Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,098,995 C→A 100% G182* (GGA→TGA)  wbbI ← d‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,098,9950CA98.9% 657.6 / ‑1.2 175G182* (GGA→TGA) wbbId‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (72/101);  total (72/103)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 176 total aligned reads displayed.

GTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACA  >  CP009273/2098850‑2099141
                                                                                                                                                 |                                                                                                                                                  
   tCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGta                                                                                                                                             <  2:3668329/150‑1 (MQ=255)
    cGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtat                                                                                                                                            <  2:1576413/150‑1 (MQ=255)
    cGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtat                                                                                                                                            <  2:1173858/150‑1 (MQ=255)
      aCAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtatat                                                                                                                                          <  2:885043/150‑1 (MQ=255)
         gAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATg                                                                                                                                       <  1:4009897/150‑1 (MQ=255)
              tcCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGa                                                                                                                                  <  1:1230444/150‑1 (MQ=255)
               ccATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGaa                                                                                                                                 >  1:3519076/1‑150 (MQ=255)
                    ccAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACAttt                                                                                                                            <  1:2511611/150‑1 (MQ=255)
                     cAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTa                                                                                                                           >  2:2966661/1‑150 (MQ=255)
                       aaTGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATg                                                                                                                         >  2:3182008/1‑150 (MQ=255)
                          gAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCt                                                                                                                      <  2:2468242/150‑1 (MQ=255)
                           aGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTa                                                                                                                     <  2:1969714/150‑1 (MQ=255)
                            gTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAg                                                                                                                    <  1:3305304/150‑1 (MQ=255)
                            gTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAg                                                                                                                    <  1:3940069/150‑1 (MQ=255)
                                aaaTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAg                                                                                                                <  1:1898386/150‑1 (MQ=255)
                                aaaTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAg                                                                                                                <  1:4283019/150‑1 (MQ=255)
                                 aaTTGCATGCCTGTGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAgg                                                                                                               <  2:451358/150‑1 (MQ=255)
                                 aaTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAgg                                                                                                               >  2:2230622/1‑150 (MQ=255)
                                    tGCATGCCTGGGATGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTg                                                                                                            <  1:1704639/150‑1 (MQ=255)
                                    tGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTg                                                                                                            <  1:2352770/150‑1 (MQ=255)
                                           cTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCat                                                                                                     >  2:1146241/1‑150 (MQ=255)
                                            tGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCata                                                                                                    <  2:4048624/150‑1 (MQ=255)
                                            tGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCata                                                                                                    >  1:2313306/1‑150 (MQ=255)
                                             gggAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCatat                                                                                                   <  2:1875192/150‑1 (MQ=255)
                                                aGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATg                                                                                                <  1:2846105/150‑1 (MQ=255)
                                                aGGTTAATCTGTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATg                                                                                                <  1:2846136/150‑1 (MQ=255)
                                                  gTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAc                                                                                              >  1:3397747/1‑150 (MQ=255)
                                                   ttAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAcc                                                                                             >  2:4119990/1‑150 (MQ=255)
                                                   ttAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAcc                                                                                             <  1:403383/150‑1 (MQ=255)
                                                     aaTCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAcccc                                                                                           >  2:732589/1‑150 (MQ=255)
                                                     aaTCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAcccc                                                                                           >  2:2352409/1‑150 (MQ=255)
                                                     aaTCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAcccc                                                                                           <  2:4522437/150‑1 (MQ=255)
                                                             ccGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCt                                                                                   <  1:4039984/150‑1 (MQ=255)
                                                                 agaTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATc                                                                               >  2:1675288/1‑150 (MQ=255)
                                                                       aGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACGCCTCGTTGCTTATCCGTAAc                                                                         >  1:2135001/1‑150 (MQ=255)
                                                                       aGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAAc                                                                         <  1:3203054/150‑1 (MQ=255)
                                                                       aGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATAATTCAGTATATATGAAAGAACATTTATGACTCGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAAc                                                                         >  1:2134470/1‑150 (MQ=255)
                                                                        gCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACa                                                                        <  1:1301986/150‑1 (MQ=255)
                                                                        gCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACa                                                                        <  2:1694984/150‑1 (MQ=255)
                                                                        gCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACa                                                                        <  2:1695439/150‑1 (MQ=255)
                                                                         cATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAt                                                                       <  2:2944935/150‑1 (MQ=255)
                                                                           tCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtct                                                                     <  2:3213165/150‑1 (MQ=255)
                                                                            cAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtctc                                                                    <  2:1471672/150‑1 (MQ=255)
                                                                            cAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtctc                                                                    <  2:1640487/150‑1 (MQ=255)
                                                                              aaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGa                                                                  >  1:3465220/1‑150 (MQ=255)
