Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,074,680 A→T 88.7% W22R (TGG→AGG)  sbmC ← DNA gyrase inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,074,6800AT88.7% 727.5 / 68.2 229W22R (TGG→AGG) sbmCDNA gyrase inhibitor
Reads supporting (aligned to +/- strand):  ref base A (17/9);  new base T (101/102);  total (118/111)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 231 total aligned reads displayed.

CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG  >  CP009273/2074531‑2074826
                                                                                                                                                     |                                                                                                                                                  
cACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCa                                                                                                                                                    <  1:612383/150‑1 (MQ=255)
   cGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCgg                                                                                                                                                 >  2:3992366/1‑150 (MQ=255)
      cACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGcc                                                                                                                                              >  1:639425/1‑150 (MQ=255)
        cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCC‑TCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAAc                                                                                                                                           <  1:4531018/150‑1 (MQ=255)
         gacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAAc                                                                                                                                           >  1:4502013/1‑150 (MQ=255)
                 cGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGa                                                                                                                                   >  2:3437961/1‑150 (MQ=255)
                  gCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGaa                                                                                                                                  <  1:527166/150‑1 (MQ=255)
                    aGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAc                                                                                                                                >  2:3359347/1‑150 (MQ=255)
                    aGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAc                                                                                                                                <  1:2681239/150‑1 (MQ=255)
                    aGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAc                                                                                                                                >  1:2906691/1‑150 (MQ=255)
                    aGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTACTTTACCGTCTGTTCCCTCGGGCCACCGAGATGGacac                                                                                                                                >  2:3783988/1‑150 (MQ=255)
                      cGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAccc                                                                                                                              >  2:3833935/1‑149 (MQ=255)
                       gTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTg                                                                                                                             <  1:419470/150‑1 (MQ=255)
                         aaTTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCa                                                                                                                           <  1:1250937/150‑1 (MQ=255)
                            ttttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACg                                                                                                                        <  1:2272735/150‑1 (MQ=255)
                             tttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACgg                                                                                                                       <  2:312475/150‑1 (MQ=255)
                                 ggcggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACg                                                                                                                   <  2:1731142/150‑1 (MQ=255)
                                      gtgtTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTtct                                                                                                              <  1:1282288/150‑1 (MQ=255)
                                         tttCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCtt                                                                                                           <  1:1377577/150‑1 (MQ=255)
                                         tttCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGATCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGATTCTCtt                                                                                                           >  2:2808426/1‑150 (MQ=255)
                                          ttCATCTGTATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTc                                                                                                          <  2:3177164/150‑1 (MQ=255)
                                          ttCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTc                                                                                                          >  1:832067/1‑150 (MQ=255)
                                           tCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTcc                                                                                                         <  1:3152623/150‑1 (MQ=255)
                                           tCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTcc                                                                                                         <  1:4259486/150‑1 (MQ=255)
                                            cATCTGGATTGTCGTAATAGACAGCAACCCACCCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCt                                                                                                        <  1:1891749/150‑1 (MQ=255)
                                               cTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCt                                                                                                     >  2:4374063/1‑150 (MQ=255)
                                                tGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTGTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCtt                                                                                                    <  2:2692437/150‑1 (MQ=255)
                                                 ggATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTa                                                                                                   >  2:248607/1‑150 (MQ=255)
                                                 ggATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTa                                                                                                   <  1:3586468/150‑1 (MQ=255)
                                                  gATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTaa                                                                                                  <  1:2525051/150‑1 (MQ=255)
                                                  gATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTaa                                                                                                  >  1:3637545/1‑150 (MQ=255)
                                                   aTTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAt                                                                                                 >  2:4417708/1‑150 (MQ=255)
                                                    ttGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAtc                                                                                                <  2:1187632/150‑1 (MQ=255)
                                                     tGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAtct                                                                                               <  1:1744116/150‑1 (MQ=255)
