Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,784 G→T 58.0% intergenic (+28/‑8) sfmD → / → sfmH putative outer membrane export usher protein/FimA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,7840GT58.0% 20.6 / inf 236intergenic (+28/‑8)sfmD/sfmHputative outer membrane export usher protein/FimA homolog
Reads supporting (aligned to +/- strand):  ref base G (64/35);  new base T (62/75);  total (126/110)
Fisher's exact test for biased strand distribution p-value = 3.67e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

Only 100 of 236 total aligned reads displayed.

CTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTTGTT  >  CP009273/557637‑557932
                                                                                                                                                   |                                                                                                                                                    
  ggTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATatta                                                                                                                                                  <  1:1406620/150‑1 (MQ=255)
     tCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATattatta                                                                                                                                               >  2:3825079/1‑150 (MQ=255)
     tCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGAAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGattatta                                                                                                                                               <  1:269309/150‑1 (MQ=255)
         tGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTgg                                                                                                                                           <  1:948049/150‑1 (MQ=255)
          ggggAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACCGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGc                                                                                                                                          >  1:2882768/1‑150 (MQ=255)
           gggAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCg                                                                                                                                         >  2:563528/1‑150 (MQ=255)
              agaCGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATg                                                                                                                                      >  2:3612706/1‑150 (MQ=255)
              agaCGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATg                                                                                                                                      >  2:4550950/1‑150 (MQ=255)
               gaCGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATgg                                                                                                                                     <  2:751019/150‑1 (MQ=255)
                       gAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTtg                                                                                                                             >  1:2661037/1‑150 (MQ=255)
                        aaCTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTtgt                                                                                                                            >  2:1599256/1‑150 (MQ=255)
                          cTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTgtgt                                                                                                                          <  2:3094728/150‑1 (MQ=255)
                           tCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTc                                                                                                                         <  1:4085016/150‑1 (MQ=255)
                           tCACGCTGCATTGCGCACTATGTAGTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTc                                                                                                                         <  1:714790/150‑1 (MQ=255)
                             aCGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTg                                                                                                                       <  1:1041133/150‑1 (MQ=255)
                             aCGCTGCATTGCGCACTATGTAGTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATACAGGAAATTATCAATAAGATAATCTGCAGAGTATTATTGGCGATGGCATGTTTGTGTCTg                                                                                                                       <  2:4590171/150‑1 (MQ=255)
                                cTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCa                                                                                                                    <  1:2203592/150‑1 (MQ=255)
                                 tGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCaa                                                                                                                   <  1:1325803/150‑1 (MQ=255)
                                   cATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAAc                                                                                                                 >  2:3030263/1‑150 (MQ=255)
                                    aTTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACa                                                                                                                <  2:1627758/150‑1 (MQ=255)
                                    aTTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACa                                                                                                                >  2:3355744/1‑150 (MQ=255)
                                        cgcACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATc                                                                                                            >  2:3916560/1‑150 (MQ=255)
                                        cgcACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCAGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGATCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATc                                                                                                            <  1:3100898/150‑1 (MQ=255)
                                             tATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTggg                                                                                                       >  2:3841584/1‑150 (MQ=255)
                                               tGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCt                                                                                                     >  1:49802/1‑150 (MQ=255)
                                                gTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTa                                                                                                    >  1:996205/1‑150 (MQ=255)
                                                      ccGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGttt                                                                                              >  2:2041656/1‑150 (MQ=255)
                                                       cGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtg                                                                                             >  1:2470942/1‑150 (MQ=255)
                                                       cGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtg                                                                                             >  1:1891927/1‑150 (MQ=255)
                                                       cGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtg                                                                                             >  2:1846026/1‑150 (MQ=255)
                                                       cGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtg                                                                                             >  2:3243999/1‑150 (MQ=255)
                                                        gAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtgt                                                                                            <  2:4434839/150‑1 (MQ=255)
                                                        gAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtgt                                                                                            >  1:3554530/1‑150 (MQ=255)
                                                         aaGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtgtg                                                                                           >  2:926135/1‑150 (MQ=255)
                                                         aaGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtgtg                                                                                           <  1:970463/150‑1 (MQ=255)
                                                           gCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCa                                                                                         <  2:241085/150‑1 (MQ=255)
