Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,144,474 C→A 69.8% intergenic (‑8/‑192) yegI ← / → yegJ protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,144,4740CA69.8% 162.6 / 119.9 122intergenic (‑8/‑192)yegI/yegJprotein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (18/19);  new base A (26/59);  total (44/78)
Fisher's exact test for biased strand distribution p-value = 6.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACT  >  CP009273/2144325‑2144617
                                                                                                                                                     |                                                                                                                                               
gTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTc                                                                                                                                                 <  1:2344786/150‑1 (MQ=255)
gTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTc                                                                                                                                                 <  1:2344705/150‑1 (MQ=255)
 tGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTcg                                                                                                                                                >  2:1468633/1‑150 (MQ=255)
 tGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTcg                                                                                                                                                >  2:2846779/1‑150 (MQ=255)
  ggcggcGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTcgc                                                                                                                                               <  1:939363/150‑1 (MQ=255)
    cggcgTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCgg                                                                                                                                             <  1:3753741/150‑1 (MQ=255)
    cggcgTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCgg                                                                                                                                             <  1:3756536/150‑1 (MQ=255)
      gcgTGTTATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGt                                                                                                                                           <  1:9422/150‑1 (MQ=255)
       cgTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTa                                                                                                                                          <  1:2007699/150‑1 (MQ=255)
         tgtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGt                                                                                                                                        >  2:4845131/1‑150 (MQ=255)
         tgtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGt                                                                                                                                        >  2:4845215/1‑150 (MQ=255)
         tgtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGt                                                                                                                                        >  1:2500845/1‑150 (MQ=255)
          gtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGtt                                                                                                                                       <  1:886099/150‑1 (MQ=255)
          gtgAGAAATCTGGGCGACGCGATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGGTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGtt                                                                                                                                       <  2:2088952/150‑1 (MQ=255)
             aTAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttata                                                                                                                                    <  1:2251426/150‑1 (MQ=255)
             aTAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttata                                                                                                                                    <  1:1301947/150‑1 (MQ=255)
               aaaTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatatt                                                                                                                                  <  2:4226219/150‑1 (MQ=255)
                aaTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatatta                                                                                                                                 >  2:3042330/1‑150 (MQ=255)
                 aTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGttatattat                                                                                                                                <  1:3445334/150‑1 (MQ=255)
                    ttGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTTTTTTCATAATATTTAGAGGGTAGTTatattatatt                                                                                                                             >  2:4557433/1‑150 (MQ=255)
                      ggCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTc                                                                                                                           <  1:2929812/150‑1 (MQ=255)
                      ggCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTc                                                                                                                           >  1:2132568/1‑150 (MQ=255)
                      ggCGACGCGATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTc                                                                                                                           <  2:4590280/150‑1 (MQ=255)
                           cGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGa                                                                                                                      >  1:2399308/1‑150 (MQ=255)
                             cTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGaat                                                                                                                    <  1:2516221/150‑1 (MQ=255)
                             cTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaat                                                                                                                    <  1:2406039/150‑1 (MQ=255)
                             cTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaat                                                                                                                    >  2:2158824/1‑150 (MQ=255)
                             cTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaat                                                                                                                    >  2:2159559/1‑150 (MQ=255)
                              tATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaata                                                                                                                   <  1:4138553/150‑1 (MQ=255)
                              tATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaata                                                                                                                   <  2:2185732/150‑1 (MQ=255)
                              tATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaata                                                                                                                   <  1:2957648/150‑1 (MQ=255)
                              tATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaata                                                                                                                   <  1:1679078/150‑1 (MQ=255)
                               aTCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTATCGGGTAGTTATATTATATTTAAATGaataa                                                                                                                  >  2:2586428/1‑150 (MQ=255)
                                 cGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAataata                                                                                                                >  1:4580497/1‑150 (MQ=255)
                                 cGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAataata                                                                                                                >  1:1683604/1‑150 (MQ=255)
                                       aCTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACt                                                                                                          <  2:1600547/150‑1 (MQ=255)
                                       aCTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACt                                                                                                          >  2:2252076/1‑150 (MQ=255)
                                           ctcGAGATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAAcc                                                                                                      <  2:3323681/150‑1 (MQ=255)
                                             cGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAt                                                                                                    >  1:2959170/1‑150 (MQ=255)
                                             cGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAt                                                                                                    >  1:4227394/1‑150 (MQ=255)
                                             cGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAt                                                                                                    <  2:757023/150‑1 (MQ=255)
                                              gATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAtt                                                                                                   >  2:2796230/1‑150 (MQ=255)
