Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,144,474 C→A 100% intergenic (‑8/‑192) yegI ← / → yegJ protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,144,4740CA96.6% 412.6 / 5.1 117intergenic (‑8/‑192)yegI/yegJprotein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (41/71);  total (43/74)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

GGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTC  >  CP009273/2144327‑2144619
                                                                                                                                                   |                                                                                                                                                 
ggcggcGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTagc                                                                                                                                                 <  2:209590/150‑1 (MQ=255)
ggcggcGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTagc                                                                                                                                                 >  1:2265866/1‑150 (MQ=255)
       tgtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGt                                                                                                                                          <  2:3467744/150‑1 (MQ=255)
        gtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGtt                                                                                                                                         <  2:940901/150‑1 (MQ=255)
           aTAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttata                                                                                                                                      <  2:4663947/150‑1 (MQ=255)
              aaTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatatta                                                                                                                                   <  1:4161810/150‑1 (MQ=255)
               aTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatattat                                                                                                                                  <  2:1797870/150‑1 (MQ=255)
                tCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCCCCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatattata                                                                                                                                 <  2:24483/150‑1 (MQ=255)
                tCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatattata                                                                                                                                 <  2:24479/150‑1 (MQ=255)
                tCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatattata                                                                                                                                 <  2:24489/150‑1 (MQ=255)
                       gACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAAt                                                                                                                          >  1:2815319/1‑150 (MQ=255)
                        aCGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATg                                                                                                                         >  2:2095138/1‑150 (MQ=255)
                        aCGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATg                                                                                                                         <  2:3196908/150‑1 (MQ=255)
                        aCGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATg                                                                                                                         <  1:2812276/150‑1 (MQ=255)
                          gCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGaa                                                                                                                       <  1:2166864/150‑1 (MQ=255)
                           cTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaat                                                                                                                      <  1:1203296/150‑1 (MQ=255)
                            tATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaata                                                                                                                     >  1:130501/1‑150 (MQ=255)
                            tATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaata                                                                                                                     <  1:251060/150‑1 (MQ=255)
                            tATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaata                                                                                                                     <  1:251841/150‑1 (MQ=255)
                             aTCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaataa                                                                                                                    <  2:4437058/150‑1 (MQ=255)
                                gACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACCTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAAtaataa                                                                                                                 <  2:139955/150‑1 (MQ=255)
                                 aCAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATaac                                                                                                                >  1:272805/1‑150 (MQ=255)
                                   aaaCTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATaacaa                                                                                                              >  2:3970358/1‑150 (MQ=255)
                                    aaCTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATaacaac                                                                                                             <  1:3927937/150‑1 (MQ=255)
                                            gATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAtt                                                                                                     <  2:1895931/150‑1 (MQ=255)
                                            gATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAtt                                                                                                     <  2:1895930/150‑1 (MQ=255)
                                            gATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAtt                                                                                                     <  2:4279565/150‑1 (MQ=255)
                                                  aTAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatt                                                                                               >  1:2809393/1‑150 (MQ=255)
                                                  aTAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatt                                                                                               <  2:4208744/150‑1 (MQ=255)
                                                    aaaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattct                                                                                             >  2:3112936/1‑148 (MQ=255)
                                                    aaaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattat                                                                                             <  2:4176245/150‑1 (MQ=255)
                                                    aaaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattat                                                                                             >  2:2382996/1‑150 (MQ=255)
                                                    aaaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattat                                                                                             >  1:3125039/1‑150 (MQ=255)
                                                    aaaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattat                                                                                             >  1:381405/1‑150 (MQ=255)
                                                      aCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAttt                                                                                           <  2:2543037/150‑1 (MQ=255)
                                                      aCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAttt                                                                                           <  1:1263568/150‑1 (MQ=255)
                                                      aCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAttt                                                                                           <  1:2447470/150‑1 (MQ=255)
                                                            ccTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTatat                                                                                     <  1:4207358/150‑1 (MQ=255)
