Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 557,784 G→T 50.6% intergenic (+28/‑8) sfmD → / → sfmH putative outer membrane export usher protein/FimA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273557,7840GT50.6% 89.4 / inf 247intergenic (+28/‑8)sfmD/sfmHputative outer membrane export usher protein/FimA homolog
Reads supporting (aligned to +/- strand):  ref base G (69/53);  new base T (78/47);  total (147/100)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-01

Only 100 of 250 total aligned reads displayed.

CTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTT  >  CP009273/557637‑557929
                                                                                                                                                   |                                                                                                                                                 
cTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGat                                                                                                                                                 <  1:3268586/150‑1 (MQ=255)
      cAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAtattattat                                                                                                                                           <  2:1111235/150‑1 (MQ=255)
       aGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGattattatt                                                                                                                                          <  1:4178642/150‑1 (MQ=255)
       aGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGattattatt                                                                                                                                          <  2:3995006/150‑1 (MQ=255)
        gTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTg                                                                                                                                         >  1:2023135/1‑150 (MQ=255)
         tGGGGAGACGGCGCGAACTCAAGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCTATAAGATAATCTGCATATTATTATTgg                                                                                                                                        <  2:2914652/150‑1 (MQ=255)
          ggggAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGc                                                                                                                                       <  1:2310418/150‑1 (MQ=255)
              agaCGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATg                                                                                                                                   >  2:21237/1‑150 (MQ=255)
               gaCGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATgg                                                                                                                                  >  1:1351685/1‑150 (MQ=255)
                aCGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGc                                                                                                                                 <  1:2770687/150‑1 (MQ=255)
                  ggCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCAt                                                                                                                               >  1:4101073/1‑150 (MQ=255)
                  ggCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCAt                                                                                                                               >  1:1881089/1‑150 (MQ=255)
                    agcgAACTCACGCTTCATGGCGAACTATGTATTGCCGAAGCAAAGCTTACAGCTAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATATAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGt                                                                                                                             <  2:76631/149‑1 (MQ=255)
                     gcgAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGtt                                                                                                                            <  2:2476924/150‑1 (MQ=255)
                     gcgAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGtt                                                                                                                            <  2:175940/150‑1 (MQ=255)
                         aCTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTAGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTtgtg                                                                                                                        >  2:1363503/1‑150 (MQ=255)
                          cTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTgtgt                                                                                                                       >  1:2498988/1‑150 (MQ=255)
                           tCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTc                                                                                                                      >  2:3898286/1‑150 (MQ=255)
                           tCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTc                                                                                                                      <  2:3797707/150‑1 (MQ=255)
                            cACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCt                                                                                                                     >  2:764893/1‑150 (MQ=255)
                                 tGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCaa                                                                                                                >  2:64441/1‑150 (MQ=255)
                                 tGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCaa                                                                                                                >  2:2368623/1‑150 (MQ=255)
                                   cATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAAc                                                                                                              >  1:3780762/1‑150 (MQ=255)
                                    aTTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACa                                                                                                             <  2:3079294/150‑1 (MQ=255)
                                    aTTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACa                                                                                                             <  2:797498/150‑1 (MQ=255)
                                      tGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACata                                                                                                           >  1:1074471/1‑150 (MQ=255)
                                         gcACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATcc                                                                                                        <  1:123350/150‑1 (MQ=255)
                                              aTGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGc                                                                                                   >  1:968576/1‑150 (MQ=255)
                                              aTGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGc                                                                                                   >  1:1250335/1‑150 (MQ=255)
                                              aTGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGc                                                                                                   >  1:1250243/1‑150 (MQ=255)
                                                           gCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCa                                                                                      <  2:4101073/150‑1 (MQ=255)
                                                             aaaGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCaaa                                                                                    >  1:4381844/1‑150 (MQ=255)
                                                                gCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAg                                                                                 <  2:1007510/150‑1 (MQ=255)
                                                                 cTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGt                                                                                >  1:2618257/1‑150 (MQ=255)
                                                                 cTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGt                                                                                <  2:2666867/150‑1 (MQ=255)
                                                                  ttACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTa                                                                               >  1:1879381/1‑150 (MQ=255)
                                                                   tACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTAc                                                                              <  1:4225273/150‑1 (MQ=255)