                                                      gTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAAtctc                                                                                              >  2:1059764/1‑150 (MQ=255)
                                                          tAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAg                                                                                          <  1:1794321/150‑1 (MQ=255)
                                                            aTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGtt                                                                                        >  2:2765236/1‑150 (MQ=255)
                                                                  aGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                                                                                  <  2:2873956/150‑1 (MQ=255)
                                                                  aGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGAc                                                                                  >  2:2436992/1‑150 (MQ=255)
                                                                     aaCCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGcc                                                                               <  2:151021/150‑1 (MQ=255)
                                                                     aaCCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGcc                                                                               <  2:2657821/150‑1 (MQ=255)
                                                                     aaCCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGcc                                                                               <  2:4178540/150‑1 (MQ=255)
                                                                        ccACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTcc                                                                            >  1:1915805/1‑150 (MQ=255)
                                                                        ccACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTcc                                                                            >  1:1099030/1‑150 (MQ=255)
                                                                         cACTCCTTCGGCACAATATTTTTGCTATCTACCCTCATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                                                                           <  1:695454/150‑1 (MQ=255)
                                                                         cACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                                                                           <  2:54270/150‑1 (MQ=255)
                                                                           cTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAtt                                                                         <  2:2936607/150‑1 (MQ=255)
                                                                           cTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAtt                                                                         <  2:1433800/150‑1 (MQ=255)
                                                                              cTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAt                                                                      <  1:3876863/150‑1 (MQ=255)
                                                                                 cGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACt                                                                   <  2:651194/150‑1 (MQ=255)
                                                                                  ggCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTg                                                                  >  1:4159067/1‑150 (MQ=255)
                                                                                   gCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGt                                                                 >  2:2336403/1‑150 (MQ=255)
                                                                                    cacaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGtt                                                                <  1:3985720/150‑1 (MQ=255)
                                                                                    cacaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGtt                                                                <  2:1308347/150‑1 (MQ=255)
                                                                                    cacaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGtt                                                                <  2:1540802/150‑1 (MQ=255)
                                                                                     acaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGttt                                                               >  2:892366/1‑150 (MQ=255)
                                                                                     acaATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGttt                                                               >  1:3081845/1‑150 (MQ=255)
                                                                                      caATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGtttt                                                              <  1:2034918/150‑1 (MQ=255)
                                                                                        atatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTta                                                            >  1:3739938/1‑150 (MQ=255)
                                                                                        atatTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTta                                                            >  2:1326667/1‑150 (MQ=255)
                                                                                              ttGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACa                                                      <  2:2278480/150‑1 (MQ=255)
                                                                                                     cTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGt                                               >  2:1115482/1‑150 (MQ=255)
                                                                                                      tACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGtt                                              >  2:1615225/1‑150 (MQ=255)
                                                                                                      tACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGtt                                              >  2:3088846/1‑150 (MQ=255)
                                                                                                            catcatCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCaaaa                                        >  1:1286127/1‑150 (MQ=255)
                                                                                                             atcatcAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAt                                       >  1:1580778/1‑150 (MQ=255)
                                                                                                             atcatcAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAt                                       >  1:662576/1‑150 (MQ=255)
                                                                                                              tcatcaACTGCTCAAAGCCTGTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAtt                                      <  2:3963591/150‑1 (MQ=255)
                                                                                                                 tcaACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTaaa                                   >  1:2614887/1‑150 (MQ=255)
                                                                                                                   aaCTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAAc                                 >  2:1818756/1‑150 (MQ=255)
                                                                                                                      tGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACcac                              >  2:3615735/1‑150 (MQ=255)
                                                                                                                         tCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAg                           <  2:2704472/150‑1 (MQ=255)
                                                                                                                              gCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATg                      >  2:3265184/1‑150 (MQ=255)
