Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,245,848 G→T 84.8% L62L (CTG→CTT)  yhaH → DUF805 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,245,8480GT84.8% 597.3 / 116.7 245L62L (CTG→CTT) yhaHDUF805 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (22/15);  new base T (103/105);  total (125/120)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

Only 100 of 247 total aligned reads displayed.

GGTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAA  >  CP009273/3245699‑3245993
                                                                                                                                                     |                                                                                                                                                 
ggTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTg                                                                                                                                                   >  1:2399290/1‑150 (MQ=255)
  tttCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGACGGCATCCTGACGACAATCTACGGTATTCTTgt                                                                                                                                                 >  1:4670491/1‑150 (MQ=255)
   ttCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTtgtg                                                                                                                                                >  2:192460/1‑150 (MQ=255)
     ccGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGtt                                                                                                                                              <  2:1713676/150‑1 (MQ=255)
      cGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttt                                                                                                                                             <  1:4253681/150‑1 (MQ=255)
        gggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGttttt                                                                                                                                           <  2:2357473/150‑1 (MQ=255)
         ggTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTa                                                                                                                                          <  1:2909052/150‑1 (MQ=255)
          gTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTAc                                                                                                                                         <  2:3652217/150‑1 (MQ=255)
           tCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTAcc                                                                                                                                        >  1:3802860/1‑150 (MQ=255)
            cGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCg                                                                                                                                       <  2:3593742/150‑1 (MQ=255)
              tGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCgtg                                                                                                                                     <  2:2574651/150‑1 (MQ=255)
                  cggcgCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGtggtgg                                                                                                                                 <  2:2978715/150‑1 (MQ=255)
                       cAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGt                                                                                                                            >  2:2919486/1‑150 (MQ=255)
                        aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                           >  2:2443386/1‑150 (MQ=255)
                        aaaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTACTCTTGTGTTTTTACCGTGGTGGGCGGtt                                                                                                                           >  2:4097868/1‑150 (MQ=255)
                         aaGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCTTCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTc                                                                                                                          >  1:1467744/1‑150 (MQ=255)
                          agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCa                                                                                                                         >  2:4374541/1‑150 (MQ=255)
                          agagTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCa                                                                                                                         <  1:30544/150‑1 (MQ=255)
                            agTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGt                                                                                                                       >  1:199151/1‑150 (MQ=255)
                            agTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGt                                                                                                                       >  2:1029447/1‑150 (MQ=255)
                             gTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGtt                                                                                                                      <  1:252837/150‑1 (MQ=255)
                             gTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGtt                                                                                                                      <  2:2302387/150‑1 (MQ=255)
                                  ggATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgcc                                                                                                                 <  1:4346639/150‑1 (MQ=255)
                                   gATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgccg                                                                                                                >  1:1351778/1‑150 (MQ=255)
                                   gATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgccg                                                                                                                >  1:3413525/1‑150 (MQ=255)
                                     tGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCgccgcc                                                                                                              <  2:211224/150‑1 (MQ=255)
                                      gTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCt                                                                                                             >  1:3697075/1‑150 (MQ=255)
                                        ttATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGc                                                                                                           <  2:1953835/150‑1 (MQ=255)
                                              tGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGaca                                                                                                     >  2:166060/1‑150 (MQ=255)
                                               ggTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGacac                                                                                                    >  1:2886451/1‑150 (MQ=255)
                                                gTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACacc                                                                                                   >  2:2166876/1‑150 (MQ=255)
                                                           cTTTTCGTTCGTGCTGGGGCTGCTTTATAAAATGTTTGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTGTGGTGTTTTTACGGTGGTGGGCGTTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGc                                                                                        <  1:3677487/150‑1 (MQ=255)
                                                                cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                                                   >  1:2763398/1‑150 (MQ=255)
                                                                 gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  <  1:1004331/150‑1 (MQ=255)
