Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,097,262 C→T 91.5% F297F (TTC→TTT)  mutY → adenine DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,097,2620CT91.5% 813.1 / 53.1 259F297F (TTC→TTT) mutYadenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base C (14/8);  new base T (103/133);  total (118/141)
Fisher's exact test for biased strand distribution p-value = 7.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 259 total aligned reads displayed.

GCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCAC  >  CP009273/3097115‑3097408
                                                                                                                                                   |                                                                                                                                                  
gCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCa                                                                                                                                                  <  1:1526875/150‑1 (MQ=255)
gCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCa                                                                                                                                                  <  1:2144316/150‑1 (MQ=255)
      gccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGa                                                                                                                                            >  1:4592422/1‑150 (MQ=255)
      gccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGa                                                                                                                                            <  1:56628/150‑1 (MQ=255)
       ccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGat                                                                                                                                           <  2:853957/150‑1 (MQ=255)
       ccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGat                                                                                                                                           <  2:710207/150‑1 (MQ=255)
         gAGCGGATTGTGGGGCGGTTTATTCTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCTGTGGTTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGatat                                                                                                                                         <  1:2912057/150‑1 (MQ=255)
           gCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATtg                                                                                                                                       <  1:3980101/150‑1 (MQ=255)
             ggATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATtgtg                                                                                                                                     <  2:2352329/150‑1 (MQ=255)
             ggATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATtgtg                                                                                                                                     <  1:2600080/150‑1 (MQ=255)
              gATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGc                                                                                                                                    >  2:3965347/1‑150 (MQ=255)
               aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGcc                                                                                                                                   <  1:3344186/150‑1 (MQ=255)
                ttGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCt                                                                                                                                  <  2:1895695/150‑1 (MQ=255)
                ttGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCt                                                                                                                                  <  2:419247/150‑1 (MQ=255)
                  gtgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTAt                                                                                                                                <  1:523042/150‑1 (MQ=255)
                        cGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCt                                                                                                                          <  2:4059413/150‑1 (MQ=255)
                        cGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCt                                                                                                                          >  2:4086190/1‑150 (MQ=255)
                         ggTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCtt                                                                                                                         <  2:546902/150‑1 (MQ=255)
                         ggTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCtt                                                                                                                         <  1:104766/150‑1 (MQ=255)
                           tttATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTcc                                                                                                                       <  1:457490/150‑1 (MQ=255)
                               taCTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCgtg                                                                                                                   <  1:2982307/150‑1 (MQ=255)
                               taCTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCgtg                                                                                                                   <  1:3508431/150‑1 (MQ=255)
                                aCTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCgtgt                                                                                                                  <  1:270249/150‑1 (MQ=255)
                                 cTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTgtc                                                                                                                 <  2:1927248/150‑1 (MQ=255)
                                  tGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGTGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTgtcg                                                                                                                <  1:3165685/150‑1 (MQ=255)
                                     ttCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCa                                                                                                             <  2:491605/150‑1 (MQ=255)
                                     ttCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCa                                                                                                             <  2:3783866/150‑1 (MQ=255)
                                       cccGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCAtt                                                                                                           >  1:4320933/1‑150 (MQ=255)
                                         cGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCa                                                                                                         >  2:2097001/1‑150 (MQ=255)
                                            aGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCACCGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCg                                                                                                      <  2:2192596/150‑1 (MQ=255)
                                            aGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCg                                                                                                      <  2:509718/150‑1 (MQ=255)
                                            aGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCg                                                                                                      >  1:625796/1‑150 (MQ=255)
                                                  ccGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCa                                                                                                >  2:2490619/1‑150 (MQ=255)
                                                       gaagaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGAt                                                                                           >  2:3606534/1‑150 (MQ=255)
                                                        aagaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATg                                                                                          >  1:2250651/1‑150 (MQ=255)
