Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,592 G→T 100% D814Y (GAC→TAC)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,5920GT96.7% 661.0 / 12.1 214D814Y (GAC→TAC) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base G (1/6);  new base T (106/100);  total (107/107)
Fisher's exact test for biased strand distribution p-value = 6.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

GACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTT  >  CP009273/557447‑557735
                                                                                                                                                  |                                                                                                                                               
gACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTAc                                                                                                                                               <  2:2977663/148‑1 (MQ=255)
  cGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACgg                                                                                                                                             >  1:418100/1‑148 (MQ=255)
     aaGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCa                                                                                                                                          >  1:3456141/1‑148 (MQ=255)
      aGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                         <  1:2571336/148‑1 (MQ=255)
        gcgcGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGt                                                                                                                                       <  1:2737455/148‑1 (MQ=255)
        gcgcGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGt                                                                                                                                       <  1:3205498/148‑1 (MQ=255)
         cgcgTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGtt                                                                                                                                      <  1:2923339/148‑1 (MQ=255)
           cgTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGttta                                                                                                                                    <  1:624201/148‑1 (MQ=255)
             ttGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGtttatt                                                                                                                                  >  2:607785/1‑148 (MQ=255)
             ttGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGAAATtttatt                                                                                                                                  >  2:3026634/1‑148 (MQ=255)
              tGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTAAGGGCAAGtttattt                                                                                                                                 >  1:1276529/1‑148 (MQ=255)
              tGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGGGGGTGATTACGGGCAAGtttattt                                                                                                                                 >  1:1335285/1‑148 (MQ=255)
               ggTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGtttattta                                                                                                                                <  2:3417865/148‑1 (MQ=255)
                gTTCGTGCCAATTTTGAGACCCGCAGAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTaa                                                                                                                               <  2:2070562/148‑1 (MQ=255)
                 ttCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAg                                                                                                                              >  2:853789/1‑148 (MQ=255)
                 ttCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAg                                                                                                                              >  2:174018/1‑148 (MQ=255)
                  tCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCACGTTTATTTAAgt                                                                                                                             >  1:3151133/1‑148 (MQ=255)
                  tCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgt                                                                                                                             >  1:1996493/1‑148 (MQ=255)
                  tCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgt                                                                                                                             >  1:201334/1‑148 (MQ=255)
                  tCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgt                                                                                                                             <  1:2740910/148‑1 (MQ=255)
                   cGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtg                                                                                                                            <  2:2884547/148‑1 (MQ=255)
                     tGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgtggt                                                                                                                          <  2:3081645/148‑1 (MQ=255)
                          aTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGcc                                                                                                                     <  1:968284/148‑1 (MQ=255)
                           ttttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                    <  2:2332879/148‑1 (MQ=255)
                              tGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTg                                                                                                                 >  2:423785/1‑148 (MQ=255)
                                aTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTc                                                                                                               >  2:2620417/1‑148 (MQ=255)
                                  aCCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTg                                                                                                             >  2:1213360/1‑148 (MQ=255)
                                       cATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCTGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGaa                                                                                                        <  2:498497/148‑1 (MQ=255)
                                        aTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGcc                                                                                                                     >  1:3557763/1‑134 (MQ=255)
                                        aTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGcc                                                                                                                     <  2:3557758/134‑1 (MQ=255)
                                        aTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAAt                                                                                                       <  1:816698/148‑1 (MQ=255)
                                          aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                     <  1:1394198/148‑1 (MQ=255)
                                          aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AAAAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                     >  1:1638549/1‑148 (MQ=255)
                                          aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACAGGTACGGGAAAAAAGTAACGG‑AATAACCCGGATGGTGGGTGATTAAGGGCAAGTTTATTTAAGTGGTGCGCCCGTGTCTGGGGAATTa                                                                                                     >  1:1523096/1‑148 (MQ=255)
                                          aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGGGTGGATCACTGGTACGGGAAAACAGTACCGGAAATAACC‑GTATGGTGGGTGATTACGGGCCAGTTTATTTAAGTGGTGCGCCAGTGTCTGGTGGATTa                                                                                                     >  1:3296319/1‑148 (MQ=255)
                                          aGGCGTGCGGGCGCTCAGTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                     <  2:163193/148‑1 (MQ=255)
                                             cGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTg                                                                                                  <  2:632337/148‑1 (MQ=255)
