Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,098,995 C→A 100% G182* (GGA→TGA)  wbbI ← d‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,098,9950CA99.5% 653.1 / ‑4.2 197G182* (GGA→TGA) wbbId‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (120/76);  total (120/77)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 197 total aligned reads displayed.

AGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACA  >  CP009273/2098848‑2099141
                                                                                                                                                   |                                                                                                                                                  
aGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCAtt                                                                                                                                                  >  2:1925974/1‑150 (MQ=255)
 ggTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTc                                                                                                                                                 <  2:3941483/150‑1 (MQ=255)
  gTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCa                                                                                                                                                <  2:4178902/150‑1 (MQ=255)
   tttCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAg                                                                                                                                               <  1:1064390/150‑1 (MQ=255)
     tCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGta                                                                                                                                             >  2:1335086/1‑150 (MQ=255)
      cGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtat                                                                                                                                            <  1:3305442/150‑1 (MQ=255)
       gACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtata                                                                                                                                           >  1:1570009/1‑150 (MQ=255)
        aCAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtatat                                                                                                                                          <  2:3854034/150‑1 (MQ=255)
         cAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGtatata                                                                                                                                         <  1:2986816/150‑1 (MQ=255)
          aGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTatatat                                                                                                                                        >  1:1910508/1‑150 (MQ=255)
                tcCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGa                                                                                                                                  <  1:2482782/150‑1 (MQ=255)
                tcCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGa                                                                                                                                  >  2:1692546/1‑150 (MQ=255)
                 ccATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGaa                                                                                                                                 >  1:963132/1‑150 (MQ=255)
                    tCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACAt                                                                                                                              >  2:1027158/1‑150 (MQ=255)
                     cccAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACAtt                                                                                                                             >  1:1607363/1‑150 (MQ=255)
                      ccAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACAttt                                                                                                                            >  1:2556386/1‑150 (MQ=255)
                      ccAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACAttt                                                                                                                            >  2:837335/1‑150 (MQ=255)
                        aaaTGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTAt                                                                                                                          <  1:2406623/150‑1 (MQ=255)
                          aTGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGc                                                                                                                        >  1:364454/1‑150 (MQ=255)
                           tGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGcc                                                                                                                       <  1:4879194/150‑1 (MQ=255)
                            gAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCt                                                                                                                      >  1:3921651/1‑150 (MQ=255)
                            gAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCt                                                                                                                      <  2:1188191/150‑1 (MQ=255)
                            gAGTCAAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCt                                                                                                                      <  2:551055/150‑1 (MQ=255)
                             aGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTa                                                                                                                     >  1:2171237/1‑150 (MQ=255)
                             aGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTa                                                                                                                     >  1:2171070/1‑150 (MQ=255)
                              gTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAg                                                                                                                    <  2:5052252/150‑1 (MQ=255)
                                ccAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGaa                                                                                                                  <  1:3844433/150‑1 (MQ=255)
                                ccAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGaa                                                                                                                  >  2:2025411/1‑150 (MQ=255)
                                ccAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGaa                                                                                                                  <  2:1995656/150‑1 (MQ=255)
                                 cAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGaaa                                                                                                                 >  2:4598359/1‑150 (MQ=255)
                                 cAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGaaa                                                                                                                 <  1:3917054/150‑1 (MQ=255)
                                    aTTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGt                                                                                                              >  2:2900904/1‑150 (MQ=255)
                                     ttGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGGGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGtt                                                                                                             >  2:667760/1‑150 (MQ=255)
                                     ttGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGtt                                                                                                             <  2:92079/150‑1 (MQ=255)
                                       gCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGc                                                                                                           >  2:4025758/1‑150 (MQ=255)
                                            ccTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCa                                                                                                      >  2:477569/1‑150 (MQ=255)
