Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,640,634 G→T 15.1% intergenic (+175/‑28) ydfV → / → flxA Qin prophage; uncharacterized protein/Qin prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,640,6340GT15.1% 493.0 / 58.1 173intergenic (+175/‑28)ydfV/flxAQin prophage; uncharacterized protein/Qin prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (87/58);  new base T (11/17);  total (98/75)
Fisher's exact test for biased strand distribution p-value = 5.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

ACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGAAAAGCTTGGGA  >  CP009273/1640485‑1640782
                                                                                                                                                     |                                                                                                                                                    
    gCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAg                                                                                                                                                  >  1:4559321/1‑150 (MQ=255)
    gCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAg                                                                                                                                                  >  1:4687284/1‑150 (MQ=255)
     cATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGt                                                                                                                                                 <  2:3809766/150‑1 (MQ=255)
           gCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCtttt                                                                                                                                           <  1:640629/150‑1 (MQ=255)
                 aaTTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTaaaa                                                                                                                                     >  1:3531167/1‑150 (MQ=255)
                   ttattGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAgg                                                                                                                                   <  2:2975681/150‑1 (MQ=255)
                    tattGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGga                                                                                                                                  >  2:3587309/1‑150 (MQ=255)
                     attGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGgag                                                                                                                                 >  2:2152216/1‑150 (MQ=255)
                      ttGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGgaga                                                                                                                                <  1:4576013/150‑1 (MQ=255)
                      ttGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGgaga                                                                                                                                <  1:2674914/150‑1 (MQ=255)
                        gCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGTAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGaaa                                                                                                                              <  2:4101167/150‑1 (MQ=255)
                           gAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCt                                                                                                                           >  1:3247871/1‑150 (MQ=255)
                           gAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCt                                                                                                                           <  1:1765263/150‑1 (MQ=255)
                             aCGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTAt                                                                                                                         <  1:2064648/150‑1 (MQ=255)
                             aCGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTAt                                                                                                                         >  2:2269912/1‑150 (MQ=255)
                              cGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTAtg                                                                                                                        <  1:3098562/150‑1 (MQ=255)
                                tttACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTAtgtc                                                                                                                      >  2:1194891/1‑150 (MQ=255)
                                  tACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTAtgtctg                                                                                                                    >  2:747295/1‑150 (MQ=255)
                                     cccTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTca                                                                                                                 <  2:551254/150‑1 (MQ=255)
                                     cccTGTCAACAAGCTTTACTCTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTca                                                                                                                 >  1:1904727/1‑150 (MQ=255)
                                      ccTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTcac                                                                                                                >  2:4567097/1‑150 (MQ=255)
                                        tGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACaa                                                                                                              >  1:4136150/1‑150 (MQ=255)
                                        tGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACaa                                                                                                              >  2:4110012/1‑150 (MQ=255)
                                           caacaaGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTc                                                                                                           >  2:2154480/1‑150 (MQ=255)
                                            aacaaGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCa                                                                                                          >  2:571434/1‑150 (MQ=255)
                                             acaaGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAg                                                                                                         <  2:3866934/150‑1 (MQ=255)
                                               aaGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAggg                                                                                                       >  1:3816191/1‑150 (MQ=255)
                                               aaGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAAATCAggg                                                                                                       >  2:3564818/1‑150 (MQ=255)
                                                 gCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGaa                                                                                                     <  1:2870632/150‑1 (MQ=255)
                                                        tttCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTACTGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTc                                                                                              <  2:1893078/150‑1 (MQ=255)
                                                        tttCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTc                                                                                              <  1:2661570/150‑1 (MQ=255)
                                                           cTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAAc                                                                                           >  1:4408376/1‑150 (MQ=255)
                                                            tGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAAcc                                                                                          >  1:1227411/1‑150 (MQ=255)