                                                            ccTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTatat                                                                                     <  2:2840744/150‑1 (MQ=255)
                                                             cTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATg                                                                                    <  2:3374963/150‑1 (MQ=255)
                                                             cTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATg                                                                                    <  2:3374831/150‑1 (MQ=255)
                                                             cTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATg                                                                                    <  2:3374713/150‑1 (MQ=255)
                                                               tCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGTGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATggg                                                                                  <  2:3417234/150‑1 (MQ=255)
                                                                caccacCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATgggg                                                                                 >  1:172667/1‑150 (MQ=255)
                                                                 accaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGTGGa                                                                                >  1:2567227/1‑150 (MQ=255)
                                                                 accaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGa                                                                                <  2:3062590/150‑1 (MQ=255)
                                                                 accaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGa                                                                                <  2:3061740/150‑1 (MQ=255)
                                                                    accTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGaaaa                                                                             <  1:1572329/150‑1 (MQ=255)
                                                                     ccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAAc                                                                            <  2:1173792/150‑1 (MQ=255)
                                                                         tGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCAc                                                                        <  2:2394868/150‑1 (MQ=255)
                                                                           cccATTTCACGGCCAAGATTTGTCATTTCACATGTCTATTTAAATAATTTTATATTTGTTTTCATAATTTTTTGCGTGTAGTTATATTTTATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCctcta                                                                      <  2:61301/150‑2 (MQ=255)
                                                                                ttCACGGCCACGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGaa                                                                 <  1:2666379/150‑1 (MQ=255)
                                                                                ttCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGaa                                                                 >  1:2980156/1‑150 (MQ=255)
                                                                                 tCACGGCCAAGTGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACATCTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGTAt                                                                <  2:274225/150‑1 (MQ=255)
                                                                                 tCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAAt                                                                <  2:4603176/150‑1 (MQ=255)
                                                                                 tCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAAt                                                                <  2:2222711/150‑1 (MQ=255)
                                                                                  cACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATa                                                               <  1:1445841/150‑1 (MQ=255)
                                                                                    cGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaaa                                                             <  2:1274135/150‑1 (MQ=255)
                                                                                     ggCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaaaa                                                            <  2:4689837/150‑1 (MQ=255)
                                                                                        cAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAGAAATg                                                         <  1:4752617/150‑1 (MQ=255)
                                                                                        cAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATg                                                         >  2:3152731/1‑150 (MQ=255)
                                                                                        cAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATg                                                         <  1:1335373/150‑1 (MQ=255)
                                                                                        cAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATg                                                         <  1:2374630/150‑1 (MQ=255)
                                                                                        cAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATg                                                         <  1:1335413/150‑1 (MQ=255)
                                                                                         aaGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGa                                                        >  2:1584755/1‑150 (MQ=255)
                                                                                          agagTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAATAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGAt                                                       >  1:1264071/1‑150 (MQ=255)
                                                                                            agTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATaa                                                     <  1:3016682/150‑1 (MQ=255)
                                                                                                     ttCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAtttt                                            <  2:3780923/150‑1 (MQ=255)
                                                                                                      tCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTa                                           <  1:3402993/150‑1 (MQ=255)
                                                                                                      tCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTa                                           <  1:3402138/150‑1 (MQ=255)
                                                                                                      tCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTa                                           <  2:2258262/150‑1 (MQ=255)
                                                                                                        aCCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATc                                         <  1:2514429/150‑1 (MQ=255)
                                                                                                           tGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAgg                                      <  1:4509217/150‑1 (MQ=255)
                                                                                                           tGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAgg                                      >  2:3399181/1‑150 (MQ=255)
                                                                                                                 tGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaa                                >  1:2100126/1‑150 (MQ=255)
                                                                                                                    aaaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcacttc                             >  2:3524838/1‑150 (MQ=255)
                                                                                                                    aaaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattc                             >  2:3524612/1‑150 (MQ=255)
                                                                                                                     aaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattca                            >  1:1623624/1‑150 (MQ=255)
                                                                                                                     aaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattca                            >  1:440237/1‑150 (MQ=255)