                                               gggAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCatat                                                                                                   >  2:2559434/1‑150 (MQ=255)
                                                        aTCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCt                                                                                          <  2:2588469/150‑1 (MQ=255)
                                                        aTCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCt                                                                                          <  2:2588511/150‑1 (MQ=255)
                                                          cTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATAGTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCg                                                                                        >  2:2965566/1‑150 (MQ=255)
                                                            tttCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGtt                                                                                      <  2:3096911/150‑1 (MQ=255)
                                                            tttCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGtt                                                                                      <  2:3096997/150‑1 (MQ=255)
                                                                 ggAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTa                                                                                 >  2:2987030/1‑150 (MQ=255)
                                                                  gagaTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTAt                                                                                <  1:4663576/150‑1 (MQ=255)
                                                                   cgaTTGAGCATCAAAACTTCCAAGATATTGAGGATTATCTTTATTTTCATAGTTGACCCCAAAGAGAGTACAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATAGGACCCCTCGTTGCTTATc                                                                               <  1:1352839/149‑1 (MQ=255)
                                                                   agaTTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATc                                                                               >  2:1383264/1‑150 (MQ=255)
                                                                       tGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTa                                                                           >  1:284383/1‑150 (MQ=255)
                                                                       tGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTa                                                                           >  1:597225/1‑150 (MQ=255)
                                                                        gAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTaa                                                                          >  1:5142639/1‑150 (MQ=255)
                                                                         aGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAAc                                                                         >  2:1153205/1‑150 (MQ=255)
                                                                         aGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAAc                                                                         <  2:3349960/150‑1 (MQ=255)
                                                                           cATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAt                                                                       >  1:4102147/1‑150 (MQ=255)
                                                                            aTCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtc                                                                      >  2:4879015/1‑150 (MQ=255)
                                                                             tCAAAACTTACAAGATATTTAGGATTATCTTTATTTTCTTAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCTTATATGACCCCTCTTTTCTTATCCGTAACAtct                                                                     <  2:4505520/150‑1 (MQ=255)
                                                                              cAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAtctc                                                                    >  1:4635489/1‑150 (MQ=255)
                                                                               aaaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTCGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCg                                                                   >  2:4325447/1‑150 (MQ=255)
                                                                               aaaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCg                                                                   >  2:2054723/1‑150 (MQ=255)
                                                                               aaaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCg                                                                   >  2:2499428/1‑150 (MQ=255)
                                                                                aaaCTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGa                                                                  <  1:2950566/150‑1 (MQ=255)
                                                                                   cTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGc                                                               >  1:4558665/1‑150 (MQ=255)
                                                                                             atTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAg                                                     >  2:4918464/1‑150 (MQ=255)
                                                                                              tttAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGa                                                    >  1:3306666/1‑150 (MQ=255)
                                                                                               ttAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGAt                                                   >  2:3741871/1‑150 (MQ=255)
                                                                                                 aGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATga                                                 >  1:922804/1‑150 (MQ=255)
                                                                                                  ggATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgag                                                >  1:4173723/1‑150 (MQ=255)
                                                                                                  ggATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATgag                                                >  1:708536/1‑150 (MQ=255)
                                                                                                    cttATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGACTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGAc                                              >  2:398597/2‑150 (MQ=255)
                                                                                                    aTTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGAc                                              <  2:2136466/150‑1 (MQ=255)
                                                                                                     ttATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACg                                             >  1:3197952/1‑150 (MQ=255)
                                                                                                       aTCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGT‑ACATCTCGATGCTCCACATCAGATGAGACGAgg                                          <  2:1234476/150‑1 (MQ=255)
                                                                                                        tCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAgg                                          <  1:3573287/150‑1 (MQ=255)
                                                                                                        tCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAgg                                          <  1:806364/150‑1 (MQ=255)
                                                                                                            tATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAt                                      >  1:4042325/1‑150 (MQ=255)
                                                                                                             aTTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATc                                     <  1:2816158/150‑1 (MQ=255)