                                                             gAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCg                                                                                         >  2:3443521/1‑150 (MQ=255)
                                                                gcgcgcCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTa                                                                                      <  2:1521793/150‑1 (MQ=255)
                                                                    gcCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTc                                                                                  >  2:2420107/1‑150 (MQ=255)
                                                                    gcCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATACAGGGAAATACCTCAACCGTTATTTc                                                                                  >  1:1614010/1‑150 (MQ=255)
                                                                    gcCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAAGACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTGTAAAAGGAGAAATCTATGTCTGTCACACTTCAGGGAAATACCTCAACCGTTATTTc                                                                                  >  2:1840642/1‑150 (MQ=255)
                                                                        gCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAaac                                                                              <  1:2864233/150‑1 (MQ=255)
                                                                         cccGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAaaca                                                                             >  1:3244162/1‑150 (MQ=255)
                                                                            gcgAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACt                                                                          <  1:3548676/150‑1 (MQ=255)
                                                                            gcgAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACt                                                                          <  1:1000761/150‑1 (MQ=255)
                                                                             cgAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTc                                                                         >  2:3830539/1‑150 (MQ=255)
                                                                                ggAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGc                                                                      >  2:327220/1‑150 (MQ=255)
                                                                                  aaaaCAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGccc                                                                    >  1:259543/1‑150 (MQ=255)
                                                                                   aaaCAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGcccc                                                                   >  1:4444524/1‑150 (MQ=255)
                                                                                      cAATCTGAACATAAAAAAATTAATGACACAAGAAATACGATTAAATATTTTTTTGTGCATGCCGATAGTGATTTTTTAAAAGGATAAATCTTTGTCTGTCAAAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCgga                                                                <  1:592570/150‑1 (MQ=255)
                                                                                       aataataaCATCAAACAATTAAGTCCACAAGAAATACGATTAAAGTTTTTTTTGTGCATGCCGTTAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCAAAATTCAGGTAAATACCTCAACCGTTTTTTCAAACAACTCCGCCCCggaa                                                               <  1:3335386/144‑1 (MQ=255)
                                                                                       aaTCTCAACAACAAACAATTAATCACACAACAAATACTATTAAATTTTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGTAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCggaa                                                               <  1:3334422/150‑1 (MQ=255)
                                                                                        aTCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCggaag                                                              <  1:1929915/150‑1 (MQ=255)
                                                                                         tCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCggaagg                                                             >  1:1658552/1‑150 (MQ=255)
                                                                                         tCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCggaagg                                                             >  1:1658572/1‑150 (MQ=255)
                                                                                            gAACAACAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAAc                                                          <  2:2832556/150‑1 (MQ=255)
                                                                                               taTCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATc                                                       >  1:3311108/2‑150 (MQ=255)
                                                                                               caaaaaaaaaTTAATCACAAAATAAATACTATTAAAGATTTTTTTGTGCATGCCGATAGTTCTTTTTTAAAAGTAGAAATCTATGTCTGTAACAATTCAGGTAAATACCTCAACCGTTATTACAAACAACTCCGCCCCGGAAGGAACATc                                                       <  1:2480236/146‑1 (MQ=255)
                                                                                               cATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATc                                                       >  1:232491/1‑150 (MQ=255)
                                                                                                aTCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGATAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCa                                                      >  1:3277014/1‑150 (MQ=255)
                                                                                                aTCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGATAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCa                                                      >  1:2418990/1‑150 (MQ=255)
                                                                                                 tCAAACAATTAATGACACACGAAATACGATTAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAg                                                     <  1:3375309/150‑1 (MQ=255)
                                                                                                 tCAAACAATTAATGACACAAGAAATACGATTAAATATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAg                                                     <  1:3375876/150‑1 (MQ=255)
                                                                                                 tCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAg                                                     >  2:3738151/1‑150 (MQ=255)
                                                                                                 tCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAg                                                     >  2:2652638/1‑150 (MQ=255)
                                                                                                    aaCAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGaaa                                                  >  1:3006613/1‑150 (MQ=255)
                                                                                                    aaCAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGaaa                                                  >  1:372329/1‑150 (MQ=255)
                                                                                                    aaCAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGaaa                                                  >  1:1416936/1‑150 (MQ=255)
                                                                                                    aaCAAATAAAGACACAAGAAATACGATTAAAGATTTATTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGACTGTAACAATACAGGGAAATACCTCAACCGTTAATACAAACAACTCCGCCCCCGAAGGAACATAAGaaa                                                  >  1:2746887/1‑150 (MQ=255)