                                                                               aaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGAt                                                                 <  1:78684/150‑1 (MQ=255)
                                                                               aaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGAt                                                                 <  1:2112865/150‑1 (MQ=255)
                                                                                    ccAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTcc                                                            >  2:2394264/1‑150 (MQ=255)
                                                                                      aaGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCcac                                                          >  1:4567117/1‑150 (MQ=255)
                                                                                        gATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACAt                                                        >  2:526430/1‑150 (MQ=255)
                                                                                          tatTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCa                                                      <  1:201092/150‑1 (MQ=255)
                                                                                           atTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAg                                                     >  1:431576/1‑150 (MQ=255)
                                                                                               aGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATga                                                 <  1:2966661/150‑1 (MQ=255)
                                                                                               aGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATga                                                 <  1:2966611/150‑1 (MQ=255)
                                                                                                  aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGAc                                              <  2:81413/150‑1 (MQ=255)
                                                                                                   ttATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACg                                             <  1:2051916/150‑1 (MQ=255)
                                                                                                     aTCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAg                                           >  2:1898406/1‑150 (MQ=255)
                                                                                                     aTCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAg                                           >  2:1898535/1‑150 (MQ=255)
                                                                                                     aTCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAg                                           >  2:2058863/1‑150 (MQ=255)
                                                                                                      tCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAgg                                          <  1:1173094/150‑1 (MQ=255)
                                                                                                      tCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAgg                                          <  1:3690647/150‑1 (MQ=255)
                                                                                                      tCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAgg                                          <  1:3691968/150‑1 (MQ=255)
                                                                                                        tttATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTa                                        <  2:1749715/150‑1 (MQ=255)
                                                                                                        tttATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTa                                        <  2:4339846/150‑1 (MQ=255)
                                                                                                        tttATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTa                                        <  2:3324234/150‑1 (MQ=255)
                                                                                                          tATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAt                                      <  1:1526036/150‑1 (MQ=255)
                                                                                                          tATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAt                                      <  1:1525771/150‑1 (MQ=255)
                                                                                                          tATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAt                                      <  2:2749623/150‑1 (MQ=255)
                                                                                                           aTTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                     <  1:3982648/150‑1 (MQ=255)
                                                                                                           aTTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                     <  1:3335854/150‑1 (MQ=255)
                                                                                                           aTTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                     >  2:1545803/1‑150 (MQ=255)
                                                                                                           aTTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                     >  2:683301/1‑150 (MQ=255)
                                                                                                              ttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCaaa                                  >  1:2614115/1‑150 (MQ=255)
                                                                                                                cATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                                >  1:506464/1‑150 (MQ=255)
                                                                                                                cATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                                >  1:507061/1‑150 (MQ=255)
                                                                                                                cATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                                <  2:3875566/150‑1 (MQ=255)
                                                                                                                cATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAata                                >  2:1326284/1‑150 (MQ=255)
                                                                                                                 aTAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAatat                               <  2:3043221/150‑1 (MQ=255)
                                                                                                                    gTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATAtttt                            >  2:3992544/1‑150 (MQ=255)
                                                                                                                      tGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTa                          >  2:3342190/1‑150 (MQ=255)
                                                                                                                            cAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCtt                    <  2:261539/150‑1 (MQ=255)
                                                                                                                            cAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCtt                    <  2:4447682/150‑1 (MQ=255)
                                                                                                                               agagagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGa                 <  2:1389139/150‑1 (MQ=255)
                                                                                                                                 agagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtt               <  1:3081481/150‑1 (MQ=255)
                                                                                                                                 agagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtt               <  2:741871/150‑1 (MQ=255)
                                                                                                                                   agTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtttt             <  2:3719439/150‑1 (MQ=255)
                                                                                                                                     tAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTAt           <  1:1146241/150‑1 (MQ=255)
                                                                                                                                       aaaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATcc         >  2:2285473/1‑150 (MQ=255)
                                                                                                                                        aaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCt        <  2:4249160/150‑1 (MQ=255)
                                                                                                                                         aTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTg       >  2:3947336/1‑150 (MQ=255)
                                                                                                                                         aTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTg       >  2:3946033/1‑150 (MQ=255)
                                                                                                                                         aTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTg       <  1:4477060/150‑1 (MQ=255)
                                                                                                                                         aTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTg       <  2:1806173/150‑1 (MQ=255)
                                                                                                                                          tctcatcatTAAGTATATATTAAATAACATTTATTCATAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAAAATCTCGATTCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTga      <  2:2285001/147‑1 (MQ=255)
                                                                                                                                          tCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATTTCTTTGATTTTATCCTga      >  1:810275/1‑150 (MQ=255)