                                                                                                               tttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCaa                                   >  2:4549585/1‑150 (MQ=255)
                                                                                                               tttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCaa                                   <  1:3173605/150‑1 (MQ=255)
                                                                                                               tttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCaa                                   >  1:105436/1‑150 (MQ=255)
                                                                                                               tttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCaa                                   >  2:656201/1‑150 (MQ=255)
                                                                                                               tttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCaa                                   >  1:4867245/1‑150 (MQ=255)
                                                                                                                ttCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCaaa                                  >  1:4084337/1‑150 (MQ=255)
                                                                                                                 tCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAat                                 >  2:3284706/1‑150 (MQ=255)
                                                                                                                     aGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATAttt                             >  1:2328182/1‑150 (MQ=255)
                                                                                                                      gTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATAtttt                            >  2:1448812/1‑150 (MQ=255)
                                                                                                                        tGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTa                          >  2:1487364/1‑150 (MQ=255)
                                                                                                                              cAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCtt                    <  2:3189806/150‑1 (MQ=255)
                                                                                                                              cAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCtt                    >  2:2947744/1‑150 (MQ=255)
                                                                                                                              cAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCtt                    >  2:2272508/1‑150 (MQ=255)
                                                                                                                               aaaGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCttt                   >  2:3666181/1‑150 (MQ=255)
                                                                                                                                  gagagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAt                >  1:889919/1‑150 (MQ=255)
                                                                                                                                   agagTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtt               >  2:786335/1‑150 (MQ=255)
                                                                                                                                     agTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtttt             >  2:2440868/1‑150 (MQ=255)
                                                                                                                                     agTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAtttt             <  1:4978775/150‑1 (MQ=255)
                                                                                                                                      gTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTa            <  2:4558665/150‑1 (MQ=255)
                                                                                                                                       tAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTAt           >  1:3543467/1‑150 (MQ=255)
                                                                                                                                       tAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTAt           >  1:622235/1‑150 (MQ=255)
                                                                                                                                        aaaaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATc          >  2:102839/1‑150 (MQ=255)
                                                                                                                                         aaaTCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATcc         >  2:672393/1‑150 (MQ=255)
                                                                                                                                              gCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTgaga    >  2:1076434/1‑150 (MQ=255)
                                                                                                                                                aTCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACa  <  2:2241645/150‑1 (MQ=255)
                                                                                                                                                aTCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACa  >  2:2511158/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGTTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACA  >  CP009273/2098848‑2099141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACATAT  >  CP009273/2098851‑2099144
                                                                                                                                                |                                                                                                                                                     
TTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAG                                                                                                                                                  <  SRR19973063.1067622/150‑1 (MQ=60)
  TCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTA                                                                                                                                                <  SRR19973063.3574742/150‑1 (MQ=60)
   CGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTAT                                                                                                                                               <  SRR19973063.3316016/150‑1 (MQ=60)
    GACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATA                                                                                                                                              >  SRR19973063.1574830/1‑150 (MQ=60)
      CAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATA                                                                                                                                            <  SRR19973063.2996350/150‑1 (MQ=60)
       AGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATAT                                                                                                                                           <  SRR19973063.839908/150‑1 (MQ=60)
       AGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATAT                                                                                                                                           >  SRR19973063.1916485/1‑150 (MQ=60)
             TCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGA                                                                                                                                     <  SRR19973063.2490712/150‑1 (MQ=60)
              CCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAA                                                                                                                                    >  SRR19973063.966033/1‑150 (MQ=60)
                ATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACA                                                                                                                                  >  SRR19973063.4578586/1‑150 (MQ=60)
                  CCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATT                                                                                                                                >  SRR19973063.3300379/1‑150 (MQ=60)
                  CCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATT                                                                                                                                >  SRR19973063.1612284/1‑150 (MQ=60)
