Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F181 I0 R1	1262	323.1	10297870	97.5%	10040423	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,747,034	C→A	21.1%	D352Y (GAT→TAT)	ydhV ←	putative oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,747,034	0	C	A	21.1%	782.7 / 152.0	288	D352Y (GAT→TAT)	ydhV	putative oxidoreductase subunit
Reads supporting (aligned to +/- strand): ref base C (117/110); new base A (28/33); total (145/143)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TGGCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTT  >  CP009273/1746885‑1747182

  gCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATAtt                                                                                                                                                    <  1:3326852/150‑1 (MQ=255)

    aGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTc                                                                                                                                                  >  1:4020417/1‑150 (MQ=255)

    aGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTc                                                                                                                                                  <  1:5139679/150‑1 (MQ=255)

     gAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCg                                                                                                                                                 <  1:4256641/150‑1 (MQ=255)

        cacGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGcc                                                                                                                                              <  1:1096031/150‑1 (MQ=255)

        cacGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGcc                                                                                                                                              >  2:568165/1‑150 (MQ=255)

        cacGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGcc                                                                                                                                              >  2:4498736/1‑150 (MQ=255)

           gCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGt                                                                                                                                           <  2:4855363/150‑1 (MQ=255)

           gCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGt                                                                                                                                           >  1:4983223/1‑150 (MQ=255)

             ttGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTc                                                                                                                                         >  1:4567169/1‑150 (MQ=255)

             ttGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTc                                                                                                                                         <  1:4867603/150‑1 (MQ=255)

              tGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCg                                                                                                                                        <  1:2966957/150‑1 (MQ=255)

               gAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCgg                                                                                                                                       <  2:3790546/150‑1 (MQ=255)

                 acacacCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGCCCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGGTCGGGa                                                                                                                                     <  2:3543507/150‑1 (MQ=255)

                   acacCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAg                                                                                                                                   <  1:2815128/150‑1 (MQ=255)

                     acCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGAt                                                                                                                                 >  2:1363103/1‑150 (MQ=255)

                       cTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGAtgg                                                                                                                               >  1:4949305/1‑150 (MQ=255)

                        tttGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGAtggt                                                                                                                              >  1:2849496/1‑150 (MQ=255)

                         ttGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGAtggtg                                                                                                                             <  1:1950243/150‑1 (MQ=255)

                          tGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGAtggtgg                                                                                                                            >  2:3619773/1‑150 (MQ=255)

                          tGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGAtggtgg                                                                                                                            >  2:43698/1‑150 (MQ=255)

                                 aCAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGAc                                                                                                                     <  1:3391618/150‑1 (MQ=255)

                                 aCAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGAc                                                                                                                     >  2:2034989/1‑150 (MQ=255)

                                 aCAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGAc                                                                                                                     >  2:1414654/1‑150 (MQ=255)

                                  cAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACa                                                                                                                    >  2:3301746/1‑150 (MQ=255)

                                     tAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTCTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAg                                                                                                                 <  2:2529901/150‑1 (MQ=255)

                                      aaGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGt                                                                                                                >  1:2298295/1‑150 (MQ=255)

                                       aGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGtt                                                                                                               <  1:3610868/150‑1 (MQ=255)

                                         tAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTg                                                                                                             >  2:2436597/1‑150 (MQ=255)

                                            aGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTa                                                                                                          >  2:1184069/1‑150 (MQ=255)

                                            aGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTa                                                                                                          >  2:1591941/1‑150 (MQ=255)

                                             gTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTac                                                                                                         <  2:2612954/150‑1 (MQ=255)

                                               cgcgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTacac                                                                                                       >  1:3821403/1‑150 (MQ=255)

                                               cgcgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTacac                                                                                                       >  1:4118280/1‑150 (MQ=255)

                                               cgcgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTacac                                                                                                       >  1:4118283/1‑150 (MQ=255)

                                               cgcgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTacac                                                                                                       >  1:4118307/1‑150 (MQ=255)

                                               cgcgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTacac                                                                                                       >  2:1158194/1‑150 (MQ=255)

                                                gcgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTAcaca                                                                                                      <  1:2503876/150‑1 (MQ=255)

                                                  gATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAg                                                                                                    >  1:938769/1‑150 (MQ=255)

                                                  gATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAg                                                                                                    >  1:1008761/1‑150 (MQ=255)

                                                     gCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAgtgt                                                                                                 <  1:4229174/150‑1 (MQ=255)

                                                      cAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGtt                                                                                                >  1:3747948/1‑150 (MQ=255)

                                                          tGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTacca                                                                                            <  2:952970/150‑1 (MQ=255)

                                                             ccGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCt                                                                                         >  2:772371/1‑150 (MQ=255)

                                                               gTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtgt                                                                                       >  2:4032931/1‑150 (MQ=255)

                                                               gTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtgt                                                                                       >  1:880744/1‑150 (MQ=255)

                                                                   ttACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGATTGCTAAACAAGTGTTACCACCTGTGCTg                                                                                   >  2:1350460/1‑150 (MQ=255)

                                                                            aGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCa                                                                          <  1:241508/150‑1 (MQ=255)