                                                  gggCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCa                                                                                             <  1:2810712/148‑1 (MQ=255)
                                                   ggCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAg                                                                                            >  1:454687/1‑148 (MQ=255)
                                                    gcgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGt                                                                                           >  1:428359/1‑148 (MQ=255)
                                                     cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTg                                                                                          <  1:119636/148‑1 (MQ=255)
                                                     cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTg                                                                                          <  1:1714160/148‑1 (MQ=255)
                                                     cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTg                                                                                          >  2:2221097/1‑148 (MQ=255)
                                                     cgcTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTg                                                                                          >  2:3458391/1‑148 (MQ=255)
                                                     cgcTCATTACCGGTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTg                                                                                          <  1:1919863/148‑1 (MQ=255)
                                                        tCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTgggg                                                                                       >  1:720394/1‑148 (MQ=255)
                                                           ttACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGgaga                                                                                    >  1:3459311/1‑148 (MQ=255)
                                                              ccGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACgg                                                                                 >  2:1219005/1‑148 (MQ=255)
                                                              ccGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACgg                                                                                 >  2:2277340/1‑148 (MQ=255)
                                                                gTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGgcg                                                                               <  2:1319868/148‑1 (MQ=255)
                                                                gTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTATGGTGAATTACTGGTTCAGTGGGGAGACGgcg                                                                               <  1:845413/148‑1 (MQ=255)
                                                                          gCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACg                                                                     <  1:2785780/148‑1 (MQ=255)
                                                                          gCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACg                                                                     <  1:3414582/148‑1 (MQ=255)
                                                                             gAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGTCTAGTTTATTTAAGTGGTGCGCAAGTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGATCTCACGCTg                                                                  <  1:869977/148‑1 (MQ=255)
                                                                             gAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTg                                                                  >  1:1817689/1‑148 (MQ=255)
                                                                             gAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAGTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTg                                                                  <  2:2141708/148‑1 (MQ=255)
                                                                                aaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAt                                                               >  2:384768/1‑148 (MQ=255)
                                                                                    ggTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTgcg                                                           <  1:1791642/148‑1 (MQ=255)
                                                                                     gTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTgcgc                                                          <  2:558452/148‑1 (MQ=255)
                                                                                     gTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTgcgc                                                          <  2:739637/148‑1 (MQ=255)
                                                                                     gTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTgcgc                                                          <  1:2208176/148‑1 (MQ=255)
                                                                                      tGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                         <  2:542030/148‑1 (MQ=255)
                                                                                      tGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                         <  1:182509/148‑1 (MQ=255)
                                                                                          gTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTAt                                                     <  2:1989906/148‑1 (MQ=255)
                                                                                          gTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTAt                                                     <  2:716238/148‑1 (MQ=255)
                                                                                             tGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTa                                                  <  1:2488614/148‑1 (MQ=255)
                                                                                               gATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAtt                                                >  1:1553264/1‑148 (MQ=255)
                                                                                               gATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAtt                                                >  2:3375449/1‑148 (MQ=255)
                                                                                                  cACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                             <  1:2145417/148‑1 (MQ=255)
                                                                                                  cACGGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                             <  2:3286984/148‑1 (MQ=255)
                                                                                                   aCTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCg                                            >  2:1414956/1‑148 (MQ=255)
                                                                                                      ggTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAg                                         <  1:523770/148‑1 (MQ=255)
                                                                                                        tACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCa                                       >  1:205059/1‑148 (MQ=255)
                                                                                                          cGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCaaa                                     >  2:1552981/1‑148 (MQ=255)
                                                                                                           gggAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGAT‑ACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGc                                   <  2:319814/148‑1 (MQ=255)
                                                                                                            ggAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGc                                   >  2:410329/1‑148 (MQ=255)
                                                                                                               aaaCAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTa                                >  2:3002980/1‑148 (MQ=255)