                   CCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTT                                                                                                                               >  SRR19973063.2564531/1‑150 (MQ=60)
                     AAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTAT                                                                                                                             <  SRR19973063.2414323/150‑1 (MQ=60)
                       ATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGC                                                                                                                           >  SRR19973063.365499/1‑150 (MQ=60)
                       ATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGC                                                                                                                           >  SRR19973063.365410/1‑150 (MQ=60)
                        TGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCC                                                                                                                          <  SRR19973063.4894929/150‑1 (MQ=60)
                         GAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCT                                                                                                                         >  SRR19973063.3934161/1‑150 (MQ=60)
                          AGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTA                                                                                                                        >  SRR19973063.2178033/1‑150 (MQ=60)
                          AGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTA                                                                                                                        >  SRR19973063.2177969/1‑150 (MQ=60)
                          AGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTA                                                                                                                        >  SRR19973063.2177865/1‑150 (MQ=60)
                             CCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAA                                                                                                                     <  SRR19973063.3856656/150‑1 (MQ=60)
                             CCAAAGTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGTA                                                                                                                     <  SRR19973063.4900256/150‑1 (MQ=60)
                              CAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAA                                                                                                                    <  SRR19973063.3929531/150‑1 (MQ=60)
                              CAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAA                                                                                                                    <  SRR19973063.3929553/150‑1 (MQ=60)
                                AATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGG                                                                                                                  >  SRR19973063.4705221/1‑150 (MQ=60)
                                                            CCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCT                                                                                      <  SRR19973063.145401/150‑1 (MQ=60)
                                                               GAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTAT                                                                                   <  SRR19973063.4678451/150‑1 (MQ=60)
                                                               GAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTAT                                                                                   <  SRR19973063.4678221/150‑1 (MQ=60)
                                                                CGATTGAGCATCAAAACTTCCAAGATATTGAGGATTATCTTTATTTTCATAGTTGACCCCAAAGAGAGTACAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATAGGACCCCTCGTTGCTTATC                                                                                  <  SRR19973063.1356901/150‑1 (MQ=60)
                                                                AGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATC                                                                                  >  SRR19973063.896208/1‑150 (MQ=60)
                                                                    TGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTA                                                                              >  SRR19973063.599001/1‑150 (MQ=60)
                                                                    TGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTA                                                                              >  SRR19973063.598986/1‑150 (MQ=60)
                                                                    TGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTA                                                                              >  SRR19973063.285223/1‑150 (MQ=60)
                                                                     GAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAA                                                                             >  SRR19973063.5159190/1‑150 (MQ=60)
                                                                        CATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAT                                                                          >  SRR19973063.4115267/1‑150 (MQ=60)
                                                                        CATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACAT                                                                          >  SRR19973063.4115291/1‑150 (MQ=60)
                                                                         ATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATC                                                                         >  SRR19973063.4052567/1‑150 (MQ=60)
                                                                           CAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTC                                                                       <  SRR19973063.3451991/150‑1 (MQ=60)
                                                                           CAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTC                                                                       >  SRR19973063.4650301/1‑150 (MQ=60)
                                                                            AAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCG                                                                      >  SRR19973063.522883/1‑150 (MQ=60)
                                                                             AAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGA                                                                     <  SRR19973063.2959996/150‑1 (MQ=60)
                                                                             AAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGA                                                                     <  SRR19973063.2958171/150‑1 (MQ=60)
                                                                                CTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCATCGTTGCTTATCCGTAACATCTCGATGC                                                                  >  SRR19973063.4573264/1‑150 (MQ=60)
                                                                                       GATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACAT                                                           >  SRR19973063.1247491/1‑150 (MQ=60)
                                                                                        ATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATC                                                          <  SRR19973063.4074921/150‑1 (MQ=60)
                                                                                           TTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGA                                                       >  SRR19973063.3317229/1‑150 (MQ=60)
                                                                                           TTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGA                                                       >  SRR19973063.3317241/1‑150 (MQ=60)
                                                                                           TTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGA                                                       >  SRR19973063.3189878/1‑150 (MQ=60)
                                                                                            TTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGAT                                                      <  SRR19973063.499825/150‑1 (MQ=60)
