Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,747,034 C→A 12.9% D352Y (GAT→TAT)  ydhV ← putative oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,747,0340CA12.9% 902.1 / 74.5 264D352Y (GAT→TAT) ydhVputative oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base C (103/127);  new base A (19/15);  total (122/142)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 268 total aligned reads displayed.

TGGCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTA  >  CP009273/1746885‑1747183
                                                                                                                                                     |                                                                                                                                                     
    aGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTc                                                                                                                                                   >  2:3552937/1‑150 (MQ=255)
    aGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTc                                                                                                                                                   >  2:3552849/1‑150 (MQ=255)
       acacGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGc                                                                                                                                                >  2:4092394/1‑150 (MQ=255)
         acGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGccc                                                                                                                                              >  2:1824701/1‑150 (MQ=255)
         acGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGACGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACAATCGTCTTTATCTTCAAAATATTTCGGccc                                                                                                                                              >  1:2495025/1‑150 (MQ=255)
          cGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCg                                                                                                                                             <  2:1110500/150‑1 (MQ=255)
          cGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCg                                                                                                                                             >  2:3153342/1‑150 (MQ=255)
           gCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGt                                                                                                                                            <  2:1291963/150‑1 (MQ=255)
           gCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGt                                                                                                                                            >  1:2247142/1‑150 (MQ=255)
              tGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCg                                                                                                                                         >  2:3250999/1‑150 (MQ=255)
               gAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCgg                                                                                                                                        >  2:4155714/1‑150 (MQ=255)
               gAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCgg                                                                                                                                        >  1:2524636/1‑150 (MQ=255)
                 acacacCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGa                                                                                                                                      >  1:68021/1‑150 (MQ=255)
                  cacacCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGaa                                                                                                                                     <  1:2011465/150‑1 (MQ=255)
                              gTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATg                                                                                                                         <  1:450148/150‑1 (MQ=255)
                              gTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATg                                                                                                                         >  2:1140971/1‑150 (MQ=255)
                                  cAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACa                                                                                                                     <  1:2802383/150‑1 (MQ=255)
                                  cAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACa                                                                                                                     <  1:3109310/150‑1 (MQ=255)
                                   aaTAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACaa                                                                                                                    >  2:3368323/1‑150 (MQ=255)
                                        gTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGttt                                                                                                               <  1:533170/150‑1 (MQ=255)
                                           aaGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATTGACAAAGTTTGCt                                                                                                            >  1:3114815/1‑150 (MQ=255)
                                           aaGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCt                                                                                                            <  1:4027825/150‑1 (MQ=255)
                                           aaGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCt                                                                                                            <  1:4185573/150‑1 (MQ=255)
                                           aaGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCt                                                                                                            >  1:1371981/1‑150 (MQ=255)
                                           aaGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCt                                                                                                            >  1:147442/1‑150 (MQ=255)
                                            aGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTa                                                                                                           >  2:836028/1‑150 (MQ=255)
                                            aGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTa                                                                                                           <  1:2354217/150‑1 (MQ=255)
                                               cgcgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTacac                                                                                                        >  1:3938110/1‑150 (MQ=255)
                                                  gATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAg                                                                                                     <  2:1866470/150‑1 (MQ=255)
                                                   aTGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAgt                                                                                                    <  2:2018951/150‑1 (MQ=255)
                                                    tGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAgtg                                                                                                   >  2:490887/1‑150 (MQ=255)
                                                       aaCTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACCAGTGTTa                                                                                                >  2:4216127/1‑150 (MQ=255)
                                                       aaCTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTa                                                                                                >  1:3409590/1‑150 (MQ=255)
                                                              cGTAGTTGCACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtg                                                                                         >  2:2803851/1‑150 (MQ=255)
                                                              cGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtg                                                                                         >  1:1670699/1‑150 (MQ=255)
                                                               gTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtgt                                                                                        >  1:171882/1‑150 (MQ=255)
                                                                tAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtgtg                                                                                       <  1:2781431/150‑1 (MQ=255)
                                                                tAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtgtg                                                                                       <  2:1062897/150‑1 (MQ=255)
                                                                tAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtgtg                                                                                       >  2:1375382/1‑150 (MQ=255)
                                                                   ttACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTg                                                                                    >  1:2127401/1‑150 (MQ=255)
                                                                      cacCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGc                                                                                 >  1:786952/1‑150 (MQ=255)
                                                                         cATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACg                                                                              >  2:3651144/1‑150 (MQ=255)
                                                                         cATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACg                                                                              <  1:227758/150‑1 (MQ=255)
                                                                            aGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCa                                                                           >  1:1892733/1‑150 (MQ=255)
                                                                            aGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCa                                                                           <  2:1169639/150‑1 (MQ=255)
                                                                             ggCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCaa                                                                          <  2:611753/150‑1 (MQ=255)
                                                                             ggCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCaa                                                                          <  1:2078147/150‑1 (MQ=255)
                                                                              gCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCaaa                                                                         <  2:2189987/150‑1 (MQ=255)
                                                                               ccGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAAc                                                                        <  1:3569184/150‑1 (MQ=255)