                                              gATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAtt                                                                                                   <  2:2091583/150‑1 (MQ=255)
                                               atatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTa                                                                                                  <  1:4514707/150‑1 (MQ=255)
                                               atatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTa                                                                                                  <  1:4514739/150‑1 (MQ=255)
                                                tatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTAAATGAATAATAACAACTAACCAttga                                                                                                 >  1:4073190/1‑148 (MQ=255)
                                                tatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaa                                                                                                 <  2:327315/150‑1 (MQ=255)
                                                 atCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaaa                                                                                                >  2:2695266/1‑150 (MQ=255)
                                                  tCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaaaa                                                                                               <  1:3236725/150‑1 (MQ=255)
                                                  tCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaaaa                                                                                               <  1:3237967/150‑1 (MQ=255)
                                                    aTAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatt                                                                                             <  1:3630670/150‑1 (MQ=255)
                                                     tAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatta                                                                                            <  2:276086/150‑1 (MQ=255)
                                                     tAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatta                                                                                            <  2:40002/150‑1 (MQ=255)
                                                      aaaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattat                                                                                           <  1:4686215/150‑1 (MQ=255)
                                                      aaaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattat                                                                                           >  1:3379086/1‑150 (MQ=255)
                                                        aCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAttt                                                                                         <  1:4053209/150‑1 (MQ=255)
                                                             gcCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCtata                                                                                    >  1:1560792/1‑150 (MQ=255)
                                                             gcCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCtata                                                                                    >  1:1560740/1‑150 (MQ=255)
                                                             gcCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCtata                                                                                    >  1:1560678/1‑150 (MQ=255)
                                                             gcCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCtata                                                                                    >  2:1376186/1‑150 (MQ=255)
                                                               cTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATg                                                                                  >  2:1828012/1‑150 (MQ=255)
                                                                ttCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATgg                                                                                 <  1:10558/150‑1 (MQ=255)
                                                                ttCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATgg                                                                                 <  2:26004/150‑1 (MQ=255)
                                                                ttCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATgg                                                                                 <  2:26008/150‑1 (MQ=255)
                                                                 tCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATggg                                                                                <  2:835410/150‑1 (MQ=255)
                                                                   accaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGa                                                                              >  1:4281215/1‑150 (MQ=255)
                                                                    ccaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGaa                                                                             >  2:571614/1‑150 (MQ=255)
                                                                        cTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAAcc                                                                         >  2:4547288/1‑150 (MQ=255)
                                                                               cAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCaa                                                                  >  1:3996735/1‑150 (MQ=255)
                                                                                aGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAg                                                                 >  2:225731/1‑150 (MQ=255)
                                                                                   tCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAAt                                                              <  2:4080611/150‑1 (MQ=255)
                                                                                   tCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAAt                                                              <  2:2729210/150‑1 (MQ=255)
                                                                                   tCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAAt                                                              <  2:2729356/150‑1 (MQ=255)
                                                                                     aCGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaa                                                            <  1:1468633/150‑1 (MQ=255)
                                                                                       ggCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaaaa                                                          <  2:3966007/150‑1 (MQ=255)
                                                                                       ggCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaaaa                                                          <  2:3966082/150‑1 (MQ=255)
                                                                                         ccAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAAt                                                        <  2:3576251/150‑1 (MQ=255)
                                                                                         ccAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAAt                                                        <  2:3356735/150‑1 (MQ=255)
                                                                                          cAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATg                                                       <  1:1808779/150‑1 (MQ=255)
                                                                                               gTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAAt                                                  <  1:4260814/150‑1 (MQ=255)
                                                                                               gTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAAt                                                  <  1:1807315/150‑1 (MQ=255)
                                                                                                 ggTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGc                                                <  2:4488889/150‑1 (MQ=255)
                                                                                                   tCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGTGGAAAACCCACTCAAGAATAAAAATGATAATGCaa                                              >  2:2600922/1‑150 (MQ=255)
                                                                                                     aaTTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAtt                                            <  2:4815012/150‑1 (MQ=255)
                                                                                                       ttCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAtttt                                          <  1:604870/150‑1 (MQ=255)
                                                                                                         cACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAt                                        >  1:713956/1‑150 (MQ=255)
                                                                                                         cACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAt                                        <  1:4229951/150‑1 (MQ=255)
                                                                                                               tCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGAt                                  <  2:1132636/150‑1 (MQ=255)
