Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F142 I0 R1	859	274.6	8723680	96.4%	8409627	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,474,418	G→T	89.9%	S413* (TCA→TAA)	emrY ←	putative multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,474,418	0	G	T	89.9%	672.9 / 68.4	237	S413* (TCA→TAA)	emrY	putative multidrug efflux system
Reads supporting (aligned to +/- strand): ref base G (16/8); new base T (95/118); total (111/126)
Fisher's exact test for biased strand distribution p-value = 5.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

Only 100 of 239 total aligned reads displayed.

CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  CP009273/2474269‑2474567

cTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTg                                                                                                                                                       <  1:2195395/150‑1 (MQ=255)

    aaGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACaa                                                                                                                                                   >  1:1264865/1‑150 (MQ=255)

     aGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAg                                                                                                                                                  >  2:611238/1‑150 (MQ=255)

      gCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAgg                                                                                                                                                 >  2:77551/1‑150 (MQ=255)

       cGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGt                                                                                                                                                >  1:4061336/1‑150 (MQ=255)

        gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTa                                                                                                                                               >  1:1144113/1‑150 (MQ=255)

        gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTa                                                                                                                                               <  2:2142999/150‑1 (MQ=255)

        gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTa                                                                                                                                               >  2:2154808/1‑150 (MQ=255)

          aCCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACg                                                                                                                                             <  1:3553498/150‑1 (MQ=255)

           ccATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGa                                                                                                                                            >  1:1005665/1‑150 (MQ=255)

               aataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaa                                                                                                                                        <  1:3816384/150‑1 (MQ=255)

                atatTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaaa                                                                                                                                       >  2:4115030/1‑150 (MQ=255)

                 tatTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaaaa                                                                                                                                      >  1:3529328/1‑150 (MQ=255)

                  atTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAt                                                                                                                                     <  1:2067623/150‑1 (MQ=255)

                    ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTAc                                                                                                                                              <  2:2342916/139‑1 (MQ=255)

                    ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTa                                                                                                                                   >  2:105496/1‑150 (MQ=255)

                    ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTa                                                                                                                                   >  2:4182784/1‑150 (MQ=255)

                    ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTa                                                                                                                                   >  1:2284874/1‑150 (MQ=255)

                     tATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAc                                                                                                                                  <  2:2297977/150‑1 (MQ=255)

                     tATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAc                                                                                                                                  >  1:173011/1‑150 (MQ=255)

                       tCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTc                                                                                                                                <  1:538628/150‑1 (MQ=255)

                       tCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTc                                                                                                                                >  1:916457/1‑150 (MQ=255)

                          aTAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATa                                                                                                                             >  2:4041248/1‑150 (MQ=255)

                           tAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATAc                                                                                                                            >  1:3783589/1‑150 (MQ=255)

                             aTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTc                                                                                                                          >  2:1493096/1‑150 (MQ=255)

                             aTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTc                                                                                                                          <  2:2203373/150‑1 (MQ=255)

                             aTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTc                                                                                                                          <  2:2203347/150‑1 (MQ=255)

                              tttGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCg                                                                                                                         >  1:3834950/1‑150 (MQ=255)

                              tttGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCg                                                                                                                         <  1:2431158/150‑1 (MQ=255)

                                  tgACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGc                                                                                                                     >  1:997310/1‑150 (MQ=255)

                                   gACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCa                                                                                                                    <  1:695424/150‑1 (MQ=255)

                                    aCGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAt                                                                                                                   >  2:2488258/1‑150 (MQ=255)

                                     cGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAtt                                                                                                                  >  1:2480472/1‑150 (MQ=255)

                                       aTGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTgg                                                                                                                <  2:94158/150‑1 (MQ=255)

                                         gAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCa                                                                                                              >  1:325043/1‑150 (MQ=255)

                                          aaTTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCaa                                                                                                             >  2:3074333/1‑150 (MQ=255)

                                            ttAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAt                                                                                                           >  2:1178487/1‑150 (MQ=255)

                                            ttAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAt                                                                                                           >  2:3376274/1‑150 (MQ=255)

