Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,592 G→T 100% D814Y (GAC→TAC)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,5920GT98.2% 689.7 / 3.3 219D814Y (GAC→TAC) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (100/115);  total (102/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 221 total aligned reads displayed.

GACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTT  >  CP009273/557447‑557736
                                                                                                                                                 |                                                                                                                                                
 aCGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACg                                                                                                                                               <  1:3483245/148‑1 (MQ=255)
     aaGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCa                                                                                                                                           >  1:2551757/1‑148 (MQ=255)
      aGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCaa                                                                                                                                          <  1:854252/148‑1 (MQ=255)
        gcgcGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGt                                                                                                                                        <  2:203861/148‑1 (MQ=255)
        gcgcGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGt                                                                                                                                        <  2:1771603/148‑1 (MQ=255)
        gcgcGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGt                                                                                                                                        <  2:711313/148‑1 (MQ=255)
        gcgcGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGt                                                                                                                                        <  1:41966/148‑1 (MQ=255)
          gcgTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGttt                                                                                                                                      <  1:1409383/148‑1 (MQ=255)
          gcgTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGttt                                                                                                                                      <  1:198224/148‑1 (MQ=255)
            gTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttat                                                                                                                                    <  1:3167698/148‑1 (MQ=255)
             ttGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttatt                                                                                                                                   >  1:2628997/1‑148 (MQ=255)
             ttGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttatt                                                                                                                                   <  1:901682/148‑1 (MQ=255)
             ttGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttatt                                                                                                                                   <  1:2762058/148‑1 (MQ=255)
               ggTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttattta                                                                                                                                 <  2:558033/148‑1 (MQ=255)
               ggTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGtttattta                                                                                                                                 <  1:1472821/148‑1 (MQ=255)
                gTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTaa                                                                                                                                <  2:1956753/148‑1 (MQ=255)
                gTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTaa                                                                                                                                >  2:2377187/1‑148 (MQ=255)
                gTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTaa                                                                                                                                <  1:2493328/148‑1 (MQ=255)
                  tCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAgt                                                                                                                              >  2:3433965/1‑148 (MQ=255)
                       ccAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGGTTACGGGCAAGTTTATTTTAgtggtgg                                                                                                                         >  1:3233890/1‑147 (MQ=255)
                          aTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTCGGGTGGGTGATTACGGGCCAGTTTATTGAAGTGGTGCGcc                                                                                                                      >  2:2163407/1‑148 (MQ=255)
                          aTTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGcc                                                                                                                      >  2:2163425/1‑148 (MQ=255)
                           ttttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                     >  1:2828559/1‑148 (MQ=255)
                           ttttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                     >  1:2827039/1‑148 (MQ=255)
                           ttttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                     >  1:2827059/1‑148 (MQ=255)
                           ttttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                     >  1:1830911/1‑148 (MQ=255)
                           ttttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                     <  1:1955214/148‑1 (MQ=255)
                           ttttGAGACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCa                                                                                                                     <  2:2396181/148‑1 (MQ=255)
                             ttGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAtt                                                                                                                   <  2:453951/148‑1 (MQ=255)
                               gATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGt                                                                                                                 <  1:486998/148‑1 (MQ=255)
                               gATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGt                                                                                                                 <  1:426476/148‑1 (MQ=255)
                                aTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTc                                                                                                                <  2:248652/148‑1 (MQ=255)
                                aTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTc                                                                                                                <  2:3489285/148‑1 (MQ=255)
                                aTACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTc                                                                                                                >  2:2579790/1‑148 (MQ=255)
                                 tACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCt                                                                                                               <  1:1802773/148‑1 (MQ=255)
                                   cccGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTgg                                                                                                             >  2:2281800/1‑148 (MQ=255)
                                    ccGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGt                                                                                                            >  1:182106/1‑148 (MQ=255)
                                    ccGCATAGGCGTGCGGGCGCTCATTAACGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACTGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGt                                                                                                            >  2:3416112/1‑148 (MQ=255)
