Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,819,102 G→T 24.1% intergenic (‑167/‑89) mltB ← / → srlA membrane‑bound lytic murein transglycosylase B/glucitol/sorbitol‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,819,1020GT24.1% 718.3 / 167.7 285intergenic (‑167/‑89)mltB/srlAmembrane‑bound lytic murein transglycosylase B/glucitol/sorbitol‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base G (111/105);  new base T (33/36);  total (144/141)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 288 total aligned reads displayed.

ATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAG  >  CP009273/2818954‑2819251
                                                                                                                                                    |                                                                                                                                                     
aTTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGc                                                                                                                                                      <  1:647547/150‑1 (MQ=255)
   cAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGAt                                                                                                                                                   <  1:4336271/150‑1 (MQ=255)
   cAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGAt                                                                                                                                                   >  2:4614290/1‑150 (MQ=255)
        aGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCaaaa                                                                                                                                              >  1:4253836/1‑150 (MQ=255)
         ggggCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCAGTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAAt                                                                                                                                             <  1:1977350/150‑1 (MQ=255)
             cAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAaca                                                                                                                                         >  2:2655582/1‑150 (MQ=255)
               aGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACt                                                                                                                                       <  2:2572286/150‑1 (MQ=255)
                gTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACtt                                                                                                                                      >  2:1452254/1‑150 (MQ=255)
                gTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACtt                                                                                                                                      <  1:2623046/150‑1 (MQ=255)
                gTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATACCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATATTTCATTTTGCGATCAAAATAACACtt                                                                                                                                      >  2:2784882/1‑150 (MQ=255)
                  aTGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACtttt                                                                                                                                    <  1:818613/150‑1 (MQ=255)
                   tGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTa                                                                                                                                   >  2:1523769/1‑150 (MQ=255)
                   tGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTa                                                                                                                                   <  2:684804/150‑1 (MQ=255)
                     gTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTCTCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTaaa                                                                                                                                 >  2:868456/1‑150 (MQ=255)
                      tAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAAt                                                                                                                                >  1:2385481/1‑150 (MQ=255)
                       aaaGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATc                                                                                                                               >  1:1802881/1‑150 (MQ=255)
                          gCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCttt                                                                                                                            <  2:1408823/150‑1 (MQ=255)
                          gCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAAATCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCttt                                                                                                                            <  2:2786950/150‑1 (MQ=255)
                              cACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAAt                                                                                                                        <  2:4119863/150‑1 (MQ=255)
                              cACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAAt                                                                                                                        <  1:2458637/150‑1 (MQ=255)
                                cGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCt                                                                                                                      <  1:3920028/150‑1 (MQ=255)
                                cGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTAACTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCt                                                                                                                      <  1:3037966/150‑1 (MQ=255)
                                 gCCCCGCACCAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTg                                                                                                                     <  2:522080/150‑1 (MQ=255)
                                    ccGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTgatt                                                                                                                  <  1:2226190/150‑1 (MQ=255)
                                    ccGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTgatt                                                                                                                  <  1:4538768/150‑1 (MQ=255)
                                     cGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTgatta                                                                                                                 <  1:2911092/150‑1 (MQ=255)
                                     cGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTgatta                                                                                                                 <  1:468524/150‑1 (MQ=255)
                                      gCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTgattag                                                                                                                <  1:751257/150‑1 (MQ=255)
                                         caAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTgattagatt                                                                                                             >  1:1554890/1‑150 (MQ=255)
                                            ggAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAgg                                                                                                          >  2:3039453/1‑150 (MQ=255)
                                            ggAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAgg                                                                                                          >  2:3560282/1‑150 (MQ=255)
                                              aaGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGtt                                                                                                        <  2:1984096/150‑1 (MQ=255)
                                               aGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTg                                                                                                       >  2:7330/1‑150 (MQ=255)
                                                 cGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGcc                                                                                                     <  1:3896851/150‑1 (MQ=255)
                                                   gTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGt                                                                                                   >  2:1540174/1‑150 (MQ=255)
                                                   gTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGt                                                                                                   <  2:2341918/150‑1 (MQ=255)
                                                    tAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGtt                                                                                                  >  1:2182967/1‑150 (MQ=255)
                                                     aGTCACTGCCCGATACGGACTTTACATAACTCACCTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGttt                                                                                                 <  2:1327336/150‑1 (MQ=255)
                                                     aGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGttt                                                                                                 <  2:1595745/150‑1 (MQ=255)
                                                     aGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGttt                                                                                                 <  1:4076731/150‑1 (MQ=255)
                                                      gTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTg                                                                                                >  1:736693/1‑150 (MQ=255)
                                                             cccGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGACGGTTGGtaataa                                                                                         >  2:2418299/1‑150 (MQ=255)
                                                                 aTACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACa                                                                                     <  2:1841291/150‑1 (MQ=255)
                                                                      gACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTACATTTTGCGATCAAAATAACACTGTGAAATCTTTCAAGCTGATTAGAGTAGGTTGCCGTGTGCGAATAAAACAATaaa                                                                                >  1:2991697/1‑150 (MQ=255)
                                                                       aCTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAAt                                                                               >  1:1418106/1‑150 (MQ=255)
                                                                        cTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATc                                                                              <  1:3534684/150‑1 (MQ=255)
                                                                          ttACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCt                                                                            <  2:1075473/150‑1 (MQ=255)
                                                                           tACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTg                                                                           <  2:3323978/150‑1 (MQ=255)