                                                                                                                                          tCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTga      <  2:3692623/150‑1 (MQ=255)
                                                                                                                                          tCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTga      <  2:4665252/150‑1 (MQ=255)
                                                                                                                                           cGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTgag     <  1:2563613/150‑1 (MQ=255)
                                                                                                                                              aTCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAt                >  1:3992515/1‑136 (MQ=255)
                                                                                                                                              aTCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACa  >  2:2213948/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACA  >  CP009273/2098850‑2099141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACAT  >  CP009273/2098846‑2099142
                                                                                                                                                     |                                                                                                                                                   
ACAGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTTAGCATCAAAACTTACAAGATATTTAGGATTATATTTATTTTCATAGTTGAAACCAAAGAGAGTAAAATCGCATCA                                                                                                                                                     >  SRR19973082.2299268/1‑150 (MQ=60)
ACAGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCA                                                                                                                                                     >  SRR19973082.4693447/1‑150 (MQ=60)
ACAGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCA                                                                                                                                                     >  SRR19973082.3715461/1‑150 (MQ=60)
 CAGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCAT                                                                                                                                                    >  SRR19973082.4566903/1‑150 (MQ=60)
    GTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCA                                                                                                                                                 >  SRR19973082.2766286/1‑150 (MQ=60)
            AGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATAT                                                                                                                                         <  SRR19973082.4048144/150‑1 (MQ=60)
             GAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATG                                                                                                                                        <  SRR19973082.4034403/150‑1 (MQ=60)
                  TCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGA                                                                                                                                   <  SRR19973082.1238850/150‑1 (MQ=60)
                   CCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAA                                                                                                                                  >  SRR19973082.3540537/1‑150 (MQ=60)
                   CCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAA                                                                                                                                  >  SRR19973082.3540350/1‑150 (MQ=60)
                        CCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTT                                                                                                                             <  SRR19973082.2527288/150‑1 (MQ=60)
                                GTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAG                                                                                                                     <  SRR19973082.3325726/150‑1 (MQ=60)
                                GTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAG                                                                                                                     <  SRR19973082.3325308/150‑1 (MQ=60)
                                GTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAG                                                                                                                     <  SRR19973082.3964031/150‑1 (MQ=60)
                                GTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAG                                                                                                                     <  SRR19973082.3918483/150‑1 (MQ=60)
                                  CCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAA                                                                                                                   <  SRR19973082.3984668/150‑1 (MQ=60)
                                    AAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAG                                                                                                                 <  SRR19973082.4308979/150‑1 (MQ=60)
                                    AAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAG                                                                                                                 <  SRR19973082.1910553/150‑1 (MQ=60)
                                    AAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAG                                                                                                                 <  SRR19973082.4746570/150‑1 (MQ=60)
                                        TGCATGCCTGGGATGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTG                                                                                                             <  SRR19973082.1715969/150‑1 (MQ=60)
                                        TGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTG                                                                                                             <  SRR19973082.793488/150‑1 (MQ=60)
                                        TGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTG                                                                                                             <  SRR19973082.2367263/150‑1 (MQ=60)
                                         GCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCACATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGC                                                                                                            <  SRR19973082.1300561/150‑1 (MQ=60)
                                                TGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATA                                                                                                     >  SRR19973082.4592926/1‑150 (MQ=60)
                                                TGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATA                                                                                                     >  SRR19973082.2327674/1‑150 (MQ=60)
                                                TGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATA                                                                                                     >  SRR19973082.791751/1‑150 (MQ=60)
                                                    AGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATG                                                                                                 <  SRR19973082.2863585/150‑1 (MQ=60)
                                                    AGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATG                                                                                                 <  SRR19973082.2863591/150‑1 (MQ=60)
                                                    AGGTTAATCTGTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATG                                                                                                 <  SRR19973082.2863616/150‑1 (MQ=60)
                                                      GTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGAC                                                                                               >  SRR19973082.3418741/1‑150 (MQ=60)
                                                       TTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACC                                                                                              <  SRR19973082.405977/150‑1 (MQ=60)
                                                       TTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACC                                                                                              <  SRR19973082.405924/150‑1 (MQ=60)
                                                          ATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCT                                                                                           <  SRR19973082.3174060/150‑1 (MQ=60)
                                                              TTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTT                                                                                       >  SRR19973082.2126757/1‑150 (MQ=60)
                                                                 CCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCT                                                                                    <  SRR19973082.4064747/150‑1 (MQ=60)
                                                                           AGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACGCCTCGTTGCTTATCCGTAAC                                                                          >  SRR19973082.2148575/1‑150 (MQ=60)
                                                                           AGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAAC                                                                          <  SRR19973082.3222558/150‑1 (MQ=60)
                                                                           AGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATAATTCAGTATATATGAAAGAACATTTATGACTCGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAAC                                                                          >  SRR19973082.2148043/1‑150 (MQ=60)