                                                                                                               tCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGAt                                  <  1:964385/150‑1 (MQ=255)
                                                                                                                    gTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaat                             >  2:2662539/1‑150 (MQ=255)
                                                                                                                         tACTTTTATATTTGTTTTCATAATATTTAGCGGGTATTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattcaat                        <  1:3563326/150‑1 (MQ=255)
                                                                                                                          aCTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattcaatt                       >  1:2785225/1‑150 (MQ=255)
                                                                                                                          aCTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattcaatt                       >  1:2785167/1‑150 (MQ=255)
                                                                                                                           attttATATTTGTTTTCATAATATTTCGCGTGTATTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTATATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAAttt                      <  1:4557433/149‑1 (MQ=255)
                                                                                                                              ttATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCgg                   <  1:1065599/150‑1 (MQ=255)
                                                                                                                              ttATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCgg                   <  1:1065514/150‑1 (MQ=255)
                                                                                                                               tataTTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGc                  >  1:501436/1‑150 (MQ=255)
                                                                                                                                         ttCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCAACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCTGCCTGATAATGa        >  2:4571055/1‑150 (MQ=255)
                                                                                                                                           cATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGAtt      <  2:3525452/150‑1 (MQ=255)
                                                                                                                                               atatTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACt  >  1:3219406/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACT  >  CP009273/2144325‑2144617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCACA  >  CP009273/2144325‑2144623
                                                                                                                                                     |                                                                                                                                                     
GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTC                                                                                                                                                       <  SRR19973081.2359317/150‑1 (MQ=60)
GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTC                                                                                                                                                       <  SRR19973081.2359398/150‑1 (MQ=60)
  GGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGC                                                                                                                                                     <  SRR19973081.945703/150‑1 (MQ=60)
    CGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGG                                                                                                                                                   <  SRR19973081.3779566/150‑1 (MQ=60)
    CGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGG                                                                                                                                                   <  SRR19973081.3776750/150‑1 (MQ=60)
      GCGTGTTATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGT                                                                                                                                                 <  SRR19973081.9559/150‑1 (MQ=60)
       CGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTA                                                                                                                                                <  SRR19973081.2020215/150‑1 (MQ=60)
         TGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGT                                                                                                                                              >  SRR19973081.2516435/1‑150 (MQ=60)
          GTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTT                                                                                                                                             <  SRR19973081.892012/150‑1 (MQ=60)
          GTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTT                                                                                                                                             <  SRR19973081.892028/150‑1 (MQ=60)
          GTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTT                                                                                                                                             >  SRR19973081.2499921/1‑150 (MQ=60)
             ATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATA                                                                                                                                          <  SRR19973081.2265659/150‑1 (MQ=60)
             ATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATA                                                                                                                                          <  SRR19973081.1310649/150‑1 (MQ=60)
                 ATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTAT                                                                                                                                      <  SRR19973081.3466529/150‑1 (MQ=60)
                   CTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATAT                                                                                                                                    >  SRR19973081.3436380/1‑150 (MQ=60)
                      GGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTC                                                                                                                                 <  SRR19973081.2947883/150‑1 (MQ=60)
                      GGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTC                                                                                                                                 >  SRR19973081.2146168/1‑150 (MQ=60)
                           CGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGA                                                                                                                            >  SRR19973081.2414088/1‑150 (MQ=60)
                             CTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAAT                                                                                                                          <  SRR19973081.2531900/150‑1 (MQ=60)
                             CTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAAT                                                                                                                          <  SRR19973081.2420845/150‑1 (MQ=60)
                              TATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATA                                                                                                                         <  SRR19973081.2975884/150‑1 (MQ=60)
                              TATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATA                                                                                                                         <  SRR19973081.4163809/150‑1 (MQ=60)
                              TATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATA                                                                                                                         <  SRR19973081.1690064/150‑1 (MQ=60)
                                 CGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATA                                                                                                                      >  SRR19973081.4608293/1‑150 (MQ=60)
                                 CGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATA                                                                                                                      >  SRR19973081.1694628/1‑150 (MQ=60)
                                             CGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAT                                                                                                          >  SRR19973081.4253214/1‑150 (MQ=60)
                                             CGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAT                                                                                                          >  SRR19973081.2977418/1‑150 (MQ=60)
                                               ATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTA                                                                                                        <  SRR19973081.4542009/150‑1 (MQ=60)
                                               ATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTA                                                                                                        <  SRR19973081.4542058/150‑1 (MQ=60)