                                                                                                                              gCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATg                      <  2:2043646/150‑1 (MQ=255)
                                                                                                                               ccTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGa                     >  2:3242959/1‑150 (MQ=255)
                                                                                                                                ctttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAg                    >  2:3978628/1‑150 (MQ=255)
                                                                                                                                ctttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAg                    <  1:1494661/150‑1 (MQ=255)
                                                                                                                                 tttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgt                   >  1:4420939/1‑150 (MQ=255)
                                                                                                                                 tttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgt                   >  1:737729/1‑150 (MQ=255)
                                                                                                                                  ttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCCTGACGCCTCCATTGATACTGTTTTTCTATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAggg                  >  2:680883/1‑148 (MQ=255)
                                                                                                                                  ttctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgtg                  >  1:396763/1‑150 (MQ=255)
                                                                                                                                   tctttACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAgtgt                 >  1:3286878/1‑150 (MQ=255)
                                                                                                                                      ttACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGa              <  1:1696221/150‑1 (MQ=255)
                                                                                                                                       tACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAt             >  1:1697317/1‑150 (MQ=255)
                                                                                                                                       tACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAt             >  2:2588819/1‑150 (MQ=255)
                                                                                                                                        aCCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAtt            >  2:3742401/1‑150 (MQ=255)
                                                                                                                                        aCCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAtt            <  1:2320218/150‑1 (MQ=255)
                                                                                                                                         ccGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTa           <  2:1674302/150‑1 (MQ=255)
                                                                                                                                         ccGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTa           <  1:3729099/150‑1 (MQ=255)
                                                                                                                                         ccGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTa           >  2:4117827/1‑150 (MQ=255)
                                                                                                                                         ccGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTa           <  2:1552210/150‑1 (MQ=255)
                                                                                                                                           gTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATg         <  1:760075/150‑1 (MQ=255)
                                                                                                                                           gTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATg         <  2:2197624/150‑1 (MQ=255)
                                                                                                                                           gTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATg         >  2:771909/1‑150 (MQ=255)
                                                                                                                                              tGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGa      >  1:4303620/1‑150 (MQ=255)
                                                                                                                                              tGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGa      >  1:4303556/1‑150 (MQ=255)
                                                                                                                                               gTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAg     <  1:2898495/150‑1 (MQ=255)
                                                                                                                                                ttCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGc    <  1:2659135/150‑1 (MQ=255)
                                                                                                                                                 tCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCTTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCa   >  2:2078862/1‑150 (MQ=255)
                                                                                                                                                  cccTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAg  <  1:2976252/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG  >  CP009273/2074531‑2074826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGAC  >  CP009273/2074531‑2074828
                                                                                                                                                     |                                                                                                                                                    
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCT                                                                                                                                                      >  SRR19973080.54809/1‑150 (MQ=60)
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCT                                                                                                                                                      <  SRR19973080.426047/150‑1 (MQ=60)
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCT                                                                                                                                                      <  SRR19973080.426267/150‑1 (MQ=60)
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCT                                                                                                                                                      <  SRR19973080.1863861/150‑1 (MQ=60)
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCA                                                                                                                                                      <  SRR19973080.789734/150‑1 (MQ=60)
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCA                                                                                                                                                      <  SRR19973080.616160/150‑1 (MQ=60)
 ACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTC                                                                                                                                                     >  SRR19973080.1624852/1‑150 (MQ=60)
      CACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCC                                                                                                                                                >  SRR19973080.643987/1‑150 (MQ=60)
      CACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCC                                                                                                                                                >  SRR19973080.4003941/1‑150 (MQ=60)
      CACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCC                                                                                                                                                >  SRR19973080.643310/1‑150 (MQ=60)
        CGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCC‑TCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAAC                                                                                                                                             <  SRR19973080.4555411/150‑1 (MQ=60)
         GACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAAC                                                                                                                                             >  SRR19973080.4526330/1‑150 (MQ=60)
          ACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACG                                                                                                                                            >  SRR19973080.555839/1‑150 (MQ=60)
          ACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACG                                                                                                                                            >  SRR19973080.555833/1‑150 (MQ=60)
              TGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGAT                                                                                                                                        >  SRR19973080.3705825/1‑150 (MQ=60)
              TGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGAT                                                                                                                                        <  SRR19973080.1926612/150‑1 (MQ=60)
                  GCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAA                                                                                                                                    <  SRR19973080.530524/150‑1 (MQ=60)
                    AGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAC                                                                                                                                  >  SRR19973080.2922594/1‑150 (MQ=60)
                    AGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAC                                                                                                                                  >  SRR19973080.2922546/1‑150 (MQ=60)
                    AGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAC                                                                                                                                  <  SRR19973080.1258193/150‑1 (MQ=60)
                    AGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAC                                                                                                                                  <  SRR19973080.2695851/150‑1 (MQ=60)
                    AGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAAC                                                                                                                                  >  SRR19973080.4476986/1‑150 (MQ=60)
                       GTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTG                                                                                                                               <  SRR19973080.422031/150‑1 (MQ=60)
                         AATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCA                                                                                                                             <  SRR19973080.2555797/150‑1 (MQ=60)
                         AATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCA                                                                                                                             <  SRR19973080.1258510/150‑1 (MQ=60)
                            TTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACG                                                                                                                          <  SRR19973080.2285154/150‑1 (MQ=60)
                            TTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGGTCCCTCGGGCCAACGAGATGGAAACCTGCAACG                                                                                                                          <  SRR19973080.2285439/150‑1 (MQ=60)
                                CGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTAC                                                                                                                      >  SRR19973080.3434303/1‑150 (MQ=60)
                                      GTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCT                                                                                                                <  SRR19973080.1290057/150‑1 (MQ=60)
                                      GTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCT                                                                                                                <  SRR19973080.1290043/150‑1 (MQ=60)
                                         TTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTT                                                                                                             <  SRR19973080.1385805/150‑1 (MQ=60)
                                          TTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTC                                                                                                            >  SRR19973080.837047/1‑150 (MQ=60)
                                           TCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCC                                                                                                           <  SRR19973080.4282712/150‑1 (MQ=60)
                                           TCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCC                                                                                                           <  SRR19973080.4282839/150‑1 (MQ=60)
                                           TCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCC                                                                                                           <  SRR19973080.4282724/150‑1 (MQ=60)
                                           TCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCC                                                                                                           <  SRR19973080.3169991/150‑1 (MQ=60)
                                            CATCTGGATTGTCGTAATAGACAGCAACCCACCCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCT                                                                                                          <  SRR19973080.1902317/150‑1 (MQ=60)
                                             ATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTG                                                                                                         >  SRR19973080.1871298/1‑150 (MQ=60)
                                             ATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTG                                                                                                         >  SRR19973080.2495647/1‑150 (MQ=60)
                                                 GGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTA                                                                                                     <  SRR19973080.3606295/150‑1 (MQ=60)
                                                  GATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAA                                                                                                    >  SRR19973080.3657635/1‑150 (MQ=60)
                                                  GATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAA                                                                                                    <  SRR19973080.2542500/150‑1 (MQ=60)
                                                  GATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAA                                                                                                    <  SRR19973080.2538940/150‑1 (MQ=60)
                                                     TGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCT                                                                                                 <  SRR19973080.1754190/150‑1 (MQ=60)
                                                     TGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCT                                                                                                 <  SRR19973080.1755274/150‑1 (MQ=60)
                                                          TAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAG                                                                                            <  SRR19973080.1804474/150‑1 (MQ=60)