                                      gCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGTAGTAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTAACGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGa                                                                                                          <  2:2759041/148‑1 (MQ=255)
                                        aTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAAt                                                                                                        <  2:40916/148‑1 (MQ=255)
                                         tAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAAtt                                                                                                       >  2:2895942/1‑148 (MQ=255)
                                          aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                      >  2:3376434/1‑148 (MQ=255)
                                          aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTa                                                                                                      >  2:910785/1‑148 (MQ=255)
                                          aGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTAACGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAATTGGTGCGCCAGTGTCTGGTGAATTa                                                                                                      >  2:2710872/1‑148 (MQ=255)
                                           ggCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTAc                                                                                                     >  2:729319/1‑148 (MQ=255)
                                           ggCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACAGGTAAGGGAAAACAGTACCGGAATAACCAGTATGGTTGGTGATTACGGGCAACTTTATTTAAATGGTGCGCCATTGTCTGGTGAAATaa                                                                                                     >  1:596144/1‑147 (MQ=255)
                                                gCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGtt                                                                                                <  2:1167025/148‑1 (MQ=255)
                                                  gggCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTTAATTACTGGTTCa                                                                                              <  1:3383092/148‑1 (MQ=255)
                                                  gggCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCa                                                                                              <  1:1217989/148‑1 (MQ=255)
                                                   ggCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAg                                                                                             <  2:2996494/148‑1 (MQ=255)
                                                         cATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGga                                                                                       >  1:3272299/1‑148 (MQ=255)
                                                             aCCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACg                                                                                   <  2:1087833/148‑1 (MQ=255)
                                                                  tACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGcgcg                                                                              >  1:2767906/1‑148 (MQ=255)
                                                                      cAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACt                                                                          <  2:846965/148‑1 (MQ=255)
                                                                       aGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAAGAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTTGTTCAGTGGGGAGACGGCGCGAACTc                                                                         <  2:3324791/148‑1 (MQ=255)
                                                                        gggCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCa                                                                        >  1:1343716/1‑148 (MQ=255)
                                                                          gCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACg                                                                      <  2:182106/148‑1 (MQ=255)
                                                                             gAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTg                                                                   <  2:3074902/148‑1 (MQ=255)
                                                                              aaaaCCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGc                                                                  <  1:2646765/148‑1 (MQ=255)
                                                                                  ccGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTg                                                              >  2:1876391/1‑148 (MQ=255)
                                                                                  ccGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTg                                                              >  1:2359265/1‑148 (MQ=255)
                                                                                      tGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                          <  2:2881818/148‑1 (MQ=255)
                                                                                      tGCCGTTTGGATCACTGGTAAGGGAAAACAGTACCGGAAAAAACAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTAATGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCa                                                          >  1:2682310/1‑148 (MQ=255)
                                                                                       gCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCAc                                                         >  2:2597107/1‑148 (MQ=255)
                                                                                       gCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCAc                                                         >  2:659609/1‑148 (MQ=255)
                                                                                         cGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTa                                                       >  1:165390/1‑148 (MQ=255)
                                                                                          gTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTAt                                                      >  1:431956/1‑148 (MQ=255)
                                                                                            ttGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGt                                                    <  2:2774489/148‑1 (MQ=255)
                                                                                                aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTg                                                <  2:3128679/148‑1 (MQ=255)
                                                                                                aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTg                                                <  2:2827040/148‑1 (MQ=255)
                                                                                                aTCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTg                                                <  2:2828559/148‑1 (MQ=255)
                                                                                                  cACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGcc                                              <  2:2373937/148‑1 (MQ=255)
                                                                                                   aCTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCAATATGTATTGCCg                                             <  1:2579353/148‑1 (MQ=255)
                                                                                                           gggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAg                                     <  2:2628997/148‑1 (MQ=255)
                                                                                                            ggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGc                                    >  1:1716316/1‑148 (MQ=255)
                                                                                                            ggAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGc                                    >  2:1027321/1‑148 (MQ=255)
                                                                                                             gAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCt                                   <  2:680555/148‑1 (MQ=255)
