Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,074,680 A→T 100% W22R (TGG→AGG)  sbmC ← DNA gyrase inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,074,6800AT100.0% 747.9 / NA 225W22R (TGG→AGG) sbmCDNA gyrase inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (112/113);  total (112/113)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

TCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGC  >  CP009273/2074536‑2074824
                                                                                                                                                |                                                                                                                                                
tCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCgg                                                                                                                                               >  2:1507231/1‑148 (MQ=255)
tCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCgg                                                                                                                                               >  2:2129204/1‑148 (MQ=255)
  acgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCCACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGc                                                                                                                                             <  2:2463711/148‑1 (MQ=255)
       ggTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACg                                                                                                                                        <  1:817202/148‑1 (MQ=255)
         tgtCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACgag                                                                                                                                      <  1:3138198/148‑1 (MQ=255)
         tgtCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACgag                                                                                                                                      <  1:2646799/148‑1 (MQ=255)
         tgtCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACgag                                                                                                                                      <  1:869099/148‑1 (MQ=255)
             gCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATgg                                                                                                                                  <  2:3172586/148‑1 (MQ=255)
               aGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGaa                                                                                                                                <  1:391683/148‑1 (MQ=255)
                 cGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAc                                                                                                                              <  1:3606913/148‑1 (MQ=255)
                    aaTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTg                                                                                                                           >  1:1525878/1‑148 (MQ=255)
                      tttttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCa                                                                                                                         >  2:2783870/1‑148 (MQ=255)
                      tttttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCa                                                                                                                         >  2:548566/1‑148 (MQ=255)
                       ttttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCaa                                                                                                                        >  2:2905616/1‑148 (MQ=255)
                       ttttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCaa                                                                                                                        <  1:2447130/148‑1 (MQ=255)
                               gggTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGt                                                                                                                <  2:3797828/148‑1 (MQ=255)
                                ggTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGtt                                                                                                               >  1:1575219/1‑148 (MQ=255)
                                  tgtTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTtc                                                                                                             >  1:185504/1‑148 (MQ=255)
                                  tgtTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTtc                                                                                                             <  2:1091953/148‑1 (MQ=255)
                                   gtTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTtct                                                                                                            <  2:1615884/148‑1 (MQ=255)
                                   gtTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTtct                                                                                                            <  2:484654/148‑1 (MQ=255)
                                    tttCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTtctc                                                                                                           <  1:1515887/148‑1 (MQ=255)
                                     ttCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTctct                                                                                                          >  1:9344/1‑148 (MQ=255)
                                     ttCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTctct                                                                                                          >  2:3742772/1‑148 (MQ=255)
                                       cATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTc                                                                                                        >  1:2452778/1‑148 (MQ=255)
                                        aTCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTcc                                                                                                       <  1:128566/148‑1 (MQ=255)
                                          cTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTg                                                                                                     <  2:3016692/148‑1 (MQ=255)
                                           tGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGc                                                                                                    >  2:2668596/1‑148 (MQ=255)
                                              aTTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTa                                                                                                 >  1:3320663/1‑148 (MQ=255)
                                                tGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAt                                                                                               >  2:1770465/1‑148 (MQ=255)
                                                        tAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGt                                                                                       <  1:881021/148‑1 (MQ=255)
                                                        tAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGt                                                                                       >  1:3450917/1‑148 (MQ=255)
                                                          gACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTc                                                                                     <  2:3609/148‑1 (MQ=255)
                                                               cAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                                                                                >  2:1032908/1‑148 (MQ=255)
                                                               cAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                                                                                >  1:2521178/1‑148 (MQ=255)
                                                                 aCCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGc                                                                              >  2:199536/1‑148 (MQ=255)
                                                                  cccACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGcc                                                                             >  1:18330/1‑148 (MQ=255)
