Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,089,371 A→T 38.6% E266D (GAA→GAT)  rhaR → transcriptional activator of rhaSR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,089,3710AT38.6% 227.3 / inf 295E266D (GAA→GAT) rhaRtranscriptional activator of rhaSR
Reads supporting (aligned to +/- strand):  ref base A (116/64);  new base T (57/56);  total (174/121)
Fisher's exact test for biased strand distribution p-value = 2.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 299 total aligned reads displayed.

ACTGGAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCAC  >  CP009273/4089222‑4089518
                                                                                                                                                     |                                                                                                                                                   
aCTGGAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGaa                                                                                                                                                     <  1:1275305/150‑1 (MQ=255)
  tGGAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAAc                                                                                                                                                   >  1:1047837/1‑150 (MQ=255)
     aaTGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCgg                                                                                                                                                >  2:3486749/1‑150 (MQ=255)
        gACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGAt                                                                                                                                             <  1:993059/150‑1 (MQ=255)
           cATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGAc                                                                                                                                          <  2:4242832/150‑1 (MQ=255)
           cATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGAc                                                                                                                                          >  1:1972068/1‑150 (MQ=255)
              caatcaatATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGcc                                                                                                                                       <  1:2624530/150‑1 (MQ=255)
                  caatATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCccagc                                                                                                                                   >  2:2778532/1‑150 (MQ=255)
                    atatCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCccagcca                                                                                                                                 <  2:657521/150‑1 (MQ=255)
                         tGCGACAGGTCAGAGTGTGTCATGCGCAATATCGTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGc                                                                                                                            <  2:67637/150‑1 (MQ=255)
                           cGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGt                                                                                                                          <  2:2265602/150‑1 (MQ=255)
                                ggTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCAtct                                                                                                                     <  1:1120640/150‑1 (MQ=255)
                                ggTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCAtct                                                                                                                     <  2:1160465/150‑1 (MQ=255)
                                 gTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCAtctc                                                                                                                    >  2:1386637/1‑150 (MQ=255)
                                  tCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCa                                                                                                                   >  2:3409998/1‑150 (MQ=255)
                                      agTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTc                                                                                                               <  2:3107055/150‑1 (MQ=255)
                                      agTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTc                                                                                                               <  1:3159233/150‑1 (MQ=255)
                                      agTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTc                                                                                                               <  2:1963827/150‑1 (MQ=255)
                                       gtgtgtCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCg                                                                                                              <  2:4415270/150‑1 (MQ=255)
                                            tCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGaa                                                                                                         <  2:1000697/150‑1 (MQ=255)
                                              aTGCGCTATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGTTAGTATCTATTTTTCGGTGGTGTTTACCCGTGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAg                                                                                                       <  1:3129528/150‑1 (MQ=255)
                                              aTGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAg                                                                                                       >  2:2984375/1‑150 (MQ=255)
                                              aTGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAg                                                                                                       <  1:560419/150‑1 (MQ=255)
                                               tGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGa                                                                                                      <  2:2576577/150‑1 (MQ=255)
                                               tGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTGACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGa                                                                                                      <  2:1467306/150‑1 (MQ=255)
                                                 cgcAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGAtt                                                                                                    <  1:3988247/150‑1 (MQ=255)
                                                  gcAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTa                                                                                                   <  1:3587707/150‑1 (MQ=255)
                                                   cAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTaa                                                                                                  >  2:899876/1‑150 (MQ=255)
                                                   cAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTaa                                                                                                  >  2:743426/1‑150 (MQ=255)
                                                   cAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTaa                                                                                                  >  1:3788338/1‑150 (MQ=255)
                                                    aaTATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAAt                                                                                                 >  2:609425/1‑150 (MQ=255)
                                                    aaTATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAAt                                                                                                 <  1:231134/150‑1 (MQ=255)
                                                       atCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCg                                                                                              >  1:4276164/1‑150 (MQ=255)
                                                        tcttctCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGc                                                                                             >  1:1783845/1‑150 (MQ=255)
                                                        tcttctCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGATTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGc                                                                                             >  2:3376700/1‑150 (MQ=255)
                                                         cttctCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGcc                                                                                            <  1:3756217/150‑1 (MQ=255)
                                                                 gCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgat                                                                                    <  2:2095374/150‑1 (MQ=255)
                                                                    tAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatgcc                                                                                 >  2:4079977/1‑150 (MQ=255)
                                                                    tAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatgcc                                                                                 >  2:4079798/1‑150 (MQ=255)
                                                                     aGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatgccg                                                                                <  1:4417142/150‑1 (MQ=255)
                                                                     aGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCatgccgatgccg                                                                                >  1:3477495/1‑150 (MQ=255)
                                                                          ccTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGAt                                                                           >  2:3043438/1‑150 (MQ=255)
                                                                           cTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATg                                                                          >  1:2360421/1‑150 (MQ=255)
                                                                            tGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGt                                                                         <  1:279887/150‑1 (MQ=255)
                                                                            tGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGt                                                                         <  1:3650135/150‑1 (MQ=255)
                                                                            tGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGt                                                                         >  1:2039154/1‑150 (MQ=255)
                                                                            tGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGt                                                                         >  1:2938304/1‑150 (MQ=255)
                                                                            tGTTAATCAGTGATATTTCGAACGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGt                                                                         >  1:3687357/1‑150 (MQ=255)
                                                                              ttAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTa                                                                       <  1:2582883/150‑1 (MQ=255)