                                                                                                             gAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCt                                   <  2:289185/148‑1 (MQ=255)
                                                                                                              aaaaCAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCtt                                  >  2:3291862/1‑148 (MQ=255)
                                                                                                               aaaCAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTa                                 <  1:1893963/148‑1 (MQ=255)
                                                                                                                aaCAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTAc                                >  2:1899634/1‑148 (MQ=255)
                                                                                                                 aCAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACa                               >  1:1001404/1‑148 (MQ=255)
                                                                                                                  cAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAg                              <  2:1178086/148‑1 (MQ=255)
                                                                                                                  cAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAg                              >  2:2740265/1‑148 (MQ=255)
                                                                                                                     tACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCaa                           >  2:2544820/1‑148 (MQ=255)
                                                                                                                      aCCGGAATAACCAGTATGGTGGGTGATTACGGTCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAg                          <  1:1137721/148‑1 (MQ=255)
                                                                                                                      aCCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAg                          <  2:1236842/148‑1 (MQ=255)
                                                                                                                      aCCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAg                          <  2:545799/148‑1 (MQ=255)
                                                                                                                            aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCa                    <  2:2551807/148‑1 (MQ=255)
                                                                                                                            aTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCa                    <  1:3115307/148‑1 (MQ=255)
                                                                                                                             tAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAc                   >  1:1532779/1‑148 (MQ=255)
                                                                                                                              aaCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACt                  >  1:2616304/1‑148 (MQ=255)
                                                                                                                              aaCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATAGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAATCTTACAGCAAGCCGTCACt                  >  1:2987527/1‑148 (MQ=255)
                                                                                                                               aCCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTg                 <  2:139161/148‑1 (MQ=255)
                                                                                                                                ccAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGt                >  1:380791/1‑148 (MQ=255)
                                                                                                                                ccAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGt                <  1:968576/148‑1 (MQ=255)
                                                                                                                                    tATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTAtt            >  1:3493146/1‑148 (MQ=255)
                                                                                                                                       ggtgggtgATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCg         >  1:1513450/1‑148 (MQ=255)
                                                                                                                                             tgattaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGtt   <  1:2921622/148‑1 (MQ=255)
                                                                                                                                              gattaCGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGttt  >  2:209822/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTT  >  CP009273/557447‑557736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCAC  >  CP009273/557444‑557740
                                                                                                                                                    |                                                                                                                                                    
GCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTA                                                                                                                                                     <  SRR19973065.3374900/150‑1 (MQ=60)
GCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTA                                                                                                                                                     <  SRR19973065.567758/150‑1 (MQ=60)
GCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTA                                                                                                                                                     <  SRR19973065.1624376/150‑1 (MQ=60)
 CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTAC                                                                                                                                                    <  SRR19973065.2437853/150‑1 (MQ=60)
 CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTAC                                                                                                                                                    <  SRR19973065.97074/150‑1 (MQ=60)
 CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTAC                                                                                                                                                    <  SRR19973065.2501655/150‑1 (MQ=60)
 CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTAC                                                                                                                                                    <  SRR19973065.668571/150‑1 (MQ=60)
 CCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCATTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTAC                                                                                                                                                    <  SRR19973065.2997382/150‑1 (MQ=60)
  CGACGCTAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACG                                                                                                                                                   <  SRR19973065.1398748/150‑1 (MQ=60)
  CGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACG                                                                                                                                                   <  SRR19973065.1398784/150‑1 (MQ=60)
    ACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGG                                                                                                                                                 <  SRR19973065.3504442/150‑1 (MQ=60)
    ACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGG                                                                                                                                                 <  SRR19973065.3504438/150‑1 (MQ=60)
      GCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCA                                                                                                                                               >  SRR19973065.2567727/1‑150 (MQ=60)
      GCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCA                                                                                                                                               >  SRR19973065.2567777/1‑150 (MQ=60)
       CAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAA                                                                                                                                              >  SRR19973065.3147723/1‑150 (MQ=60)
         AGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGT                                                                                                                                            <  SRR19973065.859777/150‑1 (MQ=60)
           GGGCGTTGGTTCGTGCCTATTTTGATTCCCGCAGAGGCGTGCGGGCGCTCATTACCGTTAGCCAGGGCGGAAAACCGGTGCCGTTTGTTTCACTGGTTCGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTT                                                                                                                                          <  SRR19973065.2912395/150‑1 (MQ=60)
           GCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTT                                                                                                                                          <  SRR19973065.1151721/150‑1 (MQ=60)
           GCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTT                                                                                                                                          <  SRR19973065.42480/150‑1 (MQ=60)
             GCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTAT                                                                                                                                        <  SRR19973065.1418445/150‑1 (MQ=60)
             GCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTAT                                                                                                                                        <  SRR19973065.199762/150‑1 (MQ=60)
             GCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTAT                                                                                                                                        <  SRR19973065.199207/150‑1 (MQ=60)
             GCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTAT                                                                                                                                        <  SRR19973065.1418603/150‑1 (MQ=60)
             GCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTAT                                                                                                                                        <  SRR19973065.14650/150‑1 (MQ=60)
              CGTTGGTTCGTGCCAATTTTGATTCCCGCTTAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTTCCGTTTTGATCACTGGTTCGTGAAAACAGTACCGGACTAACCAGTATGGTGGGTGATTACGGGCAAGTTTATT                                                                                                                                       <  SRR19973065.3267716/150‑1 (MQ=60)
              CGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGTAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATT                                                                                                                                       <  SRR19973065.3268452/150‑1 (MQ=60)
              CGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATG                                                                                                                                       >  SRR19973065.2645257/1‑150 (MQ=60)
               GTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTT                                                                                                                                      <  SRR19973065.3186880/150‑1 (MQ=60)
                TTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTA                                                                                                                                     <  SRR19973065.907474/150‑1 (MQ=60)
                TTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTA                                                                                                                                     <  SRR19973065.3018002/150‑1 (MQ=60)
                TTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTA                                                                                                                                     <  SRR19973065.1971724/150‑1 (MQ=60)
                TTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTA                                                                                                                                     <  SRR19973065.2779220/150‑1 (MQ=60)
                  GGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAG                                                                                                                                   <  SRR19973065.576766/150‑1 (MQ=60)
                  GGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAG                                                                                                                                   <  SRR19973065.576843/150‑1 (MQ=60)
                  GGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAG                                                                                                                                   <  SRR19973065.1482253/150‑1 (MQ=60)
                  GGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAG                                                                                                                                   <  SRR19973065.2454327/150‑1 (MQ=60)
                   GTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGT                                                                                                                                  <  SRR19973065.2509016/150‑1 (MQ=60)
                   GTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGT                                                                                                                                  <  SRR19973065.980235/150‑1 (MQ=60)
                      CGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTCTTTAAGTGGT                                                                                                                               >  SRR19973065.397347/1‑150 (MQ=60)
                      CGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGT                                                                                                                               <  SRR19973065.800752/150‑1 (MQ=60)
                      CGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGT                                                                                                                               <  SRR19973065.1537037/150‑1 (MQ=60)
                        TGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGGTTACGGGCAAGTTTATTTTAGTGGTGG                                                                                                                             >  SRR19973065.3253337/1‑150 (MQ=60)
                            AATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCCAGTTTATTTAAGTGGTGCGCCA                                                                                                                         >  SRR19973065.2846014/1‑150 (MQ=60)
                            AATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCA                                                                                                                         >  SRR19973065.2845988/1‑150 (MQ=60)
                            AATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCA                                                                                                                         >  SRR19973065.1842973/1‑150 (MQ=60)
                            AATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCA                                                                                                                         >  SRR19973065.2844508/1‑150 (MQ=60)
                            AATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGGGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCA                                                                                                                         >  SRR19973065.2844488/1‑150 (MQ=60)
                            AATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGGGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCA                                                                                                                         >  SRR19973065.2844489/1‑150 (MQ=60)
                            AATTTTGATACCCGCATAGGCGTGCGGGCGCTAATTACCGTTACCCCGGGCGGAAAACAGGTGCCGTTTGTCTCACTGGGACCGGAAAACAATAACTGAATAAACAGTATTGTTGGTGATTAAGGGCAAGTTTAATTAATTGGTGCCCCA                                                                                                                         >  SRR19973065.2997599/1‑150 (MQ=55)