                                                                                                               aaaCAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTa                                <  2:201343/148‑1 (MQ=255)
                                                                                                                aaCAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTAc                               >  1:2175844/1‑148 (MQ=255)
                                                                                                                 aCAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACa                              >  2:2949467/1‑148 (MQ=255)
                                                                                                                     tACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCaa                          >  1:1676732/1‑148 (MQ=255)
                                                                                                                     tACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCaa                          >  1:1079050/1‑148 (MQ=255)
                                                                                                                      aCCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAg                         <  2:1504837/148‑1 (MQ=255)
                                                                                                                       ccGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGc                        <  1:2769436/148‑1 (MQ=255)
                                                                                                                        cGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGcc                       >  1:2849723/1‑148 (MQ=255)
                                                                                                                            aaTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTc                    >  2:837611/1‑148 (MQ=255)
                                                                                                                              tAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAc                  >  1:1417373/1‑148 (MQ=255)
                                                                                                                              tAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAc                  <  2:2646234/148‑1 (MQ=255)
                                                                                                                              tAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAc                  <  1:3525720/148‑1 (MQ=255)
                                                                                                                              tAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAc                  >  2:717602/1‑148 (MQ=255)
                                                                                                                                  cAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGtt              >  2:2575235/1‑148 (MQ=255)
                                                                                                                                   aGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTa             >  1:2454737/1‑148 (MQ=255)
                                                                                                                                    gTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAt            <  2:1819398/148‑1 (MQ=255)
                                                                                                                                     tATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAtt           <  1:3276129/148‑1 (MQ=255)
                                                                                                                                     tATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAtt           >  2:276392/1‑148 (MQ=255)
                                                                                                                                            ggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGTCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAg    >  1:1758992/1‑148 (MQ=255)
                                                                                                                                             gtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGt   <  1:795805/148‑1 (MQ=255)
                                                                                                                                             gtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGt   <  1:2579752/148‑1 (MQ=255)
                                                                                                                                              tgattaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGtt  <  1:1585446/148‑1 (MQ=255)
                                                                                                                                              tgattaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGtt  >  2:1041461/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTT  >  CP009273/557447‑557735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  CP009273/557446‑557741
                                                                                                                                                   |                                                                                                                                                     
CGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACG                                                                                                                                                    <  SRR19973067.2167622/150‑1 (MQ=60)
CGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACG                                                                                                                                                    <  SRR19973067.2167297/150‑1 (MQ=60)
CGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACG                                                                                                                                                    <  SRR19973067.2782152/150‑1 (MQ=60)
 GACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGG                                                                                                                                                   >  SRR19973067.421003/1‑150 (MQ=60)
    GCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCA                                                                                                                                                >  SRR19973067.3478122/1‑150 (MQ=60)
       AGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGT                                                                                                                                             <  SRR19973067.3290436/150‑1 (MQ=60)
       AGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGT                                                                                                                                             <  SRR19973067.2588112/150‑1 (MQ=60)
         GCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTT                                                                                                                                           <  SRR19973067.2755137/150‑1 (MQ=60)
         GCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTT                                                                                                                                           <  SRR19973067.3225707/150‑1 (MQ=60)
          CGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTA                                                                                                                                          <  SRR19973067.2941918/150‑1 (MQ=60)
            CGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATT                                                                                                                                        <  SRR19973067.628324/150‑1 (MQ=60)
             GTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTT                                                                                                                                       >  SRR19973067.1343635/1‑150 (MQ=60)
             GTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTAAGGGCAAGTTTATTT                                                                                                                                       >  SRR19973067.1285221/1‑150 (MQ=60)
             GTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGGGGGTGATTACGGGCAAGTTTATTT                                                                                                                                       >  SRR19973067.1344403/1‑150 (MQ=60)