                                            ttAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAATTACTCATACTCGAGGCATTGGCAAAtt                                                                                                          >  1:1357959/1‑150 (MQ=255)

                                               aaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATtta                                                                                                        >  2:3363602/1‑150 (MQ=255)

                                                 cacGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatt                                                                                                      >  2:2904004/1‑150 (MQ=255)

                                                       gTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTgg                                                                                                >  1:885417/1‑150 (MQ=255)

                                                          aaaTTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCaa                                                                                             >  1:3927738/1‑150 (MQ=255)

                                                           aaTTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAg                                                                                            >  2:3949352/1‑150 (MQ=255)

                                                             ttGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGcc                                                                                          >  2:472939/1‑150 (MQ=255)

                                                             ttGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGACATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGcc                                                                                          <  2:2590552/150‑1 (MQ=255)

                                                              tGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCt                                                                                         <  2:188848/150‑1 (MQ=255)

                                                              tGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCt                                                                                         <  1:3365108/150‑1 (MQ=255)

                                                              tGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCt                                                                                         >  1:3199075/1‑150 (MQ=255)

                                                               gATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTg                                                                                        >  1:769506/1‑150 (MQ=255)

                                                                  cGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaaa                                                                                     >  1:4081318/1‑150 (MQ=255)

                                                                  cGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaaa                                                                                     <  2:4304974/150‑1 (MQ=255)

                                                                   gATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaaaa                                                                                    <  2:1852100/150‑1 (MQ=255)

                                                                   gATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaaaa                                                                                    >  2:825330/1‑150 (MQ=255)

                                                                    aTGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAc                                                                                   >  2:551771/1‑150 (MQ=255)

                                                                      ggTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGa                                                                                 <  1:3508114/150‑1 (MQ=255)

                                                                       gTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaa                                                                                >  1:668259/1‑150 (MQ=255)

                                                                       gTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaa                                                                                >  1:3069628/1‑150 (MQ=255)

                                                                         tGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAAt                                                                              <  1:2617105/150‑1 (MQ=255)

                                                                          gCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATc                                                                             <  1:131004/150‑1 (MQ=255)

                                                                           cTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCg                                                                            <  1:3021498/150‑1 (MQ=255)

                                                                           cTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCg                                                                            <  1:1255764/150‑1 (MQ=255)

                                                                            tGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgt                                                                           <  1:291243/150‑1 (MQ=255)

                                                                                aaCTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgtt                                                                       >  2:3764571/1‑150 (MQ=255)

                                                                                aaCTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgtt                                                                       <  1:457038/150‑1 (MQ=255)

                                                                                      cTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGt                                                                 >  1:1714823/1‑150 (MQ=255)

                                                                                        agggTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTaa                                                               <  2:3992518/148‑1 (MQ=255)

                                                                                        aTGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTaa                                                               <  1:419355/150‑1 (MQ=255)

                                                                                           gtgtAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTaaaaa                                                            <  2:1264865/150‑1 (MQ=255)

                                                                                            tgtAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAg                                                           <  2:3156744/150‑1 (MQ=255)

                                                                                             gtAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTCAAAAGa                                                          >  1:4051359/1‑150 (MQ=255)

                                                                                             gtAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGa                                                          <  1:1216731/150‑1 (MQ=255)

                                                                                                   aTTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAgg                                                    >  2:1991598/1‑150 (MQ=255)

                                                                                                   aTTCGCGTCGTCCCCACAGCGTCAGTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATGGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTCAAAAGAAACAgg                                                    >  2:2935491/1‑150 (MQ=255)

                                                                                                    ttCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGc                                                   >  1:3751332/1‑150 (MQ=255)

                                                                                                     tCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCa                                                  >  2:1843344/1‑150 (MQ=255)

                                                                                                      cgcgTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCaa                                                 <  1:2260889/150‑1 (MQ=255)

                                                                                                      cgcgTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCaa                                                 >  1:1765096/1‑150 (MQ=255)

                                                                                                      cgcgTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCaa                                                 <  1:2155863/150‑1 (MQ=255)