                                                                                                      cAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATa                                                >  1:1867796/1‑150 (MQ=255)
                                                                                                      cAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATa                                                >  1:529560/1‑150 (MQ=255)
                                                                                                        aTTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGc                                              >  1:35830/1‑150 (MQ=255)
                                                                                                         ttAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGcc                                             >  1:1162523/1‑150 (MQ=255)
                                                                                                         ttAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGcc                                             <  2:1664424/150‑1 (MQ=255)
                                                                                                         tgaaTGACACAAGAAATACGATTAAAGTTTTTTTTGTGCTTGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGcc                                             <  2:12429/148‑1 (MQ=255)
                                                                                                           aaTGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCaa                                           >  2:682358/1‑150 (MQ=255)
                                                                                                            aTGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCaaa                                          >  1:4032571/1‑150 (MQ=255)
                                                                                                               acacAAGAAATACTATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGTAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATc                                       <  2:849857/150‑1 (MQ=255)
                                                                                                               acacAAGAAATACTATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATc                                       <  2:3219842/150‑1 (MQ=255)
                                                                                                                   aaGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACaa                                   >  2:3192510/1‑150 (MQ=255)
                                                                                                                       aaTACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACa                               <  2:3127569/150‑1 (MQ=255)
                                                                                                                        aTACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACaa                              <  2:3210278/150‑1 (MQ=255)
                                                                                                                         tACGATTATAGTTTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGTATAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACaaa                             <  2:1450004/150‑1 (MQ=255)
                                                                                                                          aCGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAAt                            >  2:4003818/1‑150 (MQ=255)
                                                                                                                             aTTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCa                         <  2:1614010/150‑1 (MQ=255)
                                                                                                                              ttAATGATTTTTTTGTGCATGCCGTTTGTGATTTTTTAAAAGGAGAAATCTATGTCTGTCAAATTTCTGGTAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAg                        <  1:4708930/150‑1 (MQ=255)
                                                                                                                              ttAAAGATTTTTTTGTGCATGCCTATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAg                        <  2:4444524/150‑1 (MQ=255)
                                                                                                                                aaaGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGt                      >  2:2444029/1‑150 (MQ=255)
                                                                                                                                aaaGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGt                      >  2:4156755/1‑150 (MQ=255)
                                                                                                                                aaaGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGt                      >  2:1965117/1‑150 (MQ=255)
                                                                                                                                aaaGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGt                      >  2:2444811/1‑150 (MQ=255)
                                                                                                                                  aGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGc                    >  1:956733/1‑150 (MQ=255)
                                                                                                                                   gATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGACATAGCCAAAATCACAAGACAAATTCAGGTGct                   >  2:2869816/1‑150 (MQ=255)
                                                                                                                                   gATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGct                   >  2:3544350/1‑150 (MQ=255)
                                                                                                                                   gATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGct                   >  1:747418/1‑150 (MQ=255)
                                                                                                                                           tgtgCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGaaa           <  1:3880249/150‑1 (MQ=255)
                                                                                                                                           tgtgCATGCCGATAGTGATTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGaaa           <  1:585897/150‑1 (MQ=255)
                                                                                                                                            gtgCATGCCGATAGTGCTTTTTTAAAAGGATAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGaaaa          <  2:402273/150‑1 (MQ=255)
                                                                                                                                               cATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGAAAAGCt       >  2:2033194/1‑150 (MQ=255)
                                                                                                                                                aTGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGAAAAGCtt      <  1:2285315/150‑1 (MQ=255)
                                                                                                                                                  gCCGATATTGCTTTTTTAAAAGTAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGAAAAGCTTgg    <  2:4076987/150‑1 (MQ=255)
                                                                                                                                                    cGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGAAAAGCTTGGGa  >  2:1928311/1‑150 (MQ=255)
                                                                                                                                                    cGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGAAAAGCTTGGGa  >  2:1930793/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCCGCATGCGGCAGAAAATTATTGCCGAACGTTTACCCCTGTCAACAAGCTTTACTTTCTGAGGCGCGCCAGCCCGCGAGGAAAACAATCTGAACATCAAACAATTAATGACACAAGAAATACGATTAAAGATTTTTTTGTGCATGCCGATAGTGCTTTTTTAAAAGGAGAAATCTATGTCTGTCACAATTCAGGGAAATACCTCAACCGTTATTTCAAACAACTCCGCCCCGGAAGGAACATCAGAAATAGCCAAAATCACAAGACAAATTCAGGTGCTGACTGAAAAGCTTGGGA  >  CP009273/1640485‑1640782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A