Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,097,262 C→T 100% F297F (TTC→TTT)  mutY → adenine DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,097,2620CT95.9% 470.5 / 8.0 147F297F (TTC→TTT) mutYadenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/6);  new base T (62/79);  total (62/85)
Fisher's exact test for biased strand distribution p-value = 3.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 148 total aligned reads displayed.

TCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGC  >  CP009273/3097118‑3097406
                                                                                                                                                |                                                                                                                                                
tCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCCTTTTCAc                                                                                                                                               >  1:2735250/1‑148 (MQ=255)
 ccgccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACt                                                                                                                                              >  2:765671/1‑148 (MQ=255)
 ccgccgAGCGGATTGTGGGGCGGTTTATACTGTTACCCGCAGTTTGCCGACGAAGAAAGTTTGCTGCAGTGCCTGGAGCAACGGCAGATTGCTGCCGATAACATGACGCAACTGACCGCGTTTCGGCATACATTCAGCCATTTTCACt                                                                                                                                              >  2:443364/1‑148 (MQ=255)
  cgccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACtt                                                                                                                                             >  2:132343/1‑148 (MQ=255)
   gccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTa                                                                                                                                            <  2:1277160/148‑1 (MQ=255)
    ccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAg                                                                                                                                           <  1:1443496/148‑1 (MQ=255)
     cgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGa                                                                                                                                          <  2:2956773/148‑1 (MQ=255)
     cgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTCTCGGCATACCTTCAGCCATTTTCACTTAGa                                                                                                                                          <  2:2888800/148‑1 (MQ=255)
      gAGCGGATTGTGGTGCGGTTTATACTGTTTCGCGCAGTTTGCCGACGAAGAAAGTTTGCGTCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGat                                                                                                                                         <  2:2889666/148‑1 (MQ=255)
      gAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGat                                                                                                                                         <  2:2291267/148‑1 (MQ=255)
        gCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGatat                                                                                                                                       <  1:2145983/148‑1 (MQ=255)
            aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATtgtg                                                                                                                                   >  2:1922808/1‑148 (MQ=255)
              tgtgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGcc                                                                                                                                 <  2:745087/148‑1 (MQ=255)
                 ggggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTAt                                                                                                                              <  1:1821635/148‑1 (MQ=255)
                 ggggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTAt                                                                                                                              <  1:1858294/148‑1 (MQ=255)
                 ggggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTAt                                                                                                                              <  2:1444855/148‑1 (MQ=255)
                    gCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATGTTCACTTAGATAGTGTGCCTAggtg                                                                                                                           >  2:1497353/1‑148 (MQ=255)
                     cGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTgg                                                                                                                          <  1:2843698/148‑1 (MQ=255)
                       gTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCt                                                                                                                        <  2:209400/148‑1 (MQ=255)
                       gTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCt                                                                                                                        <  2:1155558/148‑1 (MQ=255)
                         ttATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTc                                                                                                                      <  1:2156785/148‑1 (MQ=255)
                         ttATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTc                                                                                                                      >  2:2424059/1‑148 (MQ=255)
                         ttATACTGTGTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTc                                                                                                                      <  1:3394983/148‑1 (MQ=255)
                              cTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCgtg                                                                                                                 <  1:1325841/148‑1 (MQ=255)
                              cTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCgtg                                                                                                                 >  2:194781/1‑148 (MQ=255)
                              cTGTGTCCCGCAGTTTGCCGTCGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTGTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCGTCCCgtg                                                                                                                 <  1:2304970/148‑1 (MQ=255)
                               tGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCgtgt                                                                                                                <  1:398972/148‑1 (MQ=255)
                                gTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTgtc                                                                                                               >  1:2571555/1‑148 (MQ=255)
                                gTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTgtc                                                                                                               >  1:2571567/1‑148 (MQ=255)
                                   tCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTGTCGGCATACCTTCAGCCAGTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTgtcgtc                                                                                                            >  2:2978601/1‑148 (MQ=255)
                                    cccGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCa                                                                                                           <  2:242890/148‑1 (MQ=255)
                                      cGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCAtt                                                                                                         >  1:358105/1‑148 (MQ=255)
                                      cGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCAtt                                                                                                         <  1:1450412/148‑1 (MQ=255)
                                        cAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGACAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCa                                                                                                       >  2:1129654/1‑148 (MQ=255)
                                            ttGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCgg                                                                                                   <  1:483967/148‑1 (MQ=255)
                                            ttGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCgg                                                                                                   <  1:2998235/148‑1 (MQ=255)
                                            ttGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCgg                                                                                                   >  1:1112506/1‑148 (MQ=255)
                                            ttGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCgg                                                                                                   <  1:978189/148‑1 (MQ=255)
                                            ttGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCgg                                                                                                   <  1:2630507/148‑1 (MQ=255)
                                                  acgaAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCAt                                                                                             <  2:2080152/148‑1 (MQ=255)
                                                   cgaAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATg                                                                                            >  1:2748583/1‑148 (MQ=255)
                                                    gaagaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATgg                                                                                           >  1:639966/1‑148 (MQ=255)
                                                    gaagaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATCGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATgg                                                                                           <  2:2596797/148‑1 (MQ=255)
