Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,592 G→T 89.2% D814Y (GAC→TAC)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,5920GT89.2% 604.3 / 73.8 229D814Y (GAC→TAC) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base G (9/16);  new base T (80/123);  total (90/139)
Fisher's exact test for biased strand distribution p-value = 8.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 231 total aligned reads displayed.

CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  CP009273/557445‑557741
                                                                                                                                                   |                                                                                                                                                     
 cGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACg                                                                                                                                                    <  2:3196309/150‑1 (MQ=255)
  gACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACgg                                                                                                                                                   <  2:2906311/150‑1 (MQ=255)
   aCGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACggg                                                                                                                                                  <  2:2240298/150‑1 (MQ=255)
   aCGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACggg                                                                                                                                                  <  2:3361229/150‑1 (MQ=255)
    cGCAAGGCGCGTTGGTTCGTGTCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGc                                                                                                                                                 <  1:393791/150‑1 (MQ=255)
    cGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGc                                                                                                                                                 <  2:80813/150‑1 (MQ=255)
    cGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGc                                                                                                                                                 <  1:4108278/150‑1 (MQ=255)
     gCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCa                                                                                                                                                >  2:1325803/1‑150 (MQ=255)
      cAAGGCGCGTTGGTTCGTGCCAATTTTGCTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                               <  1:2582504/150‑1 (MQ=255)
      cAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                               <  2:2480205/150‑1 (MQ=255)
      cAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                               <  1:2767670/150‑1 (MQ=255)
      cAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                               <  1:2210523/150‑1 (MQ=255)
       aaGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAg                                                                                                                                              >  2:3692370/1‑150 (MQ=255)
          gcgcGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGttt                                                                                                                                           <  2:2541360/150‑1 (MQ=255)
              gTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttattt                                                                                                                                       <  2:3756633/150‑1 (MQ=255)
               ttGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGtttattta                                                                                                                                      <  1:492016/150‑1 (MQ=255)
               atGGTTCTTGCCAATTTTGATACCCGCATAGGCTTGCGGGCTCTCATTAACGTTACCCATGGCGGAAAACAGGTGCCGTTAGTATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGTGTGATACCGGGAAAGtttatttc                                                                                                                                      >  2:4627123/2‑149 (MQ=255)
                tGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTaa                                                                                                                                     >  1:492856/1‑150 (MQ=255)
                    tCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtgg                                                                                                                                 <  2:411970/150‑1 (MQ=255)
                     cGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggt                                                                                                                                <  1:4073130/150‑1 (MQ=255)
                      gTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggtg                                                                                                                               <  1:2138173/150‑1 (MQ=255)
                       tGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTgc                                                                                                                              <  2:4257909/150‑1 (MQ=255)
                          cAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGTCGGAAAACCGGTGCCGTTTTGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGcc                                                                                                                           <  2:634400/150‑1 (MQ=255)
                          cAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGcc                                                                                                                           >  1:3462221/1‑150 (MQ=255)
                            aTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCAt                                                                                                                         >  1:1446054/1‑150 (MQ=255)
                              tttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTg                                                                                                                       <  1:2606573/150‑1 (MQ=255)
                               ttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGt                                                                                                                      <  1:3329739/150‑1 (MQ=255)
                               ttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGt                                                                                                                      >  1:750969/1‑150 (MQ=255)
                                tGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTc                                                                                                                     >  2:1041133/1‑150 (MQ=255)
                                  aTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTg                                                                                                                   >  1:2922543/1‑150 (MQ=255)
                                  aTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACAAGTATGGTGGGTGATTACGGGCAAGTTTATATAAGTGGTGCGCCATTGTCTg                                                                                                                   >  1:614703/1‑150 (MQ=255)
                                    aCCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGt                                                                                                                 <  2:2391904/150‑1 (MQ=255)
                                     cccGCATAGGCGTGCGGGCGCTCATTACCGTGACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTg                                                                                                                <  2:4612263/150‑1 (MQ=255)
                                            aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACt                                                                                                         <  2:4434639/150‑1 (MQ=255)
                                             ggCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTg                                                                                                        <  2:3201501/150‑1 (MQ=255)
                                             ggCGTGCGGGCGCTCATTAACGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTAACGGAAAAACCAGTATGGTGGGTGATTACGGGCAAGGGTATTTAAGTGGTGCGCCCTTGTCGGGTGAATTACTg                                                                                                        >  1:2018677/1‑150 (MQ=255)
