Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,074,680 A→T 60.3% W22R (TGG→AGG)  sbmC ← DNA gyrase inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,074,6800AT60.3% 220.7 / inf 252W22R (TGG→AGG) sbmCDNA gyrase inhibitor
Reads supporting (aligned to +/- strand):  ref base A (53/47);  new base T (80/72);  total (133/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG  >  CP009273/2074533‑2074826
                                                                                                                                                   |                                                                                                                                                  
ccGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCg                                                                                                                                                  <  1:740389/150‑1 (MQ=255)
     acgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCa                                                                                                                                             <  1:1616745/150‑1 (MQ=255)
      cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCaa                                                                                                                                            >  1:4303749/1‑150 (MQ=255)
      cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCaa                                                                                                                                            >  2:3121676/1‑150 (MQ=255)
      cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCaa                                                                                                                                            >  2:752896/1‑150 (MQ=255)
        acgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACg                                                                                                                                          <  2:4038218/150‑1 (MQ=255)
          ggTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACgag                                                                                                                                        >  1:2548797/1‑150 (MQ=255)
          ggTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCAGTTCCCTCGGGCCAACgag                                                                                                                                        <  2:3599738/150‑1 (MQ=255)
              tCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATgg                                                                                                                                    >  2:3992296/1‑150 (MQ=255)
              tCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATgg                                                                                                                                    >  2:620037/1‑150 (MQ=255)
                gCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATGGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGaa                                                                                                                                  <  2:2459489/150‑1 (MQ=255)
                   gCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAcc                                                                                                                               <  2:1651336/150‑1 (MQ=255)
                    cGTAATTTTTCGGCGTGTGTTTCATCTGTATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCt                                                                                                                              <  1:478130/150‑1 (MQ=255)
                    cGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGACACCt                                                                                                                              >  2:2781402/1‑150 (MQ=255)
                       aaTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCa                                                                                                                           >  1:3054987/1‑150 (MQ=255)
                       aaTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCa                                                                                                                           >  1:2339644/1‑150 (MQ=255)
                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTa                                                                                                                     <  1:2961172/150‑1 (MQ=255)
                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTa                                                                                                                     >  2:18315/1‑150 (MQ=255)
                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTa                                                                                                                     <  1:446058/150‑1 (MQ=255)
                             tCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGACTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTa                                                                                                                     >  2:19266/1‑150 (MQ=255)
                              cggcggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTAc                                                                                                                    <  1:3788494/150‑1 (MQ=255)
                                  gggTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGttt                                                                                                                >  1:30789/1‑150 (MQ=255)
                                  gggTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGttt                                                                                                                >  2:1042069/1‑150 (MQ=255)
                                      gtTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTctct                                                                                                            <  2:3265561/150‑1 (MQ=255)
                                       tttCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCtt                                                                                                           <  1:4228445/150‑1 (MQ=255)
                                           aTCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTg                                                                                                       >  1:3939332/1‑150 (MQ=255)
                                           aTCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTg                                                                                                       <  2:2284819/150‑1 (MQ=255)
                                             cTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCt                                                                                                     >  2:3809675/1‑150 (MQ=255)
                                              tGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCtt                                                                                                    >  2:197113/1‑150 (MQ=255)
                                                   tGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCACCGGTACGTTTCTCTTCCTGCTTAAtct                                                                                               >  1:543719/1‑150 (MQ=255)
                                                   tGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAtct                                                                                               >  1:1135011/1‑150 (MQ=255)
                                                       gTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTa                                                                                           >  1:4393391/1‑150 (MQ=255)
                                                         aaTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGt                                                                                         >  2:2735768/1‑150 (MQ=255)
                                                         aaTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGt                                                                                         <  1:1733364/150‑1 (MQ=255)
                                                          aTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGtt                                                                                        >  1:2524893/1‑150 (MQ=255)
                                                          aTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGtt                                                                                        <  2:2790076/150‑1 (MQ=255)