                              TTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATT                                                                                                                       <  SRR19973065.183622/150‑1 (MQ=60)
                              TTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATT                                                                                                                       <  SRR19973065.1967991/150‑1 (MQ=60)
                                 TGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATAACTGGTACGGGAAAACAGTACCGGGATAACCCGTATGGTGGGTGATTTCGGGCAAGTTTATTTAAGTGGTGCGCCATTGGC                                                                                                                    >  SRR19973065.1501703/1‑150 (MQ=60)
                                  GATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCT                                                                                                                   >  SRR19973065.2843908/1‑150 (MQ=60)
                                  GATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCT                                                                                                                   <  SRR19973065.429442/150‑1 (MQ=60)
                                  GATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCT                                                                                                                   <  SRR19973065.432126/150‑1 (MQ=60)
                                  GATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCT                                                                                                                   <  SRR19973065.428639/150‑1 (MQ=60)
                                  GATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCT                                                                                                                   <  SRR19973065.490293/150‑1 (MQ=60)
                                   ATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTG                                                                                                                  >  SRR19973065.464675/1‑150 (MQ=60)
                                   ATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCAGTGTCTG                                                                                                                  >  SRR19973065.1921817/1‑150 (MQ=60)
                                    TACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGG                                                                                                                 <  SRR19973065.1814620/150‑1 (MQ=60)
                                     ACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGT                                                                                                                >  SRR19973065.183545/1‑150 (MQ=60)
                                         TCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGTTGCGCCATTGTCTGGTGAAT                                                                                                            <  SRR19973065.1830850/150‑1 (MQ=60)
                                            TAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACAGGTAAGGGAAAACAGTACCGGAATAACCAGTATGGTTGGTGATTACGGGCAACTTTATTTAAATGGTGCGCCATTGTCTGGTGAAataa                                                                                                         >  SRR19973065.599840/1‑146 (MQ=60)
                                                 GTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACACTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCCTTGTCTGGTGAATTACTGGTT                                                                                                    >  SRR19973065.2715072/1‑150 (MQ=60)
                                                     GGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTTAATTACTGGTTCAGT                                                                                                <  SRR19973065.3403775/150‑1 (MQ=60)
                                                     GGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGT                                                                                                <  SRR19973065.1225896/150‑1 (MQ=60)
                                                      GGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTTGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGTTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTG                                                                                               <  SRR19973065.1668934/150‑1 (MQ=60)
                                                      GGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTG                                                                                               <  SRR19973065.3414639/150‑1 (MQ=60)
                                                          CTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGA                                                                                           >  SRR19973065.3292155/1‑150 (MQ=60)
                                                           TCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAG                                                                                          <  SRR19973065.1470409/150‑1 (MQ=60)
                                                             ATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCCGTCTGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCGGTGGGGAGAC                                                                                        >  SRR19973065.187662/1‑150 (MQ=60)
                                                             ATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTCTTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGAC                                                                                        >  SRR19973065.2660763/1‑150 (MQ=60)
                                                                   GTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCG                                                                                  >  SRR19973065.2785105/1‑150 (MQ=60)
                                                                   GTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCG                                                                                  >  SRR19973065.2785103/1‑150 (MQ=60)
                                                                   GTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCG                                                                                  <  SRR19973065.2995557/150‑1 (MQ=60)
                                                                    TTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGA                                                                                 <  SRR19973065.260277/150‑1 (MQ=60)
                                                                         CAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCA                                                                            >  SRR19973065.1352605/1‑150 (MQ=60)
                                                                              CGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAAACAGTATGGTGGGTGATTACGGGCAAGTTTATTTAATTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTG                                                                       >  SRR19973065.3354082/1‑150 (MQ=60)
                                                                                 AAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCAT                                                                    <  SRR19973065.2663081/150‑1 (MQ=60)
                                                                                   AACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTG                                                                  >  SRR19973065.2373930/1‑150 (MQ=60)
                                                                                       GGTGCCGTTTGGATCACTGGTAAGGGAAAACAGTACCGGAAAAAACAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTAATGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCA                                                              >  SRR19973065.2698944/1‑150 (MQ=60)
                                                                                          GCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTA                                                           >  SRR19973065.166738/1‑150 (MQ=60)
                                                                                          GCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTA                                                           >  SRR19973065.166743/1‑150 (MQ=60)
                                                                                           CCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTAT                                                          >  SRR19973065.434958/1‑150 (MQ=60)
