Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F434 I0 R1	1130	215.9	7031662	97.1%	6827743	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,098,900	Δ1 bp	100%	intergenic (+29/‑173)	mltC → / → nupG	membrane‑bound lytic murein transglycosylase C/nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,098,900	0	G	.	97.8%	948.9 / 12.1	183	intergenic (+29/‑173)	mltC/nupG	membrane‑bound lytic murein transglycosylase C/nucleoside transporter
Reads supporting (aligned to +/- strand): ref base G (2/2); new base . (85/94); total (87/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 195 total aligned reads displayed.

TTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACA  >  CP009273/3098753‑3099041

   acacCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAgggc                                                                                                                                            >  2:2964820/1‑147 (MQ=255)

      ccATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAgggcaaa                                                                                                                                         >  2:1106225/1‑144 (MQ=255)

       cATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCaaaa                                                                                                                                       <  1:120273/148‑1 (MQ=255)

          gACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATACCACCGCCCCCGCTAATTGCATTTACCCCCCCAGACGATGCTCA‑GGGCAAAAATg                                                                                                                                    >  1:3251337/1‑148 (MQ=255)

             gCCGGGCGATGTTTATCAGACGCTGACGACCCACCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTa                                                                                                                                 <  2:2753087/148‑1 (MQ=255)

               cGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATc                                                                                                                               <  2:2709569/148‑1 (MQ=255)

               cGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATc                                                                                                                               <  2:3069915/148‑1 (MQ=255)

                 ggCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCca                                                                                                                             <  2:2028376/148‑1 (MQ=255)

                 ggCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCca                                                                                                                             >  1:2031224/1‑148 (MQ=255)

                 ggCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCca                                                                                                                             <  2:295358/148‑1 (MQ=255)

                   cGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCcaca                                                                                                                           >  1:869675/1‑148 (MQ=255)

                    gATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAAGACCGCNCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACAt                                                                                                                          <  1:1780311/148‑1 (MQ=255)

                      tGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATca                                                                                                                        <  2:566471/148‑1 (MQ=255)

                       gTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATcac                                                                                                                       >  1:806487/1‑148 (MQ=255)

                       gTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATcac                                                                                                                       >  1:251269/1‑148 (MQ=255)

                        tttATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCCCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATcaca                                                                                                                      >  1:626093/1‑148 (MQ=255)

                            tCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAAttt                                                                                                                  >  2:2723017/1‑148 (MQ=255)

                              aGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCg                                                                                                                <  1:744733/148‑1 (MQ=255)

                                 cGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGttt                                                                                                             <  2:1224521/148‑1 (MQ=255)

                                      acgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaa                                                                                                        <  2:3003623/148‑1 (MQ=255)

                                      acgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaa                                                                                                        <  2:3003666/148‑1 (MQ=255)

                                      acgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaa                                                                                                        <  1:2967881/148‑1 (MQ=255)

                                       cgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaaa                                                                                                       <  2:2457755/148‑1 (MQ=255)

                                       cgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaaa                                                                                                       <  2:1683892/148‑1 (MQ=255)

                                       cgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAAACCTAACGCAGCCGATAATACCATTAACAGCCCCAGACGATGCTCA‑GGGCAAAAATGTTATACACATCAAAATTTCGTTTTGCaaa                                                                                                       >  2:2279860/1‑148 (MQ=255)

                                        gacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAAt                                                                                                      <  1:518686/148‑1 (MQ=255)

                                        gacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAAt                                                                                                      <  1:1248252/148‑1 (MQ=255)

                                          cccGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAAtt                                                                                                     >  1:1883769/1‑148 (MQ=255)

                                          cccGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTACATTAAACGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAAATTCGTTTTGCAAATTg                                                                                                    >  1:756526/1‑148 (MQ=255)

                                             gCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGa                                                                                                 <  2:2493425/148‑1 (MQ=255)

                                              ccATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGaa                                                                                                <  1:2091856/148‑1 (MQ=255)

                                              ccATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGaa                                                                                                <  2:3448539/148‑1 (MQ=255)

                                              ccATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGaa                                                                                                <  1:3393588/148‑1 (MQ=255)

                                              ccATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGaa                                                                                                <  1:3394539/148‑1 (MQ=255)

                                                aTCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATg                                                                                              >  2:364218/1‑148 (MQ=255)

                                                 tCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGt                                                                                             >  1:2727164/1‑148 (MQ=255)

                                                 tCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGt                                                                                             >  2:3082456/1‑148 (MQ=255)

                                                 tCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATAACGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCGGACGATGCTCA‑GGGCCAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGt                                                                                             >  1:26909/1‑148 (MQ=255)

                                                  cccTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGtt                                                                                            <  2:870227/148‑1 (MQ=255)

                                                   ccTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGttt                                                                                           >  2:632424/1‑148 (MQ=255)

                                                   agtctGCGGAATCTCTCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATACTACCGCCGCCGATAATTCCATTAACCTCCCATGACGATTCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGttt                                                                                           <  2:3365878/146‑1 (MQ=255)

                                                      ctGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCa                                                                                        <  1:668032/148‑1 (MQ=255)

                                                        gCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAat                                                                                      <  2:1342577/148‑1 (MQ=255)

                                                         cGGAATCTCGCCGTTATCTTTATAAAGTGAAGACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAatt                                                                                     <  2:3425372/148‑1 (MQ=255)

                                                           gAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAACTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAACTTGGGAATGTTTGCAatta                                                                                    <  2:79047/148‑1 (MQ=255)