                                                                                                      cgcgTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCaa                                                 >  1:2384922/1‑150 (MQ=255)

                                                                                                      cgcgTCGTCCACACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCATATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCaa                                                 <  2:963783/150‑1 (MQ=255)

                                                                                                        cgtcgtCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACg                                               <  2:2803200/150‑1 (MQ=255)

                                                                                                           cgtcCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCg                                            <  2:4267036/150‑1 (MQ=255)

                                                                                                           cgtcCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCg                                            <  2:125895/150‑1 (MQ=255)

                                                                                                            gtcCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTCAGGGTAAAAAGAAACAGGCAACGGCGa                                           >  1:2602456/1‑150 (MQ=255)

                                                                                                              ccccACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAAt                                         >  2:1790725/1‑150 (MQ=255)

                                                                                                              ccccACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAAt                                         >  2:518916/1‑150 (MQ=255)

                                                                                                               cccACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATc                                        >  1:1968505/1‑150 (MQ=255)

                                                                                                                  acaGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCt                                     <  1:553966/150‑1 (MQ=255)

                                                                                                                    aGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGa                                   >  2:3384294/1‑150 (MQ=255)

                                                                                                                    aGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGa                                   >  2:2262519/1‑150 (MQ=255)

                                                                                                                           ttGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACt                            <  2:3751332/150‑1 (MQ=255)

                                                                                                                           ttGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACt                            <  1:3353190/150‑1 (MQ=255)

                                                                                                                           ttGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACt                            <  2:1690764/150‑1 (MQ=255)

                                                                                                                             gTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGc                          <  2:3836635/150‑1 (MQ=255)

                                                                                                                                     cgTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAat                  <  2:3515312/150‑1 (MQ=255)

                                                                                                                                      gTACCAACTGATCACTAAAAGCTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGTTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatg                 <  2:3799441/150‑1 (MQ=255)

                                                                                                                                        aCCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatgat               >  1:222114/1‑150 (MQ=255)

                                                                                                                                          cAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGc             <  1:2736829/150‑1 (MQ=255)

                                                                                                                                            aCTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCt           <  1:1541254/150‑1 (MQ=255)

CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  CP009273/2474269‑2474567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  CP009273/2474269‑2474567

CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTG                                                                                                                                                        <  SRR19973079.2206689/150‑1 (MQ=60)

    AAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAA                                                                                                                                                    >  SRR19973079.1272103/1‑150 (MQ=60)

    AAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAA                                                                                                                                                    >  SRR19973079.1271782/1‑150 (MQ=60)

       CGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGT                                                                                                                                                 >  SRR19973079.4081906/1‑150 (MQ=60)

       CGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGT                                                                                                                                                 >  SRR19973079.1552519/1‑150 (MQ=60)

       CGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGT                                                                                                                                                 >  SRR19973079.4082117/1‑150 (MQ=60)

        GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTTTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                                                                                                <  SRR19973079.2187857/150‑1 (MQ=60)

        GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                                                                                                >  SRR19973079.1150673/1‑150 (MQ=60)

         AACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTAC                                                                                                                                               >  SRR19973079.4272898/1‑150 (MQ=60)

          ACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG                                                                                                                                              <  SRR19973079.3571674/150‑1 (MQ=60)

           CCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑A                                                                                                                                            <  SRR19973079.103419/150‑1 (MQ=60)

           CCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑A                                                                                                                                            >  SRR19973079.1011333/1‑150 (MQ=60)

               AATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAA                                                                                                                                        <  SRR19973079.3836281/150‑1 (MQ=60)

               AATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAA                                                                                                                                        <  SRR19973079.3910363/150‑1 (MQ=60)

               AATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAA                                                                                                                                        <  SRR19973079.3835917/150‑1 (MQ=60)

                ATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAA                                                                                                                                       >  SRR19973079.195147/1‑150 (MQ=60)

                 TATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAA                                                                                                                                      >  SRR19973079.3547432/1‑150 (MQ=60)

