Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,089,371 A→T 37.0% E266D (GAA→GAT)  rhaR → transcriptional activator of rhaSR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,089,3710AT37.0% 286.3 / inf 294E266D (GAA→GAT) rhaRtranscriptional activator of rhaSR
Reads supporting (aligned to +/- strand):  ref base A (97/88);  new base T (72/37);  total (169/125)
Fisher's exact test for biased strand distribution p-value = 2.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 302 total aligned reads displayed.

CTGGAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCACTC  >  CP009273/4089223‑4089520
                                                                                                                                                    |                                                                                                                                                     
 tGGAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAAc                                                                                                                                                     >  1:2790069/1‑150 (MQ=255)
 tGGAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAAc                                                                                                                                                     <  1:2132487/150‑1 (MQ=255)
  ggAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAAcc                                                                                                                                                    >  1:43686/1‑150 (MQ=255)
       gACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGAt                                                                                                                                               <  2:3650086/150‑1 (MQ=255)
        aCCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTGTACCCGGGATACCGGGATg                                                                                                                                              >  2:1747196/1‑150 (MQ=255)
           atcaatcaATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACg                                                                                                                                           >  2:4577881/1‑150 (MQ=255)
              aatcaatATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGccc                                                                                                                                        >  2:4639285/1‑150 (MQ=255)
              aatcaatATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGccc                                                                                                                                        <  2:1513375/150‑1 (MQ=255)
               atcaatATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCcca                                                                                                                                       <  1:2331221/150‑1 (MQ=255)
                tcaatATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCccag                                                                                                                                      <  1:1582533/150‑1 (MQ=255)
                  aatATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCccagcc                                                                                                                                    <  1:4074134/150‑1 (MQ=255)
                  aatATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCccagcc                                                                                                                                    <  1:1709231/150‑1 (MQ=255)
                  aatATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCccagcc                                                                                                                                    >  1:4613395/1‑150 (MQ=255)
                     atCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGt                                                                                                                                 >  1:1224782/1‑150 (MQ=255)
                     atCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGt                                                                                                                                 >  2:1135539/1‑150 (MQ=255)
                       cTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTgg                                                                                                                               >  2:2608598/1‑150 (MQ=255)
                        tGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGc                                                                                                                              >  1:3077335/1‑150 (MQ=255)
                            aCAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCa                                                                                                                          <  1:423945/150‑1 (MQ=255)
                             cAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCAt                                                                                                                         <  2:5324/150‑1 (MQ=255)
                             cAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCAt                                                                                                                         <  2:1154520/150‑1 (MQ=255)
                              aGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCAtc                                                                                                                        >  1:1673967/1‑150 (MQ=255)
                                   agagTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAAt                                                                                                                   <  2:3961168/150‑1 (MQ=255)
                                   agagTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAAt                                                                                                                   >  2:2832109/1‑150 (MQ=255)
                                    gagTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAAtt                                                                                                                  <  1:2809933/150‑1 (MQ=255)
                                       tgtgtCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGc                                                                                                               >  2:224983/1‑150 (MQ=255)
                                       tgagtCATGCGCAATATCTTCTCCAGCATAGCAGCATGTTAATCATTTATATTTAGACCTAATGTTGCATTGAAGATAGTAACTATTTTTCTGTTTTGTTTACCCTGGAAAACTGGATGACGAACAGACAGAGTCTTCATCTCAATTCGc                                                                                                               >  1:1352481/4‑150 (MQ=255)
                                                     tatCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGTCGTCATCTCAATTCGCAGAAAGATTAATTc                                                                                                 <  1:4290790/150‑1 (MQ=255)
                                                     tatCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTc                                                                                                 >  2:373538/1‑150 (MQ=255)
                                                      atCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCg                                                                                                >  1:2730277/1‑150 (MQ=255)
                                                      atCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCg                                                                                                <  2:2749091/150‑1 (MQ=255)
                                                        cttctCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGcc                                                                                              <  1:1842524/150‑1 (MQ=255)
                                                          tctcCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCat                                                                                            >  2:4569416/1‑150 (MQ=255)
                                                           ctcCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatg                                                                                           >  1:2591036/1‑150 (MQ=255)
                                                             ccAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgcc                                                                                         >  1:2048302/1‑150 (MQ=255)
                                                              cAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccg                                                                                        >  1:311694/1‑150 (MQ=255)
                                                              cAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccg                                                                                        >  2:1910953/1‑150 (MQ=255)
                                                                gCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgat                                                                                      <  1:475660/150‑1 (MQ=255)
                                                                 cATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatg                                                                                     <  2:2816892/150‑1 (MQ=255)
                                                                 cATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatg                                                                                     <  2:830060/150‑1 (MQ=255)
                                                                  aTAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatgc                                                                                    >  2:1218636/1‑150 (MQ=255)
                                                                  aTAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatgc                                                                                    <  1:2092198/150‑1 (MQ=255)
                                                                    aGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatgccg                                                                                  >  2:4102756/1‑150 (MQ=255)
                                                                    aGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatgccg                                                                                  >  1:2487507/1‑150 (MQ=255)
                                                                      ccgccTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCcgac                                                                                <  2:465952/150‑1 (MQ=255)
                                                                              tAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAg                                                                        >  1:2274614/1‑150 (MQ=255)
                                                                              tAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAg                                                                        <  1:681738/150‑1 (MQ=255)
                                                                               aaTCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACACCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgc                                                                       >  2:4239291/1‑150 (MQ=255)
                                                                                aTCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgcg                                                                      <  2:2292575/150‑1 (MQ=255)