                                                          aTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGtt                                                                                        >  2:2515672/1‑150 (MQ=255)
                                                             gACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCAt                                                                                     >  1:1224370/1‑150 (MQ=255)
                                                             gACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCAt                                                                                     <  1:1499608/150‑1 (MQ=255)
                                                               cAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGa                                                                                   >  2:610555/1‑150 (MQ=255)
                                                                aGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                                                                                  >  1:2273123/1‑150 (MQ=255)
                                                                aGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                                                                                  >  1:1303279/1‑150 (MQ=255)
                                                                  cAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGc                                                                                >  1:3143082/1‑150 (MQ=255)
                                                                  cAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGc                                                                                >  2:4654272/1‑150 (MQ=255)
                                                                   aaCCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGcc                                                                               >  2:4245241/1‑150 (MQ=255)
                                                                      ccACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCATCTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGTGCCAACGAGATGGAAACCTGAAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTcc                                                                            <  2:1491711/150‑1 (MQ=255)
                                                                       cACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                                                                           >  2:4741993/1‑150 (MQ=255)
                                                                       cACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                                                                           <  2:4186648/150‑1 (MQ=255)
                                                                        aCTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAt                                                                          <  1:2898780/150‑1 (MQ=255)
                                                                        aCTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAt                                                                          >  1:3104936/1‑150 (MQ=255)
                                                                        aCTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAt                                                                          <  2:1436020/150‑1 (MQ=255)
                                                                            cTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAt                                                                      <  1:4090704/150‑1 (MQ=255)
                                                                               cGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACt                                                                   >  2:1336994/1‑150 (MQ=255)
                                                                                    caATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGtttt                                                              <  2:4553237/150‑1 (MQ=255)
                                                                                      atatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTta                                                            <  2:167598/150‑1 (MQ=255)
                                                                                      atatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTta                                                            <  1:353677/150‑1 (MQ=255)
                                                                                        atTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtata                                                          >  1:2463039/1‑150 (MQ=255)
                                                                                          ttttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtatata                                                        <  1:3407952/150‑1 (MQ=255)
                                                                                            ttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACa                                                      <  2:1911042/150‑1 (MQ=255)
                                                                                             tGCTATCTAACCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGTTACGTTTATATTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAg                                                     >  1:789628/1‑150 (MQ=255)
                                                                                              gCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGt                                                    >  2:2907413/1‑150 (MQ=255)
                                                                                               cTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAGTGATACTGTTTTTATATACAGta                                                   >  1:3581381/1‑150 (MQ=255)
                                                                                                 aTCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGtata                                                 >  1:4693183/1‑150 (MQ=255)
                                                                                                 aTCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGtata                                                 >  2:2418646/1‑150 (MQ=255)
                                                                                                   cTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGt                                               >  2:69775/1‑150 (MQ=255)
                                                                                                   cTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGt                                               >  1:2342577/1‑150 (MQ=255)
                                                                                                    tACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGtt                                              >  2:895161/1‑150 (MQ=255)
                                                                                                     aCCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTg                                             >  2:1247655/1‑150 (MQ=255)
                                                                                                     aCCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTg                                             <  2:4477298/150‑1 (MQ=255)
                                                                                                      cccACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGc                                            <  2:3884141/150‑1 (MQ=255)
                                                                                                       ccACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCa                                           >  1:1942042/1‑150 (MQ=255)
                                                                                                        cacaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCaa                                          <  2:373736/150‑1 (MQ=255)
                                                                                                          cctcatcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCaaaa                                        <  1:3773429/148‑1 (MQ=255)
                                                                                                            tcatcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAtt                                      >  1:3600667/1‑150 (MQ=255)
                                                                                                             catcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTa                                     <  1:4654272/150‑1 (MQ=255)