                                                     aagatAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGa                                                                                          <  1:2664375/148‑1 (MQ=255)
                                                     aagaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGa                                                                                          <  2:2705954/148‑1 (MQ=255)
                                                     aagaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGa                                                                                          <  1:1239115/148‑1 (MQ=255)
                                                      agaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGAt                                                                                         >  1:3063287/1‑148 (MQ=255)
                                                       gaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATg                                                                                        <  2:3043007/148‑1 (MQ=255)
                                                        aaaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGa                                                                                       >  2:2833009/1‑148 (MQ=255)
                                                         aaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGaa                                                                                      >  1:2643147/1‑148 (MQ=255)
                                                          aGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAg                                                                                     >  2:2269985/1‑148 (MQ=255)
                                                          aGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAg                                                                                     >  2:144340/1‑148 (MQ=255)
                                                           gTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAgg                                                                                    <  2:3373057/148‑1 (MQ=255)
                                                             ttGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCa                                                                                  >  1:1877430/1‑148 (MQ=255)
                                                              tGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCaa                                                                                 <  2:979828/148‑1 (MQ=255)
                                                              tGCGGCAGTGGCTGGCGAAACGGCAGATTGCTGCCGATCACCTGAAGCAACTGACCGCGTTTCGGCTTACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTTTCGTCATTCACCGGCTGCATGGATGAAGGCaa                                                                                 <  2:266746/148‑1 (MQ=255)
                                                               gCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAAt                                                                                <  2:679270/148‑1 (MQ=255)
                                                                   cAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATgcgc                                                                            >  2:1751400/1‑148 (MQ=255)
                                                                   cAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATgcgc                                                                            >  2:1783583/1‑148 (MQ=255)
                                                                         ctggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGt                                                                      <  1:2390819/148‑1 (MQ=255)
                                                                         ctggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGt                                                                      >  1:694967/1‑148 (MQ=255)
                                                                           ggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGtat                                                                    >  1:2801058/1‑148 (MQ=255)
                                                                               cAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACt                                                                <  1:759105/148‑1 (MQ=255)
                                                                               cAACGGCAGATTGCTGCCGAGAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACt                                                                <  2:2279797/148‑1 (MQ=255)
                                                                                aaCGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACtt                                                               <  1:971323/148‑1 (MQ=255)
                                                                                aaCGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACtt                                                               <  2:3187665/148‑1 (MQ=255)
                                                                                aaCGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACtt                                                               <  1:2496060/148‑1 (MQ=255)
                                                                                    gCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAgcg                                                           >  2:3440792/1‑148 (MQ=255)
                                                                                    gCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAgcg                                                           >  2:1672591/1‑148 (MQ=255)
                                                                                        aTTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAc                                                       >  2:2295389/1‑148 (MQ=255)
                                                                                         ttGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAcc                                                      >  1:1274420/1‑148 (MQ=255)
                                                                                           gctgcCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAAccgc                                                    <  2:1367447/148‑1 (MQ=255)
                                                                                                   tAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGtt                                            >  2:2325615/1‑148 (MQ=255)
                                                                                                   tAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGtt                                            >  1:2884266/1‑148 (MQ=255)
                                                                                                       cTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGcc                                        <  2:3200143/148‑1 (MQ=255)
                                                                                                          aCGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAg                                     >  2:1078466/1‑148 (MQ=255)
                                                                                                             cAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCgg                                  >  1:1356599/1‑148 (MQ=255)
                                                                                                                cTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTc                               <  1:861145/148‑1 (MQ=255)
                                                                                                                 tGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTcc                              <  1:2111191/148‑1 (MQ=255)
                                                                                                                 tGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTcc                              <  2:3027361/148‑1 (MQ=255)
                                                                                                                  gACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTccc                             >  1:1930880/1‑148 (MQ=255)
                                                                                                                   aCCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCg                            >  2:2437009/1‑148 (MQ=255)
                                                                                                                    ccGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGt                           <  1:3087029/148‑1 (MQ=255)
                                                                                                                    ccGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGt                           <  1:3087166/148‑1 (MQ=255)
                                                                                                                    ccGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGt                           >  1:2100146/1‑148 (MQ=255)
                                                                                                                            cGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGtt                   <  2:2801058/148‑1 (MQ=255)
                                                                                                                              gCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTg                 <  2:609552/148‑1 (MQ=255)
                                                                                                                               cATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGt                >  2:150913/1‑148 (MQ=255)
                                                                                                                                aTACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGtt               <  1:2841565/148‑1 (MQ=255)
                                                                                                                                 tACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTa              >  2:1642975/1‑148 (MQ=255)
