Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,164,049 G→T 16.8% M69I (ATG→ATT)  qseC → quorum sensing sensory histidine kinase in two‑component regulatory system with QseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,164,0490GT16.8% 868.1 / 105.5 276M69I (ATG→ATT) qseCquorum sensing sensory histidine kinase in two‑component regulatory system with QseB
Reads supporting (aligned to +/- strand):  ref base G (120/109);  new base T (27/20);  total (147/129)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

GGCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGA  >  CP009273/3163902‑3164198
                                                                                                                                                   |                                                                                                                                                     
ggCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGc                                                                                                                                                     >  1:351711/1‑150 (MQ=255)
ggCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGc                                                                                                                                                     >  2:463637/1‑150 (MQ=255)
  ccTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGcac                                                                                                                                                   >  2:2825441/1‑150 (MQ=255)
  ccTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGcac                                                                                                                                                   <  1:4476810/150‑1 (MQ=255)
    tCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAg                                                                                                                                                 <  1:626553/150‑1 (MQ=255)
     cGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGa                                                                                                                                                >  1:3379631/1‑150 (MQ=255)
      ggTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGAc                                                                                                                                               >  2:3119324/1‑150 (MQ=255)
       gTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACg                                                                                                                                              <  1:2865562/150‑1 (MQ=255)
       gTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACg                                                                                                                                              <  1:943354/150‑1 (MQ=255)
        tGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGc                                                                                                                                             >  1:4143804/1‑150 (MQ=255)
          aCCTGGCTGCTTTCCATCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCa                                                                                                                                           <  2:14920/150‑1 (MQ=255)
          aCCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCa                                                                                                                                           <  1:1660333/150‑1 (MQ=255)
          aCCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCa                                                                                                                                           <  2:1347984/150‑1 (MQ=255)
            cTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCaaa                                                                                                                                         >  2:2769366/1‑150 (MQ=255)
            cTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCaaa                                                                                                                                         >  2:2138065/1‑150 (MQ=255)
             tGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAAt                                                                                                                                        >  1:1315343/1‑150 (MQ=255)
               gctgctTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAg                                                                                                                                      <  2:498525/150‑1 (MQ=255)
               gccgctTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAg                                                                                                                                      <  2:3236936/147‑1 (MQ=255)
                 tgctTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGAt                                                                                                                                    >  2:1536465/1‑150 (MQ=255)
                 tgctTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGAt                                                                                                                                    >  1:3623775/1‑150 (MQ=255)
                           cTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACgg                                                                                                                          >  2:1083491/1‑150 (MQ=255)
                            tttGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGt                                                                                                                         <  2:1165381/150‑1 (MQ=255)
                             ttGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTc                                                                                                                        <  2:4275296/150‑1 (MQ=255)
                             ttGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTc                                                                                                                        <  1:3833094/150‑1 (MQ=255)
                              tGTCGCCTGGAAACAAACAACGGATATCGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCa                                                                                                                       <  1:1361678/150‑1 (MQ=255)
                              tGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCa                                                                                                                       >  1:80532/1‑150 (MQ=255)
                              tGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCa                                                                                                                       >  2:706691/1‑150 (MQ=255)
                                 cGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGt                                                                                                                    >  2:2796015/1‑150 (MQ=255)
                                  gCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGtt                                                                                                                   <  1:1664889/150‑1 (MQ=255)
                                      ggAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTtgatg                                                                                                               >  2:3778881/1‑150 (MQ=255)
                                      ggAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTtgatg                                                                                                               <  1:2488881/150‑1 (MQ=255)
                                        aaacaaacAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGAc                                                                                                             >  1:3426734/1‑150 (MQ=255)
                                            aaacaaCGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCCCAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATg                                                                                                         >  2:1073642/1‑150 (MQ=255)
                                            aaacaaCGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATg                                                                                                         >  2:1073297/1‑150 (MQ=255)
                                                aacGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCt                                                                                                     >  2:2565112/1‑150 (MQ=255)
                                                aacGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCt                                                                                                     <  1:4016406/150‑1 (MQ=255)
                                                  cGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGa                                                                                                   <  1:1744666/150‑1 (MQ=255)
                                                   ggATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGAc                                                                                                  >  2:1174308/1‑150 (MQ=255)
                                                   ggATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGAc                                                                                                  >  2:1751795/1‑150 (MQ=255)
                                                   ggATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGAc                                                                                                  >  1:3036214/1‑150 (MQ=255)
                                                      tAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCtt                                                                                               <  1:3276008/150‑1 (MQ=255)
                                                      tAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCtt                                                                                               >  2:4452087/1‑150 (MQ=255)
                                                       aaCGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCttt                                                                                              >  1:1617284/1‑150 (MQ=255)
                                                         cGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGc                                                                                            >  1:3754586/1‑150 (MQ=255)
                                                          gTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGcc                                                                                           >  1:4342592/1‑150 (MQ=255)
                                                           tCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCa                                                                                          <  2:1954860/150‑1 (MQ=255)
                                                             gATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATc                                                                                        >  1:1934857/1‑150 (MQ=255)
                                                             gATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATc                                                                                        >  1:664503/1‑150 (MQ=255)
                                                             gATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATc                                                                                        <  2:185949/150‑1 (MQ=255)