                                                                 gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  >  2:3182691/1‑150 (MQ=255)
                                                                 gTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                                                  >  2:3180418/1‑150 (MQ=255)
                                                                    cGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGc                                                                               >  2:4068338/1‑150 (MQ=255)
                                                                     gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                                              >  1:2247962/1‑150 (MQ=255)
                                                                     gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                                              >  1:4627586/1‑150 (MQ=255)
                                                                     gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                                              >  2:2264832/1‑150 (MQ=255)
                                                                            ggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAt                                                                       >  2:2024255/1‑150 (MQ=255)
                                                                                 ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTa                                                                  <  2:4552967/150‑1 (MQ=255)
                                                                                 ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTa                                                                  <  1:2668455/150‑1 (MQ=255)
                                                                                   ggATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAt                                                                >  1:682176/1‑150 (MQ=255)
                                                                                     aTAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATcc                                                              >  2:2332625/1‑150 (MQ=255)
                                                                                      tAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATccc                                                             >  1:4186205/1‑150 (MQ=255)
                                                                                       aataTGTTAGGGGGGCATCGCGGTGGTGGCGATGGCATCGTGCCGACTATCGACGTTTTTCTTGTGTTTTTACCGTGGGGGGGGTTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            <  1:1592563/147‑1 (MQ=255)
                                                                                       aaatTGTTTGGCTGGCAAGGCGCTGGTGGCTAAGGCATCCTGACGACAATCTTCGGTATTCTTGTGTTTTTACGGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            <  1:3980590/150‑1 (MQ=255)
                                                                                       aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTTTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            <  1:3701601/150‑1 (MQ=255)
                                                                                       aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            >  2:2852356/1‑150 (MQ=255)
                                                                                       aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            >  2:477850/1‑150 (MQ=255)
                                                                                       aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCg                                                            <  2:11478/150‑1 (MQ=255)
                                                                                         aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGtt                                                          >  1:2659294/1‑150 (MQ=255)
                                                                                         aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGtt                                                          <  2:4167677/150‑1 (MQ=255)
                                                                                         aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGtt                                                          <  2:4167406/150‑1 (MQ=255)
                                                                                         aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGtt                                                          <  2:1782095/150‑1 (MQ=255)
                                                                                          aTGTTAGGCTGGCAACGCGCTGGTGGCTAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGttt                                                         <  2:1748430/150‑1 (MQ=255)
                                                                                          aTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGttt                                                         <  2:4656663/150‑1 (MQ=255)
                                                                                            gTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTAt                                                       >  2:2088656/1‑150 (MQ=255)
                                                                                              tAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                                     <  1:3770857/150‑1 (MQ=255)
                                                                                              tAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                                     <  2:2684931/150‑1 (MQ=255)
                                                                                               aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCgg                                                    <  2:2525538/150‑1 (MQ=255)
                                                                                                   tGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctgg                                                <  2:2242851/150‑1 (MQ=255)
                                                                                                      cAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCACGTTTATCGGCTGGCtt                                             >  1:1201082/1‑150 (MQ=255)
                                                                                                      cAACGCGCTGGGGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtt                                             <  2:903645/150‑1 (MQ=255)
                                                                                                        aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                                           <  2:2399290/150‑1 (MQ=255)
                                                                                                         cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                                          <  1:1038532/150‑1 (MQ=255)
                                                                                                         cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                                          <  2:1092346/150‑1 (MQ=255)
                                                                                                          gcgcTGGTGGCGAAGGCATCCTGACGACAATCTCCGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatta                                         <  1:1065606/150‑1 (MQ=255)
                                                                                                           cgcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTtattat                                        >  2:3987699/1‑150 (MQ=255)
                                                                                                           cgcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTtattat                                        >  2:3986941/1‑150 (MQ=255)
                                                                                                            gcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatc                                       >  2:3338115/1‑150 (MQ=255)
