Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I206 R1
|
206 |
23.7 |
1314362 |
96.8% |
1272302 |
86.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,064,259 |
T→G |
V93G (GTG→GGG) |
BW25113_RS10440 → |
GTPase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,064,259 | 0 | T | G | 96.0%
| 75.2
/ ‑3.3
| 25 | V93G (GTG→GGG) | BW25113_RS10440 | GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (14/10); total (15/10) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
GTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCAC > NZ_CP009273/2064176‑2064345
|
gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt > 1:502933/1‑90 (MQ=255)
gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt > 2:580736/1‑90 (MQ=255)
gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt > 2:574183/1‑90 (MQ=255)
gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt > 2:314762/1‑90 (MQ=255)
tgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGttt > 1:571180/1‑90 (MQ=255)
ggCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTtg > 2:413555/1‑90 (MQ=255)
aaGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGc < 2:67092/90‑1 (MQ=255)
gATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc > 1:373374/1‑90 (MQ=255)
gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGc > 2:654106/1‑61 (MQ=255)
gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGc < 1:654106/61‑1 (MQ=255)
gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa > 2:140837/1‑90 (MQ=255)
gTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATg < 1:110977/90‑1 (MQ=255)
ggCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATgg > 2:260537/1‑90 (MQ=255)
cGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc < 2:165080/90‑1 (MQ=255)
gTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtc < 2:18253/90‑1 (MQ=255)
tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtct > 2:425466/1‑90 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc < 2:274843/74‑1 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc > 1:274843/1‑74 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtctc > 2:115807/1‑90 (MQ=255)
aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac < 2:547123/90‑1 (MQ=255)
aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac < 1:590820/90‑1 (MQ=255)
aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac < 1:413555/90‑1 (MQ=255)
cagcagGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAACGGGATATGGCCGGTATTCAGCCTTCTCCTgcacag > 1:580122/1‑90 (MQ=255)
gcagGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcacaggc > 2:293377/1‑90 (MQ=255)
agGGGCTGTTTGTGGTGACGCAGGCCGACAAACCGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcacaggcac < 2:64112/90‑1 (MQ=255)
|
GTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCAC > NZ_CP009273/2064176‑2064345
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCTGGTACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCT‑TCTCCTGCACAGGCACAGAATAT > NZ_CP009273/2064166‑2064352
|
ACCTGGTACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTT > SRR3722088.508978/1‑100 (MQ=60)
CCTGGTACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTT > SRR3722088.578183/1‑100 (MQ=60)
GATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCC > SRR3722088.377346/1‑100 (MQ=60)
agatgtgtataagagacagGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAA < SRR3722088.662211/81‑1 (MQ=60)
GTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCC < SRR3722088.112129/100‑1 (MQ=60)
ATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTGTCT > SRR3722088.277493/1‑100 (MQ=60)
GTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAACGGGATATGGCCGGTATTCAGCCT‑TCTCCTGCACAG > SRR3722088.587232/1‑100 (MQ=60)
CATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCT‑TCTCCTGCACAGGCACAGA > SRR3722088.311612/1‑100 (MQ=60)
ATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCT‑TCTCCTGCACAGGCACAGAA < SRR3722088.418124/100‑1 (MQ=60)
ATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCT‑TCTCCTGCACAGGCACAGAA < SRR3722088.598075/100‑1 (MQ=60)
AGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCT‑TCTCCTGCACAGGCACAGAATAT > SRR3722088.647583/1‑100 (MQ=60)
|
ACCTGGTACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCT‑TCTCCTGCACAGGCACAGAATAT > NZ_CP009273/2064166‑2064352
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