                                               ATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTA                                                                                                        <  SRR19973081.4542042/150‑1 (MQ=60)
                                               ATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTA                                                                                                        <  SRR19973081.4100212/150‑1 (MQ=60)
                                                TATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTAAATGAATAATAACAACTAACCATTGA                                                                                                       >  SRR19973081.4097849/1‑150 (MQ=60)
                                                  TCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAA                                                                                                     <  SRR19973081.3256217/150‑1 (MQ=60)
                                                  TCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAA                                                                                                     <  SRR19973081.3257464/150‑1 (MQ=60)
                                                    ATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATT                                                                                                   <  SRR19973081.3652599/150‑1 (MQ=60)
                                                      AAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAT                                                                                                 >  SRR19973081.3399859/1‑150 (MQ=60)
                                                      AAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTAT                                                                                                 <  SRR19973081.4714634/150‑1 (MQ=60)
                                                        ACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTT                                                                                               <  SRR19973081.4077676/150‑1 (MQ=60)
                                                        ACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTT                                                                                               <  SRR19973081.1605438/150‑1 (MQ=60)
                                                         CCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTC                                                                                              <  SRR19973081.2266311/150‑1 (MQ=60)
                                                             GCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATA                                                                                          >  SRR19973081.1570855/1‑150 (MQ=60)
                                                             GCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATA                                                                                          >  SRR19973081.1570793/1‑150 (MQ=60)
                                                             GCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATA                                                                                          >  SRR19973081.1570907/1‑150 (MQ=60)
                                                              CCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATAT                                                                                         <  SRR19973081.1444276/150‑1 (MQ=60)
                                                                TTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGG                                                                                       <  SRR19973081.10704/150‑1 (MQ=60)
                                                                   ACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGA                                                                                    >  SRR19973081.4307321/1‑150 (MQ=60)
                                                                               CAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAA                                                                        >  SRR19973081.4021045/1‑150 (MQ=60)
                                                                                     ACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAA                                                                  <  SRR19973081.2864072/150‑1 (MQ=60)
                                                                                     ACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAA                                                                  <  SRR19973081.1478381/150‑1 (MQ=60)
                                                                                          CAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATG                                                             <  SRR19973081.1820574/150‑1 (MQ=60)
                                                                                               GTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAAT                                                        <  SRR19973081.4286816/150‑1 (MQ=60)
                                                                                               GTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAAT                                                        <  SRR19973081.1819102/150‑1 (MQ=60)
                                                                                                    CAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAT                                                   <  SRR19973081.3778016/150‑1 (MQ=60)
                                                                                                       TTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTT                                                <  SRR19973081.609122/150‑1 (MQ=60)
                                                                                                         CACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAT                                              <  SRR19973081.4267094/150‑1 (MQ=60)
                                                                                                         CACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAT                                              >  SRR19973081.718700/1‑150 (MQ=60)
                                                                                                         CACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAT                                              <  SRR19973081.4255784/150‑1 (MQ=60)
                                                                                                               TCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGAT                                        <  SRR19973081.515956/150‑1 (MQ=60)
                                                                                                               TCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGAT                                        <  SRR19973081.970946/150‑1 (MQ=60)
                                                                                                               TCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGAT                                        <  SRR19973081.3126209/150‑1 (MQ=60)
                                                                                                                         TACTTTTATATTTGTTTTCATAATATTTAGCGGGTATTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAAT                              <  SRR19973081.3585060/150‑1 (MQ=60)
                                                                                                                          ACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATT                             >  SRR19973081.2802252/1‑150 (MQ=60)
                                                                                                                          ACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATT                             >  SRR19973081.2802194/1‑150 (MQ=60)
                                                                                                                           ATTTTATATTTGTTTTCATAATATTTCGCGTGTATTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTATATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTT                            <  SRR19973081.4585066/150‑1 (MQ=60)
                                                                                                                            TTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTC                           <  SRR19973081.1872995/150‑1 (MQ=60)
                                                                                                                              TTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGG                         <  SRR19973081.1072710/150‑1 (MQ=60)
                                                                                                                              TTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGG                         <  SRR19973081.1072625/150‑1 (MQ=60)
                                                                                                                               TATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTGAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGC                        >  SRR19973081.505018/1‑150 (MQ=60)
                                                                                                                                               ATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACT        >  SRR19973081.3238835/1‑150 (MQ=60)
                                                                                                                                                    TAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCAC   <  SRR19973081.1134812/150‑1 (MQ=60)
                                                                                                                                                     AGCGGTTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCACA  <  SRR19973081.4677803/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTCCACA  >  CP009273/2144325‑2144623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