                                                                gAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCttt                                                                                     <  1:3543691/150‑1 (MQ=255)
                                                                gAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCttt                                                                                     <  1:2655555/150‑1 (MQ=255)
                                                                gAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCttt                                                                                     <  1:2070364/150‑1 (MQ=255)
                                                                  aTTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTAc                                                                                   >  2:2363855/1‑150 (MQ=255)
                                                                    tGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTAccc                                                                                 <  1:2113929/150‑1 (MQ=255)
                                                                        cGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCacga                                                                             >  2:3351434/1‑150 (MQ=255)
                                                                          acacCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCacgacg                                                                           <  1:575837/150‑1 (MQ=255)
                                                                              ccAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAg                                                                       <  1:333168/150‑1 (MQ=255)
                                                                              ccAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAg                                                                       <  2:4173577/150‑1 (MQ=255)
                                                                               cAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGa                                                                      >  2:3858930/1‑150 (MQ=255)
                                                                               cAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGa                                                                      <  2:3280676/150‑1 (MQ=255)
                                                                                  cTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATg                                                                   <  1:2467181/150‑1 (MQ=255)
                                                                                         tGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTa                                                            <  2:2938243/150‑1 (MQ=255)
                                                                                         tGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTa                                                            <  1:1572056/150‑1 (MQ=255)
                                                                                              gCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAatgat                                                       >  2:3618409/1‑150 (MQ=255)
                                                                                               ccAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAatgatg                                                      <  1:1016452/150‑1 (MQ=255)
                                                                                                cAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATgg                                                     <  1:3955865/150‑1 (MQ=255)
                                                                                                 aaGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGc                                                    >  1:3921054/1‑150 (MQ=255)
                                                                                                   gCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGa                                                  >  1:3421704/1‑150 (MQ=255)
                                                                                                    cGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGAt                                                 <  1:2675850/150‑1 (MQ=255)
                                                                                                       ttAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAAc                                              <  1:4253801/150‑1 (MQ=255)
                                                                                                          aGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGga                                           >  2:1275301/1‑150 (MQ=255)
                                                                                                              cGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGagaag                                       >  2:1436420/1‑150 (MQ=255)
                                                                                                               gCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGagaaga                                      >  1:3668678/1‑150 (MQ=255)
                                                                                                                   gATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGAGGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATAtt                                  >  2:4092026/1‑150 (MQ=255)
                                                                                                                   gATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATAtt                                  >  1:1206040/1‑150 (MQ=255)
                                                                                                                   gATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATAtt                                  >  2:2644345/1‑150 (MQ=255)
                                                                                                                   gATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATAtt                                  <  2:1395386/150‑1 (MQ=255)
                                                                                                                   gATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATAtt                                  >  1:2927826/1‑150 (MQ=255)
                                                                                                                     tctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTcc                                >  2:2402695/1‑150 (MQ=255)
                                                                                                                      ctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGTTCCTTAATGATGGCGATAACGGAGAAGATATTacc                               >  1:3228810/1‑150 (MQ=255)
                                                                                                                      ctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTccc                               >  1:1427207/1‑150 (MQ=255)
                                                                                                                      ctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTccc                               >  1:2800462/1‑150 (MQ=255)
                                                                                                                        cAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCta                             <  2:3306598/150‑1 (MQ=255)
                                                                                                                         aaCGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCATTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCtat                            <  2:870481/150‑1 (MQ=255)
                                                                                                                         aaCGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCtat                            >  2:1997673/1‑150 (MQ=255)
                                                                                                                          aCGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCtata                           <  2:323368/150‑1 (MQ=255)
                                                                                                                             aaaTCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCt                        >  2:508242/1‑150 (MQ=255)
                                                                                                                              aaTCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTa                       >  1:1407046/1‑150 (MQ=255)
                                                                                                                               aTCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTAt                      >  1:4058216/1‑150 (MQ=255)
                                                                                                                                tCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATc                     <  2:3949640/150‑1 (MQ=255)
                                                                                                                                 cAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCa                    <  2:4534066/150‑1 (MQ=255)
                                                                                                                                 cAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCa                    <  2:1625206/150‑1 (MQ=255)
                                                                                                                                       ggcggATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGa              >  1:2606499/1‑150 (MQ=255)
                                                                                                                                         cggATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAg            >  2:3594106/1‑150 (MQ=255)
                                                                                                                                         cggATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAg            <  2:4415502/150‑1 (MQ=255)
                                                                                                                                          ggATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAgg           <  2:3379631/150‑1 (MQ=255)
                                                                                                                                           gATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTATGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGt          >  2:550290/1‑150 (MQ=255)
                                                                                                                                                cATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGc     >  1:2196680/1‑150 (MQ=255)
                                                                                                                                                  tGGCACAGTCGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTg   <  2:1248293/150‑1 (MQ=255)
                                                                                                                                                  tGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTg   >  1:1785581/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGA  >  CP009273/3163902‑3164198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A