Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,164,049 G→T 41.2% M69I (ATG→ATT)  qseC → quorum sensing sensory histidine kinase in two‑component regulatory system with QseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,164,0490GT41.2% 296.4 / 295.2 254M69I (ATG→ATT) qseCquorum sensing sensory histidine kinase in two‑component regulatory system with QseB
Reads supporting (aligned to +/- strand):  ref base G (73/76);  new base T (59/46);  total (132/122)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

CTGGCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGA  >  CP009273/3163900‑3164198
                                                                                                                                                     |                                                                                                                                                     
     cTCGGTGACCTTGCTGCTTTCCAGCATAGTCGCCGGGAAACAAACAACTGATAACGTCTATGAATAGTTAGACACACAACTGATGCTGTTAGCCAAGCGGTTAAGTACGCTCGAACTCAACGAAATCAACGCGGCGGATCGCATGGaaca                                                                                                                                                  >  2:360206/1‑150 (MQ=255)
     cTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGcaca                                                                                                                                                  >  1:367501/1‑150 (MQ=255)
       cGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGa                                                                                                                                                <  2:1846750/150‑1 (MQ=255)
         gTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACACCGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACg                                                                                                                                              <  2:2403016/150‑1 (MQ=255)
           gACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGcc                                                                                                                                            >  1:3065130/1‑150 (MQ=255)
             ccTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCaa                                                                                                                                          <  1:3443733/150‑1 (MQ=255)
             ccTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCaa                                                                                                                                          <  1:2910005/150‑1 (MQ=255)
              cTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCAGTGCACAGACGCCaaa                                                                                                                                         >  1:1882946/1‑150 (MQ=255)
                ggCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATa                                                                                                                                       >  2:932622/1‑150 (MQ=255)
                        tCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTaaaa                                                                                                                               >  2:3790563/1‑150 (MQ=255)
                          cAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAaca                                                                                                                             <  1:4121113/150‑1 (MQ=255)
                           aGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTCCGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAacac                                                                                                                            >  2:2909951/1‑150 (MQ=255)
                            gCTTTGTCGCCTGGAAACAAACAACGGAGAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACg                                                                                                                           <  2:684437/150‑1 (MQ=255)
                             cTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACgg                                                                                                                          >  1:2569561/1‑150 (MQ=255)
                               ttGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTc                                                                                                                        <  2:3598019/150‑1 (MQ=255)
                               ttGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTc                                                                                                                        <  1:2267586/150‑1 (MQ=255)
                               ttGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTc                                                                                                                        <  1:1132010/150‑1 (MQ=255)
                                 gTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCAt                                                                                                                      >  2:3713994/1‑150 (MQ=255)
                                 gTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCAt                                                                                                                      <  2:553313/150‑1 (MQ=255)
                                   cGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGt                                                                                                                    >  1:1440431/1‑150 (MQ=255)
                                     ccTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTtg                                                                                                                  <  2:1683121/150‑1 (MQ=255)
                                     ccTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTtg                                                                                                                  <  1:1364353/150‑1 (MQ=255)
                                     ccTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTtg                                                                                                                  <  2:2080553/150‑1 (MQ=255)
                                       tGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTtgat                                                                                                                <  1:3405272/150‑1 (MQ=255)
                                            acaaacaaCGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGa                                                                                                           <  2:3187002/150‑1 (MQ=255)
                                             caaacaaCGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGAt                                                                                                          >  2:112686/1‑150 (MQ=255)
                                              aaacaaCGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATg                                                                                                         >  2:2201612/1‑150 (MQ=255)
                                               aacaacGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATgc                                                                                                        >  1:2159696/1‑150 (MQ=255)
                                                acaacGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATgcg                                                                                                       <  1:1197003/150‑1 (MQ=255)
                                                acaacGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTAGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATCTATAAAAACACGCACATGTTGATGACGAGgcg                                                                                                       >  2:4343961/1‑150 (MQ=255)
                                                  aacTGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCTGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCt                                                                                                     <  1:2621429/150‑1 (MQ=255)
                                                  aacGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCt                                                                                                     <  2:4540317/150‑1 (MQ=255)
                                                    cGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGa                                                                                                   >  1:3034954/1‑150 (MQ=255)
                                                     ggATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGAc                                                                                                  <  2:3008871/150‑1 (MQ=255)
                                                     ggATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGAc                                                                                                  >  2:2888363/1‑150 (MQ=255)
                                                      gATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGAcc                                                                                                 <  1:50242/150‑1 (MQ=255)
                                                        tAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCtt                                                                                               >  1:2988801/1‑150 (MQ=255)
                                                           cGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGc                                                                                            >  2:814984/1‑150 (MQ=255)
                                                            gTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGcc                                                                                           >  1:2027216/1‑150 (MQ=255)
                                                             tCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCa                                                                                          >  2:715044/1‑150 (MQ=255)
                                                              cGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCAt                                                                                         >  2:3949487/1‑150 (MQ=255)
                                                              cGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCAt                                                                                         <  2:80317/150‑1 (MQ=255)
                                                               gATGAATTGTTCTACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATc                                                                                        >  2:2013525/1‑150 (MQ=255)
                                                               gATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATc                                                                                        >  2:3669137/1‑150 (MQ=255)
                                                                aTGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCt                                                                                       <  1:1734361/150‑1 (MQ=255)
                                                                aTGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCt                                                                                       >  1:1724606/1‑150 (MQ=255)
                                                                 tGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCtt                                                                                      >  1:2039652/1‑150 (MQ=255)
                                                                   aaTTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTa                                                                                    >  1:3200929/1‑150 (MQ=255)
                                                                         tCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCacg                                                                              >  1:3737312/1‑150 (MQ=255)
