Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,689,722 T→G 20.1% L188* (TTA→TGA)  mltF → membrane‑bound lytic transglycosylase F, murein hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,689,7220TG20.1% 485.1 / 142.0 224L188* (TTA→TGA) mltFmembrane‑bound lytic transglycosylase F, murein hydrolase
Reads supporting (aligned to +/- strand):  ref base T (90/89);  new base G (29/16);  total (119/105)
Fisher's exact test for biased strand distribution p-value = 9.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 229 total aligned reads displayed.

TCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATGGC  >  CP009273/2689576‑2689870
                                                                                                                                                  |                                                                                                                                                    
tCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAAt                                                                                                                                                   >  2:5172262/1‑150 (MQ=255)
tCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAAt                                                                                                                                                   >  2:4472503/1‑150 (MQ=255)
  gCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATgg                                                                                                                                                 >  2:148790/1‑150 (MQ=255)
  gCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATgg                                                                                                                                                 >  1:1951843/1‑150 (MQ=255)
      aCGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTCGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGa                                                                                                                                             >  2:3938172/1‑150 (MQ=255)
       cGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGAt                                                                                                                                            <  2:4022352/150‑1 (MQ=255)
           cGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCa                                                                                                                                        <  1:1203094/150‑1 (MQ=255)
              tGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGACGATGTCATCg                                                                                                                                     >  2:3163290/1‑150 (MQ=255)
               gggCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGa                                                                                                                                    <  1:2558934/150‑1 (MQ=255)
                ggCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGaa                                                                                                                                   <  1:2678508/150‑1 (MQ=255)
                 gCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAg                                                                                                                                  >  1:4412626/1‑150 (MQ=255)
                  cAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGACTGAATGGAAGATGTCATCGAAgg                                                                                                                                 >  2:4873188/1‑150 (MQ=255)
                  cAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAgg                                                                                                                                 >  1:4996557/1‑150 (MQ=255)
                   aaCCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGa                                                                                                                                >  2:3098203/1‑150 (MQ=255)
                     ccTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGaaa                                                                                                                              >  2:3510953/1‑150 (MQ=255)
                     ccTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGaaa                                                                                                                              <  1:23015/150‑1 (MQ=255)
                     ccTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGaaa                                                                                                                              >  1:1715306/1‑150 (MQ=255)
                        gACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACt                                                                                                                           <  2:4096032/150‑1 (MQ=255)
                         aCGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTc                                                                                                                          >  1:3817254/1‑150 (MQ=255)
                              ggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTa                                                                                                                     >  2:2514627/1‑150 (MQ=255)
                               gAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTac                                                                                                                    <  2:4606698/150‑1 (MQ=255)
                               gAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTac                                                                                                                    <  2:3945329/150‑1 (MQ=255)
                                   aaCTCACCGTTTCACCGGGTAATGTGTTTTATAACGATATCAAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTTGAAGGTAGACGACAAAAAATGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCa                                                                                                                <  1:60240/150‑1 (MQ=255)
                                   aaCTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCa                                                                                                                <  1:32771/150‑1 (MQ=255)
                                    aCTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCAt                                                                                                               >  1:3226152/1‑150 (MQ=255)
                                    aCTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCAt                                                                                                               <  1:3502327/150‑1 (MQ=255)
                                    aCTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCAt                                                                                                               >  1:3226030/1‑150 (MQ=255)
                                    aCTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCAt                                                                                                               >  1:3066331/1‑150 (MQ=255)
                                      tCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTg                                                                                                             <  1:3610000/150‑1 (MQ=255)
                                          cGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGa                                                                                                         >  2:1839319/1‑150 (MQ=255)
                                             tGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTc                                                                                                      <  1:4070487/150‑1 (MQ=255)
                                             tGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTc                                                                                                      >  2:264135/1‑150 (MQ=255)
                                              gCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCt                                                                                                     <  2:726557/150‑1 (MQ=255)
                                               cACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTg                                                                                                    <  2:2160149/150‑1 (MQ=255)
                                                aCCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGt                                                                                                   <  1:5012439/150‑1 (MQ=255)
                                                aCCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGt                                                                                                   <  2:500911/150‑1 (MQ=255)
                                                    ggTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGcc                                                                                               >  2:1586647/1‑150 (MQ=255)
                                                       cATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATc                                                                                            >  1:4901240/1‑150 (MQ=255)
                                                       cATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATc                                                                                            >  2:3231845/1‑150 (MQ=255)
                                                        aTGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCa                                                                                           <  1:3997948/150‑1 (MQ=255)
                                                        aTGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCa                                                                                           <  1:1668184/150‑1 (MQ=255)
                                                          gtggtgGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGc                                                                                         >  1:1680007/1‑150 (MQ=255)
                                                           tggtggTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGcc                                                                                        >  1:3846519/1‑150 (MQ=255)
                                                            ggtggtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCt                                                                                       <  1:2893154/150‑1 (MQ=255)
                                                                gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGttt                                                                                   >  1:4562382/1‑150 (MQ=255)
                                                                  tAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCa                                                                                 >  2:1309106/1‑150 (MQ=255)
                                                                    aCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAgc                                                                               >  2:1086470/1‑150 (MQ=255)
                                                                     cGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAgcg                                                                              >  1:1805414/1‑150 (MQ=255)