                                              gCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTgg                                                                                                       <  1:1702254/150‑1 (MQ=255)
                                                gTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGtt                                                                                                     >  1:4600/1‑150 (MQ=255)
                                                 tGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTc                                                                                                    >  2:948049/1‑150 (MQ=255)
                                                  gCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCa                                                                                                   <  2:2849300/150‑1 (MQ=255)
                                                       cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTggg                                                                                              >  2:1930608/1‑150 (MQ=255)
                                                       cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTggg                                                                                              >  2:131488/1‑150 (MQ=255)
                                                       cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTggg                                                                                              >  1:1924329/1‑150 (MQ=255)
                                                        gcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTgggg                                                                                             <  2:1619737/150‑1 (MQ=255)
                                                          tCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGgag                                                                                           >  1:1251335/1‑150 (MQ=255)
                                                           cATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGgaga                                                                                          <  1:1058021/150‑1 (MQ=255)
                                                            aTTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGAc                                                                                         >  2:3999524/1‑150 (MQ=255)
                                                                ccGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcg                                                                                     <  1:3327293/150‑1 (MQ=255)
                                                                 cGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcgc                                                                                    <  2:2934812/150‑1 (MQ=255)
                                                                 cGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcgc                                                                                    >  1:1791293/1‑150 (MQ=255)
                                                                 cGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcgc                                                                                    <  2:2817623/150‑1 (MQ=255)
                                                                 cGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcgc                                                                                    <  2:2677112/150‑1 (MQ=255)
                                                                  gTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGcgcg                                                                                   >  1:4373804/1‑150 (MQ=255)
                                                                     aCCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAAc                                                                                >  1:2543245/1‑150 (MQ=255)
                                                                     aCCCAGGGCGGAAAACCGGTGCCGATTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAAc                                                                                >  1:2330353/1‑150 (MQ=255)
                                                                         agtgCGGAAAAACGGTGCCGTTTGGATCACTGGTACGGTAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGTGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGTGAGACGGCGCGATCTCAc                                                                            <  2:1529263/147‑1 (MQ=255)
                                                                         aGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCAc                                                                            <  1:985209/150‑1 (MQ=255)
                                                                                 aaaCCGGTGCCGTTTGGATCACTGGTACGGTAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                    <  1:1103669/150‑1 (MQ=255)
                                                                                 aaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                    >  1:2061238/1‑150 (MQ=255)
                                                                                 aaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                    <  1:1492047/150‑1 (MQ=255)
                                                                                 aaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAtt                                                                    <  1:3861038/150‑1 (MQ=255)
                                                                                  aaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTg                                                                   <  1:4638875/150‑1 (MQ=255)
                                                                                     cGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTGATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTgcgc                                                                >  2:4317517/1‑150 (MQ=255)
                                                                                         gCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTa                                                            <  2:1120315/150‑1 (MQ=255)
                                                                                            gTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGt                                                         <  1:4354204/150‑1 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                       <  1:4312385/150‑1 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                       <  1:3567932/150‑1 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                       >  1:3836689/1‑150 (MQ=255)
                                                                                              ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAt                                                       <  2:3481740/150‑1 (MQ=255)
                                                                                               tGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAtt                                                      <  1:522976/150‑1 (MQ=255)
                                                                                                ggATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTg                                                     <  2:1067154/150‑1 (MQ=255)
                                                                                                 gATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGc                                                    <  1:4487667/150‑1 (MQ=255)
                                                                                                  aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                                   >  1:1229249/1‑150 (MQ=255)
                                                                                                   tCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCg                                                  <  1:1643046/150‑1 (MQ=255)
                                                                                                   tCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCg                                                  <  2:1347763/150‑1 (MQ=255)
                                                                                                    cACTGGTACGGGAAAACAGTACCGGACTAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGa                                                 <  1:1626057/150‑1 (MQ=255)
                                                                                                    cACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGa                                                 >  1:4521087/1‑150 (MQ=255)
