Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I205 R1
|
220 |
17.5 |
961422 |
97.1% |
933540 |
86.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,064,259 |
T→G |
V93G (GTG→GGG) |
BW25113_RS10440 → |
GTPase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,064,259 | 0 | T | G | 100.0%
| 84.3
/ NA
| 25 | V93G (GTG→GGG) | BW25113_RS10440 | GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (15/10); total (15/10) |
GTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCAC > NZ_CP009273/2064176‑2064339
|
gtgGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGtt > 2:244789/1‑90 (MQ=255)
ggCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTtg > 2:140787/1‑90 (MQ=255)
gATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGcc > 1:22109/1‑90 (MQ=255)
gtctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGcc > 2:96958/1‑90 (MQ=255)
gtctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGcc > 2:62409/1‑90 (MQ=255)
tctgtGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGccc < 2:22109/90‑1 (MQ=255)
ggATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCa > 2:426662/1‑90 (MQ=255)
gATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCAt < 1:244789/90‑1 (MQ=255)
gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa > 1:386433/1‑90 (MQ=255)
gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa > 2:353103/1‑90 (MQ=255)
gAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGGGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGaa > 2:9288/1‑90 (MQ=255)
aGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAAt < 1:65539/90‑1 (MQ=255)
gTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAAc < 1:409491/72‑1 (MQ=255)
gTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAAc > 2:409491/1‑72 (MQ=255)
ggCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATgg > 2:166654/1‑90 (MQ=255)
ggCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATgg > 2:480342/1‑90 (MQ=255)
acacATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCgg < 1:96958/90‑1 (MQ=255)
tCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTc < 1:9579/90‑1 (MQ=255)
cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt < 1:217922/90‑1 (MQ=255)
cAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt < 1:166654/90‑1 (MQ=255)
tGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtct > 1:372810/1‑90 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt > 1:98605/1‑86 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCt < 2:98605/86‑1 (MQ=255)
gCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTtctc > 2:427324/1‑90 (MQ=255)
aTCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTgcac < 2:205039/90‑1 (MQ=255)
|
GTGGCTGATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCAC > NZ_CP009273/2064176‑2064339
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGA > NZ_CP009273/2064182‑2064348
|
GATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCC > SRR3722087.22276/1‑100 (MQ=60)
agagacagCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGC < SRR3722087.414254/92‑1 (MQ=60)
GTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAA > SRR3722087.390890/1‑100 (MQ=60)
cgccttagtctcgtgggctcggagatgtgtataagagacagAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGAT < SRR3722087.336912/59‑1 (MQ=60)
GATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATA < SRR3722087.247127/100‑1 (MQ=60)
AGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGC < SRR3722087.66117/100‑1 (MQ=60)
ACACATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCT < SRR3722087.97893/100‑1 (MQ=60)
CATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCT > SRR3722087.377083/1‑100 (MQ=60)
ATCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTC > SRR3722087.99549/1‑100 (MQ=60)
TCCTGCAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCC < SRR3722087.9661/100‑1 (MQ=60)
CAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCAC < SRR3722087.168172/100‑1 (MQ=60)
CAGTGCGGACATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCAC < SRR3722087.219847/100‑1 (MQ=60)
CATCAGCAGGGGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGA > SRR3722087.165458/1‑100 (MQ=60)
|
GATTAAAGCCGATGACCGTGCCCTGTCTGTGGATGAGTATTTCTGGCGACACATCCTGCAGTGCGGACATCAGCAGGTGCTGTTTGTGGTGACGCAGGCCGACAAAACGGAGCCCTGCCATGAATGGGATATGGCCGGTATTCAGCCTTCTCCTGCACAGGCACAGA > NZ_CP009273/2064182‑2064348
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