                                                          TAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAG                                                                                            <  SRR19973080.1804545/150‑1 (MQ=60)
                                                             TAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTC                                                                                         >  SRR19973080.3612766/1‑150 (MQ=60)
                                                                        CCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCC                                                                              >  SRR19973080.4214080/1‑150 (MQ=60)
                                                                        CCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCC                                                                              >  SRR19973080.1105359/1‑150 (MQ=60)
                                                                        CCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCC                                                                              >  SRR19973080.1926593/1‑150 (MQ=60)
                                                                         CACTCCTTCGGCACAATATTTTTGCTATCTACCCTCATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCA                                                                             <  SRR19973080.699536/150‑1 (MQ=60)
                                                                         CACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCA                                                                             >  SRR19973080.2907169/1‑150 (MQ=60)
                                                                         CACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCA                                                                             >  SRR19973080.2905782/1‑150 (MQ=60)
                                                                         CACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCA                                                                             <  SRR19973080.698578/150‑1 (MQ=60)
                                                                          ACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAT                                                                            >  SRR19973080.17473/1‑150 (MQ=60)
                                                                             CCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGA                                                                         <  SRR19973080.4014313/150‑1 (MQ=60)
                                                                              CTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAT                                                                        <  SRR19973080.3898218/150‑1 (MQ=60)
                                                                              CTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGAT                                                                        <  SRR19973080.618418/150‑1 (MQ=60)
                                                                                  GGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTG                                                                    >  SRR19973080.4181509/1‑150 (MQ=60)
                                                                                    CACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTT                                                                  <  SRR19973080.4007647/150‑1 (MQ=60)
                                                                                     ACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTT                                                                 >  SRR19973080.3098683/1‑150 (MQ=60)
                                                                                      CAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTT                                                                <  SRR19973080.2046482/150‑1 (MQ=60)
                                                                                      CAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTT                                                                <  SRR19973080.2046518/150‑1 (MQ=60)
                                                                                      CAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATAATGTTTT                                                                >  SRR19973080.4520775/1‑150 (MQ=60)
                                                                                        ATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTA                                                              >  SRR19973080.3759812/1‑150 (MQ=60)
                                                                                        ATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTA                                                              >  SRR19973080.3760379/1‑150 (MQ=60)
                                                                                           TTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATAT                                                           <  SRR19973080.1357523/150‑1 (MQ=60)
                                                                                            TTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATA                                                          <  SRR19973080.2634975/150‑1 (MQ=60)
                                                                                             TTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATAC                                                         <  SRR19973080.4091526/150‑1 (MQ=60)
                                                                                                 CTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTA                                                     <  SRR19973080.3881380/150‑1 (MQ=60)
                                                                                                 CTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTA                                                     <  SRR19973080.1505273/150‑1 (MQ=60)
                                                                                                  TATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTAT                                                    >  SRR19973080.742668/1‑150 (MQ=60)
                                                                                                     CTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGT                                                 >  SRR19973080.1974379/1‑150 (MQ=60)
                                                                                                      TACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTT                                                >  SRR19973080.3428571/1‑150 (MQ=60)
                                                                                                         CCACCTCATCTTCTGCTCAAAGCCTTTCTTGACCGTCGGTTCCCTCGGGACAACGAGATGGAAACCTGCTACGGTACGTTTCTCTTCCTGCTTAATCTAGTAGTTCTTGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCA                                             <  SRR19973080.1364439/150‑1 (MQ=60)
                                                                                                            CATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAA                                          >  SRR19973080.1293901/1‑150 (MQ=60)
                                                                                                             ATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAT                                         >  SRR19973080.666543/1‑150 (MQ=60)
                                                                                                             ATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAT                                         >  SRR19973080.1590002/1‑150 (MQ=60)
                                                                                                             ATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAT                                         >  SRR19973080.1590007/1‑150 (MQ=60)
                                                                                                             ATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAAT                                         >  SRR19973080.1590082/1‑150 (MQ=60)
                                                                                                              TCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATT                                        >  SRR19973080.4551554/1‑150 (MQ=60)
                                                                                                              TCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATT                                        >  SRR19973080.1407747/1‑150 (MQ=60)