                 GTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCCAGTTTATTTAAGT                                                                                                                                   >  SRR19973067.202870/1‑150 (MQ=60)
                 GTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCACGTTTATTTAAGT                                                                                                                                   >  SRR19973067.3171114/1‑150 (MQ=60)
                 GTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGT                                                                                                                                   >  SRR19973067.2009973/1‑150 (MQ=60)
                 GTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGT                                                                                                                                   >  SRR19973067.202879/1‑150 (MQ=60)
                  TTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTG                                                                                                                                  >  SRR19973067.1637327/1‑150 (MQ=60)
                   TCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGG                                                                                                                                 <  SRR19973067.2758619/150‑1 (MQ=60)
                      TGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGC                                                                                                                              <  SRR19973067.1123977/150‑1 (MQ=60)
                           ATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCAT                                                                                                                         <  SRR19973067.974919/150‑1 (MQ=60)
                               TGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTC                                                                                                                     <  SRR19973067.2433102/150‑1 (MQ=60)
                                 ATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCCAGTTTATTTAAGTGGTGCGCCATTGTCTG                                                                                                                   >  SRR19973067.452460/1‑150 (MQ=60)
                                       GCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATagatcggaagag                                                                                                             >  SRR19973067.3580175/1‑138 (MQ=60)
                                       GCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATagatcggaagag                                                                                                             >  SRR19973067.3580170/1‑138 (MQ=60)
                                       GCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAAT                                                                                                             <  SRR19973067.2220965/150‑1 (MQ=60)
                                         ATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTA                                                                                                           <  SRR19973067.3447463/150‑1 (MQ=60)
                                         ATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTA                                                                                                           <  SRR19973067.822293/150‑1 (MQ=60)
                                         ATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AAAAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTA                                                                                                           >  SRR19973067.1649825/1‑150 (MQ=60)
                                         ATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACAGGTACGGGAAAAAAGTAACGG‑AATAACCCGGATGGTGGGTGATTAAGGGCAAGTTTATTTAAGTGGTGCGCCCGTGTCTGGGGAATTA                                                                                                           >  SRR19973067.1533321/1‑150 (MQ=60)
                                         ATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGGGTGGATCACTGGTACGGGAAAACAGTACCGGAAATAACC‑GTATGGTGGGTGATTACGGGCCAGTTTATTTAAGTGGTGCGCCAGTGTCTGGTGGATTA                                                                                                           >  SRR19973067.3317070/1‑150 (MQ=60)
                                           AGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACT                                                                                                         <  SRR19973067.1573419/150‑1 (MQ=60)
                                           AGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACT                                                                                                         <  SRR19973067.1403587/150‑1 (MQ=60)
                                           AGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACT                                                                                                         <  SRR19973067.1403669/150‑1 (MQ=60)
                                           AGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACT                                                                                                         <  SRR19973067.1403683/150‑1 (MQ=60)
                                           AGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTAAGGGAAAAAAGTACCGG‑AAAAAACACTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACT                                                                                                         >  SRR19973067.1235130/1‑150 (MQ=60)
                                                  CGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAG                                                                                                  >  SRR19973067.1897483/1‑150 (MQ=60)
                                                  CGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAG                                                                                                  >  SRR19973067.457837/1‑150 (MQ=60)
                                                   GGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGT                                                                                                 <  SRR19973067.2828579/150‑1 (MQ=60)
                                                   GGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGT                                                                                                 >  SRR19973067.431334/1‑150 (MQ=60)
                                                   GGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGT                                                                                                 <  SRR19973067.2828826/150‑1 (MQ=60)
                                                   GGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGT                                                                                                 >  SRR19973067.431201/1‑150 (MQ=60)
                                                     GCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGG                                                                                               <  SRR19973067.660982/150‑1 (MQ=60)
                                                      CGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGG                                                                                              <  SRR19973067.1725913/150‑1 (MQ=60)
                                                      CGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGG                                                                                              <  SRR19973067.120769/150‑1 (MQ=60)
                                                      CGCTCATTACCGGTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGG                                                                                              <  SRR19973067.1932963/150‑1 (MQ=60)
                                                       GCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGG                                                                                             >  SRR19973067.725395/1‑150 (MQ=60)
                                                         TCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAG                                                                                           >  SRR19973067.2693670/1‑150 (MQ=60)
                                                          CATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCCAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGA                                                                                          >  SRR19973067.3481306/1‑150 (MQ=60)