                                                                               tAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAg                                                                      <  1:2121050/150‑1 (MQ=255)
                                                                               tAATCAGTGATAATTCGACCGAATGTGGCTTTGAAGATAGTAACTAATTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAg                                                                      >  2:3404529/1‑150 (MQ=255)
                                                                                aaTCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgc                                                                     >  2:2041334/1‑150 (MQ=255)
                                                                                aaTCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgc                                                                     >  1:3530350/1‑150 (MQ=255)
                                                                                 aTCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgcg                                                                    <  1:1389613/150‑1 (MQ=255)
                                                                                 aTCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgcg                                                                    <  2:4349358/150‑1 (MQ=255)
                                                                                 aTCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgcg                                                                    >  2:1285879/1‑150 (MQ=255)
                                                                                 aTCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgcg                                                                    <  2:4350019/150‑1 (MQ=255)
                                                                                    aGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGACACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAgcggcg                                                                 >  2:3315437/1‑150 (MQ=255)
                                                                                       gATATTTCGACCGAATGTGGCTTTGAAGATAGTAGCTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACa                                                              >  1:3233271/1‑150 (MQ=255)
                                                                                         tatTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACaat                                                            >  1:1620598/1‑150 (MQ=255)
                                                                                            ttCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACaataat                                                         <  1:2867406/150‑1 (MQ=255)
                                                                                             tCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATc                                                        <  2:394874/150‑1 (MQ=255)
                                                                                             tCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATc                                                        <  1:1689946/150‑1 (MQ=255)
                                                                                             tCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATc                                                        >  2:2311361/1‑150 (MQ=255)
                                                                                                  cGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATcaccac                                                   >  2:2313795/1‑150 (MQ=255)
                                                                                                  cGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATcaccac                                                   >  2:1652506/1‑150 (MQ=255)
                                                                                                       gtgGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAAc                                              >  1:1533951/1‑150 (MQ=255)
                                                                                                        tgGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAAcc                                             <  2:4264533/150‑1 (MQ=255)
                                                                                                        tgGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAAcc                                             >  1:3406428/1‑150 (MQ=255)
                                                                                                          gCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGa                                           >  2:3477653/1‑150 (MQ=255)
                                                                                                          gCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGa                                           <  2:1047851/150‑1 (MQ=255)
                                                                                                           cTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAg                                          >  1:4343898/1‑150 (MQ=255)
                                                                                                            tttGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAgg                                         <  1:3581361/150‑1 (MQ=255)
                                                                                                              tGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCAATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCt                                       >  1:884899/1‑150 (MQ=255)
                                                                                                                aaGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCa                                     >  2:670164/1‑150 (MQ=255)
                                                                                                                aaGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCa                                     >  2:2496917/1‑150 (MQ=255)
                                                                                                                 aGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCaa                                    <  2:3788307/150‑1 (MQ=255)
                                                                                                                   aTAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAaca                                  >  2:813457/1‑150 (MQ=255)
                                                                                                                   aTAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAaca                                  >  1:105451/1‑150 (MQ=255)
                                                                                                                   aTAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAaca                                  >  2:1189914/1‑150 (MQ=255)
                                                                                                                    tAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAacac                                 >  2:292655/1‑150 (MQ=255)
                                                                                                                      gTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCg                               >  1:2058180/1‑150 (MQ=255)
                                                                                                                      gTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCg                               >  2:3528283/1‑150 (MQ=255)
                                                                                                                      gTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCg                               >  2:3246036/1‑150 (MQ=255)
                                                                                                                        aaCTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCgat                             >  2:1266807/1‑148 (MQ=255)
                                                                                                                          cTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCATCACCGTTAc                           >  2:1928533/1‑150 (MQ=255)
                                                                                                                            aTTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCg                         >  1:3025700/1‑150 (MQ=255)
                                                                                                                               tttCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGAc                      <  1:2825290/150‑1 (MQ=255)
                                                                                                                                ttCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACg                     >  2:1879319/1‑150 (MQ=255)
                                                                                                                                ttCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACg                     >  2:4130558/1‑150 (MQ=255)
                                                                                                                                 tCGGTGGTGTTTACCCGGGATACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACgg                    >  1:3467195/1‑150 (MQ=255)
                                                                                                                                   ggtggtGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCg                  >  1:4068858/1‑150 (MQ=255)
                                                                                                                                   ggtggtGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCg                  >  1:1815799/1‑150 (MQ=255)
                                                                                                                                     tggtgTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACTCAACCGAGGCTCAACACCGTTACCGGACGGCGTc                >  2:2616718/1‑150 (MQ=255)
                                                                                                                                        tgtTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTg             >  1:3207699/1‑150 (MQ=255)
                                                                                                                                          tttACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCa           <  1:1038245/150‑1 (MQ=255)
                                                                                                                                             aCCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTg        <  1:3344722/150‑1 (MQ=255)
                                                                                                                                                cGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTc     >  2:370994/1‑150 (MQ=255)
                                                                                                                                                  ggATACCGGGAATACGCCCACCCAGTGGCTACATCTCAATACGCAGAAAGAATAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCTAGGCTCAACAACGGTACCGGACGCAGTCCAGCAtactacca   >  2:1763571/1‑143 (MQ=255)
                                                                                                                                                   gAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAAGTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCAc  <  2:268894/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACTGGAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCAATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGGTGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAATTCGCCATGCCGATGCCGACGATGTTAGCGGCGACAATAATCACCACACAACCGAGGCTCAACACCGTTACCGGACGGCGTCCTGCATTGTTCCAC  >  CP009273/4089222‑4089518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A