Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,474,418 G→T 100% S413* (TCA→TAA)  emrY ← putative multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,474,4180GT99.6% 896.6 / ‑4.1 256S413* (TCA→TAA) emrYputative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (123/132);  total (123/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

GAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAA  >  CP009273/2474271‑2474564
                                                                                                                                                   |                                                                                                                                                  
gAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTAc                                                                                                                                                  <  2:1249140/150‑1 (MQ=255)
 aaaGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACAAGGGGTTAAATTGATCGATGGTAGCTGTCAACTGACTATGGTGTAACGATTAGCGTCGTCCCCACATCGTCATTGTCAACGACGTACCAACTGATCCTTACa                                                                                                                                                 >  2:37844/1‑150 (MQ=255)
  aaGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACaa                                                                                                                                                >  1:1199532/1‑150 (MQ=255)
  aaGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACaa                                                                                                                                                <  1:1849109/150‑1 (MQ=255)
     cGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGt                                                                                                                                             <  1:1583684/150‑1 (MQ=255)
      gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTa                                                                                                                                            <  2:3605189/150‑1 (MQ=255)
      gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTa                                                                                                                                            >  1:3386285/1‑150 (MQ=255)
       aaCCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTAc                                                                                                                                           <  2:3170620/150‑1 (MQ=255)
        aCCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACg                                                                                                                                          >  2:3365993/1‑150 (MQ=255)
            taataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaa                                                                                                                                      >  1:4523441/1‑150 (MQ=255)
              atatTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaaa                                                                                                                                    >  1:3791606/1‑150 (MQ=255)
               tatTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaaaa                                                                                                                                   >  1:4627940/1‑150 (MQ=255)
               tatTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaaaa                                                                                                                                   <  2:2695200/150‑1 (MQ=255)
                 tttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAtt                                                                                                                                 <  2:280451/150‑1 (MQ=255)
                 tttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAtt                                                                                                                                 <  1:3442951/150‑1 (MQ=255)
                  ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTa                                                                                                                                <  2:3473202/150‑1 (MQ=255)
                   tATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAc                                                                                                                               >  1:2340864/1‑150 (MQ=255)
                   tATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAc                                                                                                                               >  1:3479074/1‑150 (MQ=255)
                   tATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAc                                                                                                                               <  1:4206528/150‑1 (MQ=255)
                      ccATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCa                                                                                                                            <  1:2259828/150‑1 (MQ=255)
                       cATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCAt                                                                                                                           >  1:1056606/1‑150 (MQ=255)
                          aaTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACt                                                                                                                        <  1:4441250/150‑1 (MQ=255)
                           aTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTc                                                                                                                       >  2:4148643/1‑150 (MQ=255)
                            ttttTGTCGATTAATTAAACACGGGGTTAAATTGATCGTTGGTTGCTGTCAAGTGACTTTGGTGTATCGTTTCGCGTCGTCCCCACATCGTCATTGTCAACGACGTACCTACTGATCCGTACATGGTACGAAAAAAATTACTGATACTCg                                                                                                                      <  2:1978244/150‑1 (MQ=255)
                            tttGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCg                                                                                                                      >  2:3662761/1‑150 (MQ=255)
                                tgACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGc                                                                                                                  >  2:2698974/1‑150 (MQ=255)
                                 gACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCa                                                                                                                 <  2:1358585/150‑1 (MQ=255)
                                   cGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAtt                                                                                                               >  1:59053/1‑150 (MQ=255)
                                    gATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTg                                                                                                              >  2:1219554/1‑150 (MQ=255)
                                     aTGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTgg                                                                                                             <  2:3842713/150‑1 (MQ=255)
                                        aaTTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCaa                                                                                                          <  2:3701516/150‑1 (MQ=255)
                                            aaaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAttt                                                                                                      >  2:46585/1‑150 (MQ=255)
                                            aaaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAttt                                                                                                      <  2:4029473/150‑1 (MQ=255)
                                            aaaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAttt                                                                                                      <  2:2872893/150‑1 (MQ=255)
                                             aaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATtta                                                                                                     >  2:2981486/1‑150 (MQ=255)