                                                         agaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGa                                                                                         >  1:3586913/1‑150 (MQ=255)
                                                           aaaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCTCCGGCTGCATGGATGAAg                                                                                       >  2:3856588/1‑150 (MQ=255)
                                                           aaaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAg                                                                                       <  1:1642773/150‑1 (MQ=255)
                                                            aaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAgg                                                                                      >  1:1486726/1‑150 (MQ=255)
                                                             aGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGc                                                                                     >  1:3526404/1‑150 (MQ=255)
                                                             aGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGc                                                                                     >  1:2501742/1‑150 (MQ=255)
                                                                ttGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAAt                                                                                  <  1:797802/150‑1 (MQ=255)
                                                                       aGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGctct                                                                           <  2:3215999/150‑1 (MQ=255)
                                                                        gTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTg                                                                          <  2:2563198/150‑1 (MQ=255)
                                                                             tggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGtata                                                                     <  2:4243351/150‑1 (MQ=255)
                                                                              ggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATaa                                                                    >  1:4323647/1‑150 (MQ=255)
                                                                                cgcAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACt                                                                  <  1:874550/150‑1 (MQ=255)
                                                                                cgcAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACt                                                                  <  1:716419/150‑1 (MQ=255)
                                                                                cgcAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACt                                                                  <  1:2503063/150‑1 (MQ=255)
                                                                                 gcAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACtt                                                                 >  2:1890689/1‑150 (MQ=255)
                                                                                  cAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCTCTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTa                                                                <  2:1228740/150‑1 (MQ=255)
                                                                                  cAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTa                                                                <  2:4302721/150‑1 (MQ=255)
                                                                                  cAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTa                                                                >  2:733422/1‑150 (MQ=255)
                                                                                   aaCGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAg                                                               <  2:13298/150‑1 (MQ=255)
                                                                                   aaCGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAg                                                               >  2:1736838/1‑150 (MQ=255)
                                                                                    aCGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAgc                                                              <  1:3861914/150‑1 (MQ=255)
                                                                                      ggCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAgcgc                                                            >  2:3090800/1‑150 (MQ=255)
                                                                                      ggCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAgcgc                                                            >  2:923176/1‑150 (MQ=255)
                                                                                         aGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAc                                                         >  2:823415/1‑150 (MQ=255)
                                                                                          gATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAcc                                                        >  2:475246/1‑150 (MQ=255)
                                                                                              gctgcCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAccgccg                                                    <  1:4624462/150‑1 (MQ=255)
                                                                                               ctgcCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGt                                                   <  2:906535/150‑1 (MQ=255)
                                                                                               ctgcCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGt                                                   <  2:3229640/150‑1 (MQ=255)
                                                                                                tgcCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTc                                                  <  2:4008165/150‑1 (MQ=255)
                                                                                                  ccGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAg                                                >  1:1164715/1‑150 (MQ=255)
                                                                                                  ccGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAg                                                >  1:1551175/1‑150 (MQ=255)
                                                                                                   cGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGt                                               >  2:2455462/1‑150 (MQ=255)
                                                                                                   cGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGt                                               <  1:2391919/150‑1 (MQ=255)
                                                                                                   cGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCAGTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGt                                               >  2:1204223/1‑150 (MQ=255)
                                                                                                    gATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGtt                                              >  2:1755449/1‑150 (MQ=255)
                                                                                                       aaCCTGACGCAACTGACCGCGTTTCGGCATGCCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGc                                           >  1:2380529/1‑150 (MQ=255)
                                                                                                         ccTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCt                                         <  1:3140740/150‑1 (MQ=255)
                                                                                                         ccTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCt                                         >  2:1571801/1‑150 (MQ=255)
                                                                                                            gACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGc                                      >  2:4538657/1‑150 (MQ=255)
                                                                                                                 aaCTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTc                                 <  2:4393360/150‑1 (MQ=255)
