Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,476,524 T→A 100% N99Y (AAT→TAT)  emrK ← EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,476,5240TA95.1% 623.4 / 18.2 202N99Y (AAT→TAT) emrKEmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base A (107/85);  total (112/90)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

AACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGAC  >  CP009273/2476378‑2476671
                                                                                                                                                  |                                                                                                                                                   
aaCCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTAttt                                                                                                                                                    <  2:1556397/148‑1 (MQ=255)
aaCCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTAttt                                                                                                                                                    >  2:1104653/1‑148 (MQ=255)
  cccccTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTAtattt                                                                                                                                                  <  1:2597362/148‑1 (MQ=255)
  cccccTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTAtattt                                                                                                                                                  <  2:540556/148‑1 (MQ=255)
   ccccTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTa                                                                                                                                                 <  1:866945/148‑1 (MQ=255)
      cTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCt                                                                                                                                              <  1:1339855/148‑1 (MQ=255)
        gCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCttt                                                                                                                                            >  2:2631382/1‑148 (MQ=255)
        gCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTAGTTTGCCGAACAATATTTGCCAGATTATATTTAGCttt                                                                                                                                            <  1:701082/148‑1 (MQ=255)
         cTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTa                                                                                                                                           <  2:1252249/148‑1 (MQ=255)
                aaCGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTg                                                                                                                                    >  1:1743229/1‑148 (MQ=255)
                aaCGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTg                                                                                                                                    >  1:68091/1‑148 (MQ=255)
                     cACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATa                                                                                                                               <  1:545959/148‑1 (MQ=255)
                      aCTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAg                                                                                                                              <  2:680870/148‑1 (MQ=255)
                        tCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTg                                                                                                                            <  1:1442058/148‑1 (MQ=255)
                              gTTATAATCGTCTAAAGATTGTTGAAACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCa                                                                                                                      <  1:921727/148‑1 (MQ=255)
                               ttATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAg                                                                                                                     <  2:661468/148‑1 (MQ=255)
                               ttATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAg                                                                                                                     >  2:2648268/1‑148 (MQ=255)
                                tataATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGt                                                                                                                    >  1:1659358/1‑148 (MQ=255)
                                tataATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGt                                                                                                                    >  1:1486576/1‑148 (MQ=255)
                                tataATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGt                                                                                                                    >  1:3128457/1‑148 (MQ=255)
                                 ataATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGtt                                                                                                                   <  2:3213426/148‑1 (MQ=255)
                                     tcttctAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTAt                                                                                                               <  1:2721157/148‑1 (MQ=255)
                                      cttctAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATc                                                                                                              >  2:1140874/1‑148 (MQ=255)
                                            aaGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGa                                                                                                        >  2:2755861/1‑148 (MQ=255)
                                              gATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGaaa                                                                                                      <  1:35261/148‑1 (MQ=255)
                                              gATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGaaa                                                                                                      <  1:1044127/148‑1 (MQ=255)
                                              gATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGaaa                                                                                                      >  2:15377/1‑148 (MQ=255)
                                               aTTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAAc                                                                                                     >  2:3177891/1‑148 (MQ=255)
                                               aTTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAAc                                                                                                     <  2:745107/148‑1 (MQ=255)
                                                   ttgATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTaaa                                                                                                 <  1:1604520/148‑1 (MQ=255)
                                                       tACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGt                                                                                             >  1:2494238/1‑148 (MQ=255)
                                                           gAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCAcc                                                                                         >  1:192532/1‑148 (MQ=255)
                                                            aaTACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCt                                                                                        <  1:1355507/148‑1 (MQ=255)
                                                                   gCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGaa                                                                                 <  2:2678991/148‑1 (MQ=255)
                                                                   gCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGaa                                                                                 >  1:2710791/1‑148 (MQ=255)
                                                                        aGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAg                                                                            <  1:2130432/148‑1 (MQ=255)
                                                                        aGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAg                                                                            <  2:1644281/148‑1 (MQ=255)
                                                                        aGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAg                                                                            <  1:961466/148‑1 (MQ=255)