                                                                                                              tggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatcat                                     <  2:441428/150‑1 (MQ=255)
                                                                                                              tggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTGTATCGGCTGGCTTATTatcat                                     <  2:4044328/150‑1 (MQ=255)
                                                                                                               ggtggCGAAGGCATCCTGACTACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatcatc                                    >  1:3746281/1‑150 (MQ=255)
                                                                                                                 tggCGTAGGCATCCTGACGACAATCTCCGGTATTCTTGTGTTTGTACGGGGGGGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGt                                  <  1:2813253/150‑1 (MQ=255)
                                                                                                                   gCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCt                                <  1:2489451/150‑1 (MQ=255)
                                                                                                                     gAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCttt                              <  2:1351778/150‑1 (MQ=255)
                                                                                                                        ggCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAc                           <  1:4019072/150‑1 (MQ=255)
                                                                                                                         gCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt                          >  1:766192/1‑150 (MQ=255)
                                                                                                                          cATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTg                         <  2:3986409/150‑1 (MQ=255)
                                                                                                                          cATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTg                         >  1:4449830/1‑150 (MQ=255)
                                                                                                                           aTCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGc                        >  2:4161111/1‑150 (MQ=255)
                                                                                                                             ccTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa                      <  1:1765071/150‑1 (MQ=255)
                                                                                                                             ccTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa                      >  1:1856013/1‑150 (MQ=255)
                                                                                                                              cTGACGACAATCTACGTTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAg                     <  1:192460/150‑1 (MQ=255)
                                                                                                                               tGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAgg                    >  1:1981894/1‑150 (MQ=255)
                                                                                                                                 acgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCg                  >  2:2979320/1‑150 (MQ=255)
                                                                                                                                  cgacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCgg                 >  1:1168873/1‑150 (MQ=255)
                                                                                                                                   gacAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCggg                >  2:100627/1‑150 (MQ=255)
                                                                                                                                     cAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTa              >  1:717043/1‑150 (MQ=255)
                                                                                                                                      aaTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAc             >  2:2666634/1‑150 (MQ=255)
                                                                                                                                      aaTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAc             <  2:1272797/150‑1 (MQ=255)
                                                                                                                                      aaTCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAc             <  2:1272125/150‑1 (MQ=255)
                                                                                                                                       aTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACg            >  2:335742/1‑150 (MQ=255)
                                                                                                                                          tccGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCa         <  2:1072707/148‑1 (MQ=255)
                                                                                                                                              gTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCg     >  1:2537922/1‑150 (MQ=255)
                                                                                                                                              gTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACAGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCg     >  2:608547/1‑150 (MQ=255)
                                                                                                                                               tATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGa    >  1:4283031/1‑150 (MQ=255)
                                                                                                                                                aTTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGGTCCGCCGCCTGCTCGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGaa   <  2:4514426/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GGTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGAAA  >  CP009273/3245699‑3245993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGA  >  CP009273/3245699‑3245991
                                                                                                                                                     |                                                                                                                                               
GGTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTG                                                                                                                                                 >  SRR19973082.2414106/1‑150 (MQ=60)
  TTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGACGGCATCCTGACGACAATCTACGGTATTCTTGT                                                                                                                                               >  SRR19973082.4698701/1‑150 (MQ=60)
  TTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGT                                                                                                                                               >  SRR19973082.2303331/1‑150 (MQ=60)
    TCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGT                                                                                                                                             <  SRR19973082.1885079/150‑1 (MQ=60)
      CGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTT                                                                                                                                           <  SRR19973082.4279539/150‑1 (MQ=60)
      CGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTT                                                                                                                                           >  SRR19973082.3742377/1‑150 (MQ=60)
       GGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTT                                                                                                                                          <  SRR19973082.2052365/150‑1 (MQ=60)
         GGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTA                                                                                                                                        <  SRR19973082.2926940/150‑1 (MQ=60)
         GGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTA                                                                                                                                        <  SRR19973082.2927031/150‑1 (MQ=60)
          GTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTAC                                                                                                                                       <  SRR19973082.3335578/150‑1 (MQ=60)
           TCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACC                                                                                                                                      >  SRR19973082.3826333/1‑150 (MQ=60)
            CGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCG                                                                                                                                     >  SRR19973082.11664/1‑150 (MQ=60)
             GTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGT                                                                                                                                    >  SRR19973082.2541305/1‑150 (MQ=60)
               GCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGG                                                                                                                                  <  SRR19973082.3641673/150‑1 (MQ=60)
                  CGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCGGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGG                                                                                                                               <  SRR19973082.1950840/150‑1 (MQ=60)
                      GCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGG                                                                                                                           >  SRR19973082.2664875/1‑150 (MQ=60)
                      GCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGG                                                                                                                           >  SRR19973082.2663707/1‑150 (MQ=60)
                      GCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGG                                                                                                                           >  SRR19973082.3789197/1‑150 (MQ=60)
                         AAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCTTCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTC                                                                                                                        >  SRR19973082.1477712/1‑150 (MQ=60)
                         AAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCTTCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTC                                                                                                                        >  SRR19973082.1477708/1‑150 (MQ=60)
                         AAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCTTCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTC                                                                                                                        >  SRR19973082.1477636/1‑150 (MQ=60)
                         AAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTC                                                                                                                        <  SRR19973082.3702713/150‑1 (MQ=60)
                          AGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCA                                                                                                                       <  SRR19973082.30946/150‑1 (MQ=60)
                            AGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGT                                                                                                                     >  SRR19973082.200807/1‑150 (MQ=60)
                            AGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGT                                                                                                                     >  SRR19973082.2469357/1‑150 (MQ=60)
                             GTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTT                                                                                                                    <  SRR19973082.254774/150‑1 (MQ=60)
                                  GGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCC                                                                                                               <  SRR19973082.4372794/150‑1 (MQ=60)
                                  GGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCAGCC                                                                                                               >  SRR19973082.1032494/1‑150 (MQ=60)
                                  GGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCC                                                                                                               >  SRR19973082.1939482/1‑150 (MQ=60)
                                   GATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCG                                                                                                              >  SRR19973082.3434589/1‑150 (MQ=60)
                                   GATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCG                                                                                                              >  SRR19973082.1361091/1‑150 (MQ=60)
                                    ATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGC                                                                                                             >  SRR19973082.1767446/1‑150 (MQ=60)
                                    ATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGC                                                                                                             >  SRR19973082.3780174/1‑150 (MQ=60)
                                      GTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCT                                                                                                           >  SRR19973082.3719941/1‑150 (MQ=60)
                                               GGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACAC                                                                                                  >  SRR19973082.2904269/1‑150 (MQ=60)
                                                       TCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAATTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCTGTTCCGCCGCCTGCACGACACCGACCGCT                                                                                          <  SRR19973082.4137522/150‑1 (MQ=60)
                                                        CATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTC                                                                                         >  SRR19973082.1386199/1‑150 (MQ=60)
                                                           CTTTTCGTTCGTGCTGGGGCTGCTTTATAAAATGTTTGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTGTGGTGTTTTTACGGTGGTGGGCGTTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGC                                                                                      <  SRR19973082.3700217/150‑1 (MQ=60)
                                                           CTTTACGTTCGTGCTGGGGATGCTGTATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACTACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGC                                                                                      <  SRR19973082.1292357/150‑1 (MQ=60)
                                                             TTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGT                                                                                    >  SRR19973082.2859041/1‑150 (MQ=60)
                                                             TTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGT                                                                                    >  SRR19973082.2825235/1‑150 (MQ=60)