                                                          CATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGA                                                                                          >  SRR19973067.217688/1‑150 (MQ=60)
                                                          CATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGA                                                                                          >  SRR19973067.3481305/1‑150 (MQ=60)
                                                          CATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGA                                                                                          <  SRR19973067.1689702/150‑1 (MQ=60)
                                                            TTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGGTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGGGGGGAGACG                                                                                        >  SRR19973067.3303229/1‑150 (MQ=60)
                                                               CCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCG                                                                                     <  SRR19973067.734846/150‑1 (MQ=60)
                                                                 GTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTATGGTGAATTACTGGTTCAGTGGGGAGACGGCGCG                                                                                   <  SRR19973067.851054/150‑1 (MQ=60)
                                                                 GTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTATGGTGAATTACTGGTTCAGTGGGGAGACGGCGCG                                                                                   <  SRR19973067.851141/150‑1 (MQ=60)
                                                                        AGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCAC                                                                            >  SRR19973067.1727053/1‑150 (MQ=60)
                                                                           GCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCT                                                                         <  SRR19973067.2803755/150‑1 (MQ=60)
                                                                           GCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCT                                                                         <  SRR19973067.1665887/150‑1 (MQ=60)
                                                                           GCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCT                                                                         <  SRR19973067.2803376/150‑1 (MQ=60)
                                                                           GCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCT                                                                         <  SRR19973067.2803297/150‑1 (MQ=60)
                                                                           GCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCT                                                                         <  SRR19973067.3436318/150‑1 (MQ=60)
                                                                            CGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCCTTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTG                                                                        >  SRR19973067.1829992/1‑150 (MQ=60)
                                                                            CGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTG                                                                        >  SRR19973067.1829935/1‑150 (MQ=60)
                                                                              GAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGTCTAGTTTATTTAAGTGGTGCGCAAGTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGATCTCACGCTGCA                                                                      <  SRR19973067.875791/150‑1 (MQ=60)
                                                                                     GGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCA                                                               <  SRR19973067.1803714/150‑1 (MQ=60)
                                                                                      GTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCAC                                                              <  SRR19973067.2222900/150‑1 (MQ=60)
                                                                                       TGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACT                                                             <  SRR19973067.183847/150‑1 (MQ=60)
                                                                                       TGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACT                                                             <  SRR19973067.183956/150‑1 (MQ=60)
                                                                                           GTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGT                                                         >  SRR19973067.1762818/1‑150 (MQ=60)
                                                                                           GTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGT                                                         >  SRR19973067.695781/1‑150 (MQ=60)
                                                                                           GTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGAGCACTATGT                                                         >  SRR19973067.3406135/1‑150 (MQ=60)
                                                                                             TTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTAT                                                       <  SRR19973067.978202/150‑1 (MQ=60)
                                                                                              TGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATT                                                      <  SRR19973067.2504973/150‑1 (MQ=60)
                                                                                              TGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATT                                                      >  SRR19973067.1563846/1‑150 (MQ=60)
                                                                                              TGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATT                                                      >  SRR19973067.1563017/1‑150 (MQ=60)
                                                                                              TGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATT                                                      <  SRR19973067.2504954/150‑1 (MQ=60)
                                                                                                 ATCACTTGTACGGGAAAAGAGTACCGG‑TATAATCAGTATGGTGGGTGTTTACGGGCTAGTTTATTTAAGTGGTTCGCCATTGTCTGGTGAATTACTGGTTCTGTGGGGAGACGTAGCTTACTCACGCTGCATTGCGGACTATGTATTGCC                                                   <  SRR19973067.1512144/150‑1 (MQ=60)
                                                                                                   CACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGA                                                 <  SRR19973067.2159762/150‑1 (MQ=60)
                                                                                                     CTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAG                                               <  SRR19973067.87796/150‑1 (MQ=60)
                                                                                                       GGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGTGCACTATGTATTGCCGAAGCA                                             >  SRR19973067.929219/1‑150 (MQ=60)
                                                                                                       GGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGTGCACTATGTATTGCCGAAGCA                                             >  SRR19973067.929257/1‑150 (MQ=60)
                                                                                                       GGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCA                                             >  SRR19973067.207529/1‑150 (MQ=60)
                                                                                                       GGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCA                                             >  SRR19973067.206608/1‑150 (MQ=60)
                                                                                                       GGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCA                                             <  SRR19973067.527301/150‑1 (MQ=60)