                                                              aaGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAAt                                                                                      >  2:2468414/1‑150 (MQ=255)
                                                               aGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATa                                                                                     <  1:2365560/150‑1 (MQ=255)
                                                                   tACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTAc                                                                                 >  1:4170920/1‑150 (MQ=255)
                                                                    aCAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACAACCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACAGTTTGTGCAAATAGTACt                                                                                >  1:3171989/1‑150 (MQ=255)
                                                                      aGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTgg                                                                              >  2:12648/1‑150 (MQ=255)
                                                                       gCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGc                                                                             <  1:2802073/150‑1 (MQ=255)
                                                                             cGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCa                                                                       <  2:1269431/150‑1 (MQ=255)
                                                                             cGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCa                                                                       >  1:4296520/1‑150 (MQ=255)
                                                                               tCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCaga                                                                     >  1:4341412/1‑150 (MQ=255)
                                                                                  cTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCagaaga                                                                  >  2:587431/1‑150 (MQ=255)
                                                                                  cTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCagaaga                                                                  >  2:2280889/1‑150 (MQ=255)
                                                                                   tGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGAt                                                                 <  2:1229282/150‑1 (MQ=255)
                                                                                     ttATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGa                                                               >  2:3917311/1‑150 (MQ=255)
                                                                                       aTTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAac                                                             >  1:1095047/1‑150 (MQ=255)
                                                                                       aTTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAac                                                             >  1:1999180/1‑150 (MQ=255)
                                                                                        tttCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAaca                                                            <  1:1502897/150‑1 (MQ=255)
                                                                                         ttCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAacac                                                           >  1:3070831/1‑150 (MQ=255)
                                                                                            ggCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTAt                                                        <  1:2550675/150‑1 (MQ=255)
                                                                                            ggCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTAt                                                        >  1:1384306/1‑150 (MQ=255)
                                                                                             gCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATg                                                       <  2:1483519/150‑1 (MQ=255)
                                                                                                  ttGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAtctc                                                  <  1:3999524/150‑1 (MQ=255)
                                                                                                   tGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAtctct                                                 <  2:4621155/150‑1 (MQ=255)
                                                                                                     cacacaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCa                                               >  1:134618/1‑150 (MQ=255)
                                                                                                      acacaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCaa                                              <  2:2663593/150‑1 (MQ=255)
                                                                                                       cacaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCaaa                                             <  1:168104/150‑1 (MQ=255)
                                                                                                        acaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAat                                            <  1:2758183/150‑1 (MQ=255)
                                                                                                        acaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAat                                            <  1:1976469/150‑1 (MQ=255)
                                                                                                          aTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAatat                                          <  2:3150355/150‑1 (MQ=255)
                                                                                                          aTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAatat                                          >  1:3813733/1‑150 (MQ=255)
                                                                                                          aTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAatat                                          >  1:3348319/1‑150 (MQ=255)
                                                                                                           tCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATc                                         >  1:1012084/1‑150 (MQ=255)
                                                                                                            ccTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCt                                        >  1:2912009/1‑150 (MQ=255)
                                                                                                            ccTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCt                                        >  2:3520804/1‑150 (MQ=255)
                                                                                                            ccTGGCTCATAAACGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATTGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGAGTAGCAGAAGATGAACACTATGATCTCTCAAATATCt                                        <  1:4627123/150‑1 (MQ=255)
                                                                                                               ggCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTa                                     <  1:2843575/150‑1 (MQ=255)
                                                                                                                gCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTaa                                    <  1:1042089/150‑1 (MQ=255)
                                                                                                                 cTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAAt                                   >  2:2760902/1‑150 (MQ=255)
                                                                                                                 cTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAAt                                   >  2:2799415/1‑150 (MQ=255)
                                                                                                                  tCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATa                                  >  1:3861025/1‑150 (MQ=255)
                                                                                                                  tCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTGTGTGTCTGGCAAACATAACCTGGGCTACTGTTTGTGCAAATAGTACTGGCTTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATa                                  >  1:4590753/1‑150 (MQ=255)
