Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I210 R1
|
226 |
19.6 |
1130256 |
95.4% |
1078264 |
84.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
4,034,572 |
T→G |
Q74H (CAA→CAC) |
mobB ← |
molybdopterin‑guanine dinucleotide biosynthesis protein MobB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 4,034,572 | 0 | T | G | 100.0%
| 96.4
/ NA
| 30 | Q74H (CAA→CAC) | mobB | molybdopterin‑guanine dinucleotide biosynthesis protein MobB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (13/17); total (13/17) |
TCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATG > NZ_CP009273/4034486‑4034658
|
tCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTgc < 2:217989/90‑1 (MQ=255)
gCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTgctggc < 1:193879/90‑1 (MQ=255)
gCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTgctggc > 1:413654/1‑90 (MQ=255)
tttGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTAATCAAGGCCCATCGGTgctggctg < 1:18424/90‑1 (MQ=255)
tATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGa < 1:57668/90‑1 (MQ=255)
tATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGa > 2:69834/1‑90 (MQ=255)
aTCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGAt > 2:504477/1‑90 (MQ=255)
aTCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGAt > 1:509233/1‑90 (MQ=255)
ttCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGttt > 2:389534/1‑90 (MQ=255)
aaaCTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGc < 1:504477/90‑1 (MQ=255)
aaCTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGcc > 1:441188/1‑90 (MQ=255)
aGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTgcgc < 2:8399/90‑1 (MQ=255)
gATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCa < 2:312363/90‑1 (MQ=255)
cAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTc < 1:278232/90‑1 (MQ=255)
aGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTgcgc > 2:128678/1‑84 (MQ=255)
aGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTgcgc < 1:128678/84‑1 (MQ=255)
aGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCa > 2:401494/1‑90 (MQ=255)
cttcttCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGc < 2:86516/90‑1 (MQ=255)
tcttcGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGa > 2:243036/1‑52 (MQ=255)
tcttcGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGa < 1:243036/52‑1 (MQ=255)
tcttcGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTa > 1:503022/1‑90 (MQ=255)
tcGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCt > 2:18383/1‑90 (MQ=255)
cGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCtt < 1:222141/90‑1 (MQ=255)
gTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCa < 2:518544/90‑1 (MQ=255)
caAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATc < 2:509233/90‑1 (MQ=255)
aGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCa < 2:147814/90‑1 (MQ=255)
cATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGc < 2:143368/58‑1 (MQ=255)
cATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGc > 1:143368/1‑58 (MQ=255)
cATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCa > 2:391157/1‑90 (MQ=255)
tCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATg < 1:25377/90‑1 (MQ=255)
|
TCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATG > NZ_CP009273/4034486‑4034658
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTA > NZ_CP009273/4034473‑4034671
|
GACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGG < SRR3722092.161421/100‑1 (MQ=60)
ATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGC > SRR3722092.421633/1‑100 (MQ=60)
TCAAATCCAGCTTTGAGGTATCCATTCGGCTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCT < SRR3722092.31241/100‑1 (MQ=60)
GCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGAT < SRR3722092.197100/100‑1 (MQ=60)
GCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGAT > SRR3722092.519434/1‑100 (MQ=60)
TTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTAATCAAGGCCCATCGGTGCTGGCTGGCAACGATGG < SRR3722092.18742/100‑1 (MQ=60)
ggcagcgtcagatgtgtataagagacAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGC < SRR3722092.246973/74‑1 (MQ=60)
TATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGC < SRR3722092.58602/100‑1 (MQ=60)
ACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCC > SRR3722092.449849/1‑100 (MQ=60)
AAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGC < SRR3722092.514562/100‑1 (MQ=60)
AGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGC < SRR3722092.130849/100‑1 (MQ=60)
ATACAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTA > SRR3722092.513080/1‑100 (MQ=60)
CAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCT < SRR3722092.282985/100‑1 (MQ=60)
CGTCTGGTGTTTCCGTCATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTT < SRR3722092.225772/100‑1 (MQ=60)
CATCAAGGCCCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCctgtctcttatacacatctgac > SRR3722092.145775/1‑78 (MQ=60)
CCATCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCctgtctcttatacacatctgacgctgccgac > SRR3722092.198352/1‑69 (MQ=60)
TCGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGC < SRR3722092.25815/100‑1 (MQ=60)
CGGTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCT < SRR3722092.84889/100‑1 (MQ=60)
GTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTA > SRR3722092.262462/1‑100 (MQ=60)
|
GACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTA > NZ_CP009273/4034473‑4034671
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