                                                                      aGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTgg                                                                           <  2:3615562/150‑1 (MQ=255)
                                                                         aaGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGt                                                                        >  1:3656944/1‑150 (MQ=255)
                                                                         aaGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGt                                                                        >  1:4017633/1‑150 (MQ=255)
                                                                          aGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTa                                                                       >  1:1056235/1‑150 (MQ=255)
                                                                              gTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCag                                                                   >  1:4347878/1‑150 (MQ=255)
                                                                              gTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCag                                                                   >  2:3870430/1‑150 (MQ=255)
                                                                               tCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCaga                                                                  <  2:1404126/150‑1 (MQ=255)
                                                                                  cTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCagaaga                                                               >  1:1628957/1‑150 (MQ=255)
                                                                                   tGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGAt                                                              >  1:3285618/1‑150 (MQ=255)
                                                                                      tATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGaa                                                           >  2:329217/1‑150 (MQ=255)
                                                                                         ttCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAacac                                                        <  1:934883/150‑1 (MQ=255)
                                                                                          tCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACt                                                       >  2:478654/1‑150 (MQ=255)
                                                                                           cGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTa                                                      >  2:3695931/1‑150 (MQ=255)
                                                                                           cGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTa                                                      <  2:3148402/150‑1 (MQ=255)
                                                                                           cGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTa                                                      <  2:1691627/150‑1 (MQ=255)
                                                                                             gCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATg                                                    <  1:338829/150‑1 (MQ=255)
                                                                                               aGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAt                                                  >  1:2127965/1‑150 (MQ=255)
                                                                                               aGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAt                                                  >  1:580179/1‑150 (MQ=255)
                                                                                                 tttGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAtct                                                <  1:3898286/150‑1 (MQ=255)
                                                                                                   tGCACACATCCTGGCTCATATTGTAAATTATCAATAAGATAATATGCAGATTATTATTGGCGATGGCATGTTTGTGTATGTCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGTACACTATGAtctct                                              <  2:2053122/150‑1 (MQ=255)
                                                                                                   tGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAtctct                                              >  2:36551/1‑150 (MQ=255)
                                                                                                   tGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAtctct                                              <  2:2610731/150‑1 (MQ=255)
                                                                                                    gCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCTTGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAtctctc                                             <  1:4436633/150‑1 (MQ=255)
                                                                                                    gCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAtctctc                                             <  1:859478/150‑1 (MQ=255)
                                                                                                       cacaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCaaa                                          <  1:764893/150‑1 (MQ=255)
                                                                                                        acaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAat                                         >  2:859533/1‑150 (MQ=255)
                                                                                                        acaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAat                                         <  1:4069722/150‑1 (MQ=255)
                                                                                                        acaTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAat                                         <  1:3831078/150‑1 (MQ=255)
                                                                                                         caTCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAata                                        >  2:513094/1‑150 (MQ=255)
                                                                                                            ccTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCt                                     >  2:3518687/1‑150 (MQ=255)
                                                                                                             cTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCtt                                    <  1:68518/150‑1 (MQ=255)
                                                                                                               ggCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTa                                  >  1:3403879/1‑150 (MQ=255)
                                                                                                               ggCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGACTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTa                                  >  2:4372516/1‑150 (MQ=255)
                                                                                                               ggCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCTAATATCTTTa                                  >  2:425492/1‑150 (MQ=255)
                                                                                                                gCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTaa                                 <  2:2023107/150‑1 (MQ=255)
                                                                                                                gCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTaa                                 >  1:1820456/1‑150 (MQ=255)
                                                                                                                gCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTaa                                 >  1:2742444/1‑150 (MQ=255)
                                                                                                                 cTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCTAATATCTTTAAt                                <  1:4456036/150‑1 (MQ=255)
                                                                                                                  tCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATa                               <  1:3255911/150‑1 (MQ=255)
                                                                                                                  tCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCTAATATCTTTAATa                               <  1:2001864/150‑1 (MQ=255)
