Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,819,102 G→T 45.3% intergenic (‑167/‑89) mltB ← / → srlA membrane‑bound lytic murein transglycosylase B/glucitol/sorbitol‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,819,1020GT45.3% 209.7 / inf 260intergenic (‑167/‑89)mltB/srlAmembrane‑bound lytic murein transglycosylase B/glucitol/sorbitol‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base G (61/81);  new base T (64/53);  total (126/134)
Fisher's exact test for biased strand distribution p-value = 6.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 263 total aligned reads displayed.

GATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGA  >  CP009273/2818953‑2819250
                                                                                                                                                     |                                                                                                                                                    
gATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTg                                                                                                                                                      >  1:3975526/1‑150 (MQ=255)
  ttCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCCTTTTTCg                                                                                                                                                    <  1:2908571/150‑1 (MQ=255)
    cAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGAt                                                                                                                                                  <  1:4084772/150‑1 (MQ=255)
    cAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGAt                                                                                                                                                  <  2:214553/150‑1 (MQ=255)
    cAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGAt                                                                                                                                                  <  1:1975279/150‑1 (MQ=255)
     aaCCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATc                                                                                                                                                 <  1:940264/150‑1 (MQ=255)
     aaCCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATc                                                                                                                                                 <  1:3257784/150‑1 (MQ=255)
     aaCCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATc                                                                                                                                                 <  1:1999222/150‑1 (MQ=255)
       ccAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCaa                                                                                                                                               <  2:1754542/150‑1 (MQ=255)
       ccAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCaa                                                                                                                                               >  1:1883711/1‑150 (MQ=255)
        cAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCaaa                                                                                                                                              <  2:4266037/150‑1 (MQ=255)
          ggggCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAAt                                                                                                                                            <  1:70893/150‑1 (MQ=255)
          ggggCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAAt                                                                                                                                            >  1:2719899/1‑150 (MQ=255)
          ggggCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAAt                                                                                                                                            <  2:2818153/150‑1 (MQ=255)
                aGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACt                                                                                                                                      <  2:295659/150‑1 (MQ=255)
                 gTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACtt                                                                                                                                     <  1:1436101/150‑1 (MQ=255)
                     ggTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTaa                                                                                                                                 >  2:2523564/1‑150 (MQ=255)
                     ggTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATAAGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTaa                                                                                                                                 <  2:1377192/150‑1 (MQ=255)
                      gTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTaaa                                                                                                                                <  1:2731084/150‑1 (MQ=255)
                            cATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTc                                                                                                                          <  2:3294179/150‑1 (MQ=255)
                               cACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAAt                                                                                                                       >  1:1404572/1‑150 (MQ=255)
                                   ccccGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTga                                                                                                                   <  2:3556226/150‑1 (MQ=255)
                                    cccGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTAAATCTgat                                                                                                                  >  1:3087904/1‑150 (MQ=255)
                                    cccGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTgat                                                                                                                  <  2:1203376/150‑1 (MQ=255)
                                        cacaAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTgattaga                                                                                                              >  2:2225594/1‑150 (MQ=255)
                                         acaAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTgattagat                                                                                                             <  2:4151097/150‑1 (MQ=255)
                                         acaAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTgattagat                                                                                                             <  1:3938711/150‑1 (MQ=255)
                                          caAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTgattagatt                                                                                                            <  1:1132067/150‑1 (MQ=255)
                                           aaGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTgattagatta                                                                                                           >  1:1263891/1‑150 (MQ=255)
                                           aaGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTgattagatta                                                                                                           >  1:4225655/1‑150 (MQ=255)
                                            aGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGattagattag                                                                                                          >  2:3129424/1‑150 (MQ=255)
                                             ggAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAgg                                                                                                         >  1:2770839/1‑150 (MQ=255)
                                               aaGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGtt                                                                                                       <  2:3932764/150‑1 (MQ=255)
                                                  cGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGcc                                                                                                    >  2:4448719/1‑150 (MQ=255)
                                                  cGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGcc                                                                                                    <  1:516921/150‑1 (MQ=255)
                                                  cGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGcc                                                                                                    <  1:1499880/150‑1 (MQ=255)
                                                   ggTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCg                                                                                                   >  1:2072183/1‑150 (MQ=255)
                                                   ggTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCg                                                                                                   >  2:2609376/1‑150 (MQ=255)
                                                     tAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGtt                                                                                                 <  1:3109615/150‑1 (MQ=255)
                                                     tAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGtt                                                                                                 <  1:1529799/150‑1 (MQ=255)
                                                       gTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTg                                                                                               <  2:1574944/150‑1 (MQ=255)
                                                            tGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATTTTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGtaat                                                                                          <  1:4403071/150‑1 (MQ=255)
                                                            tGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGtaat                                                                                          <  1:4461809/150‑1 (MQ=255)
                                                             gCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGtaata                                                                                         >  2:3088331/1‑150 (MQ=255)
                                                              cccGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGtaataa                                                                                        >  1:2590728/1‑150 (MQ=255)
                                                                cGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATaaaa                                                                                      >  2:284833/1‑150 (MQ=255)
                                                                   tACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACaa                                                                                   >  1:1018070/1‑150 (MQ=255)
                                                                     cGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATa                                                                                 <  2:385158/150‑1 (MQ=255)
                                                                     cGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATa                                                                                 <  2:3975446/150‑1 (MQ=255)