                                                              TACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTG                                                                                   >  SRR19973082.3045883/1‑150 (MQ=60)
                                                                CGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGT                                                                                 >  SRR19973082.2780004/1‑150 (MQ=60)
                                                                CGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGT                                                                                 >  SRR19973082.2780096/1‑150 (MQ=60)
                                                                 GTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTG                                                                                <  SRR19973082.1011323/150‑1 (MQ=60)
                                                                     GTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCA                                                                            >  SRR19973082.4655636/1‑150 (MQ=60)
                                                                     GTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCA                                                                            >  SRR19973082.2262099/1‑150 (MQ=60)
                                                                      TGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCAC                                                                           >  SRR19973082.748202/1‑150 (MQ=60)
                                                                       GCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACT                                                                          <  SRR19973082.3855909/150‑1 (MQ=60)
                                                                          GGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCTTCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCT                                                                       >  SRR19973082.947682/1‑150 (MQ=60)
                                                                              CTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAAACTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCTCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACAGCTCTTC                                                                   >  SRR19973082.4357370/1‑150 (MQ=60)
                                                                                 CTGGATAAAATGTTAGGCTGGCACCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTA                                                                <  SRR19973082.759807/150‑1 (MQ=60)
                                                                                 CTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTA                                                                <  SRR19973082.2684879/150‑1 (MQ=60)
                                                                                   GGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGTTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAT                                                              <  SRR19973082.535944/150‑1 (MQ=60)
                                                                                   GGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAT                                                              >  SRR19973082.686835/1‑150 (MQ=60)
                                                                                     ATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCC                                                            <  SRR19973082.3519003/150‑1 (MQ=60)
                                                                                      TAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCC                                                           <  SRR19973082.2733265/150‑1 (MQ=60)
                                                                                      TAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCC                                                           >  SRR19973082.4211795/1‑150 (MQ=60)
                                                                                       AATATGTTAGGGGGGCATCGCGGTGGTGGCGATGGCATCGTGCCGACTATCGACGTTTTTCTTGTGTTTTTACCGTGGGGGGGGTTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCG                                                          <  SRR19973082.1603025/150‑1 (MQ=60)
                                                                                       AAATTGTTTGGCTGGCAAGGCGCTGGTGGCTAAGGCATCCTGACGACAATCTTCGGTATTCTTGTGTTTTTACGGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCG                                                          <  SRR19973082.4004809/150‑1 (MQ=60)
                                                                                       AAATTGTTAGGCTGGCACCGCGGTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCG                                                          <  SRR19973082.4004770/150‑1 (MQ=60)
                                                                                       AAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTTTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCG                                                          <  SRR19973082.3724501/150‑1 (MQ=60)
                                                                                         AATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTT                                                        >  SRR19973082.2675685/1‑150 (MQ=60)
                                                                                         AATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTT                                                        >  SRR19973082.2675699/1‑150 (MQ=60)
                                                                                          ttgtgAGGCTGGCACCGCGCGGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACGGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTT                                                       <  SRR19973082.4578565/145‑1 (MQ=60)
                                                                                              TTGGCTGGCAAGGCGGTGGTGGCGAAGGCATCCTGACGACAATCTTCTGTATTCTTTTGTTTTTACCGTGGTGGGCGGTTCTGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCG                                                   <  SRR19973082.3794519/150‑1 (MQ=60)
                                                                                              TCGGCTGGCACCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTTTTCTTGTGTTTTTCCGGGGGGGGGCGGTTCTGGTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCG                                                   <  SRR19973082.456247/150‑1 (MQ=60)
                                                                                              TAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCG                                                   <  SRR19973082.3794146/150‑1 (MQ=60)
                                                                                               AGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGG                                                  <  SRR19973082.1318135/150‑1 (MQ=60)
                                                                                                      CAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCACGTTTATCGGCTGGCTT                                           >  SRR19973082.1209144/1‑150 (MQ=60)
                                                                                                       AACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTA                                          >  SRR19973082.4336900/1‑150 (MQ=60)
                                                                                                       AACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTA                                          >  SRR19973082.3014437/1‑150 (MQ=60)
                                                                                                        ACGCGCTGGTGGCGAAGGCATCCTGACTACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTAT                                         <  SRR19973082.3722942/150‑1 (MQ=60)
                                                                                                        ACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTAT                                         <  SRR19973082.3723054/150‑1 (MQ=60)