                                                                                                             catcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTa                                     >  2:10224/1‑150 (MQ=255)
                                                                                                              atcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGGATAGTTGCAAAATTaa                                    >  1:1063836/1‑150 (MQ=255)
                                                                                                              atcaACTGCTCAAAGCCTTTCTTTACCGTCTGGTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTaa                                    <  2:3218513/150‑1 (MQ=255)
                                                                                                               tcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTaaa                                   >  1:2048963/1‑150 (MQ=255)
                                                                                                                  aCTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAAcc                                >  2:215947/1‑150 (MQ=255)
                                                                                                                    tGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACcac                              >  2:4715136/1‑150 (MQ=255)
                                                                                                                      cTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACaa                            <  2:3939332/150‑1 (MQ=255)
                                                                                                                        cAAAGCCTTTCTTTACCGTCTTTGCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGATTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAgg                          <  1:88396/150‑1 (MQ=255)
                                                                                                                        cAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAgg                          <  2:2618825/150‑1 (MQ=255)
                                                                                                                        cAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAgg                          >  2:1730300/1‑150 (MQ=255)
                                                                                                                          ccgCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGaa                        >  1:986312/3‑150 (MQ=255)
                                                                                                                           aGCATTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAAt                       <  2:3080463/150‑1 (MQ=255)
                                                                                                                             ccTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACATTATAGTTGCAAAATTAAAACCACAAGGAATGa                     <  1:3699648/150‑1 (MQ=255)
                                                                                                                               tttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCACAATTAAAACCACAAGGAATGAgt                   >  1:3568655/1‑150 (MQ=255)
                                                                                                                               tttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgt                   <  1:3370247/150‑1 (MQ=255)
                                                                                                                                ttctttACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgtg                  <  2:2403307/150‑1 (MQ=255)
                                                                                                                                  ctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGTGTGtt                >  2:3018612/1‑150 (MQ=255)
                                                                                                                                     tACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAt             <  1:1538767/150‑1 (MQ=255)
                                                                                                                                        cGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTAt          >  2:4354503/1‑150 (MQ=255)
                                                                                                                                        cGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTAt          >  1:3924816/1‑150 (MQ=255)
                                                                                                                                         gTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATg         <  2:2060380/150‑1 (MQ=255)
                                                                                                                                           cTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCg       <  2:1822442/150‑1 (MQ=255)
                                                                                                                                              ttCCCTCGGTCCAACGAGATGTAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGc    <  2:3347671/150‑1 (MQ=255)
                                                                                                                                                cccTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCCTTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAg  <  1:4051758/150‑1 (MQ=255)
                                                                                                                                                cccTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAg  >  2:2341026/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG  >  CP009273/2074533‑2074826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGACT  >  CP009273/2074531‑2074829
                                                                                                                                                     |                                                                                                                                                     
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCT                                                                                                                                                       >  SRR19973082.4165412/1‑150 (MQ=60)
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCT                                                                                                                                                       >  SRR19973082.4165419/1‑150 (MQ=60)
  CCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCG                                                                                                                                                     <  SRR19973082.745255/150‑1 (MQ=60)
   CGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGG                                                                                                                                                    >  SRR19973082.4733295/1‑150 (MQ=60)
    GTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGG                                                                                                                                                   >  SRR19973082.4430227/1‑150 (MQ=60)
       ACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCA                                                                                                                                                <  SRR19973082.1627424/150‑1 (MQ=60)
       ACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCA                                                                                                                                                <  SRR19973082.1627431/150‑1 (MQ=60)
        CGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAA                                                                                                                                               >  SRR19973082.4329767/1‑150 (MQ=60)
          ACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACG                                                                                                                                             >  SRR19973082.2356564/1‑150 (MQ=60)
          ACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACG                                                                                                                                             <  SRR19973082.4623308/150‑1 (MQ=60)
            GGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAG                                                                                                                                           >  SRR19973082.3907925/1‑150 (MQ=60)
            GGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAG                                                                                                                                           >  SRR19973082.2564704/1‑150 (MQ=60)
            GGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAG                                                                                                                                           >  SRR19973082.2564682/1‑150 (MQ=60)
             GTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGA                                                                                                                                          <  SRR19973082.3781669/150‑1 (MQ=60)
                      CGTAATTTTTCGGCGTGTGTTTCATCTGTATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCT                                                                                                                                 <  SRR19973082.481513/150‑1 (MQ=60)
                      CGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCT                                                                                                                                 <  SRR19973082.4059480/150‑1 (MQ=60)
                      CGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCT                                                                                                                                 <  SRR19973082.471909/150‑1 (MQ=60)
                       GTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTGTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTG                                                                                                                                >  SRR19973082.884588/1‑150 (MQ=60)
                        TAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGC                                                                                                                               <  SRR19973082.1187386/150‑1 (MQ=60)
                         AATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCA                                                                                                                              >  SRR19973082.3073722/1‑150 (MQ=60)
                         AATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCA                                                                                                                              >  SRR19973082.2354103/1‑150 (MQ=60)
                         AATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCA                                                                                                                              <  SRR19973082.2776883/150‑1 (MQ=60)
                          ATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAA                                                                                                                             >  SRR19973082.1167568/1‑150 (MQ=60)
                               TCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTA                                                                                                                        <  SRR19973082.2979467/150‑1 (MQ=60)
                               TCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTA                                                                                                                        <  SRR19973082.449142/150‑1 (MQ=60)
                                CGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTAC                                                                                                                       <  SRR19973082.3811877/150‑1 (MQ=60)
                                CGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTAC                                                                                                                       <  SRR19973082.3811885/150‑1 (MQ=60)
                                    GGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTT                                                                                                                   >  SRR19973082.31213/1‑150 (MQ=60)
                                    GGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTT                                                                                                                   >  SRR19973082.31192/1‑150 (MQ=60)
                                         TTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTT                                                                                                              <  SRR19973082.420729/150‑1 (MQ=60)
                                         TTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTT                                                                                                              <  SRR19973082.4254212/150‑1 (MQ=60)
                                         TTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTT                                                                                                              <  SRR19973082.4253966/150‑1 (MQ=60)
                                          TTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTC                                                                                                             <  SRR19973082.2319397/150‑1 (MQ=60)
                                             ATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTG                                                                                                          >  SRR19973082.3963293/1‑150 (MQ=60)
                                             ATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTG                                                                                                          >  SRR19973082.3963026/1‑150 (MQ=60)
                                              TCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGC                                                                                                         >  SRR19973082.1579092/1‑150 (MQ=60)
                                                     TGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCACCGGTACGTTTCTCTTCCTGCTTAATCT                                                                                                  >  SRR19973082.547625/1‑150 (MQ=60)
                                                     TGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCT                                                                                                  >  SRR19973082.1142563/1‑150 (MQ=60)
                                                     TGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCT                                                                                                  >  SRR19973082.547665/1‑150 (MQ=60)
                                                      GTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTC                                                                                                 <  SRR19973082.892893/150‑1 (MQ=60)
                                                       TCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCG                                                                                                >  SRR19973082.759887/1‑150 (MQ=60)
                                                         GTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTA                                                                                              >  SRR19973082.4419901/1‑150 (MQ=60)
                                                          TAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAG                                                                                             <  SRR19973082.4387261/150‑1 (MQ=60)
                                                           AATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGT                                                                                            <  SRR19973082.1744874/150‑1 (MQ=60)
                                                           AATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGT                                                                                            >  SRR19973082.1094632/1‑150 (MQ=60)
                                                            ATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCACCGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTT                                                                                           >  SRR19973082.1267787/1‑150 (MQ=60)
                                                            ATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTT                                                                                           >  SRR19973082.2540655/1‑150 (MQ=60)