                                                                                cGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAAct                                                                       <  1:1699535/150‑1 (MQ=255)
                                                                                cGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAAct                                                                       >  1:4386226/1‑150 (MQ=255)
                                                                                 gTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAActc                                                                      >  2:44622/1‑150 (MQ=255)
                                                                                 gTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAActc                                                                      <  2:906453/150‑1 (MQ=255)
                                                                                    gTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCttt                                                                   <  2:734060/150‑1 (MQ=255)
                                                                                      cATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCa                                                                 <  2:4328434/150‑1 (MQ=255)
                                                                                      cATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCa                                                                 <  1:3826229/150‑1 (MQ=255)
                                                                                       aTCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAc                                                                >  2:3804739/1‑150 (MQ=255)
                                                                                        tCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAcc                                                               <  2:2903243/150‑1 (MQ=255)
                                                                                        tCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAcc                                                               <  2:211336/150‑1 (MQ=255)
                                                                                           aaCAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGa                                                            <  1:92476/150‑1 (MQ=255)
                                                                                           aaCAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGGTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGa                                                            >  1:3994381/1‑150 (MQ=255)
                                                                                                aTTCAGACCCACCAGGTTACCCATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAat                                                       >  2:2372953/1‑150 (MQ=255)
                                                                                                 ttCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAata                                                      <  2:4426102/150‑1 (MQ=255)
                                                                                                 ttCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAata                                                      <  1:1843574/150‑1 (MQ=255)
                                                                                                     gACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCa                                                  <  1:3860961/150‑1 (MQ=255)
                                                                                                       cccACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCAtt                                                <  2:2553202/150‑1 (MQ=255)
                                                                                                        ccaccaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCAttt                                               <  2:1718377/150‑1 (MQ=255)
                                                                                                        ccaccaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCAttt                                               <  2:3098242/150‑1 (MQ=255)
                                                                                                         caccaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTg                                              <  1:74493/150‑1 (MQ=255)
                                                                                                         caccaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTg                                              >  2:3415709/1‑150 (MQ=255)
                                                                                                          accaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTgg                                             <  2:1670699/150‑1 (MQ=255)
                                                                                                              ggTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCa                                         <  1:432286/150‑1 (MQ=255)
                                                                                                              ggTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCa                                         >  1:2291720/1‑150 (MQ=255)
                                                                                                                ttACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATa                                       >  1:1636308/1‑150 (MQ=255)
                                                                                                                ttACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGATACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATa                                       <  2:513755/150‑1 (MQ=255)
                                                                                                                 tACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATAc                                      <  2:3313576/150‑1 (MQ=255)
                                                                                                                     aaTCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACg                                  >  1:295283/1‑150 (MQ=255)
                                                                                                                      aTCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACgg                                 >  1:1162312/1‑150 (MQ=255)
                                                                                                                        cacaCGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGAt                               <  2:4595172/150‑1 (MQ=255)
                                                                                                                         acacGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGGTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATc                              <  1:198293/150‑1 (MQ=255)
                                                                                                                          cacGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCg                             <  1:332259/150‑1 (MQ=255)
                                                                                                                             gACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGc                          <  2:716368/150‑1 (MQ=255)
                                                                                                                             gACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGc                          >  1:311707/1‑150 (MQ=255)
                                                                                                                             gACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGc                          <  2:1685716/150‑1 (MQ=255)
                                                                                                                                cATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAag                       <  2:3857977/150‑1 (MQ=255)
                                                                                                                                 aTCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAaga                      <  1:339123/150‑1 (MQ=255)
                                                                                                                                 aTCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAaga                      >  1:4477616/1‑150 (MQ=255)
                                                                                                                                   cGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGt                    <  2:2622590/150‑1 (MQ=255)
                                                                                                                                   cGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGt                    >  2:1641866/1‑150 (MQ=255)
                                                                                                                                    gTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTg                   >  2:1385961/1‑150 (MQ=255)
                                                                                                                                    gTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTg                   >  2:2974027/1‑150 (MQ=255)
                                                                                                                                       tttATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCa                <  2:292592/150‑1 (MQ=255)
                                                                                                                                         tATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGc              >  2:316886/1‑150 (MQ=255)
                                                                                                                                          aTCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGcc             <  1:265381/150‑1 (MQ=255)
                                                                                                                                          aTCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGcc             <  2:3326219/150‑1 (MQ=255)
                                                                                                                                          aTCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGcc             >  2:2711782/1‑150 (MQ=255)
                                                                                                                                           tCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCg            <  2:1562034/150‑1 (MQ=255)
                                                                                                                                            cTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGc           >  1:1064815/1‑150 (MQ=255)
                                                                                                                                             ttCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACTGATCGGGCAAGAGTGGCAGCCGCt          >  2:102380/1‑150 (MQ=255)
                                                                                                                                              tCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTc         <  1:425821/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTA  >  CP009273/1746885‑1747183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A