                                                                                                     aCTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGaa                                                <  2:3422806/150‑1 (MQ=255)
                                                                                                      cTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAg                                               >  2:4331802/1‑150 (MQ=255)
                                                                                                        ggTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCa                                             <  2:2078818/150‑1 (MQ=255)
                                                                                                         gTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCaa                                            >  1:2663593/1‑150 (MQ=255)
                                                                                                           aCGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAg                                          <  1:3692370/150‑1 (MQ=255)
                                                                                                               gAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTa                                      >  2:2758183/1‑150 (MQ=255)
                                                                                                                 aaaCAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTAAAGTGGTGCGCCATTGTCTGGTGAATTACGGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACa                                    >  1:3756930/1‑150 (MQ=255)
                                                                                                                     aGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCaa                                <  2:4261606/150‑1 (MQ=255)
                                                                                                                       tACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGc                              <  1:306214/150‑1 (MQ=255)
                                                                                                                           ggAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTc                          <  1:2050146/150‑1 (MQ=255)
                                                                                                                            gAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCa                         <  2:1516681/150‑1 (MQ=255)
                                                                                                                              aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                       >  2:2757778/1‑150 (MQ=255)
                                                                                                                              aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                       >  1:4228410/1‑150 (MQ=255)
                                                                                                                               tAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTg                      >  2:1119132/1‑150 (MQ=255)
                                                                                                                                aaCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGt                     <  1:3953251/150‑1 (MQ=255)
                                                                                                                                aaCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGt                     <  1:3953337/150‑1 (MQ=255)
                                                                                                                                    aGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAtt                 <  1:815828/150‑1 (MQ=255)
                                                                                                                                      tATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTc               <  2:561544/150‑1 (MQ=255)
                                                                                                                                      tATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGATCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTc               >  2:1979527/1‑150 (MQ=255)
                                                                                                                                      tATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTc               >  1:152289/1‑150 (MQ=255)
                                                                                                                                         ggtgggtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGc            <  2:2100983/150‑1 (MQ=255)
                                                                                                                                            gggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGt         <  2:3611365/150‑1 (MQ=255)
                                                                                                                                              gtgATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGttt       <  2:2675829/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  CP009273/557445‑557741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  CP009273/557444‑557741
                                                                                                                                                    |                                                                                                                                                     
GCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTA                                                                                                                                                      >  SRR19973072.3076848/1‑150 (MQ=60)
 CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTAC                                                                                                                                                     <  SRR19973072.2591012/150‑1 (MQ=60)
  CGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACG                                                                                                                                                    >  SRR19973072.2681537/1‑150 (MQ=60)
   GACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGG                                                                                                                                                   >  SRR19973072.4579573/1‑150 (MQ=60)
   GACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGG                                                                                                                                                   >  SRR19973072.2784164/1‑150 (MQ=60)
   GACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGG                                                                                                                                                   >  SRR19973072.1569365/1‑150 (MQ=60)
     CGCAAGGCGCGTTGGTTCGTGTCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGC                                                                                                                                                 <  SRR19973072.394684/150‑1 (MQ=60)
     CGCAAGGCGCGTTGGTTCGTGTCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGC                                                                                                                                                 <  SRR19973072.394651/150‑1 (MQ=60)
     CGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGC                                                                                                                                                 <  SRR19973072.4116904/150‑1 (MQ=60)
       CAAGGCGCGTTGGTTCGTGCCAATTTTGCTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAA                                                                                                                                               <  SRR19973072.2588082/150‑1 (MQ=60)
       CAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAA                                                                                                                                               >  SRR19973072.2823659/1‑150 (MQ=60)
       CAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAA                                                                                                                                               <  SRR19973072.2215326/150‑1 (MQ=60)
       CAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAA                                                                                                                                               <  SRR19973072.2773595/150‑1 (MQ=60)
        AAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAG                                                                                                                                              >  SRR19973072.606850/1‑150 (MQ=60)
        AAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAG                                                                                                                                              >  SRR19973072.4499187/1‑150 (MQ=60)
               GTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTT                                                                                                                                       <  SRR19973072.1738011/150‑1 (MQ=60)
                TTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTA                                                                                                                                      >  SRR19973072.1272165/1‑150 (MQ=60)
                TTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTA                                                                                                                                      <  SRR19973072.493178/150‑1 (MQ=60)
                 TGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAA                                                                                                                                     >  SRR19973072.3758470/1‑150 (MQ=60)
                 TGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAA                                                                                                                                     >  SRR19973072.494034/1‑150 (MQ=60)
                 TGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAA                                                                                                                                     >  SRR19973072.494022/1‑150 (MQ=60)
                 TGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTGTATTTAA                                                                                                                                     >  SRR19973072.493900/1‑150 (MQ=60)
                   GTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGT                                                                                                                                   <  SRR19973072.1737233/150‑1 (MQ=60)
                      CGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGT                                                                                                                                <  SRR19973072.4081676/150‑1 (MQ=60)
                       GTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTG                                                                                                                               <  SRR19973072.2142833/150‑1 (MQ=60)
                           CAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCC                                                                                                                           >  SRR19973072.3469471/1‑150 (MQ=60)
                           CAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCC                                                                                                                           >  SRR19973072.1367099/1‑150 (MQ=60)
                           CAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCC                                                                                                                           >  SRR19973072.3067659/1‑150 (MQ=60)
                           AAATTTTGATACCCGCATAGGCGTGCGGGCGCTAATAACCGTTAACCAGGGAGGAAAACCTGTGACGTTTTGATCACTTTTACGGGAAAACATTACAGGAATAACCAGTATGGTGGGTGATTACGTGCAAGTATATTTAAGTGGTGCGCA                                                                                                                           >  SRR19973072.958216/1‑150 (MQ=60)
                             ATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCAT                                                                                                                         >  SRR19973072.1449169/1‑150 (MQ=60)
                               TTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTG                                                                                                                       <  SRR19973072.2612192/150‑1 (MQ=60)
                               TTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTG                                                                                                                       <  SRR19973072.2612207/150‑1 (MQ=60)
                                TTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGT                                                                                                                      >  SRR19973072.752616/1‑150 (MQ=60)
                                TTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGT                                                                                                                      <  SRR19973072.3336806/150‑1 (MQ=60)
                                TTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGT                                                                                                                      <  SRR19973072.3336485/150‑1 (MQ=60)
                                TTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGT                                                                                                                      >  SRR19973072.752566/1‑150 (MQ=60)
                                 TGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTC                                                                                                                     <  SRR19973072.1668436/150‑1 (MQ=60)
                                   ATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTG                                                                                                                   >  SRR19973072.2928796/1‑150 (MQ=60)
                                   ATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACAAGTATGGTGGGTGATTACGGGCAAGTTTATATAAGTGGTGCGCCATTGTCTG                                                                                                                   >  SRR19973072.616119/1‑150 (MQ=60)
                                              GGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTG                                                                                                        >  SRR19973072.2023089/1‑150 (MQ=60)
                                              GGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTG                                                                                                        >  SRR19973072.2023088/1‑150 (MQ=60)
                                              GGCGTGCGGGCGCTCATTAACGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTAACGGAAAAACCAGTATGGTGGGTGATTACGGGCAAGGGTATTTAAGTGGTGCGCCCTTGTCGGGTGAATTACTG                                                                                                        >  SRR19973072.2023039/1‑150 (MQ=60)
                                               GCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGG                                                                                                       <  SRR19973072.1705986/150‑1 (MQ=60)
                                                 GTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTT                                                                                                     >  SRR19973072.1519904/1‑150 (MQ=60)
                                                 GTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGGATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGTATTACTGGTT                                                                                                     >  SRR19973072.4613/1‑150 (MQ=60)
                                                     GGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGT                                                                                                 >  SRR19973072.3611966/1‑150 (MQ=60)
                                                      GGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTGTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTG                                                                                                >  SRR19973072.1870726/1‑150 (MQ=60)
                                                        CGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGG                                                                                              >  SRR19973072.1928415/1‑150 (MQ=60)
                                                           TCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAG                                                                                           >  SRR19973072.1254021/1‑150 (MQ=60)
                                                            CATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGA                                                                                          <  SRR19973072.1060291/150‑1 (MQ=60)