                  ATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAAT                                                                                                                                     <  SRR19973079.2078446/150‑1 (MQ=60)

                    TTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACagatcggaaga                                                                                                                                    >  SRR19973079.2355080/1‑139 (MQ=60)

                    TTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTA                                                                                                                                   <  SRR19973079.1680928/150‑1 (MQ=60)

                    TTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTA                                                                                                                                   >  SRR19973079.2296731/1‑150 (MQ=60)

                     TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTAC                                                                                                                                  >  SRR19973079.174315/1‑150 (MQ=60)

                       TCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTC                                                                                                                                <  SRR19973079.3778403/150‑1 (MQ=60)

                       TCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTC                                                                                                                                <  SRR19973079.541859/150‑1 (MQ=60)

                       TCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTC                                                                                                                                >  SRR19973079.921752/1‑150 (MQ=60)

                       TCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTC                                                                                                                                >  SRR19973079.920760/1‑150 (MQ=60)

                         CATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCAT                                                                                                                              <  SRR19973079.556304/150‑1 (MQ=60)

                         CATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCAT                                                                                                                              <  SRR19973079.3821065/150‑1 (MQ=60)

                         CATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCAT                                                                                                                              <  SRR19973079.1305062/150‑1 (MQ=60)

                          ATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATA                                                                                                                             <  SRR19973079.1770532/150‑1 (MQ=60)

                           TAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATAC                                                                                                                            >  SRR19973079.3802965/1‑150 (MQ=60)

                            AATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACT                                                                                                                           >  SRR19973079.3549776/1‑150 (MQ=60)

                            AATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACT                                                                                                                           >  SRR19973079.3527121/1‑150 (MQ=60)

                             ATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTC                                                                                                                          <  SRR19973079.4054640/150‑1 (MQ=60)

                              TTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCG                                                                                                                         <  SRR19973079.2443670/150‑1 (MQ=60)

                              TTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCG                                                                                                                         <  SRR19973079.4075793/150‑1 (MQ=60)

                              TTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCG                                                                                                                         >  SRR19973079.3854539/1‑150 (MQ=60)

                               TTGTGACGATGAATTTAAAACGGGGTTTAATTGATAGTTGGTTGCTGTCAAATGACTATTGTGTAAAGATTCGCGTCGTCCCCACATCGTAATTGTCAACGTCGTAACAACTGATCCTTACAAGGTTCGAAAAAAAATTACTCATACTCG                                                                                                                         <  SRR19973079.359552/150‑1 (MQ=60)

                                TGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAG                                                                                                                       >  SRR19973079.673498/1‑150 (MQ=60)

                                TGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAG                                                                                                                       >  SRR19973079.2240624/1‑150 (MQ=60)

                                TGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAG                                                                                                                       <  SRR19973079.272821/150‑1 (MQ=60)

                                TGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAG                                                                                                                       <  SRR19973079.1171135/150‑1 (MQ=60)

                                  TGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGC                                                                                                                     >  SRR19973079.1002948/1‑150 (MQ=60)

                                   GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCA                                                                                                                    <  SRR19973079.2880532/150‑1 (MQ=60)

                                   GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCA                                                                                                                    >  SRR19973079.3317119/1‑150 (MQ=60)

                                   GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCA                                                                                                                    >  SRR19973079.3317202/1‑150 (MQ=60)

                                   GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCA                                                                                                                    <  SRR19973079.699173/150‑1 (MQ=60)

                                   GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCA                                                                                                                    >  SRR19973079.519216/1‑150 (MQ=60)

                                   GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCA                                                                                                                    <  SRR19973079.699198/150‑1 (MQ=60)

                                   GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCA                                                                                                                    <  SRR19973079.199570/150‑1 (MQ=60)

                                     CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATT                                                                                                                  >  SRR19973079.2492356/1‑150 (MQ=60)

                                     CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATT                                                                                                                  >  SRR19973079.2493118/1‑150 (MQ=60)

                                         GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCA                                                                                                              >  SRR19973079.326888/1‑150 (MQ=60)

                                          AATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAA                                                                                                             >  SRR19973079.3627997/1‑150 (MQ=60)