                                                                   ccACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCt                                                                            >  1:1311617/1‑148 (MQ=255)
                                                                      cTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                                                                         <  2:3554636/148‑1 (MQ=255)
                                                                       tCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAt                                                                        >  1:2287776/1‑148 (MQ=255)
                                                                         cTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTg                                                                      <  1:2358289/148‑1 (MQ=255)
                                                                            cGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATa                                                                   <  1:2181363/148‑1 (MQ=255)
                                                                            cGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATa                                                                   <  1:3328565/148‑1 (MQ=255)
                                                                            cGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATa                                                                   <  1:2477851/148‑1 (MQ=255)
                                                                             ggCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATAc                                                                  <  1:2954707/148‑1 (MQ=255)
                                                                               cacaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTg                                                                >  2:3446589/1‑148 (MQ=255)
                                                                                acaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGt                                                               <  2:3536245/148‑1 (MQ=255)
                                                                                acaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGt                                                               <  2:2420875/148‑1 (MQ=255)
                                                                                acaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGt                                                               <  2:2556438/148‑1 (MQ=255)
                                                                                acaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGt                                                               <  2:342356/148‑1 (MQ=255)
                                                                                acaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGt                                                               <  2:1483221/148‑1 (MQ=255)
                                                                                 caATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGtt                                                              >  1:186850/1‑148 (MQ=255)
                                                                                    tatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGttttt                                                           <  2:3234371/148‑1 (MQ=255)
                                                                                    tatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGttttt                                                           <  2:259229/148‑1 (MQ=255)
                                                                                    tatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGttttt                                                           >  1:2153563/1‑148 (MQ=255)
                                                                                     atTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTta                                                          <  2:2246230/148‑1 (MQ=255)
                                                                                     atTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTta                                                          >  2:753242/1‑148 (MQ=255)
                                                                                     atTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTta                                                          >  1:1016979/1‑148 (MQ=255)
                                                                                       ttttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtata                                                        >  1:1975659/1‑148 (MQ=255)
                                                                                       ttttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtata                                                        >  2:389903/1‑148 (MQ=255)
                                                                                        tttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtatat                                                       <  1:199603/148‑1 (MQ=255)
                                                                                           gCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACa                                                    <  1:310485/148‑1 (MQ=255)
                                                                                              aTCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGta                                                 >  1:2836981/1‑148 (MQ=255)
                                                                                               tCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGtat                                                >  1:2776031/1‑148 (MQ=255)
                                                                                                cTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGtata                                               <  2:101828/148‑1 (MQ=255)
                                                                                                 tACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAg                                              <  2:3085199/148‑1 (MQ=255)
                                                                                                     cacaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGGTACTGTTTTTATATACAGTATAGTTGc                                          >  1:45929/1‑148 (MQ=255)
                                                                                                     cacaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGc                                          >  1:1369268/1‑148 (MQ=255)
                                                                                                      acaTCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCa                                         >  1:779424/1‑148 (MQ=255)
                                                                                                         tcatcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCaaaa                                      >  1:559112/1‑148 (MQ=255)
                                                                                                         tcatcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCaaaa                                      >  2:3679563/1‑148 (MQ=255)
                                                                                                           atcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAtt                                    >  2:1997233/1‑148 (MQ=255)
                                                                                                           atcaACTGCTCAAAGCCTTTCATTAACGTCTGTTCCCTCTGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGATCAAGACGCCTCCATTGATACTGTTATTATATACAGTATAGATGCAAAAtt                                    >  2:635151/1‑148 (MQ=255)
                                                                                                           atcaACTGCTCAAAGCCATTCTTGACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTGTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTGATATACAGTATAGTTGCAAAAtt                                    <  2:1561726/148‑1 (MQ=255)
                                                                                                               aCTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACTTTTCTCTTCCTGCTTAAGCTCGTCGTTCATGACGCCTCCATTGATACTGTTTTTATATAAAGTATAGTTGCAAAATTaaaa                                >  1:3666204/1‑148 (MQ=255)
                                                                                                               aCTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTaaaa                                <  2:1939846/148‑1 (MQ=255)