                                                                            aCATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGa                                                                          >  2:3540470/1‑150 (MQ=255)
                                                                            aCATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGa                                                                          >  1:40357/1‑150 (MQ=255)
                                                                             cATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGaa                                                                         >  1:1658362/1‑150 (MQ=255)
                                                                                aactcaactcATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGga                                                                      >  1:3167593/1‑150 (MQ=255)
                                                                                 actcaactcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGgag                                                                     <  1:2109357/150‑1 (MQ=255)
                                                                                   tcaactcaTTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGgagag                                                                   <  2:64183/150‑1 (MQ=255)
                                                                                         caTTCCCCTCGCTAACCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGATAACCAt                                                             >  2:3932813/1‑150 (MQ=255)
                                                                                           ttCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGa                                                           <  1:1290766/150‑1 (MQ=255)
                                                                                           ttCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGa                                                           <  2:3818971/150‑1 (MQ=255)
                                                                                                      aTCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCAtt                                                >  2:4458206/1‑150 (MQ=255)
                                                                                                      aTCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCAtt                                                <  1:386231/150‑1 (MQ=255)
                                                                                                       tCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTa                                               <  1:2819333/150‑1 (MQ=255)
                                                                                                         cTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACt                                             <  2:188450/150‑1 (MQ=255)
                                                                                                            ttATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCAt                                          >  2:945995/1‑150 (MQ=255)
                                                                                                               ttCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGt                                       >  1:1812010/1‑150 (MQ=255)
                                                                                                               ttCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTCGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGt                                       <  2:3593905/150‑1 (MQ=255)
                                                                                                                taaaaCTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTg                                      >  2:2276422/3‑150 (MQ=255)
                                                                                                                 cAAACTTTCAAATTAAAATATTTATCTTTCATTATGCGAACAAAATAACACATTTAAATCTTTCAATCTGATTAGATTAGGTTGCCTTTAGGTAATAAAACAATAAATCCAGAAGGAGAGAACAATGATAGTAACCATTAATCATGGttc                                     >  2:4411280/1‑148 (MQ=255)
                                                                                                                    aCTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCaga                                  >  1:322205/1‑150 (MQ=255)
                                                                                                                    aCTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCaga                                  <  2:3767549/150‑1 (MQ=255)
                                                                                                                     cTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCagag                                 >  2:284890/1‑150 (MQ=255)
                                                                                                                     cTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCagag                                 >  1:154053/1‑150 (MQ=255)
                                                                                                                     cTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCagag                                 <  2:4373077/150‑1 (MQ=255)
                                                                                                                       ttCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTg                               <  2:3414187/150‑1 (MQ=255)
                                                                                                                       ttCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTg                               >  1:923471/1‑150 (MQ=255)
                                                                                                                        tCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTgg                              <  2:2816850/150‑1 (MQ=255)
                                                                                                                         cAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGt                             >  2:203214/1‑150 (MQ=255)
                                                                                                                         cAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGt                             >  1:1105999/1‑150 (MQ=255)
                                                                                                                         cAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGt                             >  1:1494205/1‑150 (MQ=255)
                                                                                                                           aaTTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGttt                           <  2:1554828/150‑1 (MQ=255)
                                                                                                                              tAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATc                        >  1:3562434/1‑150 (MQ=255)
                                                                                                                               aaaaTATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCg                       <  2:2150532/150‑1 (MQ=255)
                                                                                                                                 aaTATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCggg                     >  1:2504454/1‑150 (MQ=255)
                                                                                                                                  atatTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGc                    >  1:455903/1‑150 (MQ=255)
                                                                                                                                   tatTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCt                   >  1:4529841/1‑150 (MQ=255)
                                                                                                                                    atTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTg                  >  1:4244840/1‑150 (MQ=255)
                                                                                                                                    atTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTg                  <  1:3784345/150‑1 (MQ=255)
                                                                                                                                     tttATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGt                 >  2:2307748/1‑150 (MQ=255)
                                                                                                                                       tATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTc               <  2:1652884/150‑1 (MQ=255)
                                                                                                                                          cTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCaa            >  1:4542486/1‑150 (MQ=255)
                                                                                                                                            ttCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCTGAGTGGTTTATCGGGCTGTTGCaaaa          >  1:2708461/1‑150 (MQ=255)
                                                                                                                                            ttCATTTTGAGATCAAAATAACACTTTTAAATATTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCaaaa          <  1:584433/150‑1 (MQ=255)
                                                                                                                                              cATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTTATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAgg        >  2:2528549/1‑150 (MQ=255)
                                                                                                                                              cATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAgg        <  2:3655088/150‑1 (MQ=255)
                                                                                                                                              cATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAgg        >  2:3162260/1‑150 (MQ=255)
                                                                                                                                               aTTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAggg       >  2:1050985/1‑150 (MQ=255)
                                                                                                                                               aTTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAggg       >  1:1054737/1‑150 (MQ=255)
                                                                                                                                                ttttGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGc      <  1:3651464/150‑1 (MQ=255)
                                                                                                                                                 ttttCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCg     >  1:215384/1‑150 (MQ=255)
                                                                                                                                                 tttGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCg     <  1:2790568/150‑1 (MQ=255)
                                                                                                                                                   tGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGga   <  1:3349233/150‑1 (MQ=255)
                                                                                                                                                    gCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGgag  >  2:121540/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAG  >  CP009273/2818954‑2819251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A