                                                                         gCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGt                                                                           >  2:2225907/1‑148 (MQ=255)
                                                                         gCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGt                                                                           >  1:1276387/1‑148 (MQ=255)
                                                                         gCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGt                                                                           <  2:2721556/148‑1 (MQ=255)
                                                                          cGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGtt                                                                          >  1:2476714/1‑148 (MQ=255)
                                                                            aCTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTct                                                                        >  2:2078221/1‑147 (MQ=255)
                                                                             cTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGt                                                                       >  2:940843/1‑148 (MQ=255)
                                                                              ttCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTa                                                                      >  1:1291041/1‑148 (MQ=255)
                                                                               tCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTAt                                                                     <  2:1392442/148‑1 (MQ=255)
                                                                                  gCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCtt                                                                  >  1:2672492/1‑148 (MQ=255)
                                                                                   cACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCttt                                                                 >  1:1962864/1‑148 (MQ=255)
                                                                                    aCTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTa                                                                >  1:2185948/1‑148 (MQ=255)
                                                                                      tGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAGACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATg                                                              <  2:2156000/148‑1 (MQ=255)
                                                                                      tGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATg                                                              >  1:2325033/1‑148 (MQ=255)
                                                                                      tGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTAGTATCTTTATg                                                              >  2:3080420/1‑148 (MQ=255)
                                                                                       gTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGa                                                             <  1:571974/148‑1 (MQ=255)
                                                                                       gTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGa                                                             <  1:2979863/148‑1 (MQ=255)
                                                                                          ttGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTa                                                          <  1:338929/148‑1 (MQ=255)
                                                                                          ttGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTa                                                          >  2:25411/1‑148 (MQ=255)
                                                                                           tGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTaa                                                         >  1:3276724/1‑148 (MQ=255)
                                                                                           tGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTaa                                                         >  1:2407179/1‑148 (MQ=255)
                                                                                             tttATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACg                                                       <  2:2444282/148‑1 (MQ=255)
                                                                                              ttATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGa                                                      <  2:3128457/148‑1 (MQ=255)
                                                                                                aTCCTTTAATTATTTTTTTTTCTTTTGCCTAACAATATTTGCAATATTATATTTAGATTTTTTGTGTGCGTTTGTGGAATCAGTTTTATCCAGTTAAACTAAAATGTCACATTGTCTAACGTAGTTCGTATCTTTATGATTAACGACa                                                    <  1:813590/148‑1 (MQ=255)
                                                                                                  ccTGTAAGTATATTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGt                                                  <  2:706647/148‑1 (MQ=255)
                                                                                                    tGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAGACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGa                                                >  2:74558/1‑148 (MQ=255)
                                                                                                    tGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGa                                                >  2:1041879/1‑148 (MQ=255)
                                                                                                     gTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGac                                               >  1:753486/1‑148 (MQ=255)
                                                                                                      tAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGaca                                              <  1:446807/148‑1 (MQ=255)
                                                                                                      tAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGaca                                              >  1:926180/1‑148 (MQ=255)
                                                                                                       aaGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGacac                                             >  2:3213389/1‑148 (MQ=255)
                                                                                                       aaGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGacac                                             >  1:394475/1‑148 (MQ=255)
                                                                                                       aaGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATAGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGacac                                             >  2:2740265/1‑148 (MQ=255)
                                                                                                         gTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTa                                           <  2:444729/148‑1 (MQ=255)
                                                                                                            taGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCt                                        >  1:2835726/1‑148 (MQ=255)
                                                                                                             aGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTg                                       >  2:83395/1‑148 (MQ=255)
                                                                                                                ttATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTgaga                                    <  1:1104615/148‑1 (MQ=255)
                                                                                                                  aTTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACt                                  >  2:1801279/1‑148 (MQ=255)
                                                                                                                  aTTCGTTTGCCGAACAATATTTGCCAGATTATATCTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACt                                  >  1:155965/1‑148 (MQ=255)
                                                                                                                    tCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTtg                                >  1:2468077/1‑148 (MQ=255)
                                                                                                                    tCGTCTGCCGAACAATATTGGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTtg                                <  1:2606592/148‑1 (MQ=255)
                                                                                                                        ttGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCa                            >  1:1712744/1‑148 (MQ=255)