                                                                 CCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCG                                                                                     <  SRR19973072.3334526/150‑1 (MQ=60)
                                                                 CCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCG                                                                                     <  SRR19973072.3334360/150‑1 (MQ=60)
                                                                  CGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGC                                                                                    >  SRR19973072.4444186/1‑150 (MQ=60)
                                                                  CGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGC                                                                                    >  SRR19973072.1795220/1‑150 (MQ=60)
                                                                   GTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCG                                                                                   >  SRR19973072.4382994/1‑150 (MQ=60)
                                                                      ACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAAC                                                                                >  SRR19973072.2548769/1‑150 (MQ=60)
                                                                      ACCCAGGGCGGAAAACCGGTGCCGATTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAAC                                                                                >  SRR19973072.2335346/1‑150 (MQ=60)
                                                                         CAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCA                                                                             <  SRR19973072.469215/150‑1 (MQ=60)
                                                                          AGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCAC                                                                            <  SRR19973072.987361/150‑1 (MQ=60)
                                                                          AGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCAC                                                                            >  SRR19973072.1973652/1‑150 (MQ=60)
                                                                          AGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCAC                                                                            <  SRR19973072.254953/150‑1 (MQ=60)
                                                                            GGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGC                                                                          >  SRR19973072.706745/1‑150 (MQ=60)
                                                                                  AAACCGGTGCCGTTTGGATCACTGGTACGGTAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATT                                                                    <  SRR19973072.1106002/150‑1 (MQ=60)
                                                                                  AAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATT                                                                    <  SRR19973072.1495235/150‑1 (MQ=60)
                                                                                  AAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATT                                                                    >  SRR19973072.2065725/1‑150 (MQ=60)
                                                                                  AAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATT                                                                    <  SRR19973072.3869081/150‑1 (MQ=60)
                                                                                  AAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATT                                                                    <  SRR19973072.3869080/150‑1 (MQ=60)
                                                                                  AAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATT                                                                    <  SRR19973072.3869074/150‑1 (MQ=60)
                                                                                   AACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTG                                                                   <  SRR19973072.4648526/150‑1 (MQ=60)
                                                                                   AACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTG                                                                   <  SRR19973072.4648577/150‑1 (MQ=60)
                                                                                   AACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTG                                                                   <  SRR19973072.4648591/150‑1 (MQ=60)
                                                                                   AACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTG                                                                   <  SRR19973072.4100843/150‑1 (MQ=60)
                                                                                           CCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTAT                                                           <  SRR19973072.1089668/150‑1 (MQ=60)
                                                                                             GTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGT                                                         <  SRR19973072.4363337/150‑1 (MQ=60)
                                                                                               TTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAT                                                       >  SRR19973072.3844699/1‑150 (MQ=60)
                                                                                               TTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAT                                                       <  SRR19973072.4321394/150‑1 (MQ=60)
                                                                                               TTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAT                                                       <  SRR19973072.3575442/150‑1 (MQ=60)
                                                                                               TTGGATCACTGGTACGGGAAAACAGTACCGGAATAAACAGTATGGTGGGTGATTACGGTCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAT                                                       <  SRR19973072.4337351/150‑1 (MQ=60)
                                                                                                TGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATT                                                      <  SRR19973072.524224/150‑1 (MQ=60)
                                                                                                  GATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGC                                                    <  SRR19973072.4497139/150‑1 (MQ=60)
                                                                                                  GATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGC                                                    <  SRR19973072.3731079/150‑1 (MQ=60)
                                                                                                   ATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCC                                                   <  SRR19973072.2180380/150‑1 (MQ=60)
                                                                                                   ATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCC                                                   >  SRR19973072.4057552/1‑150 (MQ=60)
                                                                                                   ATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCC                                                   >  SRR19973072.1231877/1‑150 (MQ=60)
                                                                                                   ATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCC                                                   >  SRR19973072.1231910/1‑150 (MQ=60)
                                                                                                    TCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCG                                                  <  SRR19973072.1646631/150‑1 (MQ=60)
                                                                                                     CACTGGTACGGGAAAACAGTACCGGACTAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGA                                                 <  SRR19973072.1629592/150‑1 (MQ=60)