                                              acacGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttat                                                                                                    >  2:3260439/1‑150 (MQ=255)
                                               cacGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatt                                                                                                   <  1:2073706/150‑1 (MQ=255)
                                                acGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatta                                                                                                  >  2:3020318/1‑150 (MQ=255)
                                                 cGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTtattat                                                                                                 >  1:4487326/1‑150 (MQ=255)
                                                    ggTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTg                                                                                              >  1:1464423/1‑150 (MQ=255)
                                                    ggTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTg                                                                                              >  1:4638474/1‑150 (MQ=255)
                                                      ttAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGc                                                                                            <  1:4291587/150‑1 (MQ=255)
                                                       tAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCa                                                                                           >  2:4017520/1‑150 (MQ=255)
                                                        aaaTTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCaa                                                                                          >  2:710315/1‑150 (MQ=255)
                                                        aaaTTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCaa                                                                                          <  1:1026127/150‑1 (MQ=255)
                                                         aaTTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAg                                                                                         >  2:911634/1‑150 (MQ=255)
                                                          aTTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                                                                                        >  1:3532115/1‑150 (MQ=255)
                                                          aTTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                                                                                        >  2:3624205/1‑150 (MQ=255)
                                                           ttGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGcc                                                                                       <  2:1256083/150‑1 (MQ=255)
                                                           ttGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGcc                                                                                       <  1:4311092/150‑1 (MQ=255)
                                                             gATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTg                                                                                     >  2:3978450/1‑150 (MQ=255)
                                                               tCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaa                                                                                   >  2:2409694/1‑150 (MQ=255)
                                                               tCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATGGGCAAATTTATTATCTGGCCAGCCTGaa                                                                                   >  2:3506186/1‑150 (MQ=255)
                                                                         cTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCg                                                                         <  1:2367226/150‑1 (MQ=255)
                                                                            tCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttg                                                                      <  2:2892917/150‑1 (MQ=255)
                                                                               aCTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTa                                                                   >  1:672104/1‑150 (MQ=255)
                                                                               aCTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTa                                                                   >  1:2862636/1‑150 (MQ=255)
                                                                                 tGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAg                                                                 <  2:1651325/150‑1 (MQ=255)
                                                                                  gACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAgg                                                                <  2:3752011/150‑1 (MQ=255)
                                                                                   aCTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAggg                                                               >  1:3000917/1‑150 (MQ=255)
                                                                                    cTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGt                                                              <  2:2854986/150‑1 (MQ=255)
                                                                                       tGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTaaa                                                           <  2:3143318/150‑1 (MQ=255)
                                                                                        ggTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTaaaa                                                          >  1:330286/1‑150 (MQ=255)
                                                                                          tgtAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAg                                                        <  1:1640103/150‑1 (MQ=255)
                                                                                             aaCGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGaaa                                                     >  1:231110/1‑150 (MQ=255)
                                                                                             aaCGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGaaa                                                     <  1:1930632/150‑1 (MQ=255)
                                                                                              aCGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAAc                                                    <  2:3556846/150‑1 (MQ=255)
                                                                                                   tCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCATGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCa                                               >  1:3065736/1‑150 (MQ=255)
                                                                                                      cgtcgtCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACg                                            <  1:2813798/150‑1 (MQ=255)
                                                                                                      cgtcgtCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACg                                            >  1:2459267/1‑150 (MQ=255)
                                                                                                      cgtcgtCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACg                                            <  1:132876/150‑1 (MQ=255)
                                                                                                       ttagtcCCCACAGCGTCATTGTCAACGACGTACCAACAGATCCTTACAATGGACGAAAAAAATTACTCATACAAAATGCATTGGAAAATAAATTAGATGGCAAGCCTGAAAACTAAATCGTTTATAAGGGTAAAAAGAAACAGGCAACgg                                           >  2:796030/4‑150 (MQ=255)
                                                                                                       gtcgtcCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACgg                                           >  2:2512157/1‑150 (MQ=255)