                                                                                                                   cATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAg                              <  1:3810138/150‑1 (MQ=255)
                                                                                                                   cATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAg                              <  1:3810217/150‑1 (MQ=255)
                                                                                                                    aTAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGc                             <  2:1014867/150‑1 (MQ=255)
                                                                                                                     tAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCa                            >  1:2892994/1‑150 (MQ=255)
                                                                                                                       aaGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCAcc                          >  2:3630877/1‑150 (MQ=255)
                                                                                                                           aaaTTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATa                      <  1:526700/150‑1 (MQ=255)
                                                                                                                           aaaTTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATa                      >  2:2274165/1‑150 (MQ=255)
                                                                                                                            aaTTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATaa                     >  1:2845644/1‑150 (MQ=255)
                                                                                                                              ttATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAcc                   >  2:3029786/1‑150 (MQ=255)
                                                                                                                              ttATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAcc                   >  2:3396137/1‑150 (MQ=255)
                                                                                                                               tATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAcca                  >  2:2938156/1‑150 (MQ=255)
                                                                                                                                aTCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccag                 <  2:2845644/150‑1 (MQ=255)
                                                                                                                                 tCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagc                >  2:4527028/1‑150 (MQ=255)
                                                                                                                                 tCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAccagc                >  2:1844692/1‑150 (MQ=255)
                                                                                                                                    aTAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCTAATATCTTTAATAGCACCAATAAccagccag             >  2:1192173/1‑150 (MQ=255)
                                                                                                                                       aGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGc          >  1:4002596/1‑150 (MQ=255)
                                                                                                                                         aTAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAg        >  2:1678416/1‑150 (MQ=255)
                                                                                                                                          tAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGa       >  1:2559505/1‑150 (MQ=255)
                                                                                                                                              cTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGAttgt   >  1:3432969/1‑150 (MQ=255)
                                                                                                                                              cTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGAttgt   >  2:190788/1‑150 (MQ=255)
                                                                                                                                              cTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGAttgt   <  1:625937/150‑1 (MQ=255)
                                                                                                                                               tGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGAttgtt  >  1:2601598/1‑150 (MQ=255)
                                                                                                                                               tGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGAttgtt  <  2:1614866/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTT  >  CP009273/557637‑557929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTTGTT  >  CP009273/557635‑557932
                                                                                                                                                     |                                                                                                                                                    
TACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAT                                                                                                                                                      <  SRR19973076.2307750/150‑1 (MQ=60)
TACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCAATGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAT                                                                                                                                                      <  SRR19973076.2307738/150‑1 (MQ=60)
  CTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGAT                                                                                                                                                    <  SRR19973076.3273334/150‑1 (MQ=60)
        CAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTAT                                                                                                                                              <  SRR19973076.864184/150‑1 (MQ=60)
         AGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATT                                                                                                                                             <  SRR19973076.4184571/150‑1 (MQ=60)
         AGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATT                                                                                                                                             <  SRR19973076.4184862/150‑1 (MQ=60)
         AGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATT                                                                                                                                             <  SRR19973076.4184812/150‑1 (MQ=60)
          GTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTG                                                                                                                                            >  SRR19973076.2026102/1‑150 (MQ=60)
          GTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTG                                                                                                                                            >  SRR19973076.2026074/1‑150 (MQ=60)
          GTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTG                                                                                                                                            >  SRR19973076.4459016/1‑150 (MQ=60)
            GGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGC                                                                                                                                          <  SRR19973076.2313734/150‑1 (MQ=60)
                AGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATG                                                                                                                                      <  SRR19973076.833428/150‑1 (MQ=60)
                 GACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGG                                                                                                                                     >  SRR19973076.2960527/1‑150 (MQ=60)
                 GACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGG                                                                                                                                     >  SRR19973076.1353677/1‑150 (MQ=60)
                  ACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGC                                                                                                                                    <  SRR19973076.2774711/150‑1 (MQ=60)
                  ACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGC                                                                                                                                    <  SRR19973076.2774740/150‑1 (MQ=60)
