Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,689,722 T→G 13.2% L188* (TTA→TGA)  mltF → membrane‑bound lytic transglycosylase F, murein hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,689,7220TG13.2% 766.4 / 115.0 280L188* (TTA→TGA) mltFmembrane‑bound lytic transglycosylase F, murein hydrolase
Reads supporting (aligned to +/- strand):  ref base T (113/130);  new base G (17/20);  total (130/150)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 284 total aligned reads displayed.

GTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATGGCG  >  CP009273/2689573‑2689871
                                                                                                                                                     |                                                                                                                                                     
 tATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTa                                                                                                                                                      <  2:1650872/150‑1 (MQ=255)
  aTCGCCCACGTAGGCTGGGCAACCTGACGGCGGAGAAACTCACTGTTGCACCGGGTCATGTGTTTTTTAACTATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTATGCTGGATGGTAGACGAAAAAAAAGGCTCTGCGGAATTaa                                                                                                                                                     <  1:2779471/150‑1 (MQ=255)
  aTCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTaa                                                                                                                                                     >  1:224809/1‑150 (MQ=255)
   tCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAAt                                                                                                                                                    <  2:220651/150‑1 (MQ=255)
   tCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAAt                                                                                                                                                    <  2:3841718/150‑1 (MQ=255)
     gCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATgg                                                                                                                                                  <  2:3783199/150‑1 (MQ=255)
          cGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGAt                                                                                                                                             >  1:4284784/1‑150 (MQ=255)
          cGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGAt                                                                                                                                             <  2:4217938/150‑1 (MQ=255)
           gTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATg                                                                                                                                            >  2:413122/1‑150 (MQ=255)
            tACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGt                                                                                                                                           >  1:1381835/1‑150 (MQ=255)
               gCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCAt                                                                                                                                        <  2:1057861/150‑1 (MQ=255)
                cTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATc                                                                                                                                       <  1:222522/150‑1 (MQ=255)
                 tGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCg                                                                                                                                      <  1:3590906/150‑1 (MQ=255)
                 tGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCg                                                                                                                                      >  1:578176/1‑150 (MQ=255)
                 tGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCg                                                                                                                                      >  2:1249681/1‑150 (MQ=255)
                 tGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCg                                                                                                                                      <  2:2366173/150‑1 (MQ=255)
                   ggCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGaa                                                                                                                                    <  2:94898/150‑1 (MQ=255)
                   ggCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGaa                                                                                                                                    <  2:2408090/150‑1 (MQ=255)
                        ccTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGaaa                                                                                                                               <  1:2586563/150‑1 (MQ=255)
                          tGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAAc                                                                                                                             <  2:893879/150‑1 (MQ=255)
                            aCGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTc                                                                                                                           <  1:1075587/150‑1 (MQ=255)
                            aCGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTc                                                                                                                           <  1:4172091/150‑1 (MQ=255)
                             cggcggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCg                                                                                                                          <  1:1218843/150‑1 (MQ=255)
                                cggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGAtt                                                                                                                       >  1:3005780/1‑150 (MQ=255)
                                cggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGAtt                                                                                                                       <  2:4351180/150‑1 (MQ=255)
                                 ggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTa                                                                                                                      <  1:2372032/150‑1 (MQ=255)
                                    gCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCGGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTacac                                                                                                                   <  2:3465342/150‑1 (MQ=255)
                                      aaCTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCa                                                                                                                 >  1:991014/1‑150 (MQ=255)
                                       aCTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCAt                                                                                                                <  1:3765568/150‑1 (MQ=255)
                                           aCCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCt                                                                                                            <  2:786436/150‑1 (MQ=255)
                                              gTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGAt                                                                                                         <  1:3880910/150‑1 (MQ=255)
                                                tGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTc                                                                                                       <  2:3600857/150‑1 (MQ=255)
                                                tGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTc                                                                                                       <  2:1828840/150‑1 (MQ=255)
                                                 gCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCt                                                                                                      <  1:2485540/150‑1 (MQ=255)
                                                 gCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCt                                                                                                      <  1:3029880/150‑1 (MQ=255)
                                                  cACCGGGTCATTTGGTGGTTATCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTg                                                                                                     <  1:2140052/150‑1 (MQ=255)
                                                   aCCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCttt                                                                                                    <  1:4174380/150‑3 (MQ=255)
                                                      gggTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGc                                                                                                 >  1:407434/1‑150 (MQ=255)
                                                       ggTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGcc                                                                                                >  2:1094633/1‑150 (MQ=255)
                                                          cATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATc                                                                                             >  2:2447874/1‑150 (MQ=255)
                                                               ggtggtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCt                                                                                        <  1:815721/150‑1 (MQ=255)
                                                                    ttAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTc                                                                                   <  2:2697850/150‑1 (MQ=255)
                                                                      aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAg                                                                                 >  1:1301997/1‑150 (MQ=255)
                                                                      aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGAGTCTGTCGCCATCAGCCTGTTTCAg                                                                                 >  1:4341480/1‑150 (MQ=255)
                                                                      aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAg                                                                                 >  1:848001/1‑150 (MQ=255)
                                                                             tcCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTc                                                                          <  2:1294480/150‑1 (MQ=255)
                                                                             tcCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTc                                                                          <  2:731859/150‑1 (MQ=255)
                                                                              ccAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCa                                                                         <  1:3108734/150‑1 (MQ=255)