                                                                                                                     tAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCa                               >  1:2062356/1‑150 (MQ=255)
                                                                                                                      aaaGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCAc                              >  1:650325/1‑150 (MQ=255)
                                                                                                                       aaGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCAcc                             <  1:1116837/150‑1 (MQ=255)
                                                                                                                         ggAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCaa                           >  1:1846729/1‑150 (MQ=255)
                                                                                                                         ggAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCaa                           <  1:3180564/150‑1 (MQ=255)
                                                                                                                         ggAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCaa                           >  2:4366094/1‑150 (MQ=255)
                                                                                                                         ggAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCaa                           >  2:498436/1‑150 (MQ=255)
                                                                                                                             aTTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAc                       <  1:1072046/150‑1 (MQ=255)
                                                                                                                              ttATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAcc                      <  2:4049052/150‑1 (MQ=255)
                                                                                                                               tATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAcca                     <  2:960690/150‑1 (MQ=255)
                                                                                                                                aTCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccag                    <  2:3421275/150‑1 (MQ=255)
                                                                                                                                aTCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccag                    <  1:563528/150‑1 (MQ=255)
                                                                                                                                 tCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagc                   >  2:665408/1‑150 (MQ=255)
                                                                                                                                   aaTAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagcca                 <  1:2041679/150‑1 (MQ=255)
                                                                                                                                    aTAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagccag                >  1:3332453/1‑150 (MQ=255)
                                                                                                                                    aTAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagccag                >  1:791214/1‑150 (MQ=255)
                                                                                                                                    aTAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagccag                >  2:83908/1‑150 (MQ=255)
                                                                                                                                      aaGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAggg              >  1:2174577/1‑150 (MQ=255)
                                                                                                                                       aGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGc             >  2:2810207/1‑150 (MQ=255)
                                                                                                                                       aGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGc             <  2:2057232/150‑1 (MQ=255)
                                                                                                                                        gATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCa            <  1:4331779/150‑1 (MQ=255)
                                                                                                                                        gATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCa            <  1:63473/150‑1 (MQ=255)
                                                                                                                                           aaTCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGAt         >  2:3256454/1‑150 (MQ=255)
                                                                                                                                                  aGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTgttgtt  >  2:1708412/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTTGTT  >  CP009273/557637‑557932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTTGT  >  CP009273/557635‑557931
                                                                                                                                                     |                                                                                                                                                   
TACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAT                                                                                                                                                     <  SRR19973072.950516/150‑1 (MQ=60)
    GGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTA                                                                                                                                                 <  SRR19973072.1409475/150‑1 (MQ=60)
    GGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTA                                                                                                                                                 <  SRR19973072.1409682/150‑1 (MQ=60)
      TTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATT                                                                                                                                               <  SRR19973072.1454133/150‑1 (MQ=60)
       TCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGAAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTA                                                                                                                                              <  SRR19973072.269944/150‑1 (MQ=60)
          GTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTG                                                                                                                                           >  SRR19973072.4086207/1‑150 (MQ=60)
           TGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGG                                                                                                                                          <  SRR19973072.950122/150‑1 (MQ=60)
           TGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGG                                                                                                                                          <  SRR19973072.950247/150‑1 (MQ=60)
           TGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGG                                                                                                                                          <  SRR19973072.287458/150‑1 (MQ=60)
           TGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGG                                                                                                                                          <  SRR19973072.649709/150‑1 (MQ=60)
           TGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGG                                                                                                                                          <  SRR19973072.2813020/150‑1 (MQ=60)
            GGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACCGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGC                                                                                                                                         >  SRR19973072.2888948/1‑150 (MQ=60)
            GGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACCGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGC                                                                                                                                         >  SRR19973072.2888933/1‑150 (MQ=60)
             GGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCG                                                                                                                                        >  SRR19973072.2691697/1‑150 (MQ=60)
               GAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGAT                                                                                                                                      <  SRR19973072.2060420/150‑1 (MQ=60)