                                                                                   aGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgcggcg                                                                   >  2:1796886/1‑150 (MQ=255)
                                                                                     tGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGAc                                                                 >  1:150819/1‑150 (MQ=255)
                                                                                      gATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACa                                                                <  1:1089717/150‑1 (MQ=255)
                                                                                       atatTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACaa                                                               >  1:610356/1‑150 (MQ=255)
                                                                                       atatTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACaa                                                               <  2:1452364/150‑1 (MQ=255)
                                                                                         atTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACaata                                                             >  2:3617489/1‑150 (MQ=255)
                                                                                         atTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACaata                                                             <  1:90920/150‑1 (MQ=255)
                                                                                          tttCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACaataa                                                            <  1:3414906/150‑1 (MQ=255)
                                                                                           ttCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACaataat                                                           >  1:322828/1‑150 (MQ=255)
                                                                                            tCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATc                                                          >  1:3645080/1‑150 (MQ=255)
                                                                                                ccGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATcacca                                                      >  2:3505684/1‑150 (MQ=255)
                                                                                                 cGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATcaccac                                                     >  1:421434/1‑150 (MQ=255)
                                                                                                 cGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATcaccac                                                     >  2:3292618/1‑150 (MQ=255)
                                                                                                  gAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACcaca                                                    >  2:268541/1‑150 (MQ=255)
                                                                                                  gAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACcaca                                                    >  1:692373/1‑150 (MQ=255)
                                                                                                  gAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACcaca                                                    >  1:4056808/1‑150 (MQ=255)
                                                                                                    aTGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACcacaca                                                  >  2:3555339/1‑150 (MQ=255)
                                                                                                    aTGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACcacaca                                                  >  1:4545986/1‑150 (MQ=255)
                                                                                                    aTGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACcacaca                                                  >  2:2444185/1‑150 (MQ=255)
                                                                                                     tgtgGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACaa                                                 >  1:3156269/1‑150 (MQ=255)
                                                                                                       tgGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAAcc                                               >  2:3205353/1‑150 (MQ=255)
                                                                                                       tgGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAAcc                                               >  1:3400887/1‑150 (MQ=255)
                                                                                                         gCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGa                                             >  2:3757685/1‑150 (MQ=255)
                                                                                                          cTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAg                                            >  1:1187725/1‑150 (MQ=255)
                                                                                                          cTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAg                                            >  1:4166006/1‑150 (MQ=255)
                                                                                                            ttGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGc                                          <  1:2190390/150‑1 (MQ=255)
                                                                                                              gAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTc                                        >  2:1846973/1‑150 (MQ=255)
                                                                                                              gAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTc                                        >  1:1952730/1‑150 (MQ=255)
                                                                                                               aaGATAGTAACTATTTTTCGGTGGTGTTTACCCGTGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAATATTAATTCGCCAAGCAGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCa                                       >  1:1777962/1‑150 (MQ=255)
                                                                                                               aaGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCa                                       >  2:3841022/1‑150 (MQ=255)
                                                                                                               aaGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCa                                       <  1:2962760/150‑1 (MQ=255)
                                                                                                               aaGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCa                                       >  2:2022198/1‑150 (MQ=255)
                                                                                                                 gATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAac                                     <  2:1768145/150‑1 (MQ=255)
                                                                                                                     gTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCg                                 <  1:2457411/150‑1 (MQ=255)
                                                                                                                       aaCTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGtt                               >  2:4238290/1‑150 (MQ=255)
                                                                                                                            tttttCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCgg                          <  2:1224809/150‑1 (MQ=255)
                                                                                                                             ttttCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACCCCGTTACTGGa                         <  1:1747196/150‑1 (MQ=255)
                                                                                                                                 cGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGc                     >  1:3566508/1‑150 (MQ=255)
                                                                                                                                  ggtggtGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCg                    <  1:4431318/150‑1 (MQ=255)
                                                                                                                                    tggtgTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTc                  <  2:282673/150‑1 (MQ=255)
                                                                                                                                    tggtgTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTc                  <  1:2935072/150‑1 (MQ=255)
                                                                                                                                      gtgtTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCt                >  1:4399685/1‑150 (MQ=255)
                                                                                                                                       tgtTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTg               <  2:4416038/150‑1 (MQ=255)
                                                                                                                                        gtTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGc              >  1:4487611/1‑150 (MQ=255)
                                                                                                                                        gtTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAAGTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGc              <  1:2031829/150‑1 (MQ=255)
                                                                                                                                          ttACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCAt            >  2:2048432/1‑150 (MQ=255)
                                                                                                                                           tACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCAtg           >  2:4009872/1‑149 (MQ=255)
                                                                                                                                            aCCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTg          >  2:752614/1‑150 (MQ=255)
                                                                                                                                                 ggAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCa     >  1:3782875/1‑150 (MQ=255)
                                                                                                                                                    aaCCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCActc  <  1:1001616/150‑1 (MQ=255)
                                                                                                                                                    aaCCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCActc  <  1:3325497/150‑1 (MQ=255)
                                                                                                                                                    aaCCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCActc  <  2:2493546/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGGAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCACTC  >  CP009273/4089223‑4089520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A