                                                                                                                           ccGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGaa                         <  1:1473723/148‑1 (MQ=255)
                                                                                                                            cGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGaaa                        <  1:2271386/148‑1 (MQ=255)
                                                                                                                             gAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAt                       >  1:428329/1‑148 (MQ=255)
                                                                                                                              aaCAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAtt                      >  1:2270175/1‑148 (MQ=255)
                                                                                                                              aaCAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAtt                      >  2:2952973/1‑148 (MQ=255)
                                                                                                                                cAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTgg                    <  1:1829528/148‑1 (MQ=255)
                                                                                                                                cAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTgg                    >  1:3030678/1‑148 (MQ=255)
                                                                                                                                  atatTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGAt                  >  1:1173405/1‑148 (MQ=255)
                                                                                                                                  atatTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGAt                  >  1:1534071/1‑148 (MQ=255)
                                                                                                                                   tatTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATc                 >  1:2929344/1‑148 (MQ=255)
                                                                                                                                   tatTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATc                 >  1:632621/1‑148 (MQ=255)
                                                                                                                                    atTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCt                >  1:2559548/1‑148 (MQ=255)
                                                                                                                                     tttGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTg               >  1:2630451/1‑148 (MQ=255)
                                                                                                                                     tttGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTg               >  2:1666298/1‑148 (MQ=255)
                                                                                                                                      ttGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGc              <  1:2501758/148‑1 (MQ=255)
                                                                                                                                      ttGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGc              >  2:2800517/1‑148 (MQ=255)
                                                                                                                                      ttGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGc              >  2:2698874/1‑148 (MQ=255)
                                                                                                                                      ttGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGc              >  2:1894726/1‑148 (MQ=255)
                                                                                                                                      ttGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGc              <  2:192532/148‑1 (MQ=255)
                                                                                                                                          cAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTc          >  1:1372408/1‑148 (MQ=255)
                                                                                                                                                  ttttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGAc  >  2:1214524/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGAC  >  CP009273/2476378‑2476671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACAT  >  CP009273/2476375‑2476673
                                                                                                                                                     |                                                                                                                                                     
AATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATA                                                                                                                                                       >  SRR19973070.750766/1‑150 (MQ=60)
AATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATA                                                                                                                                                       <  SRR19973070.1564023/150‑1 (MQ=60)
  TAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATT                                                                                                                                                     <  SRR19973070.3190640/150‑1 (MQ=60)
   AACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTT                                                                                                                                                    <  SRR19973070.2121481/150‑1 (MQ=60)
   AACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTT                                                                                                                                                    <  SRR19973070.3034724/150‑1 (MQ=60)
     CCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAG                                                                                                                                                  <  SRR19973070.2614330/150‑1 (MQ=60)
     CCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAG                                                                                                                                                  <  SRR19973070.2614651/150‑1 (MQ=60)
      CCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGC                                                                                                                                                 <  SRR19973070.872999/150‑1 (MQ=60)
       CCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCT                                                                                                                                                >  SRR19973070.151829/1‑150 (MQ=60)
        CCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTT                                                                                                                                               <  SRR19973070.3164290/150‑1 (MQ=60)
         CTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTT                                                                                                                                              <  SRR19973070.1349088/150‑1 (MQ=60)
         CTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTT                                                                                                                                              <  SRR19973070.1349063/150‑1 (MQ=60)
           GCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTAT                                                                                                                                            <  SRR19973070.3323560/150‑1 (MQ=60)
           GCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTAGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTAT                                                                                                                                            <  SRR19973070.705844/150‑1 (MQ=60)
           GCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTAGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTAT                                                                                                                                            <  SRR19973070.706077/150‑1 (MQ=60)
             TTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTG                                                                                                                                          <  SRR19973070.893965/150‑1 (MQ=60)
               CGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAG                                                                                                                                        >  SRR19973070.1552265/1‑150 (MQ=60)
               CGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAG                                                                                                                                        <  SRR19973070.1482207/150‑1 (MQ=60)
                GCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGT                                                                                                                                       <  SRR19973070.183302/150‑1 (MQ=60)
                 CTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTG                                                                                                                                      >  SRR19973070.1755315/1‑150 (MQ=60)
                 CTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTG                                                                                                                                      >  SRR19973070.68845/1‑150 (MQ=60)
                      GGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATA                                                                                                                                 <  SRR19973070.2387999/150‑1 (MQ=60)
                        CACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGT                                                                                                                               <  SRR19973070.549595/150‑1 (MQ=60)
                         ACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTG                                                                                                                              >  SRR19973070.2435539/1‑150 (MQ=60)
                           TCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGC                                                                                                                            <  SRR19973070.1452062/150‑1 (MQ=60)
                                 GTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGT                                                                                                                      <  SRR19973070.930575/150‑1 (MQ=60)
                                 GTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGT                                                                                                                      >  SRR19973070.1670782/1‑150 (MQ=60)
                                 GTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGT                                                                                                                      >  SRR19973070.3148660/1‑150 (MQ=60)
                                 GTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGT                                                                                                                      >  SRR19973070.1496981/1‑150 (MQ=60)
                                 GTTATAATCGTCTAAAGATTGTTGAATCTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGT                                                                                                                      <  SRR19973070.928299/150‑1 (MQ=60)
                                  TTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTT                                                                                                                     >  SRR19973070.2170336/1‑150 (MQ=60)
                                   TATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTT                                                                                                                    <  SRR19973070.363463/150‑1 (MQ=60)
                                     TAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTA                                                                                                                  >  SRR19973070.232094/1‑150 (MQ=60)
                                        TCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCC                                                                                                               <  SRR19973070.2738719/150‑1 (MQ=60)
                                            CTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTG                                                                                                           >  SRR19973070.2525951/1‑150 (MQ=60)
                                                AGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAAC                                                                                                       >  SRR19973070.1592292/1‑150 (MQ=60)
                                                AGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAAC                                                                                                       <  SRR19973070.2985715/150‑1 (MQ=60)
                                                AGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAAC                                                                                                       >  SRR19973070.616377/1‑150 (MQ=60)
                                                AGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAAC                                                                                                       <  SRR19973070.1328904/150‑1 (MQ=60)
                                                 GATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACT                                                                                                      <  SRR19973070.1051491/150‑1 (MQ=60)
                                                 GATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACT                                                                                                      <  SRR19973070.35715/150‑1 (MQ=60)
                                                 GATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACT                                                                                                      <  SRR19973070.1051488/150‑1 (MQ=60)
                                                  ATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTA                                                                                                     >  SRR19973070.524597/1‑150 (MQ=60)
                                                   TTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAA                                                                                                    <  SRR19973070.2497185/150‑1 (MQ=60)
                                                      TTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAAT                                                                                                 <  SRR19973070.1615714/150‑1 (MQ=60)
                                                      TTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAAT                                                                                                 <  SRR19973070.2641082/150‑1 (MQ=60)
                                                       TGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATG                                                                                                <  SRR19973070.2349670/150‑1 (MQ=60)
                                                       TGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATG                                                                                                >  SRR19973070.2696380/1‑150 (MQ=60)
                                                        GATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGT                                                                                               >  SRR19973070.2510416/1‑150 (MQ=60)
                                                        GATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGT                                                                                               >  SRR19973070.2510488/1‑150 (MQ=60)
                                                           ACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCAC                                                                                            >  SRR19973070.268500/1‑150 (MQ=60)
                                                            CTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACC                                                                                           >  SRR19973070.194019/1‑150 (MQ=60)
                                                            CTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACC                                                                                           >  SRR19973070.193989/1‑150 (MQ=60)
                                                               AATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTG                                                                                        <  SRR19973070.1364774/150‑1 (MQ=60)
                                                                    GTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAA                                                                                   >  SRR19973070.2728922/1‑150 (MQ=60)
                                                                    GTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAA                                                                                   >  SRR19973070.2728314/1‑150 (MQ=60)
                                                                        TGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTA                                                                               >  SRR19973070.423711/1‑150 (MQ=60)
                                                                          AAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGT                                                                             >  SRR19973070.1285311/1‑150 (MQ=60)
