  5:1118978/1‑138 (MQ=255)
                                                                      ggTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCGAAAAAGCCTAAAAAGTAGGAACGTGGCGCTCTCGt                                                                     >  8:826097/1‑140 (MQ=255)
                                                                       gTAGTCACTACGTGGTGAAAACCAGCGCGGTTAACACCCGCTATTTGCCCACCAGGGGCTCAACTGCC‑AAAAA‑GCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCg                                                                      <  2:791281/138‑1 (MQ=255)
                                                                         tgTGACTACGTGGTCAATTCCAGCGGCGGGAACATCCGGGATTGGCCTACCTGTGGCTCAACTGCA‑AAAAA‑TCGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                  <  7:866728/139‑1 (MQ=255)
                                                                         cgTGACTCCGTGGTCAAAACCAGCGGCGGACACATCCGCGATTGCCCGAACTGTGCCTAAACTGCA‑AAAAG‑GCGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                  <  7:597004/139‑1 (MQ=255)
                                                                         aGTGACTCCGTGGTTAAATCCAGGGGCGGACACATCCGCTATTTGCCCACCAGGGGCTAAACTGCC‑AAAAC‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTc                                                                    <  1:672581/138‑1 (MQ=255)
                                                                         aGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACAAGTGGATCAACTGCC‑AAAAA‑GAGTGCCGACTCTCCCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTc                                                                    >  1:336184/1‑138 (MQ=255)
                                                                          gTGACTACGTCTTGAAATCCAGCGTCGGTCACAACCTCGAGTTCCCGACCAGTGGCACACCTGCC‑AAAAA‑GAGGGCCGACTCTAACTCCACGAAGCCCGCATAAAAGCCTAAAAAGTATGAACGATGCtctctctactcc                                                                 >  8:279672/1‑134 (MQ=255)
                                                                             aCTACGTGGTGAAATCCAGCGTCGGTCACCTCCGCGAGTTGCCGCCCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTAACTCCACCAAGACGGCTAAAAAGCCAAAAAAGGATGAACGTGGCGCTCTCGTCAAcc                                                                >  6:109536/1‑138 (MQ=255)
                                                                             aCTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGCCCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAcc                                                                >  2:684095/1‑138 (MQ=255)
                                                                               tccGTGGTTAAATCCAGGGTCGGTCACATCCGCTATTTGCCGACATGTGGCACAACTGCA‑AAAAA‑GAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGt                                                              <  1:133899/136‑1 (MQ=255)
                                                                               tACGTGGTAAAACCCCGCGGCGGTCACAGCCGCTATTGGCCGACCTGGGGCACAACAGCC‑AAAAC‑GAGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGt                                                              <  5:699565/138‑1 (MQ=255)
                                                                               atcGTGGTGAACCCCCGCGTCGGCCACATCCGCGATTTGCCGACCAGTGGCCCAACAGCA‑AAAAA‑GAGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGt                                                              <  1:1004971/136‑1 (MQ=255)
                                                                               aaggtggtgAAATCCCGCGTCGGTCACAGCGGCGATTTGCCGACCAGGGGCACACCTGCA‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAt                                                            <  3:177996/137‑1 (MQ=255)
                                                                                aCGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAACCGCCTAAAAAGGATGACCGTGGCGCTCTCGTCAACCGGATg                                                           >  4:799575/1‑140 (MQ=255)
                                                                                  gtggtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATg                                                           >  5:305439/1‑138 (MQ=255)
                                                                                  gtggtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATg                                                           >  2:861872/1‑138 (MQ=255)
                                                                                  gtggtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATg                                                           >  2:1039754/1‑138 (MQ=255)
                                                                                   tggtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCACCCGTAttg                                                          >  1:1144925/1‑136 (MQ=255)
                                                                                      tcaaaTCCCGCGGCGGTCACATCCGCGATTTGCCCACCTGGGGCTCAACTGCA‑AAAAC‑GCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATggggg                                                       <  2:425071/136‑1 (MQ=255)
                                                                                           tCCAGCGTCGGTCATATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGcc                                                                                                                                >  3:1095974/1‑60 (MQ=255)
                                                                                           tCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGAccc                                                >  7:976070/1‑140 (MQ=255)
                                                                                            cccgcggcgGTCACATCCGCGTTTTGCCGACCAGGGGCTCAACTGCA‑AAAAA‑GCGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCg                                               <  3:1167304/137‑1 (MQ=255)
                                                                                             cAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGCCTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCACCCGTATGGGGGTTGcccc                                                >  1:966234/1‑138 (MQ=255)
                                                                                               gtggcGGTAACAACCGCTATTTGCCCACCAGGGGC‑GAACAGCCAAAAAA‑GCGCGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                            <  8:619164/136‑1 (MQ=255)
                                                                                               gcggcgGTCCCAGCCGCGATTTGCAGACCAGGGCCTCAACTGCC‑AAAAC‑GCGCGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGt                                              <  6:582695/138‑1 (MQ=255)
                                                                                               gcggcgGTCACAGCCGCGATGTGCCGACCCTGGGCCCACCTGCC‑AAAAC‑CAGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGGGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                            <  3:674139/140‑1 (MQ=255)
                                                                                               gcggcgGGCACAGCCGCGCTTTGCCGACCTGGGGAGCAACTGCC‑AAACC‑CCGTGCCGACTCTACCTCCCCCAAGACGTCTAAAAAGCCTAAAAAGGATGACCGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGt                                              <  6:576939/138‑1 (MQ=255)