                                                                                                                cTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAAc                               >  2:3448543/1‑148 (MQ=255)
                                                                                                                 tGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGAAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAAcc                              >  1:1768693/1‑148 (MQ=255)
                                                                                                                   cTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACcac                            >  2:80655/1‑148 (MQ=255)
                                                                                                                   cTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACcac                            >  2:1028385/1‑148 (MQ=255)
                                                                                                                    tCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGTTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACcaca                           <  1:2387153/148‑1 (MQ=255)
                                                                                                                    tCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACcaca                           <  2:118220/148‑1 (MQ=255)
                                                                                                                     cAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACaa                          >  1:1678895/1‑148 (MQ=255)
                                                                                                                      aaaGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAg                         >  1:2742860/1‑148 (MQ=255)
                                                                                                                         gCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGac                      >  2:3605317/1‑147 (MQ=255)
                                                                                                                         gCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGaa                      <  2:1842711/148‑1 (MQ=255)
                                                                                                                          ccTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAAt                     <  2:207117/148‑1 (MQ=255)
                                                                                                                            tttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGa                   >  1:1656609/1‑148 (MQ=255)
                                                                                                                             ttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAg                  >  2:2288132/1‑148 (MQ=255)
                                                                                                                               ctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgtg                <  1:3742772/148‑1 (MQ=255)
                                                                                                                                   aCCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGa            <  1:762844/148‑1 (MQ=255)
                                                                                                                                    ccGTCTGTGCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGATTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAt           <  1:3517831/148‑1 (MQ=255)
                                                                                                                                     cGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAtt          <  2:2742860/148‑1 (MQ=255)
                                                                                                                                      gTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTa         <  2:18330/148‑1 (MQ=255)
                                                                                                                                        cTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATg       >  1:2547737/1‑148 (MQ=255)
                                                                                                                                        cTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATg       >  1:1636752/1‑148 (MQ=255)
                                                                                                                                         tGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGc      >  1:1073402/1‑148 (MQ=255)
                                                                                                                                          gTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCg     <  1:2668596/148‑1 (MQ=255)
                                                                                                                                            tCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAg   >  1:1674407/1‑148 (MQ=255)
                                                                                                                                             cccTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGc  <  2:1904214/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGC  >  CP009273/2074536‑2074824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGA  >  CP009273/2074532‑2074827
                                                                                                                                                    |                                                                                                                                                   
ACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTC                                                                                                                                                    <  SRR19973069.2702938/150‑1 (MQ=60)
      ACGACGGTGTCGCAGCGTAATTTTTCGGGGGGTGTTTCATCTGGATTGTCTTAATAGACAGCAACCCACTCCTTCGGCAAAATATTTTTGCTATCTACCCAAATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCA                                                                                                                                              <  SRR19973069.169192/150‑1 (MQ=60)
      ACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCA                                                                                                                                              <  SRR19973069.1484738/150‑1 (MQ=60)
           GGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAG                                                                                                                                         <  SRR19973069.822970/150‑1 (MQ=60)
           GGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAG                                                                                                                                         <  SRR19973069.823004/150‑1 (MQ=60)
             TGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGAT                                                                                                                                       <  SRR19973069.3158755/150‑1 (MQ=60)
             TGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGAT                                                                                                                                       <  SRR19973069.2664529/150‑1 (MQ=60)
             TGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGAT                                                                                                                                       <  SRR19973069.875153/150‑1 (MQ=60)
             TGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGAT                                                                                                                                       <  SRR19973069.2801212/150‑1 (MQ=60)
                CGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGA                                                                                                                                    >  SRR19973069.2223435/1‑150 (MQ=60)
                CGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGA                                                                                                                                    >  SRR19973069.1298199/1‑150 (MQ=60)
                   AGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAC                                                                                                                                 <  SRR19973069.393920/150‑1 (MQ=60)