                                           ATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAA                                                                                                            >  SRR19973079.2511454/1‑150 (MQ=60)

                                            TTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑‑AAAAAATTACTCATACTCGAGGCATTGGCAAATT                                                                                                          >  SRR19973079.1365524/1‑150 (MQ=60)

                                              AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                                                                                         >  SRR19973079.1346952/1‑150 (MQ=60)

                                                       GTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGG                                                                                                >  SRR19973079.890550/1‑150 (MQ=60)

                                                       GTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGG                                                                                                <  SRR19973079.4213453/150‑1 (MQ=60)

                                                       GTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGG                                                                                                <  SRR19973079.4213422/150‑1 (MQ=60)

                                                          AAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAA                                                                                             >  SRR19973079.3947603/1‑150 (MQ=60)

                                                              TGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                                                                                         <  SRR19973079.3384018/150‑1 (MQ=60)

                                                              TGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                                                                                         <  SRR19973079.3382426/150‑1 (MQ=60)

                                                              TGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                                                                                         <  SRR19973079.3382276/150‑1 (MQ=60)

                                                              TGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                                                                                         >  SRR19973079.3214866/1‑150 (MQ=60)

                                                              TGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                                                                                         >  SRR19973079.3215401/1‑150 (MQ=60)

                                                               GATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTG                                                                                        >  SRR19973079.470701/1‑150 (MQ=60)

                                                               GATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTG                                                                                        >  SRR19973079.773796/1‑150 (MQ=60)

                                                                 TCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAA                                                                                      >  SRR19973079.2122293/1‑150 (MQ=60)

                                                                  CGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAA                                                                                     >  SRR19973079.4102000/1‑150 (MQ=60)

                                                                      GGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA                                                                                 <  SRR19973079.3078160/150‑1 (MQ=60)

                                                                      GGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA                                                                                 >  SRR19973079.3814211/1‑150 (MQ=60)

                                                                      GGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA                                                                                 <  SRR19973079.3526154/150‑1 (MQ=60)

                                                                       GTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAA                                                                                >  SRR19973079.3246553/1‑150 (MQ=60)

                                                                       GTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAA                                                                                >  SRR19973079.3085520/1‑150 (MQ=60)

                                                                       GTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAA                                                                                >  SRR19973079.3085501/1‑150 (MQ=60)

                                                                       GTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAA                                                                                >  SRR19973079.671977/1‑150 (MQ=60)

                                                                        TTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAA                                                                               >  SRR19973079.22474/1‑150 (MQ=60)

                                                                        TTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAA                                                                               >  SRR19973079.35138/1‑150 (MQ=60)

                                                                         TGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAAT                                                                              <  SRR19973079.2630599/150‑1 (MQ=60)

                                                                          GCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATC                                                                             <  SRR19973079.132071/150‑1 (MQ=60)

                                                                           CTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCG                                                                            <  SRR19973079.3037118/150‑1 (MQ=60)

                                                                           CTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCG                                                                            <  SRR19973079.2338323/150‑1 (MQ=60)

                                                                           CTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCG                                                                            <  SRR19973079.1356033/150‑1 (MQ=60)

                                                                           CTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCG                                                                            <  SRR19973079.1262957/150‑1 (MQ=60)

                                                                            TGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGT                                                                           <  SRR19973079.293154/150‑1 (MQ=60)

                                                                            TGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGT                                                                           <  SRR19973079.293002/150‑1 (MQ=60)

                                                                                AACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT                                                                       <  SRR19973079.459790/150‑1 (MQ=60)

                                                                                      CTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGT                                                                 >  SRR19973079.1723980/1‑150 (MQ=60)

                                                                                       TATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAAataaagaaacaggtggcaatggccaatttattaaaatgccagcccttaaaacgaaaactttgttagggta                                                                >  SRR19973079.1484884/1‑80 (MQ=60)

                                                                                        ATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAA                                                               <  SRR19973079.421824/150‑1 (MQ=60)

                                                                                         TGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTATGGGTATA                                                              >  SRR19973079.4016959/1‑150 (MQ=60)