                                                                           AGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTAATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCACTCGTTGCTTATCCGTAAC                                                                          >  SRR19973082.2148561/1‑150 (MQ=60)
                                                                            GCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACA                                                                         <  SRR19973082.1310982/150‑1 (MQ=60)
                                                                               TCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCT                                                                      <  SRR19973082.3567058/150‑1 (MQ=60)
                                                                               TCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCT                                                                      <  SRR19973082.345258/150‑1 (MQ=60)
                                                                                  AAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGA                                                                   >  SRR19973082.3486503/1‑150 (MQ=60)
                                                                                   AACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGAT                                                                  <  SRR19973082.79526/150‑1 (MQ=60)
                                                                                   AACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGAT                                                                  <  SRR19973082.2126314/150‑1 (MQ=60)
                                                                                   AACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGAT                                                                  <  SRR19973082.1914238/150‑1 (MQ=60)
                                                                                          AAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCAC                                                           >  SRR19973082.4594855/1‑150 (MQ=60)
                                                                                              TATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCA                                                       <  SRR19973082.202761/150‑1 (MQ=60)
                                                                                               ATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAG                                                      >  SRR19973082.434506/1‑150 (MQ=60)
                                                                                                 TTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGAT                                                    <  SRR19973082.928924/150‑1 (MQ=60)
                                                                                                 TTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGAT                                                    <  SRR19973082.2344801/150‑1 (MQ=60)
                                                                                                   AGGTTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGA                                                  <  SRR19973082.2984932/150‑1 (MQ=60)
                                                                                                   AGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGA                                                  <  SRR19973082.2984982/150‑1 (MQ=60)
                                                                                                    GGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAG                                                 >  SRR19973082.3090314/1‑150 (MQ=60)
                                                                                                    GGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAG                                                 <  SRR19973082.2106608/150‑1 (MQ=60)
                                                                                                     GATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGA                                                <  SRR19973082.429781/150‑1 (MQ=60)
                                                                                                       TTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACG                                              <  SRR19973082.2064968/150‑1 (MQ=60)
                                                                                                          TCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGG                                           <  SRR19973082.1180761/150‑1 (MQ=60)
                                                                                                          TCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGG                                           <  SRR19973082.3713471/150‑1 (MQ=60)
                                                                                                          TCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGG                                           <  SRR19973082.3714802/150‑1 (MQ=60)
                                                                                                          TCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGG                                           <  SRR19973082.4481622/150‑1 (MQ=60)
                                                                                                            TTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTA                                         >  SRR19973082.1213049/1‑150 (MQ=60)
                                                                                                              TATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAT                                       <  SRR19973082.1535872/150‑1 (MQ=60)
                                                                                                              TATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAT                                       <  SRR19973082.1536139/150‑1 (MQ=60)
                                                                                                               ATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATC                                      <  SRR19973082.3329564/150‑1 (MQ=60)
                                                                                                               ATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATC                                      >  SRR19973082.1885424/1‑150 (MQ=60)
                                                                                                               ATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATC                                      <  SRR19973082.3356488/150‑1 (MQ=60)
                                                                                                               ATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATC                                      <  SRR19973082.4006939/150‑1 (MQ=60)
                                                                                                               ATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATC                                      <  SRR19973082.4006891/150‑1 (MQ=60)
                                                                                                                TTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCA                                     >  SRR19973082.292219/1‑150 (MQ=60)
                                                                                                                  TTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAA                                   >  SRR19973082.242993/1‑150 (MQ=60)
                                                                                                                  TTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAA                                   >  SRR19973082.2630321/1‑150 (MQ=60)
                                                                                                                    CATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATA                                 >  SRR19973082.510674/1‑150 (MQ=60)
                                                                                                                    CATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATA                                 >  SRR19973082.510069/1‑150 (MQ=60)
                                                                                                                                 AAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTT                    <  SRR19973082.1529345/150‑1 (MQ=60)
                                                                                                                                     AGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATT                <  SRR19973082.3100311/150‑1 (MQ=60)
                                                                                                                                         TAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTAT            <  SRR19973082.1153827/150‑1 (MQ=60)
                                                                                                                                         TAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTAT            >  SRR19973082.1595174/1‑150 (MQ=60)
                                                                                                                                             ATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTG        <  SRR19973082.4504266/150‑1 (MQ=60)
                                                                                                                                              TCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATTTCTTTGATTTTATCCTGA       >  SRR19973082.815495/1‑150 (MQ=60)
                                                                                                                                               CGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAG      <  SRR19973082.2579597/150‑1 (MQ=60)
                                                                                                                                               CGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAG      >  SRR19973082.2555987/1‑150 (MQ=60)
                                                                                                                                                  ATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATagatcggaagagca   >  SRR19973082.4016850/1‑136 (MQ=60)
                                                                                                                                                   TCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACAT  >  SRR19973082.1955991/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
ACAGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACAT  >  CP009273/2098846‑2099142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