                   AGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAC                                                                                                                                 <  SRR19973069.394561/150‑1 (MQ=60)
                     CGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCT                                                                                                                               <  SRR19973069.3630461/150‑1 (MQ=60)
                     CGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCT                                                                                                                               >  SRR19973069.2757930/1‑150 (MQ=60)
                     CGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCT                                                                                                                               <  SRR19973069.3632552/150‑1 (MQ=60)
                      GTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTG                                                                                                                              <  SRR19973069.3316744/150‑1 (MQ=60)
                      GTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTG                                                                                                                              >  SRR19973069.1536384/1‑150 (MQ=60)
                      GTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTG                                                                                                                              >  SRR19973069.1536446/1‑150 (MQ=60)
                           TTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACG                                                                                                                         <  SRR19973069.2463601/150‑1 (MQ=60)
                                  CGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTT                                                                                                                  >  SRR19973069.1585924/1‑150 (MQ=60)
                                    GGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTC                                                                                                                >  SRR19973069.187036/1‑150 (MQ=60)
                                     GTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCT                                                                                                               <  SRR19973069.1129065/150‑1 (MQ=60)
                                       GTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCT                                                                                                             <  SRR19973069.2212426/150‑1 (MQ=60)
                                       GTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCT                                                                                                             >  SRR19973069.9484/1‑150 (MQ=60)
                                        TTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTT                                                                                                            <  SRR19973069.1526354/150‑1 (MQ=60)
                                        TTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTT                                                                                                            <  SRR19973069.552407/150‑1 (MQ=60)
                                        TTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTT                                                                                                            <  SRR19973069.1526361/150‑1 (MQ=60)
                                         TTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTC                                                                                                           >  SRR19973069.2469274/1‑150 (MQ=60)
                                         TTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTC                                                                                                           >  SRR19973069.2470500/1‑150 (MQ=60)
                                           CATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCT                                                                                                         >  SRR19973069.1953199/1‑150 (MQ=60)
                                           CATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCT                                                                                                         <  SRR19973069.1847654/150‑1 (MQ=60)
                                            ATCTGGATTGTCGTAATAGACAGCAGCCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTG                                                                                                        >  SRR19973069.44212/1‑150 (MQ=60)
                                            ATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTG                                                                                                        <  SRR19973069.129770/150‑1 (MQ=60)
                                            ATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTG                                                                                                        >  SRR19973069.44183/1‑150 (MQ=60)
                                            ATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTG                                                                                                        >  SRR19973069.1351958/1‑150 (MQ=60)
                                              CTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCT                                                                                                      >  SRR19973069.2466667/1‑150 (MQ=60)
                                               TGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTT                                                                                                     >  SRR19973069.2672584/1‑150 (MQ=60)
                                                GGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTA                                                                                                    >  SRR19973069.3342434/1‑150 (MQ=60)
                                                 GATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAA                                                                                                   >  SRR19973069.3169391/1‑150 (MQ=60)
                                                    TGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCT                                                                                                >  SRR19973069.3563956/1‑150 (MQ=60)
                                                    TGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCT                                                                                                <  SRR19973069.3600273/150‑1 (MQ=60)
                                                        GTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTA                                                                                            >  SRR19973069.2157941/1‑150 (MQ=60)
                                                          AATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGT                                                                                          >  SRR19973069.3473292/1‑150 (MQ=60)
                                                            TAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTC                                                                                        <  SRR19973069.887128/150‑1 (MQ=60)
                                                                 AGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAC                                                                                   <  SRR19973069.3487843/150‑1 (MQ=60)
                                                                 AGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAC                                                                                   >  SRR19973069.2537940/1‑150 (MQ=60)
                                                                    AACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCC                                                                                >  SRR19973069.18588/1‑150 (MQ=60)
                                                                    AACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCC                                                                                >  SRR19973069.18899/1‑150 (MQ=60)
                                                                     ACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCT                                                                               >  SRR19973069.1320591/1‑150 (MQ=60)