                                                                                               gcggcgGCCACATCCGCGATTTGCCGCCCAGGGGCGCAACTCCA‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                            <  3:14381/140‑1 (MQ=255)
                                                                                               gcggcgGCCACACCCGCTATTTGCCGACCTGTGCCCCAACACCC‑AAAAA‑GAGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGt                                              <  2:854075/138‑1 (MQ=255)
                                                                                               gcgcCGGTCAACTCGGCGATTGCCCCCCCAGTGCCCAAACTGCA‑AAAAT‑CAGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGGGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGt                                              <  5:237715/138‑1 (MQ=255)
                                                                                               gCGTCGGTCAAATCCGCTATTTGCCTACCAGGGGCTCAAATCCC‑AAAAA‑GCGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGt                                              <  1:789315/138‑1 (MQ=255)
                                                                                               ccGTCGGTCACACCCGCGATTTCCCGACCTGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGt                                              <  2:52329/137‑1 (MQ=255)
                                                                                                 ggcGGTCACATCCGCGATTTGCCTACCTGGGGCACAACTGCA‑AAAAA‑GCGGCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGca                                          <  8:1103061/138‑1 (MQ=255)
                                                                                                 ggcGGGCACATCCGCGTTTGGCCGACCAGTGGCTCAACTCCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                            <  6:305439/136‑1 (MQ=255)
                                                                                                  tCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGc                                           >  5:292771/1‑138 (MQ=255)
                                                                                                    ggTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGAGGCGCACTCGTCAACCGTATGGGGGTTGACACGTGGcac                                         >  5:364500/1‑138 (MQ=255)
                                                                                                       cacaTCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGCCCCGTGGCACAAt                                      >  1:530448/1‑138 (MQ=255)
                                                                                                             cgcgATTTGCCCCCCAGTGGCGCACATGCC‑AAAAA‑GAGTCCCGACTCTACCTCCACCAAGACGGCTAAACAGCCTAAAAAGGATGATCGTGGCGCTCTCGTCAACCGTATGGGGGGTGACCCGTGGCACAATTGGGAGgc                              <  4:457560/140‑1 (MQ=255)
                                                                                                              gtgttttGCCGCCATGGGGCGCAACTGCC‑AAAAG‑GAGTGCCGACTCTACGTCCACGAAGACGGCTAATAAGGCTAAAAAGGTTGATCGTGGCGCTCTCGTCAAACGTATGGGGGTTGACCCGTGGCACAATTGGGAgg                               <  1:934561/134‑1 (MQ=255)
                                                                                                              gctttttGCCGACCAGTGGCTCAACGCCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcg                             <  7:760479/136‑1 (MQ=255)
                                                                                                              gctaTTTGCCGACCAGTGGATCAACTGCA‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcg                             <  3:379204/137‑1 (MQ=255)
                                                                                                               gtttttGCCGACCAGTGGCTCAATTGCA‑AAAAT‑GCGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcgc                            <  3:943620/137‑1 (MQ=255)
                                                                                                               cgATTTGCCTCCCAGGGGCCCAACTCCA‑AAAAA‑GAGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgc                              <  1:459162/138‑1 (MQ=255)
                                                                                                                taTTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCa                           <  3:1056806/139‑1 (MQ=255)
                                                                                                                taTTTGCCGACATGTGGCTAAAATGAA‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCa                           <  7:291663/139‑1 (MQ=255)
                                                                                                                     gCCGACACGTGCCTCAACTCAC‑AAAAA‑GTGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAATGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATg                      <  8:474399/140‑1 (MQ=255)
                                                                                                                          cccggggCCCATCTGAC‑AAAAG‑GTGTCCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAg                   <  2:962683/133‑1 (MQ=255)
                                                                                                                              tGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGc             >  7:543395/1‑140 (MQ=255)
                                                                                                                              gggCTCAATTGCA‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGc             <  7:500983/139‑1 (MQ=255)
                                                                                                                              gggCTCAAC‑GCC‑AAAAATGAGGGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGtt               <  2:257894/137‑1 (MQ=255)
                                                                                                                               ggCTAAAATGCC‑AAAAC‑TAGTCCCGACTCTACCTCCACCACGACGGCTAAACAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTg              <  6:984309/138‑1 (MQ=255)
                                                                                                                                 cTCAAGTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGcc            <  5:411350/138‑1 (MQ=255)
                                                                                                                                   caccTGCA‑AAAAA‑GTTGGACGACTCTCCCTCCCCCAAGACGGCTAAAAAGCCTAAAAAGGATGACCGTGGCTCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGt        <  3:667250/137‑1 (MQ=255)
                                                                                                                                          c‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGTCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATAGGGAGGCTCACTATGAAGTGTTGACTGGTAAaga   >  5:63452/1‑138 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GGTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAACTGCC‑AAAAA‑GAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAG  >  NZ_CP022686/4951400‑4951676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A