                                                                                                                 aaCTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTc                                 <  1:2953607/150‑1 (MQ=255)
                                                                                                                 aaCTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTc                                 <  1:3503091/150‑1 (MQ=255)
                                                                                                                  aCTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTCGATATTGTGCCTATGTGGCGTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTcc                                <  2:2054877/150‑1 (MQ=255)
                                                                                                                  aCTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTcc                                <  2:3202397/150‑1 (MQ=255)
                                                                                                                    tGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCg                              >  1:2818148/1‑150 (MQ=255)
                                                                                                                    tGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCg                              >  1:3271850/1‑150 (MQ=255)
                                                                                                                     gACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGt                             >  1:3326567/1‑150 (MQ=255)
                                                                                                                            tttCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGt                      >  2:2919377/1‑150 (MQ=255)
                                                                                                                              tCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGttt                    >  1:192017/1‑150 (MQ=255)
                                                                                                                              tCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGttt                    <  1:2951653/150‑1 (MQ=255)
                                                                                                                                  cATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTa                <  2:1195084/150‑1 (MQ=255)
                                                                                                                                   aTACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAc               >  2:2949302/1‑150 (MQ=255)
                                                                                                                                     aCCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAcag             <  1:2676206/150‑1 (MQ=255)
                                                                                                                                      ccTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAcagc            >  2:1137682/1‑150 (MQ=255)
                                                                                                                                       cTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAcagca           >  2:1664014/1‑150 (MQ=255)
                                                                                                                                         tCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGt         <  2:3144745/150‑1 (MQ=255)
                                                                                                                                          cAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGtt        >  2:2906102/1‑150 (MQ=255)
                                                                                                                                          cAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGtt        <  1:1870143/150‑1 (MQ=255)
                                                                                                                                           aGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTa       >  2:4078373/1‑150 (MQ=255)
                                                                                                                                           aGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTa       <  2:1339112/150‑1 (MQ=255)
                                                                                                                                           aGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTATGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTa       >  1:1382490/1‑150 (MQ=255)
                                                                                                                                            gCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTAc      <  1:4094674/150‑1 (MQ=255)
                                                                                                                                             ccATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACg     <  2:2155304/150‑1 (MQ=255)
                                                                                                                                             ccATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACg     <  2:1523638/150‑1 (MQ=255)
                                                                                                                                                tttCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCAc  >  1:2066893/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCAC  >  CP009273/3097115‑3097408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGG  >  CP009273/3097114‑3097411
                                                                                                                                                    |                                                                                                                                                     
AGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTC                                                                                                                                                      <  SRR19973072.1232392/150‑1 (MQ=60)
 GCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCA                                                                                                                                                     <  SRR19973072.2148982/150‑1 (MQ=60)
 GCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCA                                                                                                                                                     <  SRR19973072.1530112/150‑1 (MQ=60)
 GCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCA                                                                                                                                                     <  SRR19973072.1530114/150‑1 (MQ=60)
 GCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCA                                                                                                                                                     <  SRR19973072.1530115/150‑1 (MQ=60)
   GTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACT                                                                                                                                                   <  SRR19973072.4641970/150‑1 (MQ=60)
     CCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTA                                                                                                                                                 <  SRR19973072.3350773/150‑1 (MQ=60)
      CGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAG                                                                                                                                                >  SRR19973072.863762/1‑150 (MQ=60)
       GCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGA                                                                                                                                               >  SRR19973072.4602064/1‑150 (MQ=60)
       GCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGA                                                                                                                                               <  SRR19973072.56780/150‑1 (MQ=60)
       GCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGA                                                                                                                                               <  SRR19973072.56795/150‑1 (MQ=60)
       GCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGA                                                                                                                                               <  SRR19973072.56913/150‑1 (MQ=60)
        CCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGAT                                                                                                                                              <  SRR19973072.744176/150‑1 (MQ=60)
          GAGCGGATTGTGGGGCGGTTTATTCTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCTGTGGTTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATAT                                                                                                                                            <  SRR19973072.2918296/150‑1 (MQ=60)
          GAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGTTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATAT                                                                                                                                            <  SRR19973072.2918576/150‑1 (MQ=60)
           AGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATT                                                                                                                                           >  SRR19973072.3273694/1‑150 (MQ=60)
            GCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTG                                                                                                                                          <  SRR19973072.4377122/150‑1 (MQ=60)
            GCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTG                                                                                                                                          <  SRR19973072.3988433/150‑1 (MQ=60)
              GGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTG                                                                                                                                        <  SRR19973072.2605679/150‑1 (MQ=60)
                ATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCC                                                                                                                                      <  SRR19973072.3351204/150‑1 (MQ=60)
                ATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCC                                                                                                                                      <  SRR19973072.3351278/150‑1 (MQ=60)
                  TGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTA                                                                                                                                    <  SRR19973072.3526293/150‑1 (MQ=60)
                  TGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTA                                                                                                                                    >  SRR19973072.517205/1‑150 (MQ=60)
                   GTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTAT                                                                                                                                   <  SRR19973072.524290/150‑1 (MQ=60)
                    TGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATG                                                                                                                                  >  SRR19973072.272643/1‑150 (MQ=60)
                    TGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATG                                                                                                                                  >  SRR19973072.2702845/1‑150 (MQ=60)
                          GGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTT                                                                                                                            <  SRR19973072.105028/150‑1 (MQ=60)
                            TTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCC                                                                                                                          <  SRR19973072.458932/150‑1 (MQ=60)
                            TTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCC                                                                                                                          <  SRR19973072.458553/150‑1 (MQ=60)
                            TTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCC                                                                                                                          >  SRR19973072.2294761/1‑150 (MQ=60)
                             TTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCC                                                                                                                         <  SRR19973072.365728/150‑1 (MQ=60)
                              TATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCG                                                                                                                        >  SRR19973072.1342043/1‑150 (MQ=60)
                                TACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTG                                                                                                                      <  SRR19973072.3515802/150‑1 (MQ=60)
                                TACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTG                                                                                                                      <  SRR19973072.3515790/150‑1 (MQ=60)
                                TACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTG                                                                                                                      <  SRR19973072.2988664/150‑1 (MQ=60)
                                 ACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGT                                                                                                                     <  SRR19973072.270887/150‑1 (MQ=60)
                                  CTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTC                                                                                                                    <  SRR19973072.1567661/150‑1 (MQ=60)
                                   TGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGTGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCG                                                                                                                   <  SRR19973072.3172366/150‑1 (MQ=60)
                                   TGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGTGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCG                                                                                                                   <  SRR19973072.3172357/150‑1 (MQ=60)
                                   TGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCG                                                                                                                   >  SRR19973072.1221167/1‑150 (MQ=60)
                                      TTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCA                                                                                                                <  SRR19973072.3460881/150‑1 (MQ=60)
                                       TCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCAT                                                                                                               >  SRR19973072.2117679/1‑150 (MQ=60)
                                        CCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATT                                                                                                              >  SRR19973072.4329969/1‑150 (MQ=60)
                                         CCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTC                                                                                                             <  SRR19973072.150108/150‑1 (MQ=60)
                                             AGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCG                                                                                                         <  SRR19973072.4592328/150‑1 (MQ=60)
                                             AGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCG                                                                                                         >  SRR19973072.627228/1‑150 (MQ=60)
                                                  GCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGC                                                                                                    >  SRR19973072.302829/1‑150 (MQ=60)
                                                    CGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCAT                                                                                                  >  SRR19973072.2590098/1‑150 (MQ=60)
                                                    CGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCAT                                                                                                  <  SRR19973072.4119794/150‑1 (MQ=60)