                                                                                                       GGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCA                                             <  SRR19973067.532323/150‑1 (MQ=60)
                                                                                                          ACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAG                                          <  SRR19973067.3177908/150‑1 (MQ=60)
                                                                                                            GGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCT                                        <  SRR19973067.153076/150‑1 (MQ=60)
                                                                                                             GGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAAGTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTT                                       <  SRR19973067.2938935/150‑1 (MQ=60)
                                                                                                               AAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTAC                                     >  SRR19973067.2190391/1‑150 (MQ=60)
                                                                                                                    AGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAA                                >  SRR19973067.458931/1‑150 (MQ=60)
                                                                                                                    AGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAA                                >  SRR19973067.1086442/1‑150 (MQ=60)
                                                                                                                    AGTACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAA                                >  SRR19973067.1688290/1‑150 (MQ=60)
                                                                                                                       ACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCC                             >  SRR19973067.2868001/1‑150 (MQ=60)
                                                                                                                       ACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCC                             >  SRR19973067.2867958/1‑150 (MQ=60)
                                                                                                                       ACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCC                             >  SRR19973067.3562101/1‑150 (MQ=60)
                                                                                                                       ACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCC                             >  SRR19973067.2019673/1‑150 (MQ=60)
                                                                                                                       ACCGG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCC                             >  SRR19973067.2019880/1‑150 (MQ=60)
                                                                                                                        CCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCG                            <  SRR19973067.2787329/150‑1 (MQ=60)
                                                                                                                          GG‑AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTC                          >  SRR19973067.2582742/1‑150 (MQ=60)
                                                                                                                             AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAC                        >  SRR19973067.1426959/1‑150 (MQ=60)
                                                                                                                             AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAC                        >  SRR19973067.1801109/1‑150 (MQ=60)
                                                                                                                               TAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTG                      >  SRR19973067.276377/1‑150 (MQ=60)
                                                                                                                               TAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTG                      >  SRR19973067.275758/1‑150 (MQ=60)
                                                                                                                               TAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTG                      <  SRR19973067.3548022/150‑1 (MQ=60)
                                                                                                                                  CCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTA                   >  SRR19973067.2470911/1‑150 (MQ=60)
                                                                                                                                  CCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTA                   >  SRR19973067.2470874/1‑150 (MQ=60)
                                                                                                                                    AGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATT                 >  SRR19973067.2962144/1‑150 (MQ=60)
                                                                                                                                      TATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTC               <  SRR19973067.3296638/150‑1 (MQ=60)
                                                                                                                                      TATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTC               <  SRR19973067.3296635/150‑1 (MQ=60)
                                                                                                                                       ATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCG              <  SRR19973067.2009487/150‑1 (MQ=60)
                                                                                                                                       ATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCG              <  SRR19973067.2590059/150‑1 (MQ=60)
                                                                                                                                        TGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGG             <  SRR19973067.3480380/150‑1 (MQ=60)
                                                                                                                                           TGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGTCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAG          >  SRR19973067.1770969/1‑150 (MQ=60)
                                                                                                                                            GGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGT         <  SRR19973067.413186/150‑1 (MQ=60)
                                                                                                                                              GTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTT       <  SRR19973067.2596561/150‑1 (MQ=60)
                                                                                                                                              GTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTT       <  SRR19973067.801933/150‑1 (MQ=60)
                                                                                                                                              GTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTT       <  SRR19973067.801292/150‑1 (MQ=60)
                                                                                                                                               TGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTG      <  SRR19973067.1596304/150‑1 (MQ=60)
                                                                                                                                                  TTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCAC   <  SRR19973067.507278/150‑1 (MQ=60)
                                                                                                                                                   TACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  SRR19973067.1828621/1‑150 (MQ=60)
                                                                                                                                                   TACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  SRR19973067.1828513/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGG‑AATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACA  >  CP009273/557446‑557741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