                                                                                                                ATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAA                                      >  SRR19973080.4563688/1‑150 (MQ=60)
                                                                                                                 TCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAA                                     >  SRR19973080.2629073/1‑150 (MQ=60)
                                                                                                                       GCTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACA                               <  SRR19973080.4443527/150‑1 (MQ=60)
                                                                                                                        CTCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAA                              <  SRR19973080.3962448/150‑1 (MQ=60)
                                                                                                                         TCAAAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAG                             >  SRR19973080.3097228/1‑150 (MQ=60)
                                                                                                                            AAGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGTTGGAAACCTGCAACGTTACGTTTCTCTTCCTGATTAATCTCGTAGTTCATGACGCCTCCATTGATACTTTTTTTATATACAGTATAGTTGAAAAATTAAAACCACAAGGAA                          <  SRR19973080.3155327/150‑1 (MQ=60)
                                                                                                                             AGCCTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAAT                         <  SRR19973080.708444/150‑1 (MQ=60)
                                                                                                                                CTTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAG                      <  SRR19973080.1503174/150‑1 (MQ=60)
                                                                                                                                 TTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGT                     >  SRR19973080.741937/1‑150 (MQ=60)
                                                                                                                                 TTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGT                     >  SRR19973080.741425/1‑150 (MQ=60)
                                                                                                                                 TTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGT                     >  SRR19973080.3014473/1‑150 (MQ=60)
                                                                                                                                 TTTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGT                     >  SRR19973080.4444977/1‑150 (MQ=60)
                                                                                                                                  TTCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTG                    >  SRR19973080.399117/1‑150 (MQ=60)
                                                                                                                                   TCTTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAAACACAAGGAATGAGTGT                   >  SRR19973080.3304913/1‑150 (MQ=60)
                                                                                                                                     TTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTG                 <  SRR19973080.911428/150‑1 (MQ=60)
                                                                                                                                     TTTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTG                 <  SRR19973080.3245161/150‑1 (MQ=60)
                                                                                                                                      TTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGA                >  SRR19973080.952725/1‑150 (MQ=60)
                                                                                                                                      TTACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGA                >  SRR19973080.1913310/1‑150 (MQ=60)
                                                                                                                                      TTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGA                <  SRR19973080.1706096/150‑1 (MQ=60)
                                                                                                                                      TTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGA                <  SRR19973080.1706112/150‑1 (MQ=60)
                                                                                                                                       TACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAT               >  SRR19973080.1283452/1‑150 (MQ=60)
                                                                                                                                       TACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGAT               >  SRR19973080.1707194/1‑150 (MQ=60)
                                                                                                                                        ACCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATT              <  SRR19973080.2333094/150‑1 (MQ=60)
                                                                                                                                         CCGTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTA             <  SRR19973080.3749510/150‑1 (MQ=60)
                                                                                                                                           GTCTGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATG           <  SRR19973080.764345/150‑1 (MQ=60)
                                                                                                                                              TGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGA        >  SRR19973080.4327137/1‑150 (MQ=60)
                                                                                                                                              TGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGA        >  SRR19973080.4327073/1‑150 (MQ=60)
                                                                                                                                              TGTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGA        >  SRR19973080.4327080/1‑150 (MQ=60)
                                                                                                                                               GTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAG       <  SRR19973080.2914324/150‑1 (MQ=60)
                                                                                                                                               GTTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAG       <  SRR19973080.2914345/150‑1 (MQ=60)
                                                                                                                                                TTCCCTCGGGCCAACGAGATGTAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGC      <  SRR19973080.3875532/150‑1 (MQ=60)
                                                                                                                                                TTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGC      <  SRR19973080.2673543/150‑1 (MQ=60)
                                                                                                                                                TTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGC      <  SRR19973080.2673575/150‑1 (MQ=60)
                                                                                                                                                TTCCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGC      >  SRR19973080.4296148/1‑150 (MQ=60)
                                                                                                                                                  CCCTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAG    <  SRR19973080.2992329/150‑1 (MQ=60)
                                                                                                                                                    CTCGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGAC  <  SRR19973080.4336113/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGTATAGTTGCAAAATTAAAACCACAAGGAATGAGTGTTGATTATGCGAGCAGAC  >  CP009273/2074531‑2074828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