                                                                        CACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCA                                                                            >  SRR19973069.2499667/1‑150 (MQ=60)
                                                                         ACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAT                                                                           >  SRR19973069.2303323/1‑150 (MQ=60)
                                                                             CTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAT                                                                       <  SRR19973069.2374266/150‑1 (MQ=60)
                                                                             CTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAT                                                                       <  SRR19973069.2374312/150‑1 (MQ=60)
                                                                             CTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAT                                                                       <  SRR19973069.2374442/150‑1 (MQ=60)
                                                                                CGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACT                                                                    <  SRR19973069.2196139/150‑1 (MQ=60)
                                                                                CGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACT                                                                    >  SRR19973069.3078787/1‑150 (MQ=60)
                                                                                CGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACT                                                                    <  SRR19973069.3349489/150‑1 (MQ=60)
                                                                                CGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACT                                                                    <  SRR19973069.3350378/150‑1 (MQ=60)
                                                                                CGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACT                                                                    <  SRR19973069.2494452/150‑1 (MQ=60)
                                                                                CGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACT                                                                    <  SRR19973069.2196232/150‑1 (MQ=60)
                                                                                 GGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTG                                                                   <  SRR19973069.2974320/150‑1 (MQ=60)
                                                                                  GCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGT                                                                  <  SRR19973069.1040068/150‑1 (MQ=60)
                                                                                   CACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTT                                                                 >  SRR19973069.188388/1‑150 (MQ=60)
                                                                                     CAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTT                                                               <  SRR19973069.775610/150‑1 (MQ=60)
                                                                                     CAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTT                                                               >  SRR19973069.795973/1‑150 (MQ=60)
                                                                                     CAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTT                                                               >  SRR19973069.2425394/1‑150 (MQ=60)
                                                                                      AATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTT                                                              >  SRR19973069.879010/1‑150 (MQ=60)
                                                                                      AATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTT                                                              >  SRR19973069.2168313/1‑150 (MQ=60)
                                                                                       ATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTA                                                             >  SRR19973069.1023998/1‑150 (MQ=60)
                                                                                         ATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATA                                                           >  SRR19973069.1989396/1‑150 (MQ=60)
                                                                                            TTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATAC                                                        <  SRR19973069.201203/150‑1 (MQ=60)
                                                                                            TTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATAC                                                        <  SRR19973069.201136/150‑1 (MQ=60)
                                                                                               GCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGT                                                     <  SRR19973069.312563/150‑1 (MQ=60)
                                                                                                CTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTA                                                    >  SRR19973069.929416/1‑150 (MQ=60)
                                                                                                CTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTA                                                    >  SRR19973069.2855710/1‑150 (MQ=60)
                                                                                                 TATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTAT                                                   <  SRR19973069.2463647/150‑1 (MQ=60)
                                                                                                 TATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTAT                                                   >  SRR19973069.2794520/1‑150 (MQ=60)
                                                                                                 TATCGACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTAT                                                   <  SRR19973069.1326562/150‑1 (MQ=60)
                                                                                                  ATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATA                                                  >  SRR19973069.2584713/1‑150 (MQ=60)
                                                                                                  ATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATA                                                  >  SRR19973069.1704518/1‑150 (MQ=60)
                                                                                                  ATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATA                                                  <  SRR19973069.1900153/150‑1 (MQ=60)
                                                                                                   TCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAG                                                 <  SRR19973069.2888720/150‑1 (MQ=60)
                                                                                                   TCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAG                                                 <  SRR19973069.964406/150‑1 (MQ=60)
                                                                                                    CTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGT                                                >  SRR19973069.660742/1‑150 (MQ=60)
                                                                                                    CTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGA                                                >  SRR19973069.2596278/1‑150 (MQ=60)
                                                                                                      ACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTG                                              >  SRR19973069.947873/1‑150 (MQ=60)
                                                                                                       CCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGGTACTGTTTTTATATACAGTATAGTTGC                                             >  SRR19973069.46516/1‑150 (MQ=60)
                                                                                                       CCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATAGAGTATAGTTGC                                             >  SRR19973069.1378795/1‑150 (MQ=60)