                                                                                                                     aaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattca                            >  1:1623751/1‑150 (MQ=255)
                                                                                                                      aTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattcaa                           >  2:3611271/1‑150 (MQ=255)
                                                                                                                         cTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAAttt                        <  2:1472476/150‑1 (MQ=255)
                                                                                                                            ttATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTATATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCgt                     >  2:4746743/1‑149 (MQ=255)
                                                                                                                             tataTTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAACTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGc                    >  1:4602580/1‑150 (MQ=255)
                                                                                                                               tatTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCt                  >  2:2203156/1‑150 (MQ=255)
                                                                                                                               tatTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCt                  >  2:2203210/1‑150 (MQ=255)
                                                                                                                                   tGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATa              >  2:3017991/1‑150 (MQ=255)
                                                                                                                                   tGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATa              >  2:84219/1‑150 (MQ=255)
                                                                                                                                   tGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATa              >  2:84012/1‑150 (MQ=255)
                                                                                                                                    gTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATaa             >  1:684579/1‑150 (MQ=255)
                                                                                                                                    gTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATaa             >  2:1603995/1‑150 (MQ=255)
                                                                                                                                     ttttCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAAt            >  1:4193454/1‑150 (MQ=255)
                                                                                                                                         cATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGAtt        <  1:3919063/150‑1 (MQ=255)
                                                                                                                                           taataTTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACACTTCAATTTCGGCCTGATAATGATTaa      >  2:3171255/1‑150 (MQ=255)
                                                                                                                                           taataTTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATaaa      >  2:2648748/1‑150 (MQ=255)
                                                                                                                                            aataTTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAAc     <  2:315423/150‑1 (MQ=255)
                                                                                                                                            aataTTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAAc     >  1:3096421/1‑150 (MQ=255)
                                                                                                                                             atatTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACt    >  1:464662/1‑150 (MQ=255)
                                                                                                                                              tatTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACtt   <  1:318681/150‑1 (MQ=255)
                                                                                                                                               atTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTc  <  2:923396/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTC  >  CP009273/2144327‑2144619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCACA  >  CP009273/2144325‑2144623
                                                                                                                                                     |                                                                                                                                                     
GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTA                                                                                                                                                       <  SRR19973082.1258727/150‑1 (MQ=60)
GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTA                                                                                                                                                       <  SRR19973082.3666009/150‑1 (MQ=60)
  GGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGC                                                                                                                                                     >  SRR19973082.2280068/1‑150 (MQ=60)
                AATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTA                                                                                                                                       <  SRR19973082.4187280/150‑1 (MQ=60)
                  TCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATA                                                                                                                                     <  SRR19973082.1401977/150‑1 (MQ=60)
                         GACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAAT                                                                                                                              >  SRR19973082.2832541/1‑150 (MQ=60)
                          ACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATG                                                                                                                             <  SRR19973082.2829456/150‑1 (MQ=60)
                            GCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAA                                                                                                                           <  SRR19973082.2180608/150‑1 (MQ=60)
                             CTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAAT                                                                                                                          <  SRR19973082.1211388/150‑1 (MQ=60)
                              TATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATA                                                                                                                         >  SRR19973082.131689/1‑150 (MQ=60)
                              TATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATA                                                                                                                         <  SRR19973082.253775/150‑1 (MQ=60)
                              TATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATA                                                                                                                         <  SRR19973082.252990/150‑1 (MQ=60)
                                   ACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAAC                                                                                                                    >  SRR19973082.274830/1‑150 (MQ=60)
                                      AACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAAC                                                                                                                 <  SRR19973082.3951868/150‑1 (MQ=60)
                                           CTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACG                                                                                                            <  SRR19973082.1852035/150‑1 (MQ=60)
                                                TATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAA                                                                                                       <  SRR19973082.3087607/150‑1 (MQ=60)
                                                    ATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATT                                                                                                   >  SRR19973082.2826531/1‑150 (MQ=60)
                                                    ATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAAACATTAAAATT                                                                                                   >  SRR19973082.2825907/1‑150 (MQ=60)