                    GGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCAT                                                                                                                                  >  SRR19973076.4107087/1‑150 (MQ=60)
                    GGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCAT                                                                                                                                  >  SRR19973076.4107126/1‑150 (MQ=60)
                    GGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCAT                                                                                                                                  <  SRR19973076.2874449/150‑1 (MQ=60)
                    GGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCAT                                                                                                                                  >  SRR19973076.1883795/1‑150 (MQ=60)
                    GGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCGGCATATTATTATTGGCGATGGCAT                                                                                                                                  <  SRR19973076.3692754/150‑1 (MQ=60)
                        CGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTT                                                                                                                              >  SRR19973076.4240975/1‑150 (MQ=60)
                        CGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTT                                                                                                                              >  SRR19973076.3510627/1‑150 (MQ=60)
                        CGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTT                                                                                                                              >  SRR19973076.1235238/1‑150 (MQ=60)
                        CGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTT                                                                                                                              >  SRR19973076.1235682/1‑150 (MQ=60)
                        CGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTT                                                                                                                              >  SRR19973076.1235227/1‑150 (MQ=60)
                         GAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTG                                                                                                                             <  SRR19973076.2038195/150‑1 (MQ=60)
                         GAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTG                                                                                                                             >  SRR19973076.2614533/1‑150 (MQ=60)
                            CTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGT                                                                                                                          >  SRR19973076.2502622/1‑150 (MQ=60)
                                     CATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAAC                                                                                                                 >  SRR19973076.3786305/1‑150 (MQ=60)
                                     CATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAAC                                                                                                                 >  SRR19973076.3786397/1‑150 (MQ=60)
                                       TTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACAT                                                                                                               <  SRR19973076.45386/150‑1 (MQ=60)
                                        TGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATA                                                                                                              >  SRR19973076.1076074/1‑150 (MQ=60)
                                        TGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATA                                                                                                              >  SRR19973076.906210/1‑150 (MQ=60)
                                         GCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAATATAATCTGCATATTATTATAGGCGATGTCATGTTTGTGTCTGGCAAACATAT                                                                                                             >  SRR19973076.4432096/1‑150 (MQ=60)
                                           GCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCC                                                                                                           <  SRR19973076.123575/150‑1 (MQ=60)
                                              CTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGG                                                                                                        >  SRR19973076.612737/1‑150 (MQ=60)
                                              CTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGG                                                                                                        >  SRR19973076.612742/1‑150 (MQ=60)
                                                ATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGC                                                                                                      >  SRR19973076.970003/1‑150 (MQ=60)
                                                ATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGC                                                                                                      >  SRR19973076.1252092/1‑150 (MQ=60)
                                                ATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGC                                                                                                      >  SRR19973076.1252114/1‑150 (MQ=60)
                                                ATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGC                                                                                                      >  SRR19973076.1252123/1‑150 (MQ=60)
                                                ATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGC                                                                                                      >  SRR19973076.1252184/1‑150 (MQ=60)
                                                ATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGC                                                                                                      >  SRR19973076.1252652/1‑150 (MQ=60)
                                                  GTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTA                                                                                                    <  SRR19973076.3811547/150‑1 (MQ=60)
                                                        CCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTT                                                                                              <  SRR19973076.21286/150‑1 (MQ=60)
                                                        CCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTT                                                                                              <  SRR19973076.2181867/150‑1 (MQ=60)
                                                         CGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTG                                                                                             <  SRR19973076.272669/150‑1 (MQ=60)
                                                               AAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAA                                                                                       >  SRR19973076.4388310/1‑150 (MQ=60)
                                                               AAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAA                                                                                       >  SRR19973076.2601239/1‑150 (MQ=60)
                                                                AAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAAT                                                                                      >  SRR19973076.4378768/1‑150 (MQ=60)
                                                                 AGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATA                                                                                     >  SRR19973076.4072667/1‑150 (MQ=60)
                                                                 AGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATA                                                                                     >  SRR19973076.4073004/1‑150 (MQ=60)
                                                                   CTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGT                                                                                   >  SRR19973076.2622072/1‑150 (MQ=60)
                                                                    TTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTA                                                                                  >  SRR19973076.4136014/1‑150 (MQ=60)