                                                                                             GTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGT                                                        >  SRR19973065.2676197/1‑150 (MQ=60)
                                                                                                   ATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCC                                                  <  SRR19973065.1761645/150‑1 (MQ=60)
                                                                                                   ATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCC                                                  <  SRR19973065.1761750/150‑1 (MQ=60)
                                                                                                      ACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAA                                               <  SRR19973065.2595875/150‑1 (MQ=60)
                                                                                                      ACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCAATATGTATTGCCGAA                                               <  SRR19973065.2595437/150‑1 (MQ=60)
                                                                                                        TGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATGTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGC                                             >  SRR19973065.2719284/1‑150 (MQ=60)
                                                                                                         GGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCA                                            >  SRR19973065.289352/1‑150 (MQ=60)
                                                                                                             CGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGC                                        >  SRR19973065.937784/1‑150 (MQ=60)
                                                                                                             CGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGC                                        >  SRR19973065.1727726/1‑150 (MQ=60)
                                                                                                                  AAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACA                                   >  SRR19973065.1008198/1‑150 (MQ=60)
                                                                                                                  AAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACA                                   >  SRR19973065.1008055/1‑150 (MQ=60)
                                                                                                                  AAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACA                                   <  SRR19973065.1906408/150‑1 (MQ=60)
                                                                                                                  AAACAGTACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACA                                   <  SRR19973065.887842/150‑1 (MQ=60)
                                                                                                                  AAACAGGACCGGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACA                                   <  SRR19973065.3239563/150‑1 (MQ=60)
                                                                                                                         ACCGGAATAACCAGTATGGTGGGTGATTACGGTCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCC                            <  SRR19973065.1145090/150‑1 (MQ=60)
                                                                                                                            GGAATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGATGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTC                         >  SRR19973065.419425/1‑150 (MQ=60)
                                                                                                                              AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAC                       >  SRR19973065.1542881/1‑150 (MQ=60)
                                                                                                                              AATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAC                       >  SRR19973065.2641089/1‑150 (MQ=60)
                                                                                                                               ATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACT                      >  SRR19973065.1248114/1‑150 (MQ=60)
                                                                                                                               ATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACT                      >  SRR19973065.2632514/1‑150 (MQ=60)
                                                                                                                               ATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACT                      <  SRR19973065.3134294/150‑1 (MQ=60)
                                                                                                                               ATAACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATAGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAATCTTACAGCAAGCCGTCACT                      >  SRR19973065.3005810/1‑150 (MQ=60)
                                                                                                                                 AACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGT                    >  SRR19973065.383455/1‑150 (MQ=60)
                                                                                                                                 AACCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGT                    >  SRR19973065.383429/1‑150 (MQ=60)
                                                                                                                                   CCAGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTA                  <  SRR19973065.975008/150‑1 (MQ=60)
                                                                                                                                     AGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATT                >  SRR19973065.2338655/1‑150 (MQ=60)
                                                                                                                                     AGTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATT                >  SRR19973065.3514382/1‑150 (MQ=60)
                                                                                                                                      GTATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTT               >  SRR19973065.2536305/1‑150 (MQ=60)
                                                                                                                                        ATGGTGGGTGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCG             >  SRR19973065.1523330/1‑150 (MQ=60)
                                                                                                                                                TGATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTG     <  SRR19973065.2939382/150‑1 (MQ=60)
                                                                                                                                                 GATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGC    >  SRR19973065.2519042/1‑150 (MQ=60)
                                                                                                                                                  ATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCA   >  SRR19973065.2281347/1‑150 (MQ=60)
                                                                                                                                                  ATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCA   >  SRR19973065.2281499/1‑150 (MQ=60)
                                                                                                                                                  ATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCA   <  SRR19973065.2510438/150‑1 (MQ=60)
                                                                                                                                                  ATTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCA   <  SRR19973065.739024/150‑1 (MQ=60)
                                                                                                                                                   TTACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCAC  <  SRR19973065.1584927/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
GCCGACGCAAGGCGCGTTGGTTCGTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCACTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTCTGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCAC  >  CP009273/557444‑557740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