                                                                         tCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCacg                                                                              <  1:1041416/150‑1 (MQ=255)
                                                                           gACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCacgac                                                                            >  2:4053371/1‑150 (MQ=255)
                                                                               cccAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCa                                                                        >  1:2767431/1‑150 (MQ=255)
                                                                               cccAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCa                                                                        >  1:4344540/1‑150 (MQ=255)
                                                                               cccAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCa                                                                        <  2:1870658/150‑1 (MQ=255)
                                                                                ccAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAg                                                                       >  2:142223/1‑150 (MQ=255)
                                                                                    cTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATg                                                                   >  1:2937943/1‑150 (MQ=255)
                                                                                         gCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCt                                                              >  1:4396601/1‑150 (MQ=255)
                                                                                          cTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCtt                                                             >  2:4602456/1‑150 (MQ=255)
                                                                                           tGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTa                                                            <  2:3392782/150‑1 (MQ=255)
                                                                                            gTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTaa                                                           <  2:1316796/150‑1 (MQ=255)
                                                                                             tttGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAat                                                          <  2:1043746/150‑1 (MQ=255)
                                                                                              ttGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCATTACCCACGACGGCAGAATGGTCCTTAatg                                                         >  1:4029522/1‑150 (MQ=255)
                                                                                                  cAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATgg                                                     <  1:513503/150‑1 (MQ=255)
                                                                                                   aaGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGc                                                    <  1:3819961/150‑1 (MQ=255)
                                                                                                   aaGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGc                                                    <  1:3973986/150‑1 (MQ=255)
                                                                                                   aaGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGc                                                    >  1:2077693/1‑150 (MQ=255)
                                                                                                    aGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCg                                                   <  1:3634936/150‑1 (MQ=255)
                                                                                                      cGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGAt                                                 <  1:950495/150‑1 (MQ=255)
                                                                                                       ggTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATa                                                <  1:1071918/150‑1 (MQ=255)
                                                                                                          tAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACg                                             >  1:2343587/1‑150 (MQ=255)
                                                                                                           aaGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACgg                                            >  1:4245372/1‑150 (MQ=255)
                                                                                                           aaGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACgg                                            >  1:3228402/1‑150 (MQ=255)
                                                                                                            aGTACGCTCGATCTCAAAGAAATCAACGCGGCGGATCGAATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGga                                           <  1:536760/150‑1 (MQ=255)
                                                                                                             gTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGgag                                          <  2:2293717/150‑1 (MQ=255)
                                                                                                                  cTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGat                                     <  2:2039436/150‑1 (MQ=255)
                                                                                                                   tCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACTCGGTCATGTTGATGACGATGCGCTGACCTTTGCCTTCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACTGAGAAGata                                    <  2:3136416/150‑1 (MQ=255)
                                                                                                                   tCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGata                                    <  1:429499/150‑1 (MQ=255)
                                                                                                                    cGATCTCAGCGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGatat                                   >  2:2607276/1‑150 (MQ=255)
                                                                                                                     gATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATAtt                                  >  2:4208263/1‑150 (MQ=255)
                                                                                                                     gATCTCAACGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATAtt                                  >  1:3022030/1‑150 (MQ=255)
                                                                                                                     gATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATAtt                                  >  1:4450067/1‑150 (MQ=255)
                                                                                                                     gATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCATAATGATGGCGATAACGGAGAAGATAtt                                  >  1:4450252/1‑150 (MQ=255)
                                                                                                                       tctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTcc                                >  2:2083663/1‑150 (MQ=255)
                                                                                                                       tctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTcc                                >  2:1359038/1‑150 (MQ=255)
                                                                                                                       tctcAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGAATAAAACACGGTCATGTTGATGACGATGCGCAGACATTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTcc                                >  2:4410435/1‑150 (MQ=255)
                                                                                                                           aaCGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCtat                            <  2:3798441/150‑1 (MQ=255)
                                                                                                                           aaCGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCtat                            <  2:2756502/150‑1 (MQ=255)
                                                                                                                           aaCGAAATCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCAGGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCtat                            >  2:4319985/1‑150 (MQ=255)
                                                                                                                           aaCGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCtat                            >  1:2274811/1‑150 (MQ=255)
                                                                                                                               aaaTCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCt                        <  2:3334202/150‑1 (MQ=255)
                                                                                                                                 aTCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTAt                      >  2:3433586/1‑150 (MQ=255)
                                                                                                                                 aTCAACGCGGCGGATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTAt                      >  2:3001559/1‑150 (MQ=255)
                                                                                                                                   cAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCa                    <  1:691755/150‑1 (MQ=255)
                                                                                                                                        cggcggATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACggg               >  2:2232282/1‑150 (MQ=255)
                                                                                                                                         ggcggATCGCATTGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGa              >  1:4185951/1‑150 (MQ=255)
                                                                                                                                         ggcggATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCACCGGGa              >  2:2160467/1‑150 (MQ=255)
                                                                                                                                           cggATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAg            >  1:579933/1‑150 (MQ=255)
                                                                                                                                           cggATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAg            <  2:539147/150‑1 (MQ=255)
                                                                                                                                                cGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGtttt       <  1:2698300/150‑1 (MQ=255)
                                                                                                                                                     ggCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGa  <  1:2433890/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTCGATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTCAACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGA  >  CP009273/3163900‑3164198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A