Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,747,034 C→A 14.7% D352Y (GAT→TAT)  ydhV ← putative oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,747,0340CA14.7% 912.7 / 92.4 280D352Y (GAT→TAT) ydhVputative oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base C (143/95);  new base A (28/14);  total (171/109)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 282 total aligned reads displayed.

TGGCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTT  >  CP009273/1746885‑1747182
                                                                                                                                                     |                                                                                                                                                    
tGGCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATc                                                                                                                                                      >  2:2306937/1‑150 (MQ=255)
 ggCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCt                                                                                                                                                     >  2:422741/1‑150 (MQ=255)
   cAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCttt                                                                                                                                                   <  1:3192118/150‑1 (MQ=255)
     gAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCg                                                                                                                                                 <  1:1516058/150‑1 (MQ=255)
     gAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCg                                                                                                                                                 <  2:3723410/150‑1 (MQ=255)
       acacGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGc                                                                                                                                               >  2:3695971/1‑150 (MQ=255)
            cTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGtt                                                                                                                                          <  2:637614/150‑1 (MQ=255)
            cTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCAGAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGtt                                                                                                                                          <  2:4102118/150‑1 (MQ=255)
             ttGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTc                                                                                                                                         >  1:3695134/1‑150 (MQ=255)
               gAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCgg                                                                                                                                       >  2:2621719/1‑150 (MQ=255)
               gAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCgg                                                                                                                                       <  2:532687/150‑1 (MQ=255)
                aaCACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCggg                                                                                                                                      <  2:2445416/150‑1 (MQ=255)
                  cacacCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGaa                                                                                                                                    >  2:3202502/1‑150 (MQ=255)
                  cacacCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGAAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGaa                                                                                                                                    >  1:3098415/1‑150 (MQ=255)
                   acacCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAg                                                                                                                                   >  2:2060595/1‑150 (MQ=255)
                   acacCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCATAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAg                                                                                                                                   <  2:412478/150‑1 (MQ=255)
                      ccTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGAtg                                                                                                                                >  1:1654791/1‑150 (MQ=255)
                      ccTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGAtg                                                                                                                                <  2:3885874/150‑1 (MQ=255)
                       cTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGAtgg                                                                                                                               <  1:4433155/150‑1 (MQ=255)
                       cTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGAtgg                                                                                                                               >  1:3424931/1‑150 (MQ=255)
                        tttGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGAtggt                                                                                                                              >  2:1523796/1‑150 (MQ=255)
                            cTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCAAAGGCCGTAGTCATCGAAAAAATTCAGACCCACCAGGTTAACAATCACACGAACATCGTCTATATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTa                                                                                                                          >  1:2605826/1‑150 (MQ=255)
                             tGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTAt                                                                                                                         <  2:674077/150‑1 (MQ=255)
                                aaCAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGa                                                                                                                      >  1:4263071/1‑150 (MQ=255)
                                aaCAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGa                                                                                                                      <  2:738054/150‑1 (MQ=255)
                                aaCAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGa                                                                                                                      >  2:2698489/1‑150 (MQ=255)
                                   aaTAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTCGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAATATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACaa                                                                                                                   >  2:2123341/1‑150 (MQ=255)
                                   aaTAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACaa                                                                                                                   <  1:4343263/150‑1 (MQ=255)
                                   aaTAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACaa                                                                                                                   >  1:846626/1‑150 (MQ=255)
                                      aaGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGt                                                                                                                >  1:1620099/1‑150 (MQ=255)
                                           aaGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCt                                                                                                           <  2:512964/150‑1 (MQ=255)
                                            aGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTa                                                                                                          <  1:2713295/150‑1 (MQ=255)
                                                gcgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTAcaca                                                                                                      >  2:1777399/1‑150 (MQ=255)
                                                 cgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACaa                                                                                                     >  1:1062177/1‑150 (MQ=255)
                                                 cgATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACaa                                                                                                     <  2:1070100/150‑1 (MQ=255)
                                                  gATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAg                                                                                                    >  2:1143595/1‑150 (MQ=255)
                                                  gATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAg                                                                                                    >  2:1848767/1‑150 (MQ=255)
                                                       aaCTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTa                                                                                               <  1:1412698/150‑1 (MQ=255)
                                                        aCTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTac                                                                                              <  1:2789608/150‑1 (MQ=255)
                                                           gCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTaccac                                                                                           >  2:3602181/1‑150 (MQ=255)
                                                            cccGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTaccacc                                                                                          <  1:744478/150‑1 (MQ=255)
                                                               gTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtgt                                                                                       >  1:3613837/1‑150 (MQ=255)
                                                                tAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCtgtg                                                                                      >  2:1478599/1‑150 (MQ=255)
                                                                    tACACCATAGGCCGGAGTCATCGACCAACTTCAGACCCACCCGGTTACCAATCACACGACCTTCGTCTTGCTCTTCAAACTCTGTCGGCCCCTTCGGGAAGATGGTGGTATGGACAACGTTTGCTACACAAGTGTTACCACCTGTGCTgg                                                                                  <  2:2784966/150‑1 (MQ=255)
                                                                           tAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGcc                                                                           >  1:4605540/1‑150 (MQ=255)
                                                                              gCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCaaa                                                                        >  1:2552388/1‑150 (MQ=255)
                                                                                  tAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAActct                                                                    >  1:3899124/1‑150 (MQ=255)
                                                                                   aGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCtt                                                                   <  1:2818983/150‑1 (MQ=255)