                                                                                          GGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAA                                                             >  SRR19973079.2281561/1‑150 (MQ=60)

                                                                                             GTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTCAAAAGA                                                          >  SRR19973079.4071859/1‑150 (MQ=60)

                                                                                             GTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGA                                                          <  SRR19973079.1224066/150‑1 (MQ=60)

                                                                                             GTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGA                                                          <  SRR19973079.1223722/150‑1 (MQ=60)

                                                                                             GTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGA                                                          <  SRR19973079.1223743/150‑1 (MQ=60)

                                                                                                   ATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGG                                                    >  SRR19973079.1799762/1‑150 (MQ=60)

                                                                                                    TTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGC                                                   >  SRR19973079.3770549/1‑150 (MQ=60)

                                                                                                      CGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAA                                                 >  SRR19973079.2907343/1‑150 (MQ=60)

                                                                                                      CGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAA                                                 <  SRR19973079.2272614/150‑1 (MQ=60)

                                                                                                      CGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAA                                                 >  SRR19973079.1774383/1‑150 (MQ=60)

                                                                                                      CGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAA                                                 <  SRR19973079.2166921/150‑1 (MQ=60)

                                                                                                      CGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCAGACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAA                                                 <  SRR19973079.2166770/150‑1 (MQ=60)

                                                                                                      CGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAA                                                 >  SRR19973079.2397264/1‑150 (MQ=60)

                                                                                                      CGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAA                                                 >  SRR19973079.2397271/1‑150 (MQ=60)

                                                                                                      CGCGACGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAA                                                 <  SRR19973079.2573067/150‑1 (MQ=60)

                                                                                                         GTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGG                                              >  SRR19973079.968026/1‑150 (MQ=60)

                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTCAGGGTAAAAAGAAACAGGCAACGGCGA                                           >  SRR19973079.2615840/1‑150 (MQ=60)

                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           >  SRR19973079.2615842/1‑150 (MQ=60)

                                                                                                            GTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGA                                           >  SRR19973079.1738939/1‑150 (MQ=60)

                                                                                                              CCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAAT                                         <  SRR19973079.712037/150‑1 (MQ=60)

                                                                                                               CCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGTTACT‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTTTTATCTGGCAAGCCTTAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATC                                        <  SRR19973079.2206944/150‑1 (MQ=60)

                                                                                                               CCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATC                                        >  SRR19973079.1978972/1‑150 (MQ=60)

                                                                                                                  ACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAATTTACTCAGACTCGAGGCATTGGCAAATTTTTTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCT                                     <  SRR19973079.813723/150‑1 (MQ=60)

                                                                                                                  ACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCT                                     <  SRR19973079.557268/150‑1 (MQ=60)

                                                                                                                  ACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCT                                     <  SRR19973079.557389/150‑1 (MQ=60)

                                                                                                                     GCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAA                                  <  SRR19973079.795244/150‑1 (MQ=60)

                                                                                                                           TTGTCAACGACGTACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACT                            <  SRR19973079.3370409/150‑1 (MQ=60)

                                                                                                                                        ACCAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGAT               >  SRR19973079.223645/1‑150 (MQ=60)

                                                                                                                                          CAACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGC             <  SRR19973079.2750970/150‑1 (MQ=60)

                                                                                                                                            ACTGATCCTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCT           <  SRR19973079.1549760/150‑1 (MQ=60)

                                                                                                                                                   CTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAAT    <  SRR19973079.2433171/150‑1 (MQ=60)

                                                                                                                                                   CTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAAT    <  SRR19973079.2433181/150‑1 (MQ=60)

                                                                                                                                                   CTTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAAT    >  SRR19973079.1813522/1‑150 (MQ=60)

                                                                                                                                                    TTACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATC   >  SRR19973079.2021879/1‑150 (MQ=60)

                                                                                                                                                     TACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  SRR19973079.360496/1‑150 (MQ=60)

                                                                                                                                                     TACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  <  SRR19973079.4251531/150‑1 (MQ=60)

CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACG‑AAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCA  >  CP009273/2474269‑2474567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`