                                                                     cGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAgcg                                                                              >  1:1105268/1‑150 (MQ=255)
                                                                       aTCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGcgcg                                                                            <  1:3169190/150‑1 (MQ=255)
                                                                       aTCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGcgcg                                                                            <  1:3370371/150‑1 (MQ=255)
                                                                          tcCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTc                                                                         >  2:1068105/1‑150 (MQ=255)
                                                                           ccAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCa                                                                        >  2:297996/1‑150 (MQ=255)
                                                                              gACCCTGAAAGAAACAAAAGTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCAccc                                                                     <  2:4388535/150‑1 (MQ=255)
                                                                                 ccTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGa                                                                  >  2:3741508/1‑150 (MQ=255)
                                                                                  cTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAg                                                                 >  2:4755700/1‑150 (MQ=255)
                                                                                    gaaagaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCt                                                               >  1:2845796/1‑150 (MQ=255)
                                                                                    gaaagaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCt                                                               >  1:2200551/1‑150 (MQ=255)
                                                                                    gaaagaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCt                                                               >  1:578939/1‑150 (MQ=255)
                                                                                     aaagaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTc                                                              >  2:3807819/1‑150 (MQ=255)
                                                                                     aaagaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTc                                                              >  2:536894/1‑150 (MQ=255)
                                                                                       agaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGc                                                            >  2:699309/1‑150 (MQ=255)
                                                                                        gaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGcc                                                           <  1:2434917/150‑1 (MQ=255)
                                                                                        gaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGcc                                                           >  1:2822295/1‑150 (MQ=255)
                                                                                            cAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAg                                                       <  1:1907200/150‑1 (MQ=255)
                                                                                            cAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAg                                                       >  1:239761/1‑150 (MQ=255)
                                                                                             aacaTTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAgc                                                      <  2:338875/147‑1 (MQ=255)
                                                                                                aTTCACGGAATTAAGCTTGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGATAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCt                                                   <  1:375123/150‑1 (MQ=255)
                                                                                                   cccGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCNa                                                <  1:251855/150‑1 (MQ=255)
                                                                                                   cccGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGa                                                <  1:3917168/150‑1 (MQ=255)
                                                                                                    ccGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGat                                               <  1:674774/150‑1 (MQ=255)
                                                                                                     cGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGata                                              >  1:4667652/1‑150 (MQ=255)
                                                                                                     cGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGata                                              >  1:1357440/1‑150 (MQ=255)
                                                                                                      ggAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGatat                                             <  1:136854/150‑1 (MQ=255)
                                                                                                      ggAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGatat                                             >  2:1626697/1‑150 (MQ=255)
                                                                                                          ttAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCAcc                                         >  2:1065033/1‑150 (MQ=255)
                                                                                                           tAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCg                                        <  2:1219403/150‑1 (MQ=255)
                                                                                                           tAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCg                                        >  1:1828773/1‑150 (MQ=255)
                                                                                                             aGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGAt                                      <  2:675386/150‑1 (MQ=255)
                                                                                                             aGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGAt                                      <  1:1547404/150‑1 (MQ=255)
                                                                                                                 ggAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGaac                                  >  2:4247911/1‑150 (MQ=255)
                                                                                                                      gTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCg                             >  2:5148702/1‑150 (MQ=255)
                                                                                                                         gacgacAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCGCGATATCACCGATGAACAACCGGTg                          >  2:1655225/1‑150 (MQ=255)
                                                                                                                             acAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACtt                      <  1:516982/150‑1 (MQ=255)
                                                                                                                              cAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATCTCACCGATGAACAACCGGTGACTTg                     >  1:3106665/1‑150 (MQ=255)
                                                                                                                                aaaaaGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGt                   >  1:5145972/1‑150 (MQ=255)
                                                                                                                                 aaaaGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGtt                  >  1:3777970/1‑150 (MQ=255)
                                                                                                                                 aaaaGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGtt                  <  2:2290836/150‑1 (MQ=255)
                                                                                                                                  aaaGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGttt                 <  2:42130/150‑1 (MQ=255)
                                                                                                                                  aaaGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGttt                 <  1:413881/150‑1 (MQ=255)
                                                                                                                                   aaGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTa                <  2:3333919/150‑1 (MQ=255)
                                                                                                                                   aaGGCTCTGCGGAATGAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTa                <  1:264135/150‑1 (MQ=255)
                                                                                                                                     ggCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGc              <  1:1484806/150‑1 (MQ=255)
                                                                                                                                     ggCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGc              <  2:54401/150‑1 (MQ=255)
                                                                                                                                          tGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGtt         <  2:3931819/150‑1 (MQ=255)
                                                                                                                                            cgtaatgaatgGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGTACAACCGGTGACTTGGTTTAGCCCGTTAg       <  1:4954700/147‑1 (MQ=255)
                                                                                                                                            cGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAg       <  1:77933/150‑1 (MQ=255)
                                                                                                                                              gAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGAt     >  1:2072399/1‑150 (MQ=255)
                                                                                                                                               aatgaatgGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATg    <  2:2943760/150‑1 (MQ=255)
                                                                                                                                                 ttAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATGGc  >  2:5008653/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTCACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATGGC  >  CP009273/2689576‑2689870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A