Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,164,049 G→T 36.4% M69I (ATG→ATT)  qseC → quorum sensing sensory histidine kinase in two‑component regulatory system with QseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,164,0490GT36.4% 357.6 / 227.0 229M69I (ATG→ATT) qseCquorum sensing sensory histidine kinase in two‑component regulatory system with QseB
Reads supporting (aligned to +/- strand):  ref base G (55/90);  new base T (46/38);  total (101/128)
Fisher's exact test for biased strand distribution p-value = 1.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

TGGCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGA  >  CP009273/3163901‑3164198
                                                                                                                                                    |                                                                                                                                                     
tGGCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATgg                                                                                                                                                      <  1:588406/150‑1 (MQ=255)
  gCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGca                                                                                                                                                    <  1:4075582/150‑1 (MQ=255)
  gCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGca                                                                                                                                                    >  1:3436159/1‑150 (MQ=255)
     tCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAg                                                                                                                                                 >  1:946042/1‑150 (MQ=255)
     tCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAg                                                                                                                                                 <  1:667984/150‑1 (MQ=255)
       ggTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGAc                                                                                                                                               >  2:198439/1‑150 (MQ=255)
       ggTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGAc                                                                                                                                               >  2:3639833/1‑150 (MQ=255)
          gACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGcc                                                                                                                                            <  1:2036940/150‑1 (MQ=255)
            ccTGGCTGCTGTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCaa                                                                                                                                          <  2:2534727/150‑1 (MQ=255)
              tGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAAt                                                                                                                                        >  1:3212842/1‑150 (MQ=255)
               ggCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATa                                                                                                                                       >  2:3029362/1‑150 (MQ=255)
                 ctgctTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGa                                                                                                                                     <  1:1279027/150‑1 (MQ=255)
                   gctTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGAtt                                                                                                                                   >  2:3775690/1‑150 (MQ=255)
                    ctTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTa                                                                                                                                  <  2:4118058/150‑1 (MQ=255)
                      ttCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTaaa                                                                                                                                <  1:2821648/150‑1 (MQ=255)
                        ccAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAac                                                                                                                              >  1:885074/1‑150 (MQ=255)
                          agatttGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAacac                                                                                                                            >  1:2930651/4‑150 (MQ=255)
                          aGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAacac                                                                                                                            <  2:2388737/150‑1 (MQ=255)
                          aGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAacac                                                                                                                            <  2:723435/150‑1 (MQ=255)
                           gCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACg                                                                                                                           <  1:1955912/150‑1 (MQ=255)
                             tttGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGt                                                                                                                         <  2:1371973/150‑1 (MQ=255)
                                gTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCACCGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCAt                                                                                                                      <  1:3896884/150‑1 (MQ=255)
                                  cGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGt                                                                                                                    >  2:799557/1‑150 (MQ=255)
                                   gCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGtt                                                                                                                   <  2:2379854/150‑1 (MQ=255)
                                    ccTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTtg                                                                                                                  <  1:1485630/150‑1 (MQ=255)
                                      tGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTtgat                                                                                                                >  2:2990912/1‑150 (MQ=255)
                                       ggAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAACGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTtgatg                                                                                                               <  1:796570/150‑1 (MQ=255)
                                        gAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTtgatga                                                                                                              <  2:861860/150‑1 (MQ=255)
                                         aaacaaacAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGAc                                                                                                             >  2:2376936/1‑150 (MQ=255)
                                          aacaaacaACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACg                                                                                                            <  2:3206099/150‑1 (MQ=255)
                                          aacaaacaACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACg                                                                                                            <  2:3656087/150‑1 (MQ=255)
                                              aacaacGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATgc                                                                                                        <  2:542426/150‑1 (MQ=255)
                                               acaacGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATgcg                                                                                                       >  2:3422054/1‑150 (MQ=255)
                                                caacGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGACAAGCGGTTAAGTACGATCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACTCCAAATAGATTAAAAAACGGTCATGTTGATGACGATgcgc                                                                                                      >  2:33955/1‑150 (MQ=255)
                                                   cGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGa                                                                                                   >  1:2667056/1‑150 (MQ=255)
                                                     gATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGAcc                                                                                                 >  1:3319807/1‑150 (MQ=255)
                                                      aTAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCt                                                                                                >  1:2677603/1‑150 (MQ=255)
                                                       tAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCtt                                                                                               >  2:2469616/1‑150 (MQ=255)
                                                       tAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCtt                                                                                               >  2:2469668/1‑150 (MQ=255)
                                                        aaCGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCttt                                                                                              <  2:44573/150‑1 (MQ=255)
                                                        aaCGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCttt                                                                                              <  1:1111306/150‑1 (MQ=255)
                                                         aCGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTg                                                                                             >  2:921038/1‑150 (MQ=255)
                                                          cGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGc                                                                                            <  2:2924550/150‑1 (MQ=255)
                                                            tCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCa                                                                                          >  1:2502833/1‑150 (MQ=255)
                                                             cGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCAt                                                                                         <  1:10596/150‑1 (MQ=255)
                                                              gATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATc                                                                                        >  2:3047843/1‑150 (MQ=255)
                                                              gATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATc                                                                                        <  2:1886226/150‑1 (MQ=255)
                                                               aTGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCt                                                                                       >  2:2784224/1‑150 (MQ=255)
