Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,784 G→T 17.8% intergenic (+28/‑8) sfmD → / → sfmH putative outer membrane export usher protein/FimA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,7840GT17.8% 756.3 / 102.3 250intergenic (+28/‑8)sfmD/sfmHputative outer membrane export usher protein/FimA homolog
Reads supporting (aligned to +/- strand):  ref base G (116/89);  new base T (24/21);  total (140/110)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

ACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTTGTTT  >  CP009273/557636‑557933
                                                                                                                                                    |                                                                                                                                                     
aCTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGa                                                                                                                                                      >  2:1115747/1‑150 (MQ=255)
 cTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGat                                                                                                                                                     <  2:445413/150‑1 (MQ=255)
  tGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGatt                                                                                                                                                    <  2:1895221/150‑1 (MQ=255)
  tGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATATCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGatt                                                                                                                                                    >  2:1734043/1‑150 (MQ=255)
   ggTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATatta                                                                                                                                                   >  1:4480962/1‑150 (MQ=255)
     ttCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTCTGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATattatt                                                                                                                                                 <  2:3061295/150‑1 (MQ=255)
     ttCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGattatt                                                                                                                                                 <  1:952161/150‑1 (MQ=255)
       cAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGattattat                                                                                                                                               <  2:346988/150‑1 (MQ=255)
        aGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGattattatt                                                                                                                                              >  1:4593746/1‑150 (MQ=255)
        aGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGattattatt                                                                                                                                              <  1:2579771/150‑1 (MQ=255)
        aGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGattattatt                                                                                                                                              >  1:478731/1‑150 (MQ=255)
          tGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTgg                                                                                                                                            >  2:3709773/1‑150 (MQ=255)
           ggggAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGc                                                                                                                                           <  2:376235/150‑1 (MQ=255)
           ggggAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGc                                                                                                                                           >  1:627546/1‑150 (MQ=255)
                   ggCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCAt                                                                                                                                   >  1:1011072/1‑150 (MQ=255)
                     cgcgAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGt                                                                                                                                 >  1:1529205/1‑150 (MQ=255)
                      gcgAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATACTCTGCAGATTATTATTGGCGATGGCATGtt                                                                                                                                >  1:19317/1‑150 (MQ=255)
                        gAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTtg                                                                                                                              >  1:2650830/1‑150 (MQ=255)
                             cACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCt                                                                                                                         >  1:3900881/1‑150 (MQ=255)
                               cGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTgg                                                                                                                       <  1:460197/150‑1 (MQ=255)
                                gCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGc                                                                                                                      >  2:786057/1‑150 (MQ=255)
                                 cTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCa                                                                                                                     >  1:3976904/1‑150 (MQ=255)
                                  tGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCaa                                                                                                                    <  1:1370672/150‑1 (MQ=255)
                                     aTTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACa                                                                                                                 <  1:853321/150‑1 (MQ=255)
                                        gcgcACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACatat                                                                                                              <  2:2831345/150‑1 (MQ=255)
                                        gcgcACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACatat                                                                                                              <  1:3610640/150‑1 (MQ=255)
                                         cgcACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATc                                                                                                             >  2:3159012/1‑150 (MQ=255)
                                           cACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCt                                                                                                           <  2:1139027/150‑1 (MQ=255)
                                            aCTATGTATTGCCGAAGCAAAGCTTACAGCAAGACGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCTGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTg                                                                                                          <  1:477068/150‑1 (MQ=255)
                                               aTGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGc                                                                                                       >  1:4030667/1‑150 (MQ=255)
                                                tGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCt                                                                                                      >  2:273812/1‑150 (MQ=255)
                                                tGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCt                                                                                                      >  2:2907700/1‑150 (MQ=255)
                                                 gTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTa                                                                                                     >  2:1644638/1‑150 (MQ=255)
                                                   aTTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCAGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACt                                                                                                   >  2:1431855/1‑150 (MQ=255)
                                                         gAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtgt                                                                                             >  2:427624/1‑150 (MQ=255)
                                                         gAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCAGAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtgt                                                                                             >  2:4171297/1‑150 (MQ=255)
                                                         gAAGCAAAGCTAACAGCAAGCCGTAACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGTCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGtgagt                                                                                             >  2:2278044/1‑146 (MQ=255)
                                                          aaGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTtgtg                                                                                            <  2:3276164/150‑1 (MQ=255)
                                                              aaaGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCaaa                                                                                        >  2:725028/1‑150 (MQ=255)
                                                              aaaGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCaaa                                                                                        >  2:3930653/1‑150 (MQ=255)
                                                               aaGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAAt                                                                                       <  1:1321871/150‑1 (MQ=255)
                                                                  cTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAACTTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGt                                                                                    <  2:1452601/150‑1 (MQ=255)
                                                                  cTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGt                                                                                    <  2:2600407/150‑1 (MQ=255)
                                                                    tACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTAc                                                                                  >  1:1526196/1‑150 (MQ=255)
                                                                    tACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTAc                                                                                  <  1:1728202/150‑1 (MQ=255)
                                                                     aCAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACt                                                                                 <  2:3298974/150‑1 (MQ=255)
                                                                        gCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGc                                                                              <  2:2650830/150‑1 (MQ=255)
                                                                        gCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATATTACTGGc                                                                              >  2:1900057/1‑150 (MQ=255)
                                                                           aGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTa                                                                           <  1:2121120/150‑1 (MQ=255)