                                                                    TTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTA                                                                                  >  SRR19973076.1882084/1‑150 (MQ=60)
                                                                    TTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTA                                                                                  >  SRR19973076.1882097/1‑150 (MQ=60)
                                                                     TACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTAC                                                                                 <  SRR19973076.4231512/150‑1 (MQ=60)
                                                                     TACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTAC                                                                                 <  SRR19973076.4232103/150‑1 (MQ=60)
                                                                        AGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGG                                                                              >  SRR19973076.2691951/1‑150 (MQ=60)
                                                                           AAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGT                                                                           <  SRR19973076.1846743/150‑1 (MQ=60)
                                                                           AAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGT                                                                           >  SRR19973076.3662266/1‑150 (MQ=60)
                                                                           AAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGT                                                                           >  SRR19973076.4023570/1‑150 (MQ=60)
                                                                           AAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGT                                                                           >  SRR19973076.4022057/1‑150 (MQ=60)
                                                                            AGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTA                                                                          >  SRR19973076.1057801/1‑150 (MQ=60)
                                                                            AGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTA                                                                          >  SRR19973076.3396225/1‑150 (MQ=60)
                                                                               CGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCA                                                                       >  SRR19973076.1716235/1‑150 (MQ=60)
                                                                                GTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAG                                                                      >  SRR19973076.4523339/1‑150 (MQ=60)
                                                                                GTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAG                                                                      >  SRR19973076.4354294/1‑150 (MQ=60)
                                                                                    CTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGA                                                                  >  SRR19973076.1631344/1‑150 (MQ=60)
                                                                                     TGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGAT                                                                 >  SRR19973076.3290391/1‑150 (MQ=60)
                                                                                     TGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGAT                                                                 <  SRR19973076.3982327/150‑1 (MQ=60)
                                                                                         ATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAAC                                                             >  SRR19973076.2318363/1‑150 (MQ=60)
                                                                                         ATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAAC                                                             >  SRR19973076.2318369/1‑150 (MQ=60)
                                                                                           TTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACAC                                                           <  SRR19973076.936269/150‑1 (MQ=60)
                                                                                            TCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACT                                                          <  SRR19973076.1481439/150‑1 (MQ=60)
                                                                                             CGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTA                                                         >  SRR19973076.96003/1‑150 (MQ=60)
                                                                                               GCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATG                                                       <  SRR19973076.339351/150‑1 (MQ=60)
                                                                                               GCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATG                                                       <  SRR19973076.339428/150‑1 (MQ=60)
                                                                                                 AGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAT                                                     >  SRR19973076.2131054/1‑150 (MQ=60)
                                                                                                 AGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAT                                                     >  SRR19973076.581059/1‑150 (MQ=60)
                                                                                                 AGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGAT                                                     >  SRR19973076.581073/1‑150 (MQ=60)
                                                                                                   TTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTTTTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCT                                                   <  SRR19973076.3906116/150‑1 (MQ=60)
                                                                                                   TTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCT                                                   <  SRR19973076.3904046/150‑1 (MQ=60)
                                                                                                    TTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTC                                                  >  SRR19973076.749314/1‑150 (MQ=60)
                                                                                                    TTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTC                                                  >  SRR19973076.749317/1‑150 (MQ=60)
                                                                                                     TGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCT                                                 >  SRR19973076.3008505/1‑150 (MQ=60)
                                                                                                      GCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCTTGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTC                                                <  SRR19973076.4443235/150‑1 (MQ=60)
                                                                                                      GCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTC                                                <  SRR19973076.860745/150‑1 (MQ=60)
                                                                                                         CACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAA                                             <  SRR19973076.766017/150‑1 (MQ=60)
                                                                                                          ACATCCTGGCTCATAAAGGAAATTATCAATACGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAAT                                            <  SRR19973076.3836620/150‑1 (MQ=60)
                                                                                                          ACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAAT                                            <  SRR19973076.3837814/150‑1 (MQ=60)
                                                                                                          ACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAAT                                            <  SRR19973076.3836715/150‑1 (MQ=60)
                                                                                                          ACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAAT                                            <  SRR19973076.4075736/150‑1 (MQ=60)