                 GACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCTAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGTAAATTATCAATAAGATTATCTGCATATTATTATTGGCGATGG                                                                                                                                    <  SRR19973072.884522/150‑1 (MQ=60)
                 GACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGG                                                                                                                                    <  SRR19973072.2763676/150‑1 (MQ=60)
                 GACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGG                                                                                                                                    <  SRR19973072.2763782/150‑1 (MQ=60)
                         GAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTG                                                                                                                            >  SRR19973072.2666734/1‑150 (MQ=60)
                         GAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTG                                                                                                                            <  SRR19973072.322287/150‑1 (MQ=60)
                          AACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGT                                                                                                                           >  SRR19973072.2988139/1‑150 (MQ=60)
                           ACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTG                                                                                                                          >  SRR19973072.3619488/1‑150 (MQ=60)
                             TCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTC                                                                                                                        <  SRR19973072.4093590/150‑1 (MQ=60)
                             TCACGCTGCATTGCGCACTATGTAGTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTC                                                                                                                        <  SRR19973072.716342/150‑1 (MQ=60)
                              CACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCT                                                                                                                       >  SRR19973072.99194/1‑150 (MQ=60)
                               ACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTG                                                                                                                      <  SRR19973072.1043379/150‑1 (MQ=60)
                                  CTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCA                                                                                                                   <  SRR19973072.2208385/150‑1 (MQ=60)
                                  CTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCA                                                                                                                   <  SRR19973072.2208060/150‑1 (MQ=60)
                                   TGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAA                                                                                                                  >  SRR19973072.1368188/1‑150 (MQ=60)
                                   TGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAA                                                                                                                  <  SRR19973072.1328704/150‑1 (MQ=60)
                                   TGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAA                                                                                                                  <  SRR19973072.1328702/150‑1 (MQ=60)
                                    GCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAA                                                                                                                 >  SRR19973072.2061709/1‑150 (MQ=60)
                                          CGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATC                                                                                                           >  SRR19973072.1390128/1‑150 (MQ=60)
                                          CGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCAGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGATCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATC                                                                                                           <  SRR19973072.3107434/150‑1 (MQ=60)
                                          CGCACTATGTATAGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATC                                                                                                           <  SRR19973072.3105880/150‑1 (MQ=60)
                                            CACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCT                                                                                                         >  SRR19973072.4186782/1‑150 (MQ=60)
                                             ACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTG                                                                                                        <  SRR19973072.1567565/150‑1 (MQ=60)
                                              CTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGG                                                                                                       <  SRR19973072.611931/150‑1 (MQ=60)
                                                 TGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCT                                                                                                    >  SRR19973072.49934/1‑150 (MQ=60)
                                                  GTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTA                                                                                                   >  SRR19973072.998388/1‑150 (MQ=60)
                                                      TGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGT                                                                                               <  SRR19973072.2442953/150‑1 (MQ=60)
                                                       GCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTT                                                                                              <  SRR19973072.2626105/150‑1 (MQ=60)
                                                         CGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTG                                                                                            >  SRR19973072.1895977/1‑150 (MQ=60)
                                                         CGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTG                                                                                            >  SRR19973072.2476309/1‑150 (MQ=60)
                                                          GAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGT                                                                                           >  SRR19973072.1695634/1‑150 (MQ=60)
                                                          GAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGT                                                                                           >  SRR19973072.3562001/1‑150 (MQ=60)
                                                           AAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTG                                                                                          <  SRR19973072.972587/150‑1 (MQ=60)
                                                             GCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCA                                                                                        <  SRR19973072.2545026/150‑1 (MQ=60)
                                                                 AGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATA                                                                                    <  SRR19973072.2370220/150‑1 (MQ=60)
                                                                 AGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATA                                                                                    <  SRR19973072.2370656/150‑1 (MQ=60)
                                                                 AGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATA                                                                                    >  SRR19973072.3958935/1‑150 (MQ=60)
                                                                    TTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTA                                                                                 >  SRR19973072.1058686/1‑150 (MQ=60)
                                                                    TTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTA                                                                                 >  SRR19973072.1058543/1‑150 (MQ=60)
                                                                     TACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTAC                                                                                >  SRR19973072.4179654/1‑150 (MQ=60)