                                                                                                        CCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCA                                            >  SRR19973069.785734/1‑150 (MQ=60)
                                                                                                        CCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCA                                            >  SRR19973069.784946/1‑150 (MQ=60)
                                                                                                           CATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAA                                         >  SRR19973069.563005/1‑150 (MQ=60)
                                                                                                                 CAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACTTTTCTCTTCCTGCTTAAGCTCGTCGTTCATGACGCCTCCATTGATACTGTTTTTATATAAAGTATAGTTGCAAAATTAAAA                                   >  SRR19973069.3690198/1‑150 (MQ=60)
                                                                                                                  AACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAAC                                  <  SRR19973069.3716615/150‑1 (MQ=60)
                                                                                                                   ACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGAAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACC                                 >  SRR19973069.1781157/1‑150 (MQ=60)
                                                                                                                      GCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACA                              <  SRR19973069.2802398/150‑1 (MQ=60)
                                                                                                                       CTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAA                             >  SRR19973069.1691112/1‑150 (MQ=60)
                                                                                                                       CTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAA                             >  SRR19973069.1690599/1‑150 (MQ=60)
                                                                                                                        TCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGTTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAG                            <  SRR19973069.2403335/150‑1 (MQ=60)
                                                                                                                        TCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAG                            <  SRR19973069.2559859/150‑1 (MQ=60)
                                                                                                                        TCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAG                            >  SRR19973069.2761191/1‑150 (MQ=60)
                                                                                                                              CCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGA                      >  SRR19973069.1668981/1‑150 (MQ=60)
                                                                                                                              CCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGA                      >  SRR19973069.1668074/1‑150 (MQ=60)
                                                                                                                                   CTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTT                 <  SRR19973069.2794207/150‑1 (MQ=60)
                                                                                                                                   CTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTT                 <  SRR19973069.3767163/150‑1 (MQ=60)
                                                                                                                                   CTTTACCGACTGTTCCCTCGGGCCAACGAGATGTAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTATTTCATGACGCCTCCATTGATAATGTTTTTATATAAAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTT                 <  SRR19973069.1630283/150‑1 (MQ=60)
                                                                                                                                    TTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTG                <  SRR19973069.1782937/150‑1 (MQ=60)
                                                                                                                                       ACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATT             <  SRR19973069.768230/150‑1 (MQ=60)
                                                                                                                                        CCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGTTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTA            <  SRR19973069.1640182/150‑1 (MQ=60)
                                                                                                                                        CCGTCTGTGCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGATTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTA            <  SRR19973069.3540531/150‑1 (MQ=60)
                                                                                                                                          GTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTGTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATG          <  SRR19973069.3365580/150‑1 (MQ=60)
                                                                                                                                          GTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATG          >  SRR19973069.1647965/1‑150 (MQ=60)
                                                                                                                                          GTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATG          >  SRR19973069.2564576/1‑150 (MQ=60)
                                                                                                                                          GTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATG          >  SRR19973069.1648105/1‑150 (MQ=60)
                                                                                                                                           TCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGC         >  SRR19973069.1080887/1‑150 (MQ=60)
                                                                                                                                           TCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGC         >  SRR19973069.3157599/1‑150 (MQ=60)
                                                                                                                                              GTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAG      >  SRR19973069.1686061/1‑150 (MQ=60)
                                                                                                                                              GTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAG      <  SRR19973069.2686493/150‑1 (MQ=60)
                                                                                                                                                TCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCA    >  SRR19973069.388465/1‑150 (MQ=60)
                                                                                                                                                TCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCA    >  SRR19973069.3344561/1‑150 (MQ=60)
                                                                                                                                                 CCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG   >  SRR19973069.1498968/1‑150 (MQ=60)
                                                                                                                                                 CCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG   >  SRR19973069.3428128/1‑150 (MQ=60)
                                                                                                                                                 CCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGAGAGCAG   >  SRR19973069.3428092/1‑150 (MQ=60)
                                                                                                                                                  CCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACAACAAGGAATGAGTGTTGATTATGCGAGCAGA  >  SRR19973069.3301407/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGA  >  CP009273/2074532‑2074827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