                                                                                             TAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGACAGCATATATGACCCCTCGTTGCTTATACGTAACATCTCGATGCTCAACATCAGATG                                                     >  SRR19973063.684028/1‑150 (MQ=60)
                                                                                              AGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGA                                                    >  SRR19973063.925947/1‑150 (MQ=60)
                                                                                              AGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGA                                                    >  SRR19973063.925538/1‑150 (MQ=60)
                                                                                              AGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGA                                                    >  SRR19973063.41688/1‑150 (MQ=60)
                                                                                               GGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAG                                                   >  SRR19973063.4187059/1‑150 (MQ=60)
                                                                                               GGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAG                                                   >  SRR19973063.710619/1‑150 (MQ=60)
                                                                                                  TTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACG                                                >  SRR19973063.3208233/1‑150 (MQ=60)
                                                                                                     TCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGG                                             <  SRR19973063.808689/150‑1 (MQ=60)
                                                                                                     TCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGG                                             <  SRR19973063.3584746/150‑1 (MQ=60)
                                                                                                         TATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAAT                                         >  SRR19973063.4055279/1‑150 (MQ=60)
                                                                                                          ATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATC                                        <  SRR19973063.2825304/150‑1 (MQ=60)
                                                                                                          ATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATC                                        <  SRR19973063.2825211/150‑1 (MQ=60)
                                                                                                           TTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCA                                       <  SRR19973063.2481588/150‑1 (MQ=60)
                                                                                                            TTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAA                                      >  SRR19973063.4882932/1‑150 (MQ=60)
                                                                                                            TTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAA                                      >  SRR19973063.105794/1‑150 (MQ=60)
                                                                                                            TTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAA                                      <  SRR19973063.3183819/150‑1 (MQ=60)
                                                                                                             TTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAA                                     <  SRR19973063.3931720/150‑1 (MQ=60)
                                                                                                             TTCATAGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAA                                     >  SRR19973063.4097417/1‑150 (MQ=60)
                                                                                                                  AGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTT                                >  SRR19973063.2335384/1‑150 (MQ=60)
                                                                                                                  AGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTT                                >  SRR19973063.2335634/1‑150 (MQ=60)
                                                                                                                  AGTTGACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTT                                >  SRR19973063.2335813/1‑150 (MQ=60)
                                                                                                                       ACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATG                           >  SRR19973063.4427672/1‑150 (MQ=60)
                                                                                                                       ACACCAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATG                           >  SRR19973063.2563816/1‑150 (MQ=60)
                                                                                                                           CAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTT                       <  SRR19973063.1634034/150‑1 (MQ=60)
                                                                                                                           CAAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTT                       >  SRR19973063.1597771/1‑150 (MQ=60)
                                                                                                                            AAAGAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTT                      <  SRR19973063.3061059/150‑1 (MQ=60)
                                                                                                                               GAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAT                   >  SRR19973063.892496/1‑150 (MQ=60)
                                                                                                                               GAGAGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGAT                   <  SRR19973063.3671405/150‑1 (MQ=60)
                                                                                                                                  AGTAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTT                <  SRR19973063.4994870/150‑1 (MQ=60)
                                                                                                                                    TAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTAT              >  SRR19973063.624063/1‑150 (MQ=60)
                                                                                                                                    TAAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTAT              >  SRR19973063.3554820/1‑150 (MQ=60)
                                                                                                                                     AAAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATC             >  SRR19973063.2981344/1‑150 (MQ=60)
                                                                                                                                      AAATCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCC            <  SRR19973063.786700/150‑1 (MQ=60)
                                                                                                                                         TCGCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGA         >  SRR19973063.1991439/1‑150 (MQ=60)
                                                                                                                                           GCATCATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGA       >  SRR19973063.2663867/1‑150 (MQ=60)
                                                                                                                                                ATTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACATAT  <  SRR19973063.2033535/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
TTTCGACAGAATCTCCATCCCAAATGAGTCCAAATTGCATGCCTGGGAGGTTAATCTTTTCCGGAGATTGAGCATCAAAACTTCCAAGATATTTAGGATTATCTTTATTTTCATAGTTGACACCAAAGAGAGTAAAATCGCATCCTTCAGTATATATGAAAGAACATTTATGCCTAGAAAGGTTGCCAGCATATATGACCCCTCGTTGCTTATCCGTAACATCTCGATGCTCCACATCAGATGAGACGAGGTAATCAAATATTTTTATGTCTTTGATTTTATCCTGAGACATAT  >  CP009273/2098851‑2099144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