                                                                                                        tcgtcCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGc                                          <  2:3954625/150‑1 (MQ=255)
                                                                                                          gtcCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGa                                        <  1:2939753/150‑1 (MQ=255)
                                                                                                          gtcCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGa                                        >  1:2134147/1‑150 (MQ=255)
                                                                                                          gtcCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGa                                        <  1:27618/150‑1 (MQ=255)
                                                                                                            ccccACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAAt                                      >  2:510964/1‑150 (MQ=255)
                                                                                                                acaGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCt                                  <  2:1056606/150‑1 (MQ=255)
                                                                                                                  aGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGa                                >  1:2723028/1‑150 (MQ=255)
                                                                                                                   gCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGaa                               <  1:650351/150‑1 (MQ=255)
                                                                                                                     gTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGaaaa                             <  2:1201554/150‑1 (MQ=255)
                                                                                                                      tCATTGTCAACGACGTACCAACTGATCCTTACAAGTTACGAAAAAAATTACACATACTCGAGGCATTGTCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCACTGaaaaa                            >  1:2622493/1‑150 (MQ=255)
                                                                                                                       aaTTGTCAACGACGTACCAAATGATCCTTACAAGGTACGAAAAAAATTACTAATACAATATGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACTAAATCGTTGTTAATGGTAAAAAGAAACAGGCAAAAGCGAATCCCTGaaaaaa                           >  2:209086/2‑150 (MQ=255)
                                                                                                                        aTTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAAc                          >  2:1601180/1‑150 (MQ=255)
                                                                                                                        aTTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAAc                          <  2:622496/150‑1 (MQ=255)
                                                                                                                         ttGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACt                         <  1:818457/150‑1 (MQ=255)
                                                                                                                           gTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGc                       <  2:1442928/150‑1 (MQ=255)
                                                                                                                             cAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCgg                     >  2:3539709/1‑150 (MQ=255)
                                                                                                                              aaCGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGc                    <  1:1219554/150‑1 (MQ=255)
                                                                                                                                     tACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatga             <  2:1789672/150‑1 (MQ=255)
                                                                                                                                     tACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatga             <  2:741970/150‑1 (MQ=255)
                                                                                                                                         aaCTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGcc         >  1:2136229/1‑150 (MQ=255)
                                                                                                                                         aaCTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGcc         >  1:3767167/1‑150 (MQ=255)
                                                                                                                                          aCTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACAGCGGCAAAATGATGCCt        >  2:4563612/1‑150 (MQ=255)
                                                                                                                                          aCTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCATGAAAAAACTGCGGCAAAATGATGCCt        >  2:4347422/1‑150 (MQ=255)
                                                                                                                                            tGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGt      <  1:3971829/150‑1 (MQ=255)
                                                                                                                                            tGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGt      >  2:3726555/1‑150 (MQ=255)
                                                                                                                                                ccTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTaaaa  <  2:140525/150‑1 (MQ=255)
                                                                                                                                                ccTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTaaaa  <  2:3027648/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAA  >  CP009273/2474271‑2474564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  CP009273/2474269‑2474567
                                                                                                                                                     |                                                                                                                                                     
CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTT                                                                                                                                                       <  SRR19973072.3467596/150‑1 (MQ=60)
 TGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTA                                                                                                                                                      <  SRR19973072.2357352/150‑1 (MQ=60)
 TGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTA                                                                                                                                                      <  SRR19973072.2357336/150‑1 (MQ=60)
  GAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTAC                                                                                                                                                     >  SRR19973072.2237964/1‑150 (MQ=60)
   AAAGCGAACCATAATATGTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACA                                                                                                                                                    <  SRR19973072.2320813/150‑1 (MQ=60)
    AAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAA                                                                                                                                                   <  SRR19973072.1547353/150‑1 (MQ=60)
    AAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAA                                                                                                                                                   <  SRR19973072.1853111/150‑1 (MQ=60)
    AAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAA                                                                                                                                                   >  SRR19973072.1202080/1‑150 (MQ=60)
      GCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGG                                                                                                                                                 <  SRR19973072.1806875/150‑1 (MQ=60)
       CGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGT                                                                                                                                                <  SRR19973072.1587081/150‑1 (MQ=60)
        GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                                                                                               >  SRR19973072.3393441/1‑150 (MQ=60)
         AACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTAC                                                                                                                                              <  SRR19973072.2260113/150‑1 (MQ=60)
          ACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG                                                                                                                                             <  SRR19973072.2934449/150‑1 (MQ=60)
            CATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAA                                                                                                                                           >  SRR19973072.1331430/1‑150 (MQ=60)
              TAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAA                                                                                                                                         >  SRR19973072.4532990/1‑150 (MQ=60)
               AATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAA                                                                                                                                        >  SRR19973072.4513633/1‑150 (MQ=60)
                ATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAA                                                                                                                                       >  SRR19973072.3780813/1‑150 (MQ=60)
                ATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAA                                                                                                                                       >  SRR19973072.3799549/1‑150 (MQ=60)
                ATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCACCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAA                                                                                                                                       <  SRR19973072.2557397/150‑1 (MQ=60)
                 TATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAA                                                                                                                                      >  SRR19973072.4637630/1‑150 (MQ=60)
                   TTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATT                                                                                                                                    <  SRR19973072.3089192/150‑1 (MQ=60)
                   TTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATT                                                                                                                                    <  SRR19973072.3450180/150‑1 (MQ=60)
                    TTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTA                                                                                                                                   <  SRR19973072.339798/150‑1 (MQ=60)
                     TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                                                                                  <  SRR19973072.2428270/150‑1 (MQ=60)
                     TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                                                                                  >  SRR19973072.2345887/1‑150 (MQ=60)
                     TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                                                                                  >  SRR19973072.46218/1‑150 (MQ=60)
                     TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                                                                                  >  SRR19973072.46214/1‑150 (MQ=60)
                     TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                                                                                  >  SRR19973072.2346442/1‑150 (MQ=60)
                     TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                                                                                  >  SRR19973072.3486341/1‑150 (MQ=60)
                     TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                                                                                  <  SRR19973072.4215321/150‑1 (MQ=60)
                     TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                                                                                  >  SRR19973072.2847657/1‑150 (MQ=60)
                        CCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCA                                                                                                                               <  SRR19973072.2264693/150‑1 (MQ=60)
                         CATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCAT                                                                                                                              >  SRR19973072.1418624/1‑150 (MQ=60)
                         CATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCAT                                                                                                                              >  SRR19973072.1058875/1‑150 (MQ=60)
                          ATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATA                                                                                                                             >  SRR19973072.697273/1‑150 (MQ=60)
                            AATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACT                                                                                                                           <  SRR19973072.4450621/150‑1 (MQ=60)
                               TTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGA                                                                                                                        >  SRR19973072.2064813/1‑150 (MQ=60)
                                   GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCA                                                                                                                    <  SRR19973072.834309/150‑1 (MQ=60)
                                     CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATT                                                                                                                  >  SRR19973072.59210/1‑150 (MQ=60)
                                                 CACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATT                                                                                                      <  SRR19973072.2078221/150‑1 (MQ=60)
                                                   CGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTAT                                                                                                    >  SRR19973072.4496797/1‑150 (MQ=60)
                                                      GGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTG                                                                                                 >  SRR19973072.4648174/1‑150 (MQ=60)
                                                      GGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTG                                                                                                 >  SRR19973072.1467571/1‑150 (MQ=60)
                                                        TTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGC                                                                                               <  SRR19973072.4300558/150‑1 (MQ=60)
                                                        TTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGC                                                                                               <  SRR19973072.4300527/150‑1 (MQ=60)
                                                         TAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                                                                                              <  SRR19973072.113413/150‑1 (MQ=60)
                                                         TAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                                                                                              <  SRR19973072.113529/150‑1 (MQ=60)
                                                         TAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                                                                                              >  SRR19973072.3907824/1‑150 (MQ=60)