                                                                           AGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTT                                                                            <  SRR19973070.968326/150‑1 (MQ=60)
                                                                           AGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTT                                                                            >  SRR19973070.2492845/1‑150 (MQ=60)
                                                                           AGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTT                                                                            <  SRR19973070.2144678/150‑1 (MQ=60)
                                                                           AGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATCTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTT                                                                            >  SRR19973070.2492782/1‑150 (MQ=60)
                                                                             CGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCG                                                                          <  SRR19973070.1809935/150‑1 (MQ=60)
                                                                               ACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTA                                                                        >  SRR19973070.755733/1‑150 (MQ=60)
                                                                               ACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTA                                                                        >  SRR19973070.1300039/1‑150 (MQ=60)
                                                                               ACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTA                                                                        >  SRR19973070.1300041/1‑150 (MQ=60)
                                                                                   CGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTT                                                                    >  SRR19973070.2689847/1‑150 (MQ=60)
                                                                                   CGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTT                                                                    <  SRR19973070.851033/150‑1 (MQ=60)
                                                                                    GGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTT                                                                   >  SRR19973070.1976103/1‑150 (MQ=60)
                                                                                    GGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTT                                                                   >  SRR19973070.1242267/1‑150 (MQ=60)
                                                                                     GCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTA                                                                  >  SRR19973070.2200398/1‑150 (MQ=60)
                                                                                       ACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATG                                                                >  SRR19973070.2340480/1‑150 (MQ=60)
                                                                                       ACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATG                                                                <  SRR19973070.2665504/150‑1 (MQ=60)
                                                                                        CTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGA                                                               <  SRR19973070.1939641/150‑1 (MQ=60)
                                                                                          GTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATT                                                             <  SRR19973070.575865/150‑1 (MQ=60)
                                                                                          GTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATT                                                             <  SRR19973070.3021250/150‑1 (MQ=60)
                                                                                          GTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATT                                                             <  SRR19973070.2998985/150‑1 (MQ=60)
                                                                                            ATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAA                                                           >  SRR19973070.3297781/1‑150 (MQ=60)
                                                                                            ATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAA                                                           >  SRR19973070.2423055/1‑150 (MQ=60)
                                                                                             TTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAAC                                                          <  SRR19973070.341400/150‑1 (MQ=60)
                                                                                             TTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAAC                                                          <  SRR19973070.341403/150‑1 (MQ=60)
                                                                                              TGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACG                                                         >  SRR19973070.3275483/1‑150 (MQ=60)
                                                                                                   atcctttaattatttttttttctTTTGCCTAACAATATTTGCAATATTATATTTAGATTTTTTGTGTGCGTTTGTGGAATCAGTTTTATCCAGTTAAACTAAAATGTCACATTGTCTAACGTAGTTCGTATCTTTATGATTAACGACAGT                                                    <  SRR19973070.819160/127‑1 (MQ=60)
                                                                                                      CTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGAC                                                 >  SRR19973070.758806/1‑150 (MQ=60)
                                                                                                      CTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGAC                                                 >  SRR19973070.761229/1‑150 (MQ=60)
                                                                                                      CTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGAC                                                 >  SRR19973070.2223152/1‑150 (MQ=60)
                                                                                                       TGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACA                                                >  SRR19973070.932783/1‑150 (MQ=60)
                                                                                                        GTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACAC                                               >  SRR19973070.397378/1‑150 (MQ=60)
                                                                                                         TAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCATATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCATTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACT                                              <  SRR19973070.450029/150‑1 (MQ=60)
                                                                                                             TATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCT                                          >  SRR19973070.2854077/1‑150 (MQ=60)
                                                                                                             TATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCT                                          >  SRR19973070.2766469/1‑150 (MQ=60)
                                                                                                              ATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTG                                         >  SRR19973070.560587/1‑150 (MQ=60)
                                                                                                                   TTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGGTAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACT                                    <  SRR19973070.1112232/150‑1 (MQ=60)
                                                                                                                   TTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACT                                    <  SRR19973070.1112194/150‑1 (MQ=60)
                                                                                                                   TTATTCGTTTGCCGAACAATATTTGCCAGATTATATCTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACT                                    >  SRR19973070.157264/1‑150 (MQ=60)
                                                                                                                    TATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTT                                   >  SRR19973070.941439/1‑150 (MQ=60)
