Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I227 R1
|
223 |
21.3 |
1167702 |
97.0% |
1132670 |
86.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,064,259 |
T→G |
V93G (GTG→GGG) |
BW25113_RS10440 → |
GTPase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,064,259 | 0 | T | G | 95.8%
| 69.8
/ ‑3.3
| 24 | V93G (GTG→GGG) | BW25113_RS10440 | GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (11/12); total (11/13) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01 |
TACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCAC > NZ_CP009273/2064172‑2064345
|
tACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGc < 1:223004/90‑1 (MQ=255)
gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt > 1:244394/1‑90 (MQ=255)
gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt > 1:208945/1‑90 (MQ=255)
gATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc > 2:137920/1‑90 (MQ=255)
aTGACCGTGCCCTGTATGTGGATGAGAATTTCTGGCGACAAATCCTTCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcca > 1:482150/1‑89 (MQ=255)
cccTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACgg < 2:459247/90‑1 (MQ=255)
tgtctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGc < 2:208945/90‑1 (MQ=255)
ggATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCa < 2:244394/90‑1 (MQ=255)
gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa > 2:460913/1‑90 (MQ=255)
gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa > 1:47268/1‑90 (MQ=255)
gTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATg > 1:262355/1‑90 (MQ=255)
tCTGGCGACACATCCTGCAGTGCGGACCTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGata < 2:137362/90‑1 (MQ=255)
cGACACATCCTGCANTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc < 2:361299/90‑1 (MQ=255)
cGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGcc < 2:403737/90‑1 (MQ=255)
tCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTc < 1:365872/90‑1 (MQ=255)
cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt < 2:61575/90‑1 (MQ=255)
gTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGAc > 2:258023/1‑43 (MQ=255)
gTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGAc < 1:258023/43‑1 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc < 1:538691/38‑1 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc > 2:538691/1‑38 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtctc > 1:543675/1‑90 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtctc > 1:434236/1‑90 (MQ=255)
tCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcaca < 2:371802/90‑1 (MQ=255)
agGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcacaggcac < 2:169290/90‑1 (MQ=255)
|
TACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCAC > NZ_CP009273/2064172‑2064345
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCTGGTACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATA > NZ_CP009273/2064166‑2064351
|
ACCTGGTACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTT > SRR3722111.211507/1‑100 (MQ=60)
ACCTGGTACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTT > SRR3722111.247289/1‑100 (MQ=60)
tcgtcggcagcgtcagatgtgtataagagacaGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTT < SRR3722111.13766/68‑1 (MQ=60)
TACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGT < SRR3722111.225705/100‑1 (MQ=60)
ATTAAAGCCGATGACCGTGCCCTGTATGTGGATGAGAATTTCTGGCGACAAATCCTTCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCA > SRR3722111.489542/1‑100 (MQ=60)
cattgacattcgtcggcagcgtcagatgtgtataagagacagATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGG < SRR3722111.547155/58‑1 (MQ=60)
acattcgtcggcagcgtcagatgtgtataagagacagACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGC < SRR3722111.261060/63‑1 (MQ=60)
GTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAA > SRR3722111.47846/1‑100 (MQ=60)
CTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATG > SRR3722111.265455/1‑100 (MQ=60)
ATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTC > SRR3722111.440727/1‑100 (MQ=60)
ATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTC > SRR3722111.552226/1‑100 (MQ=60)
TCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCC < SRR3722111.371014/100‑1 (MQ=60)
CAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATA > SRR3722111.81929/1‑100 (MQ=60)
|
ACCTGGTACTGTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGAATA > NZ_CP009273/2064166‑2064351
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