                                                               aTGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCt                                                                                       <  2:3274102/150‑1 (MQ=255)
                                                                 gAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCttt                                                                                     <  1:1334013/150‑1 (MQ=255)
                                                                 gAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATATCAACGAAATCTAAGCGTATGATCGCATGGCACAGACGCCAAATAGATTAAAACACTGTCATGTTGATGACGATGCGCTGACCTTTGCCATCttt                                                                                     <  2:3811380/150‑1 (MQ=255)
                                                                   aTTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTAc                                                                                   >  2:1558882/1‑150 (MQ=255)
                                                                   aTTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTAc                                                                                   >  2:1892784/1‑150 (MQ=255)
                                                                   aTTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTAc                                                                                   >  2:4101470/1‑150 (MQ=255)
                                                                   aTTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTAc                                                                                   >  1:1208668/1‑150 (MQ=255)
                                                                    ttGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTAcc                                                                                  <  1:4160797/150‑1 (MQ=255)
                                                                     tGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTAccc                                                                                 <  1:2844287/150‑1 (MQ=255)
                                                                       ttCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCac                                                                               >  1:3740029/1‑150 (MQ=255)
                                                                                     gATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGt                                                                 >  1:1020436/1‑150 (MQ=255)
                                                                                      aTGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTc                                                                >  1:2983024/1‑150 (MQ=255)
                                                                                       tGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTcc                                                               <  2:1712230/150‑1 (MQ=255)
                                                                                          tGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTa                                                            >  2:3374075/1‑150 (MQ=255)
                                                                                            tttGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAat                                                          <  2:3061922/150‑1 (MQ=255)
                                                                                             ttGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCACCGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAatg                                                         <  1:1183829/150‑1 (MQ=255)
                                                                                              tGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAatga                                                        >  1:2724372/1‑150 (MQ=255)
                                                                                                  aaGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGc                                                    <  1:1424953/150‑1 (MQ=255)
                                                                                                   aGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCg                                                   <  2:356869/150‑1 (MQ=255)
                                                                                                     cGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGAt                                                 <  2:1497342/150‑1 (MQ=255)
                                                                                                         tAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACg                                             >  2:380018/1‑150 (MQ=255)
                                                                                                           aGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGga                                           <  1:2451768/150‑1 (MQ=255)
                                                                                                            gTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGgag                                          <  2:3193828/150‑1 (MQ=255)
                                                                                                               cGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGagaag                                       <  1:2349592/150‑1 (MQ=255)
                                                                                                                 cTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGat                                     >  1:3109708/1‑150 (MQ=255)
                                                                                                                    gATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATAtt                                  >  2:4098716/1‑150 (MQ=255)
                                                                                                                     aTCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTc                                 <  2:2756145/150‑1 (MQ=255)
                                                                                                                      tctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTcc                                <  1:3667179/150‑1 (MQ=255)
                                                                                                                       ctcAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTccc                               <  2:1823437/150‑1 (MQ=255)
                                                                                                                       ctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTccc                               >  1:307821/1‑150 (MQ=255)
                                                                                                                       ctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTccc                               >  2:213167/1‑150 (MQ=255)
                                                                                                                          accGAAATCACCGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCtat                            <  1:2317986/148‑1 (MQ=255)
                                                                                                                            cGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAg                          >  2:2062140/1‑150 (MQ=255)
                                                                                                                            cGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAg                          <  1:77589/150‑1 (MQ=255)
                                                                                                                             gAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGc                         >  1:2833761/1‑150 (MQ=255)
                                                                                                                             gAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGc                         >  2:283064/1‑150 (MQ=255)
                                                                                                                              aaaTCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCt                        >  1:3935999/1‑150 (MQ=255)
                                                                                                                               aaTCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTa                       <  2:1023968/150‑1 (MQ=255)
                                                                                                                               aaTCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTa                       <  2:4064932/150‑1 (MQ=255)
                                                                                                                                 tCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATc                     >  2:1491071/1‑150 (MQ=255)
                                                                                                                                  cAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCa                    <  2:1251224/150‑1 (MQ=255)
                                                                                                                                    aCGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAAc                  <  2:1314560/150‑1 (MQ=255)
                                                                                                                                            gATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGt          >  2:2710618/1‑150 (MQ=255)
                                                                                                                                            gATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGt          >  2:625577/1‑150 (MQ=255)
                                                                                                                                            gATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAggg          >  2:3135494/1‑149 (MQ=255)
                                                                                                                                             aTCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGtt         <  1:241751/150‑1 (MQ=255)
                                                                                                                                              tCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGttt        <  1:2376936/150‑1 (MQ=255)
                                                                                                                                               cGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGtttt       >  2:1166286/1‑150 (MQ=255)
                                                                                                                                               cGCATGGCACAGAGGCCAAAGAGATTAAAACACGTTCATGTTGATGACGATTCGCTGTCCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTTATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGtttt       <  1:1491822/150‑1 (MQ=255)
                                                                                                                                               cGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGtttt       <  2:850991/150‑1 (MQ=255)
                                                                                                                                                  aTGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCt    <  1:1756260/150‑1 (MQ=255)
                                                                                                                                                    ggCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGa  >  1:3841993/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGGCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGA  >  CP009273/3163901‑3164198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A