                                                                                    gTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCttt                                                                  <  2:431555/150‑1 (MQ=255)
                                                                                    gTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCttt                                                                  >  2:170616/1‑150 (MQ=255)
                                                                                      cATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCa                                                                <  2:2417688/150‑1 (MQ=255)
                                                                                        tCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAcc                                                              <  2:1970696/150‑1 (MQ=255)
                                                                                        tCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAcc                                                              <  2:3296338/150‑1 (MQ=255)
                                                                                        tCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAcc                                                              >  2:3386752/1‑150 (MQ=255)
                                                                                         cGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCAccc                                                             >  1:503658/1‑150 (MQ=255)
                                                                                          gAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCg                                                            >  2:40047/1‑150 (MQ=255)
                                                                                          gAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCg                                                            >  1:3629804/1‑150 (MQ=255)
                                                                                             cAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAgg                                                         >  2:437314/1‑150 (MQ=255)
                                                                                              aaaTTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGa                                                        >  1:213629/1‑150 (MQ=255)
                                                                                                   cAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATAtt                                                   <  1:1206721/150‑1 (MQ=255)
                                                                                                   cAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATAtt                                                   >  2:3822257/1‑150 (MQ=255)
                                                                                                   cAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATAtt                                                   >  2:2954275/1‑150 (MQ=255)
                                                                                                    aGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTc                                                  >  1:4176423/1‑150 (MQ=255)
                                                                                                    aGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTc                                                  <  2:1206240/150‑1 (MQ=255)
                                                                                                     gACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCa                                                 <  1:1840569/150‑1 (MQ=255)
                                                                                                        ccaccaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCAttt                                              <  1:4074007/150‑1 (MQ=255)
                                                                                                         caccaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTg                                             >  2:4474869/1‑150 (MQ=255)
                                                                                                          accaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTgg                                            >  1:993867/1‑150 (MQ=255)
                                                                                                           ccaGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGACTATTCATTTggg                                           >  1:2996049/1‑150 (MQ=255)
                                                                                                             aGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTc                                         >  2:877554/1‑150 (MQ=255)
                                                                                                             aGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTc                                         >  1:1879985/1‑150 (MQ=255)
                                                                                                               gTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCAt                                       <  1:3815546/150‑1 (MQ=255)
                                                                                                                        cacaCGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGAt                              >  1:4473321/1‑150 (MQ=255)
                                                                                                                          cacGACCATCGTCTTTATCTTCAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCg                            <  1:3832439/150‑1 (MQ=255)
                                                                                                                             gACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGc                         <  2:1401855/150‑1 (MQ=255)
                                                                                                                             gACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGc                         >  2:1895190/1‑150 (MQ=255)
                                                                                                                              aCCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCa                        <  2:1634329/150‑1 (MQ=255)
                                                                                                                               ccATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCaa                       >  1:3363578/1‑150 (MQ=255)
                                                                                                                                   cGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGt                   >  2:4581448/1‑150 (MQ=255)
                                                                                                                                   cGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGt                   >  2:1422317/1‑150 (MQ=255)
                                                                                                                                    gTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTg                  <  1:4579784/150‑1 (MQ=255)
                                                                                                                                    gTCTTTATCTTCAAAATCTGTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTg                  <  2:2888445/150‑1 (MQ=255)
                                                                                                                                    gTCTTTATCTTCAAAAACTTTCGGCCCGTTCTGGAAGATGGTGGTATGGTCAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATATGGGTCATACAACTGATCGGGCAAGAGTg                  >  2:2853406/1‑150 (MQ=255)
                                                                                                                                     tCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTgg                 >  1:2426050/1‑150 (MQ=255)
                                                                                                                                      cTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGc                >  2:1906190/1‑150 (MQ=255)
                                                                                                                                       tttATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCa               <  2:617564/150‑1 (MQ=255)
                                                                                                                                        ttATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGACTATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAg              >  2:3555399/1‑150 (MQ=255)
                                                                                                                                        ttATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAg              >  2:2542655/1‑150 (MQ=255)
                                                                                                                                          aTCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGcc            <  2:4182731/150‑1 (MQ=255)
                                                                                                                                          aTCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGcc            >  1:3340618/1‑150 (MQ=255)
                                                                                                                                          aTCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGcc            <  1:430670/150‑1 (MQ=255)
                                                                                                                                              tCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTc        <  2:246442/150‑1 (MQ=255)
                                                                                                                                               cAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCa       >  1:1608477/1‑150 (MQ=255)
                                                                                                                                               cAAAATATTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCa       >  1:1989443/1‑150 (MQ=255)
                                                                                                                                                 aaaTCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAtt     >  1:2476587/1‑150 (MQ=255)
                                                                                                                                                  aaTCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGACTATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAttt    >  2:3478699/1‑150 (MQ=255)
                                                                                                                                                  aaTCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAttt    >  2:696364/1‑150 (MQ=255)
                                                                                                                                                  aaTCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAttt    <  2:2254904/150‑1 (MQ=255)
                                                                                                                                                   aTCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGAAAACTTTTGCTACACAAGTGTTACCACCTGTGCGGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAtttt   <  2:500473/150‑1 (MQ=255)
                                                                                                                                                    tCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCAttttt  <  2:1476728/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGGCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAAGATGGTGGTATGGACAAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTT  >  CP009273/1746885‑1747182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A