                                                                         cTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATc                                                                             <  2:1626795/150‑1 (MQ=255)
                                                                          tttACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATcc                                                                            >  1:278444/1‑150 (MQ=255)
                                                                                    tcaactcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGgagag                                                                  <  2:1883711/150‑1 (MQ=255)
                                                                                    tcaactcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGgagag                                                                  >  1:3532355/1‑150 (MQ=255)
                                                                                     caactcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGgagaga                                                                 >  2:3847043/1‑150 (MQ=255)
                                                                                      aactcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGaa                                                                >  1:3031321/1‑150 (MQ=255)
                                                                                       actcaTTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATATTAAGATCAAAATAAAACTATTAAATATTAAAATATGATAAGATTAGGTTGCCGTTTGGAAATAAAACAATAAATCATTAAGGATAGAAc                                                               >  2:1071235/1‑150 (MQ=255)
                                                                                        ctcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACa                                                              <  2:2564002/150‑1 (MQ=255)
                                                                                        ctcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACa                                                              >  1:1099636/1‑150 (MQ=255)
                                                                                        ctcaTTCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATATTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAAGAAATCCTGAAGGAGAGAACa                                                              >  1:1340937/1‑150 (MQ=255)
                                                                                         tcaTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACaa                                                             <  1:3333435/150‑1 (MQ=255)
                                                                                          caTTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAAt                                                            >  1:3365808/1‑150 (MQ=255)
                                                                                             tCCCCTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGAt                                                         >  2:3660085/1‑150 (MQ=255)
                                                                                               cccTCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTCATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAg                                                       <  1:614744/150‑1 (MQ=255)
                                                                                                ccTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGa                                                      <  1:4561560/150‑1 (MQ=255)
                                                                                                 cTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAAACTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGaa                                                     >  2:2047076/1‑150 (MQ=255)
                                                                                                  tCGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGaaa                                                    >  1:2172051/1‑150 (MQ=255)
                                                                                                   cGCTATCCTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAAc                                                   <  1:361604/150‑1 (MQ=255)
                                                                                                      tATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCAt                                                >  2:54514/1‑150 (MQ=255)
                                                                                                      tATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCAt                                                >  1:146097/1‑150 (MQ=255)
                                                                                                       aTCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCAtt                                               <  1:4318380/150‑1 (MQ=255)
                                                                                                          cTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACt                                            >  2:4498352/1‑150 (MQ=255)
                                                                                                          cTTTTATTAAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACt                                            >  2:3020132/1‑150 (MQ=255)
                                                                                                             ttATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCAt                                         <  1:1304479/150‑1 (MQ=255)
                                                                                                              tATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATg                                        <  1:4066878/150‑1 (MQ=255)
                                                                                                               aTTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATgg                                       >  2:3427920/1‑150 (MQ=255)
                                                                                                                ttCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGt                                      >  2:1456145/1‑150 (MQ=255)
                                                                                                                  aaaaCTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGc                                    >  2:2811389/2‑150 (MQ=255)
                                                                                                                  aaaaCTTTCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGc                                    >  1:2844079/2‑150 (MQ=255)
                                                                                                                   aaaCTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCa                                   >  2:2774433/1‑150 (MQ=255)
                                                                                                                   aaaCTTTCAAAATAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCa                                   <  2:2646069/150‑1 (MQ=255)
                                                                                                                       tttCAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGt                               >  1:3832147/1‑150 (MQ=255)
                                                                                                                          cAAATTAAAATATTTATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGt                            <  1:2097667/150‑1 (MQ=255)
                                                                                                                           aaaTTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGtt                           <  1:3185886/150‑1 (MQ=255)
                                                                                                                            aaTTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGttt                          >  1:1808191/1‑150 (MQ=255)
                                                                                                                                 aaaTATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCgg                     <  2:4394534/150‑1 (MQ=255)
                                                                                                                                 aaaTATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCgg                     <  2:4394518/150‑1 (MQ=255)
                                                                                                                                     atTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTg                 <  2:1679765/150‑1 (MQ=255)
                                                                                                                                      tttATCTTTCTTTTTGCGATCAAAATAAAACTTTGAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGt                <  1:1291532/150‑1 (MQ=255)
                                                                                                                                       ttATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGTAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGtt               <  2:804416/150‑1 (MQ=255)
                                                                                                                                        tATCTTTCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTc              <  2:333735/150‑1 (MQ=255)
                                                                                                                                        tATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTc              >  1:2325904/1‑150 (MQ=255)
                                                                                                                                         aTCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTcc             >  2:1786644/1‑150 (MQ=255)
                                                                                                                                          tCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCa            <  1:3774270/150‑1 (MQ=255)
                                                                                                                                            tttCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGATTGGTTTATCGGGCTGTTACaaa          >  2:3160811/1‑150 (MQ=255)
                                                                                                                                             ttCATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCaaaa         >  1:2505103/1‑150 (MQ=255)
                                                                                                                                               cATTTTTCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAgg       >  1:2693463/1‑150 (MQ=255)
                                                                                                                                                  ttttCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCg    >  1:1163710/1‑150 (MQ=255)
                                                                                                                                                  tttGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCg    >  1:3657617/1‑150 (MQ=255)
                                                                                                                                                    tGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGga  >  1:1727533/1‑150 (MQ=255)
                                                                                                                                                    tGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGga  >  1:343216/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTCACTGCCCGATACGGACTTTACATAACTCAACTCATTCCCCTCGCTATCCTTTTATTCAAACTTTCAAATTAAAATATTTATCTTTCATTTTGCGATCAAAATAACACTTTTAAATCTTTCAATCTGATTAGATTAGGTTGCCGTTTGGTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGA  >  CP009273/2818953‑2819250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A