                                                                                                          ACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAAT                                            <  SRR19973076.3836598/150‑1 (MQ=60)
                                                                                                               CTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTT                                       <  SRR19973076.68667/150‑1 (MQ=60)
                                                                                                               CTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTT                                       <  SRR19973076.68359/150‑1 (MQ=60)
                                                                                                                 GGCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTA                                     >  SRR19973076.3408808/1‑150 (MQ=60)
                                                                                                                  GCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAA                                    >  SRR19973076.2746427/1‑150 (MQ=60)
                                                                                                                  GCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAA                                    >  SRR19973076.2747746/1‑150 (MQ=60)
                                                                                                                  GCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAA                                    >  SRR19973076.1824324/1‑150 (MQ=60)
                                                                                                                  GCTCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAA                                    >  SRR19973076.1823068/1‑150 (MQ=60)
                                                                                                                   CTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCTAATATCTTTAAT                                   <  SRR19973076.4462669/150‑1 (MQ=60)
                                                                                                                    TCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATA                                  <  SRR19973076.3260660/150‑1 (MQ=60)
                                                                                                                    TCATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATA                                  <  SRR19973076.3260640/150‑1 (MQ=60)
                                                                                                                    TCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCTAATATCTTTAATA                                  <  SRR19973076.2004789/150‑1 (MQ=60)
                                                                                                                     CATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAG                                 <  SRR19973076.3815791/150‑1 (MQ=60)
                                                                                                                     CATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAG                                 <  SRR19973076.3815732/150‑1 (MQ=60)
                                                                                                                     CATAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAG                                 <  SRR19973076.3815811/150‑1 (MQ=60)
                                                                                                                     CATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAG                                 >  SRR19973076.3415960/1‑150 (MQ=60)
                                                                                                                       TAAAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCA                               >  SRR19973076.1468065/1‑150 (MQ=60)
                                                                                                                       TAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCA                               >  SRR19973076.2897191/1‑150 (MQ=60)
                                                                                                                         AAGGAAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCTGAAGATGAACACTATGATCTCTCAATTATCTTTAATAGCACC                             <  SRR19973076.4439568/150‑1 (MQ=60)
                                                                                                                            GAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCTAATATCTTTAATAGCACCAAT                          <  SRR19973076.3446228/150‑1 (MQ=60)
                                                                                                                             AAATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATA                         <  SRR19973076.527537/150‑1 (MQ=60)
                                                                                                                              AATTATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAA                        >  SRR19973076.2849757/1‑150 (MQ=60)
                                                                                                                               ATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATATCACCAATAAC                       >  SRR19973076.3287552/1‑150 (MQ=60)
                                                                                                                               ATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAC                       >  SRR19973076.3287551/1‑150 (MQ=60)
                                                                                                                                  ATCAATAAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAG                    >  SRR19973076.337834/1‑150 (MQ=60)
                                                                                                                                  ATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCTAATATCTTTAATAGCACCAATAACCAG                    <  SRR19973076.1999303/150‑1 (MQ=60)
                                                                                                                                        AAGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGG              >  SRR19973076.2794943/1‑150 (MQ=60)
                                                                                                                                         AGATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGC             >  SRR19973076.4008500/1‑150 (MQ=60)
                                                                                                                                          GATAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCA            <  SRR19973076.1938577/150‑1 (MQ=60)
                                                                                                                                            TAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGA          >  SRR19973076.2563204/1‑150 (MQ=60)
                                                                                                                                            TAATCTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGA          >  SRR19973076.2563230/1‑150 (MQ=60)
                                                                                                                                                CTGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGT      >  SRR19973076.3437942/1‑150 (MQ=60)
                                                                                                                                                CTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGT      <  SRR19973076.1072660/150‑1 (MQ=60)
                                                                                                                                                CTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGT      <  SRR19973076.626896/150‑1 (MQ=60)
                                                                                                                                                 TGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTT     >  SRR19973076.2605377/1‑150 (MQ=60)
                                                                                                                                                 TGCATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTT     >  SRR19973076.2605388/1‑150 (MQ=60)
                                                                                                                                                    ATATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAAACAGCCAGGGCAGATTGTTGTT  >  SRR19973076.3234831/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTACAGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAAAGGAAATTATCAATAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTACTGTTTGTGCAAATAGTACTGGCGTAGCAGAAGATGAACACTATGATCTCTCAAATATCTTTAATAGCACCAATAACCAGCCAGGGCAGATTGTTGTT  >  CP009273/557635‑557932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