                                                      AAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAT                                                                                                 <  SRR19973082.525836/150‑1 (MQ=60)
                                                      AAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAT                                                                                                 >  SRR19973082.383833/1‑150 (MQ=60)
                                                      AAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAT                                                                                                 >  SRR19973082.383819/1‑150 (MQ=60)
                                                      AAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAT                                                                                                 >  SRR19973082.3144001/1‑150 (MQ=60)
                                                        ACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTT                                                                                               <  SRR19973082.1272270/150‑1 (MQ=60)
                                                        ACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTT                                                                                               <  SRR19973082.2462709/150‑1 (MQ=60)
                                                        ACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTT                                                                                               <  SRR19973082.2462837/150‑1 (MQ=60)
                                                              CCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATAT                                                                                         <  SRR19973082.4230915/150‑1 (MQ=60)
                                                              CCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATAT                                                                                         <  SRR19973082.4233032/150‑1 (MQ=60)
                                                                  CACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGG                                                                                     >  SRR19973082.174144/1‑150 (MQ=60)
                                                                   ACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGTGGA                                                                                    >  SRR19973082.2583224/1‑150 (MQ=60)
                                                                      ACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAA                                                                                 <  SRR19973082.1582567/150‑1 (MQ=60)
                                                                                  TTCACGGCCACGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAA                                                                     <  SRR19973082.2682790/150‑1 (MQ=60)
                                                                                  TTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAA                                                                     >  SRR19973082.2998558/1‑150 (MQ=60)
                                                                                    CACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATA                                                                   <  SRR19973082.1455646/150‑1 (MQ=60)
                                                                                          CAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAGAAATG                                                             <  SRR19973082.4781102/150‑1 (MQ=60)
                                                                                          CAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATG                                                             <  SRR19973082.1344621/150‑1 (MQ=60)
                                                                                          CAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATG                                                             <  SRR19973082.1344581/150‑1 (MQ=60)
                                                                                          CAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATG                                                             <  SRR19973082.2389203/150‑1 (MQ=60)
                                                                                            AGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAATAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGAT                                                           >  SRR19973082.1272776/1‑150 (MQ=60)
                                                                                              AGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAA                                                         <  SRR19973082.3035267/150‑1 (MQ=60)
                                                                                                        TCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTA                                               <  SRR19973082.3423154/150‑1 (MQ=60)
                                                                                                        TCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTA                                               <  SRR19973082.3424016/150‑1 (MQ=60)
                                                                                                          ACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATC                                             <  SRR19973082.2530120/150‑1 (MQ=60)
                                                                                                             TGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGG                                          <  SRR19973082.4536641/150‑1 (MQ=60)
                                                                                                                   TGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAA                                    >  SRR19973082.2113485/1‑150 (MQ=60)
                                                                                                                       AATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCA                                >  SRR19973082.1634511/1‑150 (MQ=60)
                                                                                                                       AATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCA                                >  SRR19973082.443257/1‑150 (MQ=60)
                                                                                                                       AATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCA                                >  SRR19973082.1634383/1‑150 (MQ=60)
                                                                                                                               TATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAACTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGC                        >  SRR19973082.4630521/1‑150 (MQ=60)
                                                                                                                                      GTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAA                 >  SRR19973082.689241/1‑150 (MQ=60)
                                                                                                                                       TTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAAT                >  SRR19973082.4219069/1‑150 (MQ=60)
                                                                                                                                           CATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATT            <  SRR19973082.3942966/150‑1 (MQ=60)
                                                                                                                                              AATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAAC         >  SRR19973082.3115309/1‑150 (MQ=60)
                                                                                                                                               ATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACT        >  SRR19973082.467920/1‑150 (MQ=60)
                                                                                                                                                TATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTT       <  SRR19973082.320898/150‑1 (MQ=60)
                                                                                                                                                    TAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCAC   >  SRR19973082.1745193/1‑150 (MQ=60)
                                                                                                                                                    TAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCAC   >  SRR19973082.1745190/1‑150 (MQ=60)
                                                                                                                                                     AGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCACA  <  SRR19973082.2108472/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCACA  >  CP009273/2144325‑2144623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