                                                                                                                    TATTCGTTTGCCGAAAAATATTTGCCATATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCATTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTT                                   <  SRR19973070.3109725/150‑1 (MQ=60)
                                                                                                                     ATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTG                                  >  SRR19973070.2484176/1‑150 (MQ=60)
                                                                                                                       TCGTCTGCCGAACAATATTGGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTG                                <  SRR19973070.2623608/150‑1 (MQ=60)
                                                                                                                        CGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGC                               >  SRR19973070.2931467/1‑150 (MQ=60)
                                                                                                                         GTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCA                              >  SRR19973070.1724579/1‑150 (MQ=60)
                                                                                                                         GTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCA                              >  SRR19973070.1724578/1‑150 (MQ=60)
                                                                                                                              CCTAACAATATTGGCCAGATTATATTTAGCTTTATTGAGTGCGATAGGGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAT                         <  SRR19973070.1484141/150‑1 (MQ=60)
                                                                                                                              CCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAT                         >  SRR19973070.2106990/1‑150 (MQ=60)
                                                                                                                              CCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAT                         <  SRR19973070.1484031/150‑1 (MQ=60)
                                                                                                                              CCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAT                         >  SRR19973070.431450/1‑150 (MQ=60)
                                                                                                                              CCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAT                         >  SRR19973070.431497/1‑150 (MQ=60)
                                                                                                                               CGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATT                        <  SRR19973070.2286469/150‑1 (MQ=60)
                                                                                                                               CGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATT                        >  SRR19973070.2285360/1‑150 (MQ=60)
                                                                                                                               CGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATT                        >  SRR19973070.2285251/1‑150 (MQ=60)
                                                                                                                               CGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATT                        >  SRR19973070.1715130/1‑150 (MQ=60)
                                                                                                                                 AACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGG                      >  SRR19973070.3049964/1‑150 (MQ=60)
                                                                                                                                   CAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGAT                    >  SRR19973070.1544845/1‑150 (MQ=60)
                                                                                                                                   CAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGAT                    >  SRR19973070.1181625/1‑150 (MQ=60)
                                                                                                                                   CAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGAT                    <  SRR19973070.142564/150‑1 (MQ=60)
                                                                                                                                   CAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGAT                    <  SRR19973070.1842108/150‑1 (MQ=60)
                                                                                                                                    AATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATC                   >  SRR19973070.2948245/1‑150 (MQ=60)
                                                                                                                                    AATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATC                   >  SRR19973070.637110/1‑150 (MQ=60)
                                                                                                                                     ATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCT                  >  SRR19973070.2576328/1‑150 (MQ=60)
                                                                                                                                     ATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCT                  >  SRR19973070.2576271/1‑150 (MQ=60)
                                                                                                                                      TATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTG                 >  SRR19973070.2647607/1‑150 (MQ=60)
                                                                                                                                         ttcccttttttTTTTTAGCTTTATTGAGTGCGTTAGTGGAATCATTTTTATCCTTTGAAAATAAAATGTAACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCAT              <  SRR19973070.2517986/139‑1 (MQ=60)
                                                                                                                                         TTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCAT              >  SRR19973070.1645407/1‑150 (MQ=60)
                                                                                                                                         TTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCAT              <  SRR19973070.2517999/150‑1 (MQ=60)
                                                                                                                                           GCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTC            >  SRR19973070.1381658/1‑150 (MQ=60)
                                                                                                                                           GCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTC            >  SRR19973070.1381738/1‑150 (MQ=60)
                                                                                                                                           GCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTC            >  SRR19973070.1477568/1‑150 (MQ=60)
                                                                                                                                              AGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCC         >  SRR19973070.596182/1‑150 (MQ=60)
                                                                                                                                               GATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCG        >  SRR19973070.680096/1‑150 (MQ=60)
                                                                                                                                                    TATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATTTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACTACAGTGACACTACCTGAGACTTGTGCATAAATAGGATCTGCATTCCCCGAGACA   >  SRR19973070.2314288/1‑150 (MQ=60)
                                                                                                                                                    TATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACA   <  SRR19973070.2108239/150‑1 (MQ=60)
                                                                                                                                                     ATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACAT  >  SRR19973070.2876681/1‑150 (MQ=60)
                                                                                                                                                     ATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACAT  >  SRR19973070.3299729/1‑150 (MQ=60)
                                                                                                                                                     ATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACAT  >  SRR19973070.3299651/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AATAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACAT  >  CP009273/2476375‑2476673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