                                                           gAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAattat                                                                                   <  2:3155727/148‑1 (MQ=255)

                                                           gAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAattat                                                                                   <  2:1363485/148‑1 (MQ=255)

                                                                  gCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCcac                                                                            >  2:298335/1‑148 (MQ=255)

                                                                   ccGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCGCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTTCAAATTGTGAATGTTTGCAATTATTTGCcaca                                                                           >  1:3427065/1‑148 (MQ=255)

                                                                     gTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTGGCCACAgg                                                                         >  2:2766482/1‑148 (MQ=255)

                                                                      ttATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGt                                                                        <  1:2276894/148‑1 (MQ=255)

                                                                            ttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaa                                                                  >  2:1915899/1‑148 (MQ=255)

                                                                            ttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaa                                                                  <  2:1051410/148‑1 (MQ=255)

                                                                            ttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaa                                                                  >  1:2131373/1‑148 (MQ=255)

                                                                              ataAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaaaa                                                                <  1:2335329/148‑1 (MQ=255)

                                                                              ataAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaaaa                                                                <  1:1567401/148‑1 (MQ=255)

                                                                               taAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaaaaa                                                               <  1:195004/148‑1 (MQ=255)

                                                                                aaaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAc                                                              <  1:98249/148‑1 (MQ=255)

                                                                                 aaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACACTTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAcc                                                             >  2:304391/1‑148 (MQ=255)

                                                                                 aaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAcc                                                             >  1:2449595/1‑148 (MQ=255)

                                                                                 aaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAcc                                                             >  2:1876556/1‑148 (MQ=255)

                                                                                 aaGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAAcc                                                             >  2:603406/1‑148 (MQ=255)

                                                                                   gTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAg                                                           >  1:3253619/1‑148 (MQ=255)

                                                                                   gTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAg                                                           <  2:741088/148‑1 (MQ=255)

                                                                                    tGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGt                                                          >  2:3156193/1‑148 (MQ=255)

                                                                                     gAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTc                                                         >  2:2063680/1‑148 (MQ=255)

                                                                                     gAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCAAATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTc                                                         <  2:2902589/148‑1 (MQ=255)

                                                                                        tACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCcgc                                                      >  1:2881923/1‑148 (MQ=255)

                                                                                        tACCGCGCAAAAATACTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCcgc                                                      <  2:1935133/148‑1 (MQ=255)

                                                                                           cgcgCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGaa                                                   >  1:3076110/1‑148 (MQ=255)

                                                                                              gcAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGtt                                                <  2:2982525/148‑1 (MQ=255)

                                                                                               cAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTg                                               >  1:1472375/1‑148 (MQ=255)

                                                                                               cAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTg                                               >  1:1857594/1‑148 (MQ=255)

                                                                                                  aaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATa                                            >  1:351067/1‑148 (MQ=255)

                                                                                                  aaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATa                                            >  1:936668/1‑148 (MQ=255)

                                                                                                  aaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATa                                            >  2:1518243/1‑148 (MQ=255)

                                                                                                  aaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATa                                            >  2:52273/1‑148 (MQ=255)

                                                                                                     tCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGaa                                         >  2:2203762/1‑148 (MQ=255)

                                                                                                         aCCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAACACCAGTCCGCGAAGTTGATAGAATccc                                     <  1:632424/148‑1 (MQ=255)

                                                                                                             ccgccgATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCcatca                                 <  1:1106225/148‑1 (MQ=255)

                                                                                                                ccgATAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCAtct                              >  2:3107645/1‑148 (MQ=255)

                                                                                                                   aTAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGc                           >  2:2740676/1‑148 (MQ=255)

                                                                                                                   aTAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGc                           >  1:2904952/1‑148 (MQ=255)

                                                                                                                   aTAATTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGc                           >  2:1394988/1‑148 (MQ=255)

                                                                                                                     aaTTCCATTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCAc                         >  1:3051002/1‑148 (MQ=255)

                                                                                                                          cATTAACCGCCCCTTACGATGCTCA‑GGGAAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCa                    <  1:2378442/148‑1 (MQ=255)

                                                                                                                           aTTAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCaa                   <  1:2203762/148‑1 (MQ=255)

                                                                                                                            ttAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCaaa                  <  2:267632/148‑1 (MQ=255)

                                                                                                                             tAACCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAt                 <  2:2617299/148‑1 (MQ=255)

                                                                                                                              aaCCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtg                <  1:1440129/148‑1 (MQ=255)

                                                                                                                               aCCGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtgt               <  1:2640691/148‑1 (MQ=255)

                                                                                                                                ccGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtgtg              <  1:500918/148‑1 (MQ=255)

                                                                                                                                 cGCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGc             <  1:2749977/148‑1 (MQ=255)

                                                                                                                                  gCCCCTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCt            <  1:2597230/148‑1 (MQ=255)

                                                                                                                                   ccccTGACGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCtt           <  1:2360270/148‑1 (MQ=255)

                                                                                                                                         aCGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCaa     >  1:243932/1‑148 (MQ=255)

                                                                                                                                          cGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCaaa    <  2:1531582/148‑1 (MQ=255)

                                                                                                                                          cGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCaaa    >  2:334029/1‑148 (MQ=255)

                                                                                                                                          cGATGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCaaa    <  1:1643082/148‑1 (MQ=255)

                                                                                                                                            aTGCTCA‑GGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAaca  >  1:2093389/1‑148 (MQ=255)

TTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACA  >  CP009273/3098753‑3099041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A