                                                                                                     CACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGA                                                 >  SRR19973072.4530630/1‑150 (MQ=60)
                                                                                                     CACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGA                                                 <  SRR19973072.1629510/150‑1 (MQ=60)
                                                                                                        TGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGC                                              >  SRR19973072.1486687/1‑150 (MQ=60)
                                                                                                          GTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAA                                            >  SRR19973072.2669295/1‑150 (MQ=60)
                                                                                                           TACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAA                                           <  SRR19973072.2291100/150‑1 (MQ=60)
                                                                                                           TACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAA                                           <  SRR19973072.118184/150‑1 (MQ=60)
                                                                                                           TACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAA                                           <  SRR19973072.2291090/150‑1 (MQ=60)
                                                                                                            ACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAG                                          <  SRR19973072.3700154/150‑1 (MQ=60)
                                                                                                                 AAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTAC                                     >  SRR19973072.900554/1‑150 (MQ=60)
                                                                                                                  AAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTAAAGTGGTGCGCCATTGTCTGGTGAATTACGGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACA                                    >  SRR19973072.3764825/1‑150 (MQ=60)
                                                                                                                       GTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAG                               >  SRR19973072.241672/1‑150 (MQ=60)
                                                                                                                        TACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGC                              <  SRR19973072.306923/150‑1 (MQ=60)
                                                                                                                         ACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCC                             <  SRR19973072.3863680/150‑1 (MQ=60)
                                                                                                                            GGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTC                          <  SRR19973072.2054607/150‑1 (MQ=60)
                                                                                                                            GGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTC                          <  SRR19973072.2054681/150‑1 (MQ=60)
                                                                                                                               ATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACT                       <  SRR19973072.2073728/150‑1 (MQ=60)
                                                                                                                               ATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACT                       >  SRR19973072.4237163/1‑150 (MQ=60)
                                                                                                                               ATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACT                       >  SRR19973072.4237226/1‑150 (MQ=60)
                                                                                                                                TAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTG                      <  SRR19973072.1635708/150‑1 (MQ=60)
                                                                                                                                TAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTG                      <  SRR19973072.3446523/150‑1 (MQ=60)
                                                                                                                                TAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTG                      <  SRR19973072.3810956/150‑1 (MQ=60)
                                                                                                                                 AACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGT                     >  SRR19973072.4023892/1‑150 (MQ=60)
                                                                                                                                 AACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGT                     <  SRR19973072.3961601/150‑1 (MQ=60)
                                                                                                                                 AACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGT                     <  SRR19973072.3961581/150‑1 (MQ=60)
                                                                                                                                 AACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGT                     <  SRR19973072.3961515/150‑1 (MQ=60)
                                                                                                                                    CAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAT                  <  SRR19973072.4257154/150‑1 (MQ=60)
                                                                                                                                    CAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAT                  <  SRR19973072.26006/150‑1 (MQ=60)
                                                                                                                                     AGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATT                 <  SRR19973072.817708/150‑1 (MQ=60)
                                                                                                                                     AGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATT                 <  SRR19973072.817581/150‑1 (MQ=60)
                                                                                                                                       TATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTC               >  SRR19973072.152679/1‑150 (MQ=60)
                                                                                                                                         TGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAATGCTTACAGCAAGCCGTCACTGTTATTTCGG             <  SRR19973072.4287397/150‑1 (MQ=60)
                                                                                                                                          GGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGC            >  SRR19973072.579801/1‑150 (MQ=60)
                                                                                                                                            TGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAG          >  SRR19973072.3232473/1‑150 (MQ=60)
                                                                                                                                               GTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTT       <  SRR19973072.3562715/150‑1 (MQ=60)
                                                                                                                                                 GATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGC     <  SRR19973072.4378791/150‑1 (MQ=60)
                                                                                                                                                   TTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCAC   <  SRR19973072.1909464/150‑1 (MQ=60)
                                                                                                                                                    GACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  <  SRR19973072.4400505/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  CP009273/557444‑557741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