                                                            ATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTT                                                                                           <  SRR19973082.3571239/150‑1 (MQ=60)
                                                            ATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCATGCTTAATCTCGTAGTT                                                                                           >  SRR19973082.2540599/1‑150 (MQ=60)
                                                               GACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCAT                                                                                        >  SRR19973082.1232710/1‑150 (MQ=60)
                                                               GACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCAT                                                                                        <  SRR19973082.1509619/150‑1 (MQ=60)
                                                                  AGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAC                                                                                     >  SRR19973082.2287344/1‑150 (MQ=60)
                                                                  AGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAC                                                                                     >  SRR19973082.4682375/1‑150 (MQ=60)
                                                                  AGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAC                                                                                     >  SRR19973082.1312284/1‑150 (MQ=60)
                                                                   GCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACG                                                                                    >  SRR19973082.3546905/1‑150 (MQ=60)
                                                                   GCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACG                                                                                    >  SRR19973082.3546724/1‑150 (MQ=60)
                                                                    CAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGC                                                                                   >  SRR19973082.842588/1‑150 (MQ=60)
                                                                    CAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGC                                                                                   >  SRR19973082.3162095/1‑150 (MQ=60)
                                                                          ACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAT                                                                             >  SRR19973082.3123858/1‑150 (MQ=60)
                                                                          ACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAT                                                                             >  SRR19973082.3311845/1‑150 (MQ=60)
                                                                          ACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAT                                                                             >  SRR19973082.3123844/1‑150 (MQ=60)
                                                                          ACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAT                                                                             <  SRR19973082.2916689/150‑1 (MQ=60)
                                                                              CTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAT                                                                         <  SRR19973082.4115776/150‑1 (MQ=60)
                                                                              CTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAT                                                                         <  SRR19973082.1715047/150‑1 (MQ=60)
                                                                                 CGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACT                                                                      <  SRR19973082.3658154/150‑1 (MQ=60)
                                                                                  GGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTG                                                                     <  SRR19973082.2064313/150‑1 (MQ=60)
                                                                                      CAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTT                                                                 <  SRR19973082.3976348/150‑1 (MQ=60)
                                                                                        ATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTA                                                               <  SRR19973082.356001/150‑1 (MQ=60)
                                                                                          ATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATA                                                             >  SRR19973082.2479347/1‑150 (MQ=60)
                                                                                          ATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATA                                                             >  SRR19973082.2478414/1‑150 (MQ=60)
                                                                                          ATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATA                                                             <  SRR19973082.3603159/150‑1 (MQ=60)
                                                                                          ATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATA                                                             <  SRR19973082.3605789/150‑1 (MQ=60)
                                                                                          ATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATA                                                             <  SRR19973082.3112715/150‑1 (MQ=60)
                                                                                            TTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATA                                                           >  SRR19973082.2991394/1‑150 (MQ=60)
                                                                                            TTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATA                                                           <  SRR19973082.385366/150‑1 (MQ=60)
                                                                                            TTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATA                                                           <  SRR19973082.3428991/150‑1 (MQ=60)
                                                                                               TGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAG                                                        >  SRR19973082.2978513/1‑150 (MQ=60)
                                                                                               TGCTATCTAACCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGTTACGTTTATATTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAG                                                        >  SRR19973082.794664/1‑150 (MQ=60)
                                                                                                GCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCTATTGATACTGTTTTTATATACAGT                                                       <  SRR19973082.4342637/150‑1 (MQ=60)
                                                                                                GCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGT                                                       <  SRR19973082.1782236/150‑1 (MQ=60)
                                                                                                 CTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTA                                                      >  SRR19973082.507857/1‑150 (MQ=60)
                                                                                                 CTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTA                                                      >  SRR19973082.2892746/1‑150 (MQ=60)
                                                                                                 CTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAGTGATACTGTTTTTATATACAGTA                                                      >  SRR19973082.3603187/1‑150 (MQ=60)
                                                                                                   ATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATA                                                    >  SRR19973082.4721465/1‑150 (MQ=60)