                                                       CGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATCCACCGGCTGCATGGA                                                                                               >  SRR19973072.2984950/1‑150 (MQ=60)
                                                         AAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATG                                                                                             >  SRR19973072.2255499/1‑150 (MQ=60)
                                                         AAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATG                                                                                             >  SRR19973072.2255488/1‑150 (MQ=60)
                                                          AGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGA                                                                                            >  SRR19973072.3594462/1‑150 (MQ=60)
                                                            AAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAG                                                                                          <  SRR19973072.1646358/150‑1 (MQ=60)
                                                            AAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAG                                                                                          <  SRR19973072.1646435/150‑1 (MQ=60)
                                                             AAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGG                                                                                         >  SRR19973072.1489901/1‑150 (MQ=60)
                                                              AGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGC                                                                                        >  SRR19973072.2507181/1‑150 (MQ=60)
                                                              AGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGC                                                                                        >  SRR19973072.3533810/1‑150 (MQ=60)
                                                              AGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGC                                                                                        >  SRR19973072.3533854/1‑150 (MQ=60)
                                                              AGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGC                                                                                        >  SRR19973072.2507120/1‑150 (MQ=60)
                                                              AGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGC                                                                                        >  SRR19973072.2507224/1‑150 (MQ=60)
                                                                 TTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAAT                                                                                     <  SRR19973072.799504/150‑1 (MQ=60)
                                                                  TGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATG                                                                                    >  SRR19973072.2478353/1‑150 (MQ=60)
                                                                  TGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATG                                                                                    >  SRR19973072.3601768/1‑150 (MQ=60)
                                                                   GCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGC                                                                                   <  SRR19973072.26793/150‑1 (MQ=60)
                                                                    CGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCG                                                                                  <  SRR19973072.889015/150‑1 (MQ=60)
                                                                      GCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCT                                                                                <  SRR19973072.3115610/150‑1 (MQ=60)
                                                                        AGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCT                                                                              <  SRR19973072.2183593/150‑1 (MQ=60)
                                                                           GGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGT                                                                           <  SRR19973072.1625158/150‑1 (MQ=60)
                                                                             CTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTAT                                                                         >  SRR19973072.3897741/1‑150 (MQ=60)
                                                                               GGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAA                                                                       >  SRR19973072.4332656/1‑150 (MQ=60)
                                                                               GGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAA                                                                       >  SRR19973072.4332689/1‑150 (MQ=60)
                                                                                 CGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACT                                                                     <  SRR19973072.2508506/150‑1 (MQ=60)
                                                                                 CGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACT                                                                     <  SRR19973072.717953/150‑1 (MQ=60)
                                                                                 CGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACT                                                                     <  SRR19973072.717973/150‑1 (MQ=60)
                                                                                 CGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACT                                                                     <  SRR19973072.876394/150‑1 (MQ=60)
                                                                                 CGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACT                                                                     <  SRR19973072.876426/150‑1 (MQ=60)
                                                                                 CGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACT                                                                     <  SRR19973072.3204246/150‑1 (MQ=60)
                                                                                  GCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGTGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTT                                                                    <  SRR19973072.1807184/150‑1 (MQ=60)
                                                                                    AACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAG                                                                  <  SRR19973072.2541027/150‑1 (MQ=60)
                                                                                     ACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGC                                                                 <  SRR19973072.2029435/150‑1 (MQ=60)
                                                                                     ACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGC                                                                 <  SRR19973072.3869951/150‑1 (MQ=60)
                                                                                      CGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCG                                                                <  SRR19973072.2621861/150‑1 (MQ=60)
                                                                                       GGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGC                                                               >  SRR19973072.28571/1‑150 (MQ=60)
                                                                                          AGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAC                                                            >  SRR19973072.2259073/1‑150 (MQ=60)
                                                                                          AGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAC                                                            <  SRR19973072.3973641/150‑1 (MQ=60)