                                                                                aGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCAcc                                                                       >  1:2437462/1‑150 (MQ=255)
                                                                                 gACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCAccc                                                                      <  1:1318197/150‑1 (MQ=255)
                                                                                   cccTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCgg                                                                    >  2:592585/1‑150 (MQ=255)
                                                                                      tGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGc                                                                 >  2:4647450/1‑150 (MQ=255)
                                                                                       gaaagaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCt                                                                >  1:2079076/1‑150 (MQ=255)
                                                                                        aaagaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTc                                                               <  1:313848/150‑1 (MQ=255)
                                                                                        aaagaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTc                                                               >  1:1653268/1‑150 (MQ=255)
                                                                                         aagaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCg                                                              <  1:3620783/150‑1 (MQ=255)
                                                                                          agaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGc                                                             >  1:1684705/1‑150 (MQ=255)
                                                                                           gaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGcc                                                            >  1:489871/1‑150 (MQ=255)
                                                                                             aaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGt                                                          >  1:1115377/1‑150 (MQ=255)
                                                                                               cAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAg                                                        <  1:3264634/150‑1 (MQ=255)
                                                                                                aaaaTTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAgc                                                       >  2:1898149/1‑150 (MQ=255)
                                                                                                 aaaTTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAgcg                                                      >  1:76781/1‑150 (MQ=255)
                                                                                                   aTTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCt                                                    <  2:4517704/150‑1 (MQ=255)
                                                                                                    ttCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTc                                                   >  1:3142523/1‑150 (MQ=255)
                                                                                                    ttCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTc                                                   <  1:2524594/150‑1 (MQ=255)
                                                                                                      cccGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGa                                                 >  2:2925461/1‑150 (MQ=255)
                                                                                                             ttAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCAcc                                          <  2:1638236/150‑1 (MQ=255)
                                                                                                             ttAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCAcc                                          <  2:2063499/150‑1 (MQ=255)
                                                                                                              tAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCg                                         >  1:4587257/1‑150 (MQ=255)
                                                                                                              tAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCg                                         >  1:755280/1‑150 (MQ=255)
                                                                                                              tAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCg                                         >  2:2663228/1‑150 (MQ=255)
                                                                                                               aaGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGa                                        >  2:2620538/1‑150 (MQ=255)
                                                                                                                   tGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGaa                                    >  2:2304606/1‑150 (MQ=255)
                                                                                                                    ggAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGaac                                   <  2:807235/150‑1 (MQ=255)
                                                                                                                          tAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCgg                             <  1:2689443/150‑1 (MQ=255)
                                                                                                                          tAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCgg                             >  2:404065/1‑150 (MQ=255)
                                                                                                                            gacgacAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTg                           <  2:2995022/150‑1 (MQ=255)
                                                                                                                              cgacAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGAc                         <  2:633708/150‑1 (MQ=255)
                                                                                                                              cgacAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGAc                         >  1:1725332/1‑150 (MQ=255)
                                                                                                                              cgacAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGAc                         <  2:1365210/150‑1 (MQ=255)
                                                                                                                               gacAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACt                        <  2:4744544/150‑1 (MQ=255)
                                                                                                                               gacAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACt                        <  2:2030354/150‑1 (MQ=255)
                                                                                                                               gacAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACt                        <  1:4049288/150‑1 (MQ=255)
                                                                                                                                  aaaaaaGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTgg                     >  1:94772/1‑150 (MQ=255)
                                                                                                                                      aaGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTa                 >  2:1532074/1‑150 (MQ=255)
                                                                                                                                          ctctGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGccc             >  2:4080315/1‑150 (MQ=255)
                                                                                                                                          ctctGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGccc             >  1:18521/1‑150 (MQ=255)
                                                                                                                                          ctctGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGccc             >  2:3318581/1‑150 (MQ=255)
                                                                                                                                          ctctGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGccc             >  2:275273/1‑150 (MQ=255)
                                                                                                                                           tctGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCg            <  1:3266950/150‑1 (MQ=255)
                                                                                                                                            ctGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGt           >  1:4510096/1‑150 (MQ=255)
                                                                                                                                             tGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGtt          <  2:1314349/150‑1 (MQ=255)
                                                                                                                                               cGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAg        >  2:3115311/1‑150 (MQ=255)
                                                                                                                                                ggAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCTTTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGa       <  2:3649042/150‑1 (MQ=255)
                                                                                                                                                ggAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGa       >  1:1805823/1‑150 (MQ=255)
                                                                                                                                                 gaatgaatGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGAt      >  1:3230236/1‑150 (MQ=255)
                                                                                                                                                 gAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGAt      >  1:315082/1‑150 (MQ=255)
                                                                                                                                                   aTTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATgg    >  2:294097/1‑150 (MQ=255)
                                                                                                                                                   aTTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATgg    >  2:293933/1‑150 (MQ=255)
                                                                                                                                                   aTTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATgg    <  1:686659/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATGGCG  >  CP009273/2689573‑2689871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A