                                                                     TACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTAC                                                                                >  SRR19973072.4179651/1‑150 (MQ=60)
                                                                      ACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACT                                                                               >  SRR19973072.3178893/1‑150 (MQ=60)
                                                                      ACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACAACCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACAGTTTGTGCAAATAGTACT                                                                               >  SRR19973072.3178681/1‑150 (MQ=60)
                                                                         GCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGC                                                                            <  SRR19973072.2808066/150‑1 (MQ=60)
                                                                         GCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGC                                                                            <  SRR19973072.2808074/150‑1 (MQ=60)
                                                                               CGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCA                                                                      <  SRR19973072.1091930/150‑1 (MQ=60)
                                                                               CGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCA                                                                      >  SRR19973072.4305475/1‑150 (MQ=60)
                                                                                 TCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGA                                                                    >  SRR19973072.4350500/1‑150 (MQ=60)
                                                                                   ACTGTTATGTCGGCAGCTTGCACACATCCTGGCTCATAAAGGCAATTATCAATAAGATAATCTGCAGATTATTATGGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAG                                                                  <  SRR19973072.1891177/150‑1 (MQ=60)
                                                                                      GTTATTTCGGCAGTTTGCACACATCCTGGCTCATAATGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATG                                                               <  SRR19973072.4307043/150‑1 (MQ=60)
                                                                                         ATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAAC                                                            >  SRR19973072.1097363/1‑150 (MQ=60)
                                                                                         ATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAAC                                                            >  SRR19973072.2003486/1‑150 (MQ=60)
                                                                                         ATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAAC                                                            >  SRR19973072.1097872/1‑150 (MQ=60)
                                                                                          TTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACA                                                           <  SRR19973072.1506098/150‑1 (MQ=60)
                                                                                           TTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACAC                                                          >  SRR19973072.3077317/1‑150 (MQ=60)
                                                                                           TTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACAC                                                          >  SRR19973072.3077323/1‑150 (MQ=60)
                                                                                            TCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACT                                                         >  SRR19973072.334931/1‑150 (MQ=60)
                                                                                              GGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTAT                                                       <  SRR19973072.2556211/150‑1 (MQ=60)
                                                                                              GGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTAT                                                       >  SRR19973072.535587/1‑150 (MQ=60)
                                                                                              GGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTAT                                                       >  SRR19973072.1387324/1‑150 (MQ=60)
                                                                                                  GTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATC                                                   >  SRR19973072.1954850/1‑150 (MQ=60)
                                                                                                   TTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCT                                                  <  SRR19973072.2171653/150‑1 (MQ=60)
                                                                                                    TTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTC                                                 <  SRR19973072.4007900/150‑1 (MQ=60)
                                                                                                       CACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCA                                              >  SRR19973072.134960/1‑150 (MQ=60)
                                                                                                         CACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAA                                            <  SRR19973072.168534/150‑1 (MQ=60)
                                                                                                         CACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAA                                            >  SRR19973072.779207/1‑150 (MQ=60)
                                                                                                         CACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAA                                            <  SRR19973072.3833075/150‑1 (MQ=60)
                                                                                                          ACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAAT                                           <  SRR19973072.2764082/150‑1 (MQ=60)
                                                                                                          ACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAAT                                           <  SRR19973072.1980702/150‑1 (MQ=60)
                                                                                                            ATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATAT                                         >  SRR19973072.3355416/1‑150 (MQ=60)
                                                                                                            ATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATAT                                         >  SRR19973072.3356382/1‑150 (MQ=60)
                                                                                                            ATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATAT                                         >  SRR19973072.3821709/1‑150 (MQ=60)
                                                                                                             TCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATC                                        >  SRR19973072.1014466/1‑150 (MQ=60)
                                                                                                             TCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATC                                        >  SRR19973072.1014289/1‑150 (MQ=60)
                                                                                                             TCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATC                                        >  SRR19973072.1995009/1‑150 (MQ=60)
                                                                                                              CCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCT                                       >  SRR19973072.2918062/1‑150 (MQ=60)
                                                                                                              CCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCT                                       >  SRR19973072.2918248/1‑150 (MQ=60)
                                                                                                              CCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTAATGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCT                                       >  SRR19973072.4460307/1‑150 (MQ=60)
                                                                                                              CCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCT                                       <  SRR19973072.4636867/150‑1 (MQ=60)
                                                                                                              CCTGGCTCATAAACGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATTGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGAGTAGCAGAAGATGAACACTATGATCTCTCAAATATCT                                       <  SRR19973072.4636811/150‑1 (MQ=60)
                                                                                                                TGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTT                                     >  SRR19973072.2255716/1‑150 (MQ=60)
                                                                                                                 GGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTA                                    <  SRR19973072.2849658/150‑1 (MQ=60)
                                                                                                                 GGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTA                                    <  SRR19973072.1849724/150‑1 (MQ=60)
                                                                                                                  GCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAA                                   <  SRR19973072.1044337/150‑1 (MQ=60)
                                                                                                                   CTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAAT                                  >  SRR19973072.3234956/1‑150 (MQ=60)
                                                                                                                    TCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATA                                 >  SRR19973072.3869061/1‑150 (MQ=60)
                                                                                                                    TCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATA                                 >  SRR19973072.4600393/1‑150 (MQ=60)
                                                                                                                    TCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTGTGTGTCTGGCAAACATAACCTGGGCTACTGTTTGTGCAAATAGTACTGGCTTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATA                                 >  SRR19973072.4600392/1‑150 (MQ=60)
                                                                                                                      ATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGC                               >  SRR19973072.691365/1‑150 (MQ=60)
                                                                                                                       TAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCA                              >  SRR19973072.2066844/1‑150 (MQ=60)
                                                                                                                        AAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCAC                             >  SRR19973072.651797/1‑150 (MQ=60)
                                                                                                                         AAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACC                            <  SRR19973072.1119192/150‑1 (MQ=60)
                                                                                                                          AGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCA                           <  SRR19973072.3925484/150‑1 (MQ=60)
                                                                                                                           GGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAA                          <  SRR19973072.3187266/150‑1 (MQ=60)
                                                                                                                           GGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAA                          >  SRR19973072.1850729/1‑150 (MQ=60)
                                                                                                                             AAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATA                        >  SRR19973072.4266729/1‑150 (MQ=60)
                                                                                                                               ATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAC                      <  SRR19973072.1074332/150‑1 (MQ=60)
                                                                                                                               ATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAC                      <  SRR19973072.4471404/150‑1 (MQ=60)
                                                                                                                                  ATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAG                   <  SRR19973072.1934705/150‑1 (MQ=60)
                                                                                                                                  ATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAG                   <  SRR19973072.564848/150‑1 (MQ=60)
                                                                                                                                  ATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAG                   <  SRR19973072.930932/150‑1 (MQ=60)
                                                                                                                                  ATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAG                   <  SRR19973072.564671/150‑1 (MQ=60)
                                                                                                                                   TCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGC                  >  SRR19973072.3761056/1‑150 (MQ=60)
                                                                                                                                    CAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCC                 <  SRR19973072.131821/150‑1 (MQ=60)
                                                                                                                                     AATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCA                <  SRR19973072.1044328/150‑1 (MQ=60)
                                                                                                                                     AATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCA                <  SRR19973072.2046115/150‑1 (MQ=60)
                                                                                                                                     AATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCA                <  SRR19973072.2046092/150‑1 (MQ=60)
                                                                                                                                      ATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAG               >  SRR19973072.3339524/1‑150 (MQ=60)
                                                                                                                                      ATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAG               >  SRR19973072.792902/1‑150 (MQ=60)
                                                                                                                                      ATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAG               >  SRR19973072.3120184/1‑150 (MQ=60)
                                                                                                                                        AAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGG             >  SRR19973072.2179315/1‑150 (MQ=60)
                                                                                                                                          GATTATCTGAATATTATTATTGGCGTTGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCA           <  SRR19973072.63724/150‑1 (MQ=60)
                                                                                                                                          GATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCA           <  SRR19973072.63641/150‑1 (MQ=60)
                                                                                                                                          GATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCA           <  SRR19973072.4340865/150‑1 (MQ=60)
                                                                                                                                          GATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCA           <  SRR19973072.4340842/150‑1 (MQ=60)
                                                                                                                                            TAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGA         <  SRR19973072.2565353/150‑1 (MQ=60)
                                                                                                                                              ATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATT       >  SRR19973072.1200923/1‑150 (MQ=60)
                                                                                                                                                   CATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTTGT  >  SRR19973072.4127613/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTTGT  >  CP009273/557635‑557931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