                                                                            aGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCa                                                                          >  1:2079487/1‑150 (MQ=255)

                                                                            aGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCa                                                                          <  2:2804861/150‑1 (MQ=255)

                                                                              gCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCaaa                                                                        <  2:5110981/150‑1 (MQ=255)

                                                                              gCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCaaa                                                                        <  1:2649429/150‑1 (MQ=255)

                                                                               ccGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAAc                                                                       <  2:5012320/150‑1 (MQ=255)

                                                                                cGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAAct                                                                      >  1:2686539/1‑150 (MQ=255)

                                                                                  tAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAActct                                                                    <  1:58073/150‑1 (MQ=255)

                                                                                  tAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAActct                                                                    <  1:3162380/150‑1 (MQ=255)

                                                                                   aGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCtt                                                                   >  1:1128962/1‑150 (MQ=255)

                                                                                     tCATCGAACAAATTCAGACCCCCCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTc                                                                 <  2:445276/150‑1 (MQ=255)

                                                                                     tCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTc                                                                 <  2:4382259/150‑1 (MQ=255)

                                                                                     tCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTc                                                                 <  2:839869/150‑1 (MQ=255)

                                                                                     tCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTc                                                                 <  1:5086788/150‑1 (MQ=255)

                                                                                       aTCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAc                                                               <  2:282698/150‑1 (MQ=255)

                                                                                       aTCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAc                                                               >  1:2113284/1‑150 (MQ=255)

                                                                                       aTCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAc                                                               <  2:1656986/150‑1 (MQ=255)

                                                                                       aTCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAACATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAc                                                               >  1:4248102/1‑150 (MQ=255)

                                                                                           aaCAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGa                                                           <  1:1596209/150‑1 (MQ=255)

                                                                                           aaCAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGa                                                           >  1:1995133/1‑150 (MQ=255)

                                                                                            aCAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAg                                                          >  1:1646505/1‑150 (MQ=255)

                                                                                              aaaTTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGa                                                        >  2:1765112/1‑150 (MQ=255)

                                                                                                aTTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAat                                                      <  1:3484392/150‑1 (MQ=255)

                                                                                                  tCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAatat                                                    >  2:2095952/1‑150 (MQ=255)

                                                                                                     gACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCa                                                 >  2:1786824/1‑150 (MQ=255)

                                                                                                     gACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCa                                                 <  2:300348/150‑1 (MQ=255)

                                                                                                      aCCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCAt                                                <  2:3708202/150‑1 (MQ=255)

                                                                                                      aCCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCAt                                                >  1:3646512/1‑150 (MQ=255)

                                                                                                        ccaccaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATCTTCAttt                                              >  2:2912405/1‑150 (MQ=255)

                                                                                                             aGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTc                                         >  1:1630267/1‑150 (MQ=255)

                                                                                                              ggTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCa                                        >  1:1880750/1‑150 (MQ=255)

                                                                                                                 taacAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATAAATTTGGGTCATAc                                     >  1:467435/4‑150 (MQ=255)

                                                                                                                      aTCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGACTATTCATTTGGGTCATACAACgg                                >  2:2514886/1‑150 (MQ=255)

                                                                                                                       tCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGa                               >  1:1814903/1‑150 (MQ=255)

                                                                                                                        cacaCGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGAt                              >  1:2629348/1‑150 (MQ=255)

                                                                                                                           acGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATAGTCATTTGGGTCATACAACGGATCgg                           >  2:182513/1‑150 (MQ=255)

                                                                                                                                 aTCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAaga                     >  1:4935059/1‑150 (MQ=255)

                                                                                                                                    gTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTg                  <  2:4630392/150‑1 (MQ=255)

                                                                                                                                      cTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGc                <  2:4265078/150‑1 (MQ=255)

                                                                                                                                       tttATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCa               <  1:198830/150‑1 (MQ=255)

                                                                                                                                       tttATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCa               <  1:125937/150‑1 (MQ=255)

                                                                                                                                            cTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGc          >  1:447491/1‑150 (MQ=255)

                                                                                                                                            cTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGc          >  1:980228/1‑150 (MQ=255)

                                                                                                                                             ttCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCt         <  2:264113/150‑1 (MQ=255)

                                                                                                                                              tCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTc        >  2:1894450/1‑150 (MQ=255)

                                                                                                                                               cAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCa       <  2:2849496/150‑1 (MQ=255)

                                                                                                                                               cAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCa       >  1:1441257/1‑150 (MQ=255)

                                                                                                                                                aaaaTCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAt      <  1:2582441/150‑1 (MQ=255)

                                                                                                                                                aaaaTCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAt      <  1:2582195/150‑1 (MQ=255)

                                                                                                                                                 aaaTCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAtt     <  1:4698614/150‑1 (MQ=255)

                                                                                                                                                 aaaTATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAtt     <  1:5046651/150‑1 (MQ=255)

                                                                                                                                                  aaTATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAttt    <  2:2211305/150‑1 (MQ=255)

                                                                                                                                                  aaTATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAttt    <  2:1857826/150‑1 (MQ=255)

TGGCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTT  >  CP009273/1746885‑1747182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A