                                                                                                         CGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATT                                        <  SRR19973082.1045705/150‑1 (MQ=60)
                                                                                                          GCGCTGGTGGCGAAGGCATCCTGACGACAATCTCCGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTA                                       <  SRR19973082.1072918/150‑1 (MQ=60)
                                                                                                          GCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTA                                       <  SRR19973082.1072692/150‑1 (MQ=60)
                                                                                                          GCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTA                                       <  SRR19973082.2848247/150‑1 (MQ=60)
                                                                                                            GCTGGTGGCGAAGGCATCCTGACGACAATCTACGTTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATC                                     <  SRR19973082.4287099/150‑1 (MQ=60)
                                                                                                             CTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCA                                    >  SRR19973082.2021159/1‑150 (MQ=60)
                                                                                                               GGTGGCGAAGGCATCCTGACTACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATC                                  >  SRR19973082.3769445/1‑150 (MQ=60)
                                                                                                                 TGGCGTAGGCATCCTGACGACAATCTCCGGTATTCTTGTGTTTGTACGGGGGGGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGT                                <  SRR19973082.2830449/150‑1 (MQ=60)
                                                                                                                   GCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCT                              <  SRR19973082.2504916/150‑1 (MQ=60)
                                                                                                                   GCGAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCT                              <  SRR19973082.2504994/150‑1 (MQ=60)
                                                                                                                    CGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTT                             <  SRR19973082.4746720/150‑1 (MQ=60)
                                                                                                                     GAAGGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTT                            >  SRR19973082.1197660/1‑150 (MQ=60)
                                                                                                                        GGCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAC                         <  SRR19973082.4043675/150‑1 (MQ=60)
                                                                                                                         GCATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACT                        >  SRR19973082.771146/1‑150 (MQ=60)
                                                                                                                          CATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTG                       <  SRR19973082.4670295/150‑1 (MQ=60)
                                                                                                                          CATCCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTG                       >  SRR19973082.4476855/1‑150 (MQ=60)
                                                                                                                             CCTGACGACAATCTCCGGTTTTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCA                    <  SRR19973082.1776911/150‑1 (MQ=60)
                                                                                                                             CCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCA                    >  SRR19973082.1868057/1‑150 (MQ=60)
                                                                                                                             CCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCA                    <  SRR19973082.1776742/150‑1 (MQ=60)
                                                                                                                             CCTGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCA                    >  SRR19973082.4264069/1‑150 (MQ=60)
                                                                                                                              CTGACGACAATCTACGTTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAG                   <  SRR19973082.194083/150‑1 (MQ=60)
                                                                                                                               TGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACTACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCTGCTGGCTTATTATCATCGTCTTTAACTGCCAGG                  >  SRR19973082.1828353/1‑150 (MQ=60)
                                                                                                                               TGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGG                  >  SRR19973082.1994296/1‑150 (MQ=60)
                                                                                                                               TGACGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGG                  >  SRR19973082.1994852/1‑150 (MQ=60)
                                                                                                                                  CGACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGG               >  SRR19973082.1176527/1‑150 (MQ=60)
                                                                                                                                   GACAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGG              >  SRR19973082.4445516/1‑150 (MQ=60)
                                                                                                                                     CAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTA            >  SRR19973082.721840/1‑150 (MQ=60)
                                                                                                                                     CAATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTA            >  SRR19973082.114054/1‑150 (MQ=60)
                                                                                                                                      AATCTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAC           >  SRR19973082.4227615/1‑150 (MQ=60)
                                                                                                                                      AATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTAC           <  SRR19973082.4019061/150‑1 (MQ=60)
                                                                                                                                         CTACGGTATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCC        >  SRR19973082.690057/1‑150 (MQ=60)
                                                                                                                                              GTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCG   >  SRR19973082.2553762/1‑150 (MQ=60)
                                                                                                                                              GTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCG   >  SRR19973082.2553122/1‑150 (MQ=60)
                                                                                                                                               TATTCTTGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGA  >  SRR19973082.4308991/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                               
GGTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCGGGTACGCCAGGCGA  >  CP009273/3245699‑3245991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