                                                                                                                                 tACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTa              >  1:671956/1‑148 (MQ=255)
                                                                                                                                  aCCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAc             >  1:1378757/1‑148 (MQ=255)
                                                                                                                                   ccTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAca            >  1:1420496/1‑148 (MQ=255)
                                                                                                                                    cTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAcag           >  1:3461595/1‑148 (MQ=255)
                                                                                                                                    cTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAcag           >  1:3461563/1‑148 (MQ=255)
                                                                                                                                        aGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGt       <  2:464629/148‑1 (MQ=255)
                                                                                                                                           cATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTAc    >  1:246239/1‑148 (MQ=255)
                                                                                                                                           cATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTAc    >  1:3439884/1‑148 (MQ=255)
                                                                                                                                            aTTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACg   >  2:1064933/1‑148 (MQ=255)
                                                                                                                                             ttttCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGc  <  2:905139/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGC  >  CP009273/3097118‑3097406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCA  >  CP009273/3097113‑3097407
                                                                                                                                                     |                                                                                                                                                 
CAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTT                                                                                                                                                   <  SRR19973066.3498571/150‑1 (MQ=60)
 AGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTC                                                                                                                                                  >  SRR19973066.1126006/1‑150 (MQ=60)
 AGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTC                                                                                                                                                  >  SRR19973066.1126071/1‑150 (MQ=60)
 AGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACCGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTC                                                                                                                                                  >  SRR19973066.1126810/1‑150 (MQ=60)
  GCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCA                                                                                                                                                 <  SRR19973066.2059718/150‑1 (MQ=60)
   CGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCCTTTTCAC                                                                                                                                                >  SRR19973066.2753645/1‑150 (MQ=60)
   CGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCAC                                                                                                                                                >  SRR19973066.2644236/1‑150 (MQ=60)
         CCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGAT                                                                                                                                          <  SRR19973066.1453768/150‑1 (MQ=60)
         CCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGAT                                                                                                                                          <  SRR19973066.1453544/150‑1 (MQ=60)
            AGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATT                                                                                                                                       <  SRR19973066.3208756/150‑1 (MQ=60)
             GCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTG                                                                                                                                      <  SRR19973066.2160875/150‑1 (MQ=60)
             GCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTG                                                                                                                                      <  SRR19973066.2160872/150‑1 (MQ=60)
                   TGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTA                                                                                                                                <  SRR19973066.1885167/150‑1 (MQ=60)
                      GGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGT                                                                                                                             <  SRR19973066.1834622/150‑1 (MQ=60)
                      GGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGT                                                                                                                             <  SRR19973066.1871581/150‑1 (MQ=60)
                          CGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCT                                                                                                                         <  SRR19973066.2862757/150‑1 (MQ=60)
                          CGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCT                                                                                                                         <  SRR19973066.2862810/150‑1 (MQ=60)
                            GTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTC                                                                                                                       <  SRR19973066.2920271/150‑1 (MQ=60)
                              TTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCC                                                                                                                     <  SRR19973066.2171764/150‑1 (MQ=60)
                              TTATACTGTGTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCC                                                                                                                     <  SRR19973066.3417631/150‑1 (MQ=60)
                                ATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGT                                                                                                                   <  SRR19973066.2440518/150‑1 (MQ=60)
                                 TACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTG                                                                                                                  >  SRR19973066.418358/1‑150 (MQ=60)
                                   CTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTC                                                                                                                >  SRR19973066.2589004/1‑150 (MQ=60)
                                   CTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTC                                                                                                                >  SRR19973066.2589016/1‑150 (MQ=60)
                                   CTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTC                                                                                                                <  SRR19973066.1335453/150‑1 (MQ=60)
                                   CTGTGTCCCGCAGTTTGCCGTCGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTGTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCGTCCCGTGTC                                                                                                                <  SRR19973066.2320756/150‑1 (MQ=60)
                                    TGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCG                                                                                                               <  SRR19973066.402111/150‑1 (MQ=60)
                                         CCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATT                                                                                                          >  SRR19973066.360899/1‑150 (MQ=60)
                                           CGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCA                                                                                                        <  SRR19973066.1460708/150‑1 (MQ=60)
                                            GCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCAC                                                                                                       <  SRR19973066.2008117/150‑1 (MQ=60)
                                              AGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCG                                                                                                     <  SRR19973066.720596/150‑1 (MQ=60)
                                               GTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGG                                                                                                    >  SRR19973066.1120546/1‑150 (MQ=60)
                                                 TTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCT                                                                                                  <  SRR19973066.487599/150‑1 (MQ=60)
                                                 TTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCT                                                                                                  <  SRR19973066.3018133/150‑1 (MQ=60)