                                                                                             TTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGC                                                         <  SRR19973072.4166085/150‑1 (MQ=60)
                                                                                               GCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCG                                                       <  SRR19973072.1394452/150‑1 (MQ=60)
                                                                                               GCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCG                                                       <  SRR19973072.4634148/150‑1 (MQ=60)
                                                                                                CTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGT                                                      <  SRR19973072.3514745/150‑1 (MQ=60)
                                                                                                   CCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAG                                                   >  SRR19973072.1167151/1‑150 (MQ=60)
                                                                                                   CCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAG                                                   >  SRR19973072.1167152/1‑150 (MQ=60)
                                                                                                   CCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAG                                                   >  SRR19973072.1554465/1‑150 (MQ=60)
                                                                                                    CGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGT                                                  <  SRR19973072.2397092/150‑1 (MQ=60)
                                                                                                    CGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGT                                                  >  SRR19973072.4092154/1‑150 (MQ=60)
                                                                                                     GATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTT‑CACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTG                                                >  SRR19973072.74415/1‑150 (MQ=60)
                                                                                                        AACCTGACGCAACTGACCGCGTTTCGGCATGCCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGC                                              >  SRR19973072.2385661/1‑150 (MQ=60)
                                                                                                        AACCTGACGCAACTGACCGCGTTTCGGCATGCCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGC                                              >  SRR19973072.2385917/1‑150 (MQ=60)
                                                                                                          CCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCT                                            <  SRR19973072.3147362/150‑1 (MQ=60)
                                                                                                                  AACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTC                                    <  SRR19973072.3510434/150‑1 (MQ=60)
                                                                                                                  AACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTC                                    >  SRR19973072.3298521/1‑150 (MQ=60)
                                                                                                                  AACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTC                                    <  SRR19973072.2959918/150‑1 (MQ=60)
                                                                                                                     TGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCG                                 >  SRR19973072.3278798/1‑150 (MQ=60)
                                                                                                                     TGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCG                                 >  SRR19973072.2824184/1‑150 (MQ=60)
                                                                                                                      GACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGT                                >  SRR19973072.3333632/1‑150 (MQ=60)
                                                                                                                      GACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGGGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGT                                <  SRR19973072.4353216/150‑1 (MQ=60)
                                                                                                                        CCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGG                              <  SRR19973072.2743109/150‑1 (MQ=60)
                                                                                                                               TCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTT                       <  SRR19973072.2957964/150‑1 (MQ=60)
                                                                                                                               TCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTT                       >  SRR19973072.192504/1‑150 (MQ=60)
                                                                                                                               TCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTT                       >  SRR19973072.192491/1‑150 (MQ=60)
                                                                                                                               TCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTT                       <  SRR19973072.2957955/150‑1 (MQ=60)
                                                                                                                               TCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTT                       <  SRR19973072.2957965/150‑1 (MQ=60)
                                                                                                                                      ACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAG                <  SRR19973072.2681915/150‑1 (MQ=60)
                                                                                                                                      ACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAG                >  SRR19973072.547243/1‑150 (MQ=60)
                                                                                                                                       CCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGC               >  SRR19973072.3463665/1‑150 (MQ=60)
                                                                                                                                         TTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAG             >  SRR19973072.4602114/1‑150 (MQ=60)
                                                                                                                                           CAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTT           <  SRR19973072.1874166/150‑1 (MQ=60)
                                                                                                                                            AGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTATGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTA          >  SRR19973072.1385503/1‑150 (MQ=60)
                                                                                                                                             GCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTAC         <  SRR19973072.4103273/150‑1 (MQ=60)
                                                                                                                                              CCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACG        >  SRR19973072.2965776/1‑150 (MQ=60)
                                                                                                                                                 TTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCAC     >  SRR19973072.2071369/1‑150 (MQ=60)
                                                                                                                                                 TTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCAC     >  SRR19973072.2071391/1‑150 (MQ=60)
                                                                                                                                                    TCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGG  <  SRR19973072.1423371/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGG  >  CP009273/3097114‑3097411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