                                                          AAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAA                                                                                             <  SRR19973072.1028354/150‑1 (MQ=60)
                                                           AATTTATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAG                                                                                            >  SRR19973072.3062600/1‑150 (MQ=60)
                                                            ATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGC                                                                                           >  SRR19973072.3539543/1‑150 (MQ=60)
                                                             TTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCC                                                                                          <  SRR19973072.4320101/150‑1 (MQ=60)
                                                             TTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCC                                                                                          <  SRR19973072.4320032/150‑1 (MQ=60)
                                                                 tagctgggTGCTGTCAACTGACTATGGTGTTAAGCTTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCCACTGATCCTTACAACGTAAGTAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAA                                                                                      <  SRR19973072.15044/142‑1 (MQ=60)
                                                                 TCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAA                                                                                      <  SRR19973072.2094172/150‑1 (MQ=60)
                                                                  CGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAA                                                                                     >  SRR19973072.3507927/1‑150 (MQ=60)
                                                                     TGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACG                                                                                  >  SRR19973072.3482009/1‑150 (MQ=60)
                                                                         TGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAGTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAAT                                                                              >  SRR19973072.4107741/1‑150 (MQ=60)
                                                                           CTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCG                                                                            <  SRR19973072.2372324/150‑1 (MQ=60)
                                                                             GTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTT                                                                          >  SRR19973072.3857728/1‑150 (MQ=60)
                                                                                AACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT                                                                       >  SRR19973072.4452566/1‑150 (MQ=60)
                                                                                AACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT                                                                       >  SRR19973072.4452903/1‑150 (MQ=60)
                                                                                 ACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTA                                                                      >  SRR19973072.2868774/1‑150 (MQ=60)
                                                                                 ACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTA                                                                      >  SRR19973072.673604/1‑150 (MQ=60)
                                                                                 ACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTA                                                                      >  SRR19973072.3473561/1‑150 (MQ=60)
                                                                                    GACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG                                                                   <  SRR19973072.3362925/150‑1 (MQ=60)
                                                                                     ACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGG                                                                  >  SRR19973072.3007306/1‑150 (MQ=60)
                                                                                      CTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGT                                                                 >  SRR19973072.3322600/1‑150 (MQ=60)
                                                                                          GGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAA                                                             >  SRR19973072.4415557/1‑150 (MQ=60)
                                                                                          GGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAA                                                             >  SRR19973072.331029/1‑150 (MQ=60)
                                                                                            TGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAG                                                           <  SRR19973072.2721156/150‑1 (MQ=60)
                                                                                            TGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAG                                                           <  SRR19973072.1643683/150‑1 (MQ=60)
                                                                                            TGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAG                                                           <  SRR19973072.1643517/150‑1 (MQ=60)
                                                                                             GTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGA                                                          >  SRR19973072.2408477/1‑150 (MQ=60)
                                                                                             GTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGA                                                          <  SRR19973072.811205/150‑1 (MQ=60)
                                                                                              TAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAA                                                         >  SRR19973072.2996660/1‑150 (MQ=60)
                                                                                              TAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAA                                                         >  SRR19973072.1679240/1‑150 (MQ=60)
                                                                                              TAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGGTGTTAAGGGTAAAAAGAA                                                         >  SRR19973072.2996669/1‑150 (MQ=60)
                                                                                               AACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAA                                                        >  SRR19973072.231681/1‑150 (MQ=60)
                                                                                               AACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAA                                                        <  SRR19973072.1934729/150‑1 (MQ=60)
                                                                                                     TCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCATGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCA                                                  >  SRR19973072.3072211/1‑150 (MQ=60)
                                                                                                        CGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACG                                               <  SRR19973072.133211/150‑1 (MQ=60)
                                                                                                        CGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACG                                               <  SRR19973072.2819825/150‑1 (MQ=60)
                                                                                                        CGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACG                                               >  SRR19973072.2464608/1‑150 (MQ=60)