                                                 TTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCT                                                                                                  <  SRR19973066.2648232/150‑1 (MQ=60)
                                                 TTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCT                                                                                                  <  SRR19973066.985399/150‑1 (MQ=60)
                                                      GACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATG                                                                                             >  SRR19973066.2767079/1‑150 (MQ=60)
                                                       ACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGG                                                                                            >  SRR19973066.644589/1‑150 (MQ=60)
                                                         GAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGAT                                                                                          >  SRR19973066.3084479/1‑150 (MQ=60)
                                                         GAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGAT                                                                                          >  SRR19973066.3083776/1‑150 (MQ=60)
                                                          AAGATAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATG                                                                                         <  SRR19973066.2682215/150‑1 (MQ=60)
                                                          AAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATG                                                                                         <  SRR19973066.1247954/150‑1 (MQ=60)
                                                            GAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAA                                                                                       >  SRR19973066.2660918/1‑150 (MQ=60)
                                                                GTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCA                                                                                   >  SRR19973066.1890837/1‑150 (MQ=60)
                                                                            GGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGT                                                                       >  SRR19973066.700093/1‑150 (MQ=60)
                                                                              CTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTAT                                                                     >  SRR19973066.2819907/1‑150 (MQ=60)
                                                                              CTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTAT                                                                     <  SRR19973066.2406908/150‑1 (MQ=60)
                                                                              CTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTAT                                                                     <  SRR19973066.9536/150‑1 (MQ=60)
                                                                                    CAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTA                                                               <  SRR19973066.764728/150‑1 (MQ=60)
                                                                                     AACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAG                                                              <  SRR19973066.2513072/150‑1 (MQ=60)
                                                                                     AACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAG                                                              <  SRR19973066.978467/150‑1 (MQ=60)
                                                                                            GATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACC                                                       >  SRR19973066.3543411/1‑150 (MQ=60)
                                                                                            GATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACC                                                       >  SRR19973066.1283624/1‑150 (MQ=60)
                                                                                                    CCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAG                                               <  SRR19973066.647682/150‑1 (MQ=60)
                                                                                                      GATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTT                                             <  SRR19973066.224757/150‑1 (MQ=60)
                                                                                                      GATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTT                                             >  SRR19973066.2903576/1‑150 (MQ=60)
                                                                                                          ACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCC                                         >  SRR19973066.2543496/1‑150 (MQ=60)
                                                                                                                CGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGG                                   >  SRR19973066.1366420/1‑150 (MQ=60)
                                                                                                                   AACTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTC                                <  SRR19973066.133633/150‑1 (MQ=60)
                                                                                                                     CTGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCC                              >  SRR19973066.1944592/1‑150 (MQ=60)
                                                                                                                     CTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCC                              <  SRR19973066.867343/150‑1 (MQ=60)
                                                                                                                     CTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCC                              <  SRR19973066.2664936/150‑1 (MQ=60)
                                                                                                                      TGACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCG                             <  SRR19973066.2125773/150‑1 (MQ=60)
                                                                                                                       GACCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGT                            >  SRR19973066.2114604/1‑150 (MQ=60)
                                                                                                                         CCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGG                          <  SRR19973066.3107793/150‑1 (MQ=60)
                                                                                                                         CCGCGTTTCGGCATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGG                          <  SRR19973066.3107654/150‑1 (MQ=60)
                                                                                                                                    CATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTA               >  SRR19973066.676878/1‑150 (MQ=60)
                                                                                                                                    CATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTA               >  SRR19973066.2790918/1‑150 (MQ=60)
                                                                                                                                     ATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAC              >  SRR19973066.1388677/1‑150 (MQ=60)
                                                                                                                                     ATACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAC              >  SRR19973066.1388730/1‑150 (MQ=60)
                                                                                                                                     ATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAC              <  SRR19973066.1604330/150‑1 (MQ=60)
                                                                                                                                     ATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTAC              <  SRR19973066.2860663/150‑1 (MQ=60)
                                                                                                                                      TACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACA             >  SRR19973066.1430663/1‑150 (MQ=60)
                                                                                                                                       ACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAG            >  SRR19973066.3484738/1‑150 (MQ=60)
                                                                                                                                       ACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAG            >  SRR19973066.3484706/1‑150 (MQ=60)
                                                                                                                                       ACCTTCAGCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAG            >  SRR19973066.3484529/1‑150 (MQ=60)
                                                                                                                                              GCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTAC     >  SRR19973066.248147/1‑150 (MQ=60)
                                                                                                                                              GCCATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTAC     >  SRR19973066.3462896/1‑150 (MQ=60)
                                                                                                                                                 ATTTTCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCA  >  SRR19973066.2238161/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
CAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCA  >  CP009273/3097113‑3097407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