                                                                              cGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCa                                                                        <  1:1605893/150‑1 (MQ=255)
                                                                                  aCTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCagaag                                                                    <  2:156623/150‑1 (MQ=255)
                                                                                   cTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGACATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATACTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCagaaga                                                                   >  2:166180/1‑150 (MQ=255)
                                                                                    tGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGAt                                                                  >  2:3372056/1‑150 (MQ=255)
                                                                                     gTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATg                                                                 >  1:3292280/1‑150 (MQ=255)
                                                                                     gTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATg                                                                 <  1:2527980/150‑1 (MQ=255)
                                                                                      ttATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATACTCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGa                                                                >  2:253615/1‑150 (MQ=255)
                                                                                      ttATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAATCATATCCTGGGCTACTGTGTGTGCAAATAGTACTGGCGTAGCAGAAGATGa                                                                >  2:1946030/1‑150 (MQ=255)
                                                                                      ttATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGa                                                                >  2:1918435/1‑150 (MQ=255)
                                                                                       tATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGaa                                                               >  2:3084675/1‑150 (MQ=255)
                                                                                        aTTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAac                                                              >  2:4378958/1‑150 (MQ=255)
                                                                                        aTTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAac                                                              >  2:1568184/1‑150 (MQ=255)
                                                                                        aTTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCGAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAac                                                              >  2:2333609/1‑150 (MQ=255)
                                                                                        aTTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAac                                                              <  1:2695171/150‑1 (MQ=255)
                                                                                          ttCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAacac                                                            <  2:2473779/150‑1 (MQ=255)
                                                                                           tCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACt                                                           >  1:1735066/1‑150 (MQ=255)
                                                                                            cGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTa                                                          <  2:1172138/150‑1 (MQ=255)
                                                                                              gCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATg                                                        <  2:4563801/150‑1 (MQ=255)
                                                                                                aGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAt                                                      >  1:1279613/1‑150 (MQ=255)
                                                                                                 gTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAtc                                                     >  1:2948045/1‑150 (MQ=255)
                                                                                                   ttGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAtctc                                                   >  1:3575203/1‑150 (MQ=255)
                                                                                                         acaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCAGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACGGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACCCTATGATCTCTCAAat                                             >  2:318090/1‑150 (MQ=255)
                                                                                                            tCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATc                                          <  2:3405249/150‑1 (MQ=255)
                                                                                                              cTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCtt                                        <  2:4249632/150‑1 (MQ=255)
                                                                                                                ggCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTa                                      >  1:239447/1‑150 (MQ=255)
                                                                                                                  cTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAAt                                    >  1:1502761/1‑150 (MQ=255)
                                                                                                                    cATAAAGTAAATTATCAATAAGATAATCTTCAGATTATAATAGGCGATGGCATGTATGTGTCTGGCAAACATATCCTTGGCTACAGTGTGTGCAAATAGTACTGGCGTAGCAGAAGATTAACACTATTATCTCTCAAATAACTATAATAg                                  >  1:698255/1‑150 (MQ=255)
                                                                                                                      tAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCa                                >  1:1118283/1‑150 (MQ=255)
                                                                                                                      tAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCa                                >  1:3390736/1‑150 (MQ=255)
                                                                                                                        aaGGAAATTATCAATAAGATACTCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCAcc                              >  2:3580980/1‑150 (MQ=255)
                                                                                                                          ggAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCaa                            <  1:222379/150‑1 (MQ=255)
                                                                                                                           gAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAAt                           <  1:3440067/150‑1 (MQ=255)
                                                                                                                           gAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAAt                           >  2:4617748/1‑150 (MQ=255)
                                                                                                                           gAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAAt                           <  1:3020368/150‑1 (MQ=255)
                                                                                                                             aaTTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATaa                         >  2:2753423/1‑150 (MQ=255)
                                                                                                                                tATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAcca                      <  1:2179767/150‑1 (MQ=255)
                                                                                                                                  tCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagc                    <  1:3974106/150‑1 (MQ=255)
                                                                                                                                   cAATAAGATAATCTTCAGAATATTATTGGCGATGGCAAGTTTGTGTCTGGCAAACATATACAGGGCTACTCATTGTGCAAAAAGAACTCCCGAAGCACAACATGAACACTCTCAACAATCAAATAAAATAAATAGCACAAATAAccagcc                   >  2:1033963/1‑150 (MQ=255)
                                                                                                                                    aaTAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagcca                  >  1:413889/1‑150 (MQ=255)
                                                                                                                                     aTAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagccag                 >  1:4031606/1‑150 (MQ=255)
                                                                                                                                     aTAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagccag                 >  1:2503902/1‑150 (MQ=255)
                                                                                                                                     aTAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagccag                 >  1:2503747/1‑150 (MQ=255)
                                                                                                                                      tAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAgg                <  2:2285607/150‑1 (MQ=255)
                                                                                                                                       aaGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAggg               >  2:17727/1‑150 (MQ=255)
                                                                                                                                       aaGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAggg               >  1:2863207/1‑150 (MQ=255)
                                                                                                                                        aGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGc              >  1:3850986/1‑150 (MQ=255)
                                                                                                                                          aTAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAg            <  2:2407413/150‑1 (MQ=255)
                                                                                                                                             aTCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGAtt         >  1:1834931/1‑150 (MQ=255)
                                                                                                                                               cTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGAttgt       <  1:3140490/150‑1 (MQ=255)
                                                                                                                                                  cAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATtgttgt    >  2:892552/1‑150 (MQ=255)
                                                                                                                                                   aGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTgttgtt   >  2:1326435/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTTGTTT  >  CP009273/557636‑557933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A