                                                                                                         GTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGG                                              >  SRR19973072.563962/1‑150 (MQ=60)
                                                                                                         GTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGG                                              >  SRR19973072.1285168/1‑150 (MQ=60)
                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           <  SRR19973072.560485/150‑1 (MQ=60)
                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           <  SRR19973072.2946041/150‑1 (MQ=60)
                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           <  SRR19973072.27645/150‑1 (MQ=60)
                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           >  SRR19973072.2138798/1‑150 (MQ=60)
                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           >  SRR19973072.2139537/1‑150 (MQ=60)
                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           <  SRR19973072.27644/150‑1 (MQ=60)
                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           <  SRR19973072.27692/150‑1 (MQ=60)
                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           <  SRR19973072.3042619/150‑1 (MQ=60)
                                                                                                              CCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAAT                                         >  SRR19973072.643327/1‑150 (MQ=60)
                                                                                                              CCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAAT                                         >  SRR19973072.643348/1‑150 (MQ=60)
                                                                                                                  ACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCT                                     >  SRR19973072.1051616/1‑150 (MQ=60)
                                                                                                                    AGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGA                                   >  SRR19973072.2728837/1‑150 (MQ=60)
                                                                                                                     GCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAA                                  <  SRR19973072.653385/150‑1 (MQ=60)
                                                                                                                     GCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAA                                  <  SRR19973072.651823/150‑1 (MQ=60)
                                                                                                                       GTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAA                                <  SRR19973072.2783684/150‑1 (MQ=60)
                                                                                                                        TCATTGTCAACGACGTACCAACTGATCCTTACAAGTTACGAAAAAAATTACACATACTCGAGGCATTGTCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCACTGAAAAA                               >  SRR19973072.2628134/1‑150 (MQ=60)
                                                                                                                           TTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACT                            <  SRR19973072.4201405/150‑1 (MQ=60)
                                                                                                                           TTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACT                            <  SRR19973072.2563511/150‑1 (MQ=60)
                                                                                                                           TTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACT                            <  SRR19973072.1931859/150‑1 (MQ=60)
                                                                                                                           TTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACT                            <  SRR19973072.820215/150‑1 (MQ=60)
                                                                                                                             GTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGC                          >  SRR19973072.4639960/1‑150 (MQ=60)
                                                                                                                              TCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCG                         >  SRR19973072.2104565/1‑150 (MQ=60)
                                                                                                                                AACGACGTACCAACTGATCCTTACACGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGC                       <  SRR19973072.1222160/150‑1 (MQ=60)
                                                                                                                                AACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGC                       <  SRR19973072.1222101/150‑1 (MQ=60)
                                                                                                                                AACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGC                       <  SRR19973072.2840675/150‑1 (MQ=60)
                                                                                                                                   GACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAA                    <  SRR19973072.2298470/150‑1 (MQ=60)
                                                                                                                                     CGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAANAGAAACAGGNAACGGCGANTCCCTGAANAAACTGCGNCACAAT                  >  SRR19973072.2122830/1‑150 (MQ=60)
                                                                                                                                      GTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATG                 <  SRR19973072.3643631/150‑1 (MQ=60)
                                                                                                                                        ACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGAT               >  SRR19973072.2338766/1‑150 (MQ=60)
                                                                                                                                           AACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCC            >  SRR19973072.2140886/1‑150 (MQ=60)
                                                                                                                                           AACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCC            >  SRR19973072.3775073/1‑150 (MQ=60)
                                                                                                                                             CTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTG          <  SRR19973072.1903556/150‑1 (MQ=60)
                                                                                                                                             CTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTG          <  SRR19973072.4262076/150‑1 (MQ=60)
                                                                                                                                              TGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGT         <  SRR19973072.3670474/150‑1 (MQ=60)
                                                                                                                                              TGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGT         <  SRR19973072.3980136/150‑1 (MQ=60)
                                                                                                                                                     TACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  SRR19973072.218491/1‑150 (MQ=60)
                                                                                                                                                     TACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  SRR19973072.4347482/1‑150 (MQ=60)
                                                                                                                                                     TACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  SRR19973072.4347410/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  CP009273/2474269‑2474567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