                                                                                                     CTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGT                                                  >  SRR19973082.2071447/1‑150 (MQ=60)
                                                                                                     CTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGT                                                  >  SRR19973082.2357041/1‑150 (MQ=60)
                                                                                                     CTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGT                                                  >  SRR19973082.2356851/1‑150 (MQ=60)
                                                                                                      TACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTT                                                 <  SRR19973082.3088054/150‑1 (MQ=60)
                                                                                                         CCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCA                                              >  SRR19973082.1954330/1‑150 (MQ=60)
                                                                                                         CCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCA                                              >  SRR19973082.1953470/1‑150 (MQ=60)
                                                                                                          CACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAA                                             <  SRR19973082.1516273/150‑1 (MQ=60)
                                                                                                           ACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAA                                            >  SRR19973082.1433799/1‑150 (MQ=60)
                                                                                                            CCTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAA                                           <  SRR19973082.3796727/150‑1 (MQ=60)
                                                                                                            CATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTCCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAA                                           <  SRR19973082.4082962/150‑1 (MQ=60)
                                                                                                              TCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATT                                         >  SRR19973082.3622638/1‑150 (MQ=60)
                                                                                                              TCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATT                                         >  SRR19973082.3622697/1‑150 (MQ=60)
                                                                                                              TCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATT                                         <  SRR19973082.4296059/150‑1 (MQ=60)
                                                                                                               CATCAACTGCTCAAAGCCTTTCTTGACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTA                                        <  SRR19973082.4682423/150‑1 (MQ=60)
                                                                                                                ATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGGATAGTTGCAAAATTAA                                       >  SRR19973082.1071140/1‑150 (MQ=60)
                                                                                                                 TCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAA                                      >  SRR19973082.2061982/1‑150 (MQ=60)
                                                                                                                    ACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTAATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACC                                   <  SRR19973082.141514/150‑1 (MQ=60)
                                                                                                                    ACAGCTCAAAGCCATTCCTTACCGCCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTAATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACC                                   <  SRR19973082.3579895/150‑1 (MQ=60)
                                                                                                                       GCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTACATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACA                                <  SRR19973082.2384290/150‑1 (MQ=60)
                                                                                                                          CAAAGCCTTTCTTTACCGTCTTTGCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGATTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGG                             <  SRR19973082.89302/150‑1 (MQ=60)
                                                                                                                            ccGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAA                           >  SRR19973082.993188/3‑150 (MQ=60)
                                                                                                                               CCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACATTATAGTTGCAAAATTAAAACCACAAGGAATGA                        <  SRR19973082.3722532/150‑1 (MQ=60)
                                                                                                                                 TTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCACAATTAAAACCACAAGGAATGAGT                      >  SRR19973082.3590293/1‑150 (MQ=60)
                                                                                                                                 TTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGT                      <  SRR19973082.3391089/150‑1 (MQ=60)
                                                                                                                                 TTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGT                      >  SRR19973082.1958465/1‑150 (MQ=60)
                                                                                                                                 TTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGT                      <  SRR19973082.3390799/150‑1 (MQ=60)
                                                                                                                                 TTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATATCGTAGTTCATGACGCCTCCATTTATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGT                      <  SRR19973082.3391111/150‑1 (MQ=60)
                                                                                                                                    CTTTACCGTCTGTTCCCACGGGAAAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTT                   <  SRR19973082.3727238/150‑1 (MQ=60)
                                                                                                                                      TTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGA                 >  SRR19973082.473662/1‑150 (MQ=60)
                                                                                                                                       TACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAT                <  SRR19973082.1548910/150‑1 (MQ=60)
                                                                                                                                          CGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTAT             >  SRR19973082.647603/1‑150 (MQ=60)
                                                                                                                                          CGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTAT             >  SRR19973082.3948739/1‑150 (MQ=60)
                                                                                                                                           GTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATG            <  SRR19973082.3064902/150‑1 (MQ=60)
                                                                                                                                                  CCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCCTTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG     <  SRR19973082.4076593/150‑1 (MQ=60)
                                                                                                                                                     TCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGACT  <  SRR19973082.3833174/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGACT  >  CP009